Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23645 and EX31_RS23650 are separated by 82 nucleotides EX31_RS23650 and EX31_RS23655 are separated by 122 nucleotides EX31_RS23655 and EX31_RS23660 are separated by 37 nucleotides
EX31_RS23645: EX31_RS23645 - HAMP domain-containing protein, at 492,969 to 494,480
_RS23645
EX31_RS23650: EX31_RS23650 - putative acyl-CoA thioester hydrolase, at 494,563 to 495,843
_RS23650
EX31_RS23655: EX31_RS23655 - kinase inhibitor, at 495,966 to 496,448
_RS23655
EX31_RS23660: EX31_RS23660 - type 1 glutamine amidotransferase domain-containing protein, at 496,486 to 497,181
_RS23660
Position (kb) 494
495
496 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 493.625 kb on + strand, within EX31_RS23645 at 493.625 kb on + strand, within EX31_RS23645 at 493.762 kb on + strand, within EX31_RS23645 at 493.763 kb on - strand, within EX31_RS23645 at 493.772 kb on + strand, within EX31_RS23645 at 493.772 kb on + strand, within EX31_RS23645 at 493.773 kb on - strand, within EX31_RS23645 at 493.935 kb on - strand, within EX31_RS23645 at 493.973 kb on + strand, within EX31_RS23645 at 494.004 kb on - strand, within EX31_RS23645 at 494.039 kb on + strand, within EX31_RS23645 at 494.178 kb on - strand, within EX31_RS23645 at 494.178 kb on - strand, within EX31_RS23645 at 494.479 kb on - strand at 494.564 kb on + strand at 494.565 kb on - strand at 494.565 kb on - strand at 494.565 kb on - strand at 494.594 kb on + strand at 494.595 kb on - strand at 494.617 kb on - strand at 494.617 kb on - strand at 494.617 kb on - strand at 494.717 kb on + strand, within EX31_RS23650 at 494.717 kb on + strand, within EX31_RS23650 at 494.717 kb on + strand, within EX31_RS23650 at 494.717 kb on + strand, within EX31_RS23650 at 494.717 kb on + strand, within EX31_RS23650 at 494.718 kb on - strand, within EX31_RS23650 at 494.718 kb on - strand, within EX31_RS23650 at 494.718 kb on - strand, within EX31_RS23650 at 494.718 kb on - strand, within EX31_RS23650 at 494.747 kb on - strand, within EX31_RS23650 at 494.968 kb on + strand, within EX31_RS23650 at 494.969 kb on - strand, within EX31_RS23650 at 494.969 kb on - strand, within EX31_RS23650 at 494.969 kb on - strand, within EX31_RS23650 at 494.972 kb on + strand, within EX31_RS23650 at 494.973 kb on - strand, within EX31_RS23650 at 494.973 kb on - strand, within EX31_RS23650 at 494.973 kb on - strand, within EX31_RS23650 at 494.973 kb on - strand, within EX31_RS23650 at 495.000 kb on + strand, within EX31_RS23650 at 495.001 kb on - strand, within EX31_RS23650 at 495.001 kb on - strand, within EX31_RS23650 at 495.001 kb on - strand, within EX31_RS23650 at 495.001 kb on - strand, within EX31_RS23650 at 495.008 kb on + strand, within EX31_RS23650 at 495.008 kb