Experiment: Sucrose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03250 and HMPREF1058_RS03245 are separated by 219 nucleotides HMPREF1058_RS03245 and HMPREF1058_RS03240 are separated by 164 nucleotides HMPREF1058_RS03240 and HMPREF1058_RS03235 are separated by 129 nucleotides
HMPREF1058_RS03250: HMPREF1058_RS03250 - hypothetical protein, at 321,286 to 321,639
_RS03250
HMPREF1058_RS03245: HMPREF1058_RS03245 - thiamine phosphate synthase, at 321,859 to 322,467
_RS03245
HMPREF1058_RS03240: HMPREF1058_RS03240 - ribose-phosphate pyrophosphokinase, at 322,632 to 323,570
_RS03240
HMPREF1058_RS03235: HMPREF1058_RS03235 - ABC transporter substrate-binding protein, at 323,700 to 325,034
_RS03235
Position (kb) 322
323
324 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 321.678 kb on - strand at 321.705 kb on + strand at 321.705 kb on + strand at 321.705 kb on + strand at 321.706 kb on - strand at 321.761 kb on - strand at 321.814 kb on - strand at 321.817 kb on - strand at 321.822 kb on + strand at 321.823 kb on - strand at 321.823 kb on - strand at 321.830 kb on + strand at 321.847 kb on + strand at 321.859 kb on - strand at 321.866 kb on + strand at 321.866 kb on + strand at 321.915 kb on + strand at 322.015 kb on + strand, within HMPREF1058_RS03245 at 322.117 kb on + strand, within HMPREF1058_RS03245 at 322.187 kb on - strand, within HMPREF1058_RS03245 at 322.301 kb on + strand, within HMPREF1058_RS03245 at 322.478 kb on + strand at 322.511 kb on - strand at 322.529 kb on - strand at 322.532 kb on - strand at 323.559 kb on + strand at 323.559 kb on + strand at 323.560 kb on - strand at 323.569 kb on - strand at 323.575 kb on + strand at 323.628 kb on + strand at 323.629 kb on - strand at 323.634 kb on + strand at 323.638 kb on + strand at 323.676 kb on + strand at 323.782 kb on - strand at 323.784 kb on + strand at 323.785 kb on - strand at 323.785 kb on - strand at 323.847 kb on + strand, within HMPREF1058_RS03235 at 323.847 kb on + strand, within HMPREF1058_RS03235 at 323.940 kb on + strand, within HMPREF1058_RS03235 at 323.941 kb on - strand, within HMPREF1058_RS03235 at 323.945 kb on + strand, within HMPREF1058_RS03235 at 323.949 kb on + strand, within HMPREF1058_RS03235 at 323.950 kb on - strand, within HMPREF1058_RS03235 at 323.950 kb on - strand, within HMPREF1058_RS03235 at 323.950 kb on - strand, within HMPREF1058_RS03235 at 323.955 kb on + strand, within HMPREF1058_RS03235 at 323.957 kb on + strand, within HMPREF1058_RS03235 at 323.958 kb on - strand, within HMPREF1058_RS03235 at 323.961 kb on - strand, within HMPREF1058_RS03235 at 324.001 kb on + strand, within HMPREF1058_RS03235 at 324.001 kb on + strand, within HMPREF1058_RS03235 at 324.002 kb on - strand, within HMPREF1058_RS03235 at 324.011 kb on + strand, within HMPREF1058_RS03235 at 324.011 kb on + strand, within HMPREF1058_RS03235 at 324.012 