Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS27080 and AZOBR_RS27085 are separated by 184 nucleotides
AZOBR_RS27080: AZOBR_RS27080 - transcriptional regulator, at 222,431 to 223,336
_RS27080
AZOBR_RS27085: AZOBR_RS27085 - branched-chain amino acid ABC transporter substrate-binding protein, at 223,521 to 224,531
_RS27085
Position (kb) 223
224
225 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 222.591 kb on + strand, within AZOBR_RS27080 at 222.592 kb on - strand, within AZOBR_RS27080 at 222.592 kb on - strand, within AZOBR_RS27080 at 222.729 kb on + strand, within AZOBR_RS27080 at 222.729 kb on + strand, within AZOBR_RS27080 at 222.730 kb on - strand, within AZOBR_RS27080 at 223.213 kb on - strand, within AZOBR_RS27080 at 223.373 kb on - strand at 223.512 kb on + strand at 223.575 kb on + strand at 223.575 kb on + strand at 223.576 kb on - strand at 223.576 kb on - strand at 223.576 kb on - strand at 223.666 kb on - strand, within AZOBR_RS27085 at 223.666 kb on - strand, within AZOBR_RS27085 at 223.762 kb on - strand, within AZOBR_RS27085 at 223.762 kb on - strand, within AZOBR_RS27085 at 223.824 kb on + strand, within AZOBR_RS27085 at 223.824 kb on + strand, within AZOBR_RS27085 at 223.891 kb on - strand, within AZOBR_RS27085 at 223.891 kb on - strand, within AZOBR_RS27085 at 224.001 kb on + strand, within AZOBR_RS27085 at 224.001 kb on + strand, within AZOBR_RS27085 at 224.002 kb on - strand, within AZOBR_RS27085 at 224.002 kb on - strand, within AZOBR_RS27085 at 224.605 kb on + strand at 224.605 kb on + strand at 224.606 kb on - strand at 224.848 kb on + strand at 224.848 kb on + strand at 225.262 kb on + strand at 225.262 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 222,591 + AZOBR_RS27080 0.18 +0.1 222,592 - AZOBR_RS27080 0.18 -0.2 222,592 - AZOBR_RS27080 0.18 -0.4 222,729 + AZOBR_RS27080 0.33 +0.5 222,729 + AZOBR_RS27080 0.33 +0.7 222,730 - AZOBR_RS27080 0.33 -0.6 223,213 - AZOBR_RS27080 0.86 -0.1 223,373 - -0.2 223,512 + -0.1 223,575 + +1.3 223,575 + +0.0 223,576 - -1.2 223,576 - -0.7 223,576 - -0.2 223,666 - AZOBR_RS27085 0.14 -0.8 223,666 - AZOBR_RS27085 0.14 -0.7 223,762 - AZOBR_RS27085 0.24 -1.0 223,762 - AZOBR_RS27085 0.24 -0.6 223,824 + AZOBR_RS27085 0.30 -0.6 223,824 + AZOBR_RS27085 0.30 -0.1 223,891 - AZOBR_RS27085 0.37 +1.1 223,891 - AZOBR_RS27085 0.37 -0.1 224,001 + AZOBR_RS27085 0.47 -3.5 224,001 + AZOBR_RS27085 0.47 -0.3 224,002 - AZOBR_RS27085 0.48 +1.3 224,002 - AZOBR_RS27085 0.48 -1.2 224,605 + +0.0 224,605 + +0.3 224,606 - -0.4 224,848 + +0.5 224,848 + +0.1 225,262 + -0.1 225,262 + +0.3
Or see this region's nucleotide sequence