on + strand, within EX31_RS23650 at 495.008 kb on + strand, within EX31_RS23650 at 495.008 kb on + strand, within EX31_RS23650 at 495.008 kb on + strand, within EX31_RS23650 at 495.008 kb on + strand, within EX31_RS23650 at 495.008 kb on + strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.009 kb on - strand, within EX31_RS23650 at 495.020 kb on + strand, within EX31_RS23650 at 495.067 kb on - strand, within EX31_RS23650 at 495.067 kb on - strand, within EX31_RS23650 at 495.166 kb on - strand, within EX31_RS23650 at 495.288 kb on + strand, within EX31_RS23650 at 495.288 kb on + strand, within EX31_RS23650 at 495.288 kb on + strand, within EX31_RS23650 at 495.288 kb on + strand, within EX31_RS23650 at 495.289 kb on - strand, within EX31_RS23650 at 495.289 kb on - strand, within EX31_RS23650 at 495.289 kb on - strand, within EX31_RS23650 at 495.289 kb on - strand, within EX31_RS23650 at 495.289 kb on - strand, within EX31_RS23650 at 495.289 kb on - strand, within EX31_RS23650 at 495.289 kb on - strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.290 kb on + strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.291 kb on - strand, within EX31_RS23650 at 495.419 kb on - strand, within EX31_RS23650 at 495.449 kb on + strand, within EX31_RS23650 at 495.450 kb on - strand, within EX31_RS23650 at 495.450 kb on - strand, within EX31_RS23650 at 495.450 kb on - strand, within EX31_RS23650 at 495.450 kb on - strand, within EX31_RS23650 at 495.464 kb on + strand, within EX31_RS23650 at 495.464 kb on + strand, within EX31_RS23650 at 495.464 kb on + strand, within EX31_RS23650 at 495.464 kb on + strand, within EX31_RS23650 at 495.464 kb on + strand, within EX31_RS23650 at 495.465 kb on - strand, within EX31_RS23650 at 495.465 kb on - strand, within EX31_RS23650 at 495.465 kb on - strand, within EX31_RS23650 at 495.465 kb on - strand, within EX31_RS23650 at 495.465 kb on - strand, within EX31_RS23650 at 495.492 kb on + strand, within EX31_RS23650 at 495.492 kb on + strand, within EX31_RS23650 at 495.492 kb on + strand, within EX31_RS23650 at 495.492 kb on + strand, within EX31_RS23650 at 495.492 kb on + strand, within EX31_RS23650 at 495.493 kb on - strand, within EX31_RS23650 at 495.493 kb on - strand, within EX31_RS23650 at 495.493 kb on - strand, within EX31_RS23650 at 495.547 kb on - strand, within EX31_RS23650 at 495.551 kb on + strand, within EX31_RS23650 at 495.552 kb on - strand, within EX31_RS23650 at 495.572 kb on - strand, within EX31_RS23650 at 495.677 kb on + strand, within EX31_RS23650 at 495.690 kb on + strand, within EX31_RS23650 at 495.690 kb on + strand, within EX31_RS23650 at 495.690 kb on + strand, within EX31_RS23650 at 495.691 kb on - strand, within EX31_RS23650 at 495.810 kb on + strand at 