Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3517 and Ac3H11_3518 are separated by 110 nucleotides Ac3H11_3518 and Ac3H11_3519 overlap by 17 nucleotides Ac3H11_3519 and Ac3H11_3520 overlap by 16 nucleotides
Ac3H11_3517 - Transcriptional regulator, LysR family, at 62,991 to 63,893
_3517
Ac3H11_3518 - Rrf2-linked NADH-flavin reductase, at 64,004 to 64,645
_3518
Ac3H11_3519 - hypothetical protein, at 64,629 to 64,796
_3519
Ac3H11_3520 - TRAP-type C4-dicarboxylate transport system, large permease component, at 64,781 to 66,061
_3520
Position (kb) 64
65 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.023 kb on - strand at 63.023 kb on - strand at 63.023 kb on - strand at 63.406 kb on + strand, within Ac3H11_3517 at 63.406 kb on + strand, within Ac3H11_3517 at 63.407 kb on - strand, within Ac3H11_3517 at 63.407 kb on - strand, within Ac3H11_3517 at 63.407 kb on - strand, within Ac3H11_3517 at 63.899 kb on - strand at 63.943 kb on - strand at 63.965 kb on + strand at 63.977 kb on - strand at 64.211 kb on + strand, within Ac3H11_3518 at 64.211 kb on + strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.698 kb on + strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519 at 64.996 kb on - strand, within Ac3H11_3520 at 65.493 kb on + strand, within Ac3H11_3520
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 63,023 - +0.6 63,023 - -1.1 63,023 - +0.3 63,406 + Ac3H11_3517 0.46 +0.6 63,406 + Ac3H11_3517 0.46 -0.7 63,407 - Ac3H11_3517 0.46 +2.1 63,407 - Ac3H11_3517 0.46 -0.2 63,407 - Ac3H11_3517 0.46 -0.4 63,899 - -1.1 63,943 - +0.2 63,965 + -0.2 63,977 - +0.5 64,211 + Ac3H11_3518 0.32 -1.9 64,211 + Ac3H11_3518 0.32 +0.8 64,212 - Ac3H11_3518 0.32 +0.9 64,212 - Ac3H11_3518 0.32 +0.1 64,212 - Ac3H11_3518 0.32 -0.9 64,698 + Ac3H11_3519 0.41 -0.1 64,717 - Ac3H11_3519 0.52 -0.9 64,717 - Ac3H11_3519 0.52 -0.5 64,996 - Ac3H11_3520 0.17 -0.5 65,493 + Ac3H11_3520 0.56 -1.4
Or see this region's nucleotide sequence