Experiment: Sucrose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05480 and HMPREF1058_RS05475 are separated by 279 nucleotides HMPREF1058_RS05475 and HMPREF1058_RS05470 are separated by 24 nucleotides
HMPREF1058_RS05480: HMPREF1058_RS05480 - helix-turn-helix domain-containing protein, at 155,999 to 156,919
_RS05480
HMPREF1058_RS05475: HMPREF1058_RS05475 - lactonase family protein, at 157,199 to 158,353
_RS05475
HMPREF1058_RS05470: HMPREF1058_RS05470 - carboxymuconolactone decarboxylase family protein, at 158,378 to 159,985
_RS05470
Position (kb) 157
158
159 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 156.265 kb on - strand, within HMPREF1058_RS05480 at 156.265 kb on - strand, within HMPREF1058_RS05480 at 156.307 kb on + strand, within HMPREF1058_RS05480 at 156.309 kb on + strand, within HMPREF1058_RS05480 at 156.370 kb on + strand, within HMPREF1058_RS05480 at 156.430 kb on + strand, within HMPREF1058_RS05480 at 156.491 kb on - strand, within HMPREF1058_RS05480 at 156.491 kb on - strand, within HMPREF1058_RS05480 at 156.491 kb on - strand, within HMPREF1058_RS05480 at 156.578 kb on - strand, within HMPREF1058_RS05480 at 156.581 kb on + strand, within HMPREF1058_RS05480 at 156.582 kb on - strand, within HMPREF1058_RS05480 at 156.889 kb on + strand at 156.890 kb on - strand at 156.894 kb on - strand at 156.903 kb on - strand at 156.904 kb on + strand at 156.905 kb on - strand at 156.941 kb on - strand at 156.949 kb on - strand at 156.999 kb on + strand at 157.023 kb on + strand at 157.046 kb on - strand at 157.119 kb on + strand at 157.143 kb on - strand at 157.191 kb on + strand at 157.196 kb on - strand at 157.196 kb on - strand at 157.322 kb on + strand, within HMPREF1058_RS05475 at 157.322 kb on + strand, within HMPREF1058_RS05475 at 157.322 kb on + strand, within HMPREF1058_RS05475 at 157.324 kb on + strand, within HMPREF1058_RS05475 at 157.325 kb on - strand, within HMPREF1058_RS05475 at 157.429 kb on + strand, within HMPREF1058_RS05475 at 157.575 kb on - strand at 157.577 kb on - strand, within HMPREF1058_RS05475 at 157.668 kb on + strand, within HMPREF1058_RS05475 at 157.669 kb on - strand, within HMPREF1058_RS05475 at 157.804 kb on + strand, within HMPREF1058_RS05475 at 157.804 kb on + strand, within HMPREF1058_RS05475 at 157.804 kb on + strand, within HMPREF1058_RS05475 at 157.804 kb on + strand, within HMPREF1058_RS05475 at 157.805 kb on - strand, within HMPREF1058_RS05475 at 157.915 kb on + strand, within HMPREF1058_RS05475 at 157.915 kb on + strand at 157.915 kb on + strand, within HMPREF1058_RS05475 at 157.915 kb on + strand, within HMPREF1058_RS05475 at 157.915 kb on + strand, within HMPREF1058_RS05475 at 157.916 kb on - strand, within HMPREF1058_RS05475 at 157.916 kb on - strand, within HMPREF1058_RS05475 at 157.918 kb on - strand, within HMPREF1058_RS05475 at 158.090 kb on + strand, within HMPREF1058_RS05475 at 158.179 kb on + strand, within HMPREF1058_RS05475 at 158.260 kb on - strand at 158.377 kb on + strand at 158.378 kb on - strand at 158.389 kb on - strand at 158.393 kb on - strand at 158.420 kb on - strand at 158.452 kb on + strand