495.810 kb on + strand at 495.811 kb on - strand at 495.811 kb on - strand at 495.811 kb on - strand at 495.953 kb on + strand at 495.953 kb on + strand at 495.953 kb on + strand at 495.954 kb on - strand at 495.954 kb on - strand at 495.954 kb on - strand at 495.954 kb on - strand at 495.954 kb on - strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.973 kb on + strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.974 kb on - strand at 495.975 kb on + strand at 495.975 kb on + strand at 495.975 kb on + strand at 495.976 kb on - strand at 495.976 kb on - strand at 496.004 kb on + strand at 496.004 kb on + strand at 496.005 kb on - strand at 496.031 kb on - strand, within EX31_RS23655 at 496.081 kb on + strand, within EX31_RS23655 at 496.081 kb on + strand, within EX31_RS23655 at 496.081 kb on + strand, within EX31_RS23655 at 496.082 kb on - strand, within EX31_RS23655 at 496.083 kb on + strand, within EX31_RS23655 at 496.084 kb on - strand, within EX31_RS23655 at 496.096 kb on + strand, within EX31_RS23655 at 496.097 kb on - strand, within EX31_RS23655 at 496.182 kb on + strand, within EX31_RS23655 at 496.182 kb on + strand, within EX31_RS23655 at 496.238 kb on + strand, within EX31_RS23655 at 496.291 kb on + strand, within EX31_RS23655 at 496.369 kb on + strand, within EX31_RS23655 at 496.427 kb on + strand at 496.439 kb on + strand at 496.439 kb on + strand at 496.440 kb on - strand at 496.440 kb on - strand at 496.448 kb on + strand at 496.448 kb on + strand at 496.448 kb on + strand at 496.448 kb on + strand at 496.449 kb on - strand at 496.449 kb on - strand at 496.449 kb on - strand at 496.488 kb on - strand at 496.488 kb on - strand at 496.488 kb on - strand at 496.523 kb on - strand at 496.523 kb on - strand at 496.614 kb on + strand, within EX31_RS23660 at 496.614 kb on + strand, within EX31_RS23660 at 496.615 kb on - strand, within EX31_RS23660 at 496.701 kb on + strand, within EX31_RS23660 at 496.701 kb on + strand, within EX31_RS23660 at 496.701 kb on + strand, within EX31_RS23660 at 496.702 kb on - strand, within EX31_RS23660 at 496.704 kb on + strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 493,625 + EX31_RS23645 0.43 +0.5 493,625 + EX31_RS23645 0.43 +0.5 493,762 + EX31_RS23645 0.52 +0.6 493,763 - EX31_RS23645 0.53 +0.1 493,772 + EX31_RS23645 0.53 +1.4 493,772 + EX31_RS23645 0.53 -0.1 493,773 - EX31_RS23645 0.53 +0.3 493,935 - EX31_RS23645 0.64 -0.8 493,973 + EX31_RS23645 0.66 +2.4 494,004 - EX31_RS23645 0.68 -1.2 494,039 + EX31_RS23645 0.71 +0.2 494,178 - EX31_RS23645 0.80 -1.9 494,178 - EX31_RS23645 0.80 +0.7 494,479 - +1.5 494,564 + -0.0 494,565 - -0.3 494,565 - -0.9 494,565 - -0.4 494,594 + -0.5 494,595 - +0.4 494,617 - +1.1 494,617 - +0.3 494,617 - -0.9 494,717 + EX31_RS23650 