Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00435 and EX31_RS00440 are separated by 250 nucleotides EX31_RS00440 and EX31_RS00445 are separated by 214 nucleotides
EX31_RS00435: EX31_RS00435 - carbohydrate porin, at 92,743 to 94,323
_RS00435
EX31_RS00440: EX31_RS00440 - aminoimidazole riboside kinase, at 94,574 to 95,512
_RS00440
EX31_RS00445: EX31_RS00445 - outer membrane protein assembly factor BamA, at 95,727 to 98,150
_RS00445
Position (kb)
94
95
96 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 93.727 kb on + strand, within EX31_RS00435 at 93.727 kb on + strand, within EX31_RS00435 at 93.727 kb on + strand, within EX31_RS00435 at 93.727 kb on + strand, within EX31_RS00435 at 93.758 kb on + strand, within EX31_RS00435 at 93.758 kb on + strand, within EX31_RS00435 at 93.758 kb on + strand, within EX31_RS00435 at 93.758 kb on + strand, within EX31_RS00435 at 93.791 kb on + strand, within EX31_RS00435 at 93.848 kb on + strand, within EX31_RS00435 at 93.849 kb on - strand, within EX31_RS00435 at 93.849 kb on - strand, within EX31_RS00435 at 93.849 kb on - strand, within EX31_RS00435 at 93.849 kb on - strand, within EX31_RS00435 at 93.912 kb on - strand, within EX31_RS00435 at 93.968 kb on + strand, within EX31_RS00435 at 93.982 kb on - strand, within EX31_RS00435 at 94.009 kb on - strand, within EX31_RS00435 at 94.158 kb on + strand, within EX31_RS00435 at 94.159 kb on - strand, within EX31_RS00435 at 94.201 kb on + strand at 94.281 kb on + strand at 94.347 kb on - strand at 94.404 kb on - strand at 94.414 kb on + strand at 94.422 kb on + strand at 94.423 kb on - strand at 94.533 kb on + strand at 94.548 kb on + strand at 94.549 kb on - strand at 94.714 kb on + strand, within EX31_RS00440 at 94.784 kb on - strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.792 kb on + strand, within EX31_RS00440 at 94.793 kb on - strand, within EX31_RS00440 at 94.793 kb on - strand, within EX31_RS00440 at 94.793 kb on - strand, within EX31_RS00440 at 94.793 kb on - strand, within EX31_RS00440 at 94.793 kb on - strand, within EX31_RS00440 at 94.793 kb on - strand, within EX31_RS00440 at 94.796 kb on + strand, within EX31_RS00440 at 94.816 kb on + strand, within EX31_RS00440 at 94.817 kb on - strand, within EX31_RS00440 at 94.893 kb on + strand, within EX31_RS00440 at 94.893 kb on + strand, within EX31_RS00440 at 94.894 kb on - strand, within EX31_RS00440 at 94.947 kb on + strand, within EX31_RS00440 at 94.947 kb on + strand, within EX31_RS00440 at 94.947 kb on + strand, within EX31_RS00440 at 94.948 kb on - strand, within EX31_RS00440 at 95.027 kb on + strand, within EX31_RS00440 at 95.027 kb on + strand, within EX31_RS00440 at 95.027 kb on + strand, within EX31_RS00440 at 95.027 kb on + strand, within EX31_RS00440 at 95.028 kb on - strand, within EX31_RS00440 at 95.028 kb on - strand, within EX31_RS00440 at 95.028 kb on - strand, within EX31_RS00440 at 95.028 kb on - strand, within EX31_RS00440 at 95.028 kb on - strand, within EX31_RS00440 at 95.116 kb on + strand, within EX31_RS00440 at 95.204 kb on + strand, within EX31_RS00440 at 95.206 kb on + strand, within EX31_RS00440 at 95.206 kb on + strand, within EX31_RS00440 at 95.266 kb on + strand, within EX31_RS00440 at 95.314 kb on + strand, within EX31_RS00440 at 95.561 kb on + strand at 95.566 kb on + strand at 95.567 kb on - strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.627 kb on + strand at 95.628 kb on - strand at 95.628 kb on - strand at 95.628 kb on - strand at 95.628 kb on - strand at 95.629 kb on + strand at 95.629 kb on + strand at 95.629 kb on + strand at 95.629 kb on + strand at 95.629 kb on + strand at 95.629 kb on + strand at 95.630 kb on - strand at 95.630 kb on - strand at 95.630 kb on - strand at 95.630 kb on - strand at 95.630 kb on - strand at 95.631 kb on + strand at 95.631 kb on + strand at 95.631 kb on + strand at 95.631 kb on + strand at 95.631 kb on + strand at 95.632 kb on - strand at 95.632 kb on - strand at 95.632 kb on - strand at 95.656 kb on - strand at 95.675 kb on + strand at 95.749 kb on + strand at 96.018 kb on - strand, within EX31_RS00445 at 96.018 kb on - strand, within EX31_RS00445 at 96.178 kb on + strand, within EX31_RS00445 at 96.281 kb on + strand, within EX31_RS00445 at 96.290 kb on + strand, within EX31_RS00445 at 96.322 kb on + strand, within EX31_RS00445 at 96.323 kb on - strand, within EX31_RS00445 at 96.441 kb on - strand, within EX31_RS00445 at 96.489 kb on + strand, within EX31_RS00445 at 96.489 kb on + strand, within EX31_RS00445 at 96.489 kb on + strand, within EX31_RS00445 at 96.489 kb on + strand, within EX31_RS00445 at 96.492 kb on - strand, within EX31_RS00445 at 96.503 kb on + strand, within EX31_RS00445 at 96.506 kb on + strand, within EX31_RS00445 at 96.506 kb on + strand, within EX31_RS00445
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 93,727 + EX31_RS00435 0.62 -1.1 93,727 + EX31_RS00435 0.62 -0.3 93,727 + EX31_RS00435 0.62 +0.5 93,727 + EX31_RS00435 0.62 +0.8 93,758 + EX31_RS00435 0.64 +0.1 93,758 + EX31_RS00435 0.64 -1.2 93,758 + EX31_RS00435 0.64 +0.1 93,758 + EX31_RS00435 0.64 +0.4 93,791 + EX31_RS00435 0.66 -1.7 93,848 + EX31_RS00435 0.70 +0.3 93,849 - EX31_RS00435 0.70 -1.8 93,849 - EX31_RS00435 0.70 -0.6 93,849 - EX31_RS00435 0.70 +0.5 93,849 - EX31_RS00435 0.70 -0.0 93,912 - EX31_RS00435 0.74 +1.6 93,968 + EX31_RS00435 0.77 +0.8 93,982 - EX31_RS00435 0.78 +1.9 94,009 - EX31_RS00435 0.80 +0.2 94,158 + EX31_RS00435 0.90 +0.5 94,159 - EX31_RS00435 