Experiment: Vancomycin 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS09130 and AZOBR_RS09135 overlap by 32 nucleotides AZOBR_RS09135 and AZOBR_RS09140 are separated by 260 nucleotides AZOBR_RS09140 and AZOBR_RS09145 are separated by 56 nucleotides AZOBR_RS09145 and AZOBR_RS09150 are separated by 13 nucleotides
AZOBR_RS09130: AZOBR_RS09130 - conserved membrane protein of unknown function, at 1,908,008 to 1,908,946
_RS09130
AZOBR_RS09135: AZOBR_RS09135 - GCN5 family acetyltransferase, at 1,908,915 to 1,909,355
_RS09135
AZOBR_RS09140: AZOBR_RS09140 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 1,909,616 to 1,910,920
_RS09140
AZOBR_RS09145: AZOBR_RS09145 - hypothetical protein, at 1,910,977 to 1,911,441
_RS09145
AZOBR_RS09150: AZOBR_RS09150 - peroxiredoxin, at 1,911,455 to 1,911,874
_RS09150
Position (kb)
1909
1910
1911 Strain fitness (log2 ratio)
-2
-1
0
1 at 1908.645 kb on - strand, within AZOBR_RS09130 at 1908.720 kb on - strand at 1908.729 kb on - strand, within AZOBR_RS09130 at 1909.015 kb on + strand, within AZOBR_RS09135 at 1909.040 kb on - strand, within AZOBR_RS09135 at 1909.045 kb on + strand, within AZOBR_RS09135 at 1909.046 kb on - strand, within AZOBR_RS09135 at 1909.046 kb on - strand, within AZOBR_RS09135 at 1909.150 kb on + strand, within AZOBR_RS09135 at 1909.151 kb on - strand, within AZOBR_RS09135 at 1909.151 kb on - strand, within AZOBR_RS09135 at 1909.159 kb on + strand, within AZOBR_RS09135 at 1909.159 kb on + strand, within AZOBR_RS09135 at 1909.396 kb on - strand at 1909.472 kb on + strand at 1911.878 kb on + strand at 1911.878 kb on + strand at 1911.893 kb on + strand at 1911.894 kb on - strand at 1911.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.03 mg/ml remove 1,908,645 - AZOBR_RS09130 0.68 +0.3 1,908,720 - +0.3 1,908,729 - AZOBR_RS09130 0.77 +0.2 1,909,015 + AZOBR_RS09135 0.23 +0.2 1,909,040 - AZOBR_RS09135 0.28 -0.6 1,909,045 + AZOBR_RS09135 0.29 -0.0 1,909,046 - AZOBR_RS09135 0.30 -1.2 1,909,046 - AZOBR_RS09135 0.30 -0.7 1,909,150 + AZOBR_RS09135 0.53 -0.0 1,909,151 - AZOBR_RS09135 0.54 -0.8 1,909,151 - AZOBR_RS09135 0.54 +0.5 1,909,159 + AZOBR_RS09135 0.55 -0.5 1,909,159 + AZOBR_RS09135 0.55 +0.5 1,909,396 - -2.2 1,909,472 + +0.1 1,911,878 + -0.2 1,911,878 + +0.2 1,911,893 + +0.0 1,911,894 - +0.3 1,911,894 - -0.1
Or see this region's nucleotide sequence