0.12 +0.4 494,717 + EX31_RS23650 0.12 +0.4 494,717 + EX31_RS23650 0.12 +0.7 494,717 + EX31_RS23650 0.12 -1.4 494,717 + EX31_RS23650 0.12 +0.7 494,718 - EX31_RS23650 0.12 +1.8 494,718 - EX31_RS23650 0.12 -0.4 494,718 - EX31_RS23650 0.12 -0.9 494,718 - EX31_RS23650 0.12 -1.5 494,747 - EX31_RS23650 0.14 +0.1 494,968 + EX31_RS23650 0.32 -0.0 494,969 - EX31_RS23650 0.32 -0.5 494,969 - EX31_RS23650 0.32 -0.0 494,969 - EX31_RS23650 0.32 -1.0 494,972 + EX31_RS23650 0.32 -0.5 494,973 - EX31_RS23650 0.32 +0.2 494,973 - EX31_RS23650 0.32 -0.0 494,973 - EX31_RS23650 0.32 +0.2 494,973 - EX31_RS23650 0.32 -0.1 495,000 + EX31_RS23650 0.34 +0.5 495,001 - EX31_RS23650 0.34 +1.0 495,001 - EX31_RS23650 0.34 +0.5 495,001 - EX31_RS23650 0.34 +0.3 495,001 - EX31_RS23650 0.34 +1.1 495,008 + EX31_RS23650 0.35 +0.3 495,008 + EX31_RS23650 0.35 +2.0 495,008 + EX31_RS23650 0.35 +0.2 495,008 + EX31_RS23650 0.35 -0.8 495,008 + EX31_RS23650 0.35 +0.4 495,008 + EX31_RS23650 0.35 +1.0 495,008 + EX31_RS23650 0.35 -0.8 495,009 - EX31_RS23650 0.35 -0.7 495,009 - EX31_RS23650 0.35 -0.7 495,009 - EX31_RS23650 0.35 +0.1 495,009 - EX31_RS23650 0.35 +1.2 495,009 - EX31_RS23650 0.35 -1.9 495,009 - EX31_RS23650 0.35 -1.4 495,009 - EX31_RS23650 0.35 -0.1 495,009 - EX31_RS23650 0.35 -0.0 495,009 - EX31_RS23650 0.35 -1.3 495,009 - EX31_RS23650 0.35 +0.0 495,020 + EX31_RS23650 0.36 +0.7 495,067 - EX31_RS23650 0.39 +0.5 495,067 - EX31_RS23650 0.39 -0.6 495,166 - EX31_RS23650 0.47 +0.8 495,288 + EX31_RS23650 0.57 -0.9 495,288 + EX31_RS23650 0.57 -0.4 495,288 + EX31_RS23650 0.57 -0.7 495,288 + EX31_RS23650 0.57 -2.2 495,289 - EX31_RS23650 0.57 -0.0 495,289 - EX31_RS23650 0.57 -0.4 495,289 - EX31_RS23650 0.57 -0.0 495,289 - EX31_RS23650 0.57 -0.0 495,289 - EX31_RS23650 0.57 +1.4 495,289 - EX31_RS23650 0.57 +0.5 495,289 - EX31_RS23650 0.57 +0.6 495,290 + EX31_RS23650 0.57 +0.7 495,290 + EX31_RS23650 0.57 +3.0 495,290 + EX31_RS23650 0.57 -0.4 495,290 + EX31_RS23650 0.57 +0.6 495,290 + EX31_RS23650 0.57 -1.0 495,290 + EX31_RS23650 0.57 -0.6 495,290 + EX31_RS23650 0.57 -1.1 495,290 + EX31_RS23650 0.57 +0.0 495,290 + EX31_RS23650 0.57 -0.8 495,290 + EX31_RS23650 0.57 +2.2 495,290 + EX31_RS23650 0.57 -0.8 495,290 + EX31_RS23650 0.57 -2.6 495,290 + EX31_RS23650 0.57 +0.7 495,291 - EX31_RS23650 0.57 +1.0 495,291 - EX31_RS23650 0.57 +0.4 495,291 - EX31_RS23650 0.57 +0.6 495,291 - EX31_RS23650 0.57 +0.2 495,291 - EX31_RS23650 0.57 -0.8 495,291 - EX31_RS23650 0.57 +0.6 495,291 - EX31_RS23650 0.57 -0.1 495,291 - EX31_RS23650 0.57 -0.2 495,291 - EX31_RS23650 0.57 +0.6 495,291 - EX31_RS23650 0.57 -0.9 495,419 - EX31_RS23650 0.67 -0.9 495,449 + EX31_RS23650 0.69 +0.6 495,450 - EX31_RS23650 0.69 +0.9 495,450 - EX31_RS23650 0.69 +1.0 495,450 - EX31_RS23650 0.69 +1.0 495,450 - EX31_RS23650 0.69 +0.7 495,464 + EX31_RS23650 0.70 +1.1 495,464 + EX31_RS23650 0.70 -0.9 495,464 + EX31_RS23650 0.70 -2.8 495,464 + EX31_RS23650 0.70 -1.0 495,464 + EX31_RS23650 0.70 -1.2 495,465 - EX31_RS23650 0.70 +1.4 495,465 - EX31_RS23650 0.70 -0.3 495,465 - EX31_RS23650 0.70 -0.3 495,465 - EX31_RS23650 0.70 +1.0 495,465 - EX31_RS23650 0.70 +0.2 495,492 + EX31_RS23650 0.73 +0.1 495,492 + EX31_RS23650 0.73 +0.1 495,492 + EX31_RS23650 0.73 +1.2 495,492 + EX31_RS23650 0.73 +2.2 495,492 + EX31_RS23650 0.73 +2.0 495,493 - EX31_RS23650 0.73 +1.2 495,493 - EX31_RS23650 0.73 -1.2 495,493 - EX31_RS23650 0.73 -0.8 495,547 - EX31_RS23650 0.77 -1.5 495,551 + EX31_RS23650 0.77 -2.4 495,552 - EX31_RS23650 0.77 -0.3 495,572 - EX31_RS23650 0.79 +2.0 495,677 + EX31_RS23650 0.87 -0.4 495,690 + EX31_RS23650 0.88 -1.0 495,690 + EX31_RS23650 0.88 +0.8 495,690 + EX31_RS23650 0.88 +1.0 495,691 - EX31_RS23650 0.88 +1.0 495,810 + +0.7 495,810 + +0.6 495,811 - +0.2 495,811 - -1.3 495,811 - +0.2 495,953 + +1.0 495,953 + -1.2 495,953 + +1.2 495,954 - -0.5 495,954 - +0.1 495,954 - +0.6 495,954 - +0.1 495,954 - -0.0 495,973 + -0.5 495,973 + +0.2 495,973 + -0.4 495,973 + +1.3 495,973 + -0.5 495,973 + -0.1 495,973 + -0.0 495,973 + +0.1 495,973 + +0.3 495,973 + +2.0 495,973 + +0.8 495,973 + -1.5 495,973 + +0.5 495,973 + +0.2 495,974 - +0.4 495,974 - -0.1 495,974 - +0.7 495,974 - -0.2 495,974 - +0.6 495,974 - -1.2 495,974 - -0.2 495,974 - -0.2 495,974 - -1.3 495,974 - -0.2 495,974 - -0.0 495,975 + -0.5 495,975 + -0.8 495,975 + -0.7 495,976 - -0.2 495,976 - +0.6 496,004 + +1.1 496,004 + -0.9 496,005 - +0.5 496,031 - EX31_RS23655 0.13 +0.3 496,081 + EX31_RS23655 0.24 +1.3 496,081 + EX31_RS23655 0.24 -0.4 496,081 + EX31_RS23655 0.24 -0.4 496,082 - EX31_RS23655 0.24 -0.5 496,083 + EX31_RS23655 0.24 -0.8 496,084 - EX31_RS23655 0.24 +2.0 496,096 + EX31_RS23655 0.27 +1.6 496,097 - EX31_RS23655 0.27 -0.2 496,182 + EX31_RS23655 0.45 +0.2 496,182 + EX31_RS23655 0.45 +0.7 496,238 + EX31_RS23655 0.56 +1.3 496,291 + EX31_RS23655 0.67 +0.4 496,369 + EX31_RS23655 0.83 -0.3 496,427 + +1.4 496,439 + +1.3 496,439 + +1.2 496,440 - -0.2 496,440 - -0.4 496,448 + +0.3 496,448 + -0.1 496,448 + -0.4 496,448 + +0.7 496,449 - +0.8 496,449 - +1.1 496,449 - +0.5 496,488 - +1.0 496,488 - -1.5 496,488 - +1.2 496,523 - -0.1 496,523 - +1.0 496,614 + EX31_RS23660 0.18 -0.5 496,614 + EX31_RS23660 0.18 +0.6 496,615 - EX31_RS23660 0.19 -0.6 496,701 + EX31_RS23660 0.31 +1.1 496,701 + EX31_RS23660 0.31 -0.6 496,701 + EX31_RS23660 0.31 +0.2 496,702 - EX31_RS23660 0.31 -0.4 496,704 + EX31_RS23660 0.31 -0.6 496,705 - EX31_RS23660 0.31 +0.2 496,705 - EX31_RS23660 0.31 +0.5 496,705 - EX31_RS23660 0.31 -0.4 496,705 - EX31_RS23660 0.31 +0.2
Or see this region's nucleotide sequence