Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse2; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00500 and MMP_RS00505 are separated by 4 nucleotides MMP_RS00505 and MMP_RS00510 are separated by 14 nucleotides MMP_RS00510 and MMP_RS00515 are separated by 20 nucleotides MMP_RS00515 and MMP_RS00520 are separated by 100 nucleotides
MMP_RS00500: MMP_RS00500 - glutamyl-tRNA reductase, at 98,977 to 100,125
_RS00500
MMP_RS00505: MMP_RS00505 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 100,130 to 100,735
_RS00505
MMP_RS00510: MMP_RS00510 - glycosyltransferase family 4 protein, at 100,750 to 101,817
_RS00510
MMP_RS00515: MMP_RS00515 - DUF655 domain-containing protein, at 101,838 to 102,422
_RS00515
MMP_RS00520: MMP_RS00520 - RNA polymerase Rpb4 family protein, at 102,523 to 102,846
_RS00520
Position (kb)
100
101
102 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 100.089 kb on - strand at 100.771 kb on + strand at 100.774 kb on - strand at 100.781 kb on - strand at 101.209 kb on + strand at 101.318 kb on - strand at 101.319 kb on + strand at 101.612 kb on - strand at 101.729 kb on + strand at 101.793 kb on + strand at 101.793 kb on + strand at 101.795 kb on + strand at 102.041 kb on - strand, within MMP_RS00515 at 102.335 kb on - strand, within MMP_RS00515 at 102.373 kb on - strand at 102.379 kb on - strand at 102.454 kb on + strand at 102.460 kb on + strand at 102.463 kb on - strand at 102.463 kb on - strand at 102.479 kb on - strand at 102.541 kb on - strand at 102.622 kb on - strand, within MMP_RS00520 at 102.706 kb on - strand, within MMP_RS00520 at 102.758 kb on + strand, within MMP_RS00520
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse2; 20 generations remove 100,089 - -4.9 100,771 + -5.2 100,774 - -3.1 100,781 - -4.1 101,209 + -1.7 101,318 - -5.0 101,319 + -4.2 101,612 - -4.5 101,729 + -4.0 101,793 + -5.4 101,793 + -4.1 101,795 + -5.0 102,041 - MMP_RS00515 0.35 -4.4 102,335 - MMP_RS00515 0.85 -4.4 102,373 - -4.6 102,379 - -4.7 102,454 + -4.3 102,460 + -4.5 102,463 - -3.7 102,463 - -4.5 102,479 - -3.0 102,541 - +0.1 102,622 - MMP_RS00520 0.31 -0.9 102,706 - MMP_RS00520 0.56 -3.3 102,758 + MMP_RS00520 0.73 -2.9
Or see this region's nucleotide sequence