at 158.458 kb on - strand at 158.495 kb on + strand at 158.495 kb on + strand at 158.495 kb on + strand at 158.584 kb on - strand, within HMPREF1058_RS05470 at 158.598 kb on - strand, within HMPREF1058_RS05470 at 158.725 kb on + strand, within HMPREF1058_RS05470 at 158.726 kb on - strand, within HMPREF1058_RS05470 at 158.845 kb on - strand, within HMPREF1058_RS05470 at 158.991 kb on + strand, within HMPREF1058_RS05470 at 159.027 kb on + strand, within HMPREF1058_RS05470 at 159.028 kb on - strand, within HMPREF1058_RS05470 at 159.101 kb on - strand, within HMPREF1058_RS05470 at 159.110 kb on + strand, within HMPREF1058_RS05470 at 159.110 kb on + strand, within HMPREF1058_RS05470 at 159.111 kb on - strand, within HMPREF1058_RS05470
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 20 mM (C) remove 156,265 - HMPREF1058_RS05480 0.29 -1.4 156,265 - HMPREF1058_RS05480 0.29 -0.4 156,307 + HMPREF1058_RS05480 0.33 -0.9 156,309 + HMPREF1058_RS05480 0.34 -0.7 156,370 + HMPREF1058_RS05480 0.40 +0.5 156,430 + HMPREF1058_RS05480 0.47 -1.9 156,491 - HMPREF1058_RS05480 0.53 -1.0 156,491 - HMPREF1058_RS05480 0.53 +1.1 156,491 - HMPREF1058_RS05480 0.53 +1.6 156,578 - HMPREF1058_RS05480 0.63 +1.9 156,581 + HMPREF1058_RS05480 0.63 +1.4 156,582 - HMPREF1058_RS05480 0.63 -2.9 156,889 + -0.7 156,890 - -0.1 156,894 - +0.4 156,903 - +0.6 156,904 + +2.2 156,905 - -1.6 156,941 - -1.1 156,949 - -0.6 156,999 + -0.6 157,023 + -1.7 157,046 - -1.4 157,119 + -1.0 157,143 - +1.4 157,191 + +0.4 157,196 - +1.2 157,196 - -0.3 157,322 + HMPREF1058_RS05475 0.11 -1.1 157,322 + HMPREF1058_RS05475 0.11 +0.6 157,322 + HMPREF1058_RS05475 0.11 +0.5 157,324 + HMPREF1058_RS05475 0.11 -1.1 157,325 - HMPREF1058_RS05475 0.11 -0.1 157,429 + HMPREF1058_RS05475 0.20 -0.2 157,575 - +0.9 157,577 - HMPREF1058_RS05475 0.33 -0.7 157,668 + HMPREF1058_RS05475 0.41 -1.1 157,669 - HMPREF1058_RS05475 0.41 -0.1 157,804 + HMPREF1058_RS05475 0.52 -0.3 157,804 + HMPREF1058_RS05475 0.52 -0.4 157,804 + HMPREF1058_RS05475 0.52 -1.7 157,804 + HMPREF1058_RS05475 0.52 -0.6 157,805 - HMPREF1058_RS05475 0.52 -0.6 157,915 + HMPREF1058_RS05475 0.62 -1.1 157,915 + +0.1 157,915 + HMPREF1058_RS05475 0.62 -2.0 157,915 + HMPREF1058_RS05475 0.62 +0.1 157,915 + HMPREF1058_RS05475 0.62 -1.0 157,916 - HMPREF1058_RS05475 0.62 -0.1 157,916 - HMPREF1058_RS05475 0.62 -3.1 157,918 - HMPREF1058_RS05475 0.62 +0.7 158,090 + HMPREF1058_RS05475 0.77 +0.0 158,179 + HMPREF1058_RS05475 0.85 +0.4 158,260 - -0.8 158,377 + -1.5 158,378 - -0.1 158,389 - -2.8 158,393 - -1.4 158,420 - +5.3 158,452 + -1.3 158,458 - -0.6 158,495 + -0.7 158,495 + +1.8 158,495 + -2.6 158,584 - HMPREF1058_RS05470 0.13 -0.0 158,598 - HMPREF1058_RS05470 0.14 -0.1 158,725 + HMPREF1058_RS05470 0.22 +0.9 158,726 - HMPREF1058_RS05470 0.22 -0.5 158,845 - HMPREF1058_RS05470 0.29 -1.4 158,991 + HMPREF1058_RS05470 0.38 +1.1 159,027 + HMPREF1058_RS05470 0.40 -0.8 159,028 - HMPREF1058_RS05470 0.40 -1.1 159,101 - HMPREF1058_RS05470 0.45 -2.4 159,110 + HMPREF1058_RS05470 0.46 -2.8 159,110 + HMPREF1058_RS05470 0.46 -0.6 159,111 - HMPREF1058_RS05470 0.46 -1.6
Or see this region's nucleotide sequence