kb on - strand, within HMPREF1058_RS03235 at 324.012 kb on - strand, within HMPREF1058_RS03235 at 324.014 kb on + strand, within HMPREF1058_RS03235 at 324.018 kb on + strand, within HMPREF1058_RS03235 at 324.085 kb on - strand, within HMPREF1058_RS03235 at 324.110 kb on - strand, within HMPREF1058_RS03235 at 324.117 kb on - strand, within HMPREF1058_RS03235 at 324.143 kb on + strand, within HMPREF1058_RS03235 at 324.148 kb on + strand, within HMPREF1058_RS03235 at 324.149 kb on - strand, within HMPREF1058_RS03235 at 324.149 kb on - strand, within HMPREF1058_RS03235 at 324.182 kb on + strand, within HMPREF1058_RS03235 at 324.183 kb on - strand, within HMPREF1058_RS03235 at 324.293 kb on + strand, within HMPREF1058_RS03235 at 324.470 kb on - strand, within HMPREF1058_RS03235
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose 20 mM (C) remove 321,678 - -0.8 321,705 + +2.0 321,705 + -1.9 321,705 + -0.7 321,706 - -0.5 321,761 - -1.0 321,814 - +1.0 321,817 - +0.3 321,822 + +1.0 321,823 - -1.3 321,823 - -0.2 321,830 + +1.7 321,847 + -1.0 321,859 - -2.2 321,866 + -1.1 321,866 + -0.7 321,915 + -1.3 322,015 + HMPREF1058_RS03245 0.26 -0.2 322,117 + HMPREF1058_RS03245 0.42 -1.8 322,187 - HMPREF1058_RS03245 0.54 -1.0 322,301 + HMPREF1058_RS03245 0.73 -0.5 322,478 + +0.9 322,511 - +2.8 322,529 - -1.8 322,532 - -1.3 323,559 + -2.1 323,559 + -3.9 323,560 - -3.5 323,569 - +0.3 323,575 + -0.7 323,628 + +0.3 323,629 - -1.3 323,634 + -1.1 323,638 + +0.8 323,676 + -0.5 323,782 - +0.8 323,784 + -1.0 323,785 - -0.4 323,785 - -1.5 323,847 + HMPREF1058_RS03235 0.11 -0.1 323,847 + HMPREF1058_RS03235 0.11 +0.7 323,940 + HMPREF1058_RS03235 0.18 +0.0 323,941 - HMPREF1058_RS03235 0.18 +0.9 323,945 + HMPREF1058_RS03235 0.18 -1.9 323,949 + HMPREF1058_RS03235 0.19 +0.7 323,950 - HMPREF1058_RS03235 0.19 +0.5 323,950 - HMPREF1058_RS03235 0.19 -0.3 323,950 - HMPREF1058_RS03235 0.19 +0.9 323,955 + HMPREF1058_RS03235 0.19 +1.6 323,957 + HMPREF1058_RS03235 0.19 -1.5 323,958 - HMPREF1058_RS03235 0.19 +0.9 323,961 - HMPREF1058_RS03235 0.20 +1.0 324,001 + HMPREF1058_RS03235 0.23 -2.2 324,001 + HMPREF1058_RS03235 0.23 +0.2 324,002 - HMPREF1058_RS03235 0.23 +0.3 324,011 + HMPREF1058_RS03235 0.23 -1.8 324,011 + HMPREF1058_RS03235 0.23 +0.6 324,012 - HMPREF1058_RS03235 0.23 +1.4 324,012 - HMPREF1058_RS03235 0.23 -0.3 324,014 + HMPREF1058_RS03235 0.24 -0.6 324,018 + HMPREF1058_RS03235 0.24 -2.3 324,085 - HMPREF1058_RS03235 0.29 +0.0 324,110 - HMPREF1058_RS03235 0.31 -1.5 324,117 - HMPREF1058_RS03235 0.31 +0.9 324,143 + HMPREF1058_RS03235 0.33 +2.5 324,148 + HMPREF1058_RS03235 0.34 -3.5 324,149 - HMPREF1058_RS03235 0.34 -0.2 324,149 - HMPREF1058_RS03235 0.34 -1.3 324,182 + HMPREF1058_RS03235 0.36 -1.0 324,183 - HMPREF1058_RS03235 0.36 -0.1 324,293 + HMPREF1058_RS03235 0.44 +1.2 324,470 - HMPREF1058_RS03235 0.58 +0.4
Or see this region's nucleotide sequence