0.90 -0.6 94,201 + +0.6 94,281 + +1.8 94,347 - +0.1 94,404 - -0.6 94,414 + +0.6 94,422 + +0.2 94,423 - -0.5 94,533 + +0.5 94,548 + +1.0 94,549 - -0.5 94,714 + EX31_RS00440 0.15 -0.3 94,784 - EX31_RS00440 0.22 -0.9 94,792 + EX31_RS00440 0.23 -0.9 94,792 + EX31_RS00440 0.23 +0.6 94,792 + EX31_RS00440 0.23 +0.8 94,792 + EX31_RS00440 0.23 +0.4 94,792 + EX31_RS00440 0.23 -1.4 94,792 + EX31_RS00440 0.23 -0.0 94,792 + EX31_RS00440 0.23 -0.2 94,792 + EX31_RS00440 0.23 -0.9 94,792 + EX31_RS00440 0.23 +0.0 94,792 + EX31_RS00440 0.23 -0.6 94,792 + EX31_RS00440 0.23 +0.2 94,792 + EX31_RS00440 0.23 -1.7 94,792 + EX31_RS00440 0.23 +1.4 94,792 + EX31_RS00440 0.23 -0.1 94,793 - EX31_RS00440 0.23 +0.2 94,793 - EX31_RS00440 0.23 +1.4 94,793 - EX31_RS00440 0.23 -0.3 94,793 - EX31_RS00440 0.23 -1.3 94,793 - EX31_RS00440 0.23 +0.2 94,793 - EX31_RS00440 0.23 +0.2 94,796 + EX31_RS00440 0.24 +0.2 94,816 + EX31_RS00440 0.26 -0.5 94,817 - EX31_RS00440 0.26 +0.0 94,893 + EX31_RS00440 0.34 -2.3 94,893 + EX31_RS00440 0.34 -0.3 94,894 - EX31_RS00440 0.34 +0.8 94,947 + EX31_RS00440 0.40 +0.6 94,947 + EX31_RS00440 0.40 +0.4 94,947 + EX31_RS00440 0.40 -0.0 94,948 - EX31_RS00440 0.40 -0.0 95,027 + EX31_RS00440 0.48 +0.5 95,027 + EX31_RS00440 0.48 -0.2 95,027 + EX31_RS00440 0.48 +0.6 95,027 + EX31_RS00440 0.48 +0.3 95,028 - EX31_RS00440 0.48 +0.6 95,028 - EX31_RS00440 0.48 -1.0 95,028 - EX31_RS00440 0.48 -2.1 95,028 - EX31_RS00440 0.48 -1.3 95,028 - EX31_RS00440 0.48 +0.0 95,116 + EX31_RS00440 0.58 -0.2 95,204 + EX31_RS00440 0.67 +0.0 95,206 + EX31_RS00440 0.67 -0.6 95,206 + EX31_RS00440 0.67 -0.0 95,266 + EX31_RS00440 0.74 +0.7 95,314 + EX31_RS00440 0.79 -0.0 95,561 + +2.0 95,566 + +0.1 95,567 - -0.3 95,627 + -0.7 95,627 + -0.7 95,627 + -0.2 95,627 + -1.7 95,627 + -1.6 95,627 + -1.3 95,627 + -0.5 95,627 + +0.3 95,627 + -0.5 95,628 - -0.1 95,628 - +0.2 95,628 - +1.4 95,628 - +0.7 95,629 + +0.4 95,629 + +0.3 95,629 + -0.2 95,629 + -1.2 95,629 + +1.0 95,629 + -0.3 95,630 - -1.3 95,630 - +1.2 95,630 - -0.2 95,630 - +0.0 95,630 - -0.7 95,631 + -1.9 95,631 + +1.3 95,631 + +0.2 95,631 + +0.2 95,631 + -1.2 95,632 - +0.6 95,632 - +1.3 95,632 - -1.0 95,656 - +0.4 95,675 + -0.2 95,749 + -0.5 96,018 - EX31_RS00445 0.12 -0.6 96,018 - EX31_RS00445 0.12 +1.2 96,178 + EX31_RS00445 0.19 +1.7 96,281 + EX31_RS00445 0.23 +1.2 96,290 + EX31_RS00445 0.23 -0.4 96,322 + EX31_RS00445 0.25 -0.6 96,323 - EX31_RS00445 0.25 -1.3 96,441 - EX31_RS00445 0.29 -0.5 96,489 + EX31_RS00445 0.31 +1.5 96,489 + EX31_RS00445 0.31 -0.0 96,489 + EX31_RS00445 0.31 +0.4 96,489 + EX31_RS00445 0.31 +1.2 96,492 - EX31_RS00445 0.32 -1.6 96,503 + EX31_RS00445 0.32 +0.9 96,506 + EX31_RS00445 0.32 +1.5 96,506 + EX31_RS00445 0.32 +0.3
Or see this region's nucleotide sequence