Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23415 and EX31_RS23420 are separated by 827 nucleotides EX31_RS23420 and EX31_RS23425 overlap by 7 nucleotides EX31_RS23425 and EX31_RS23430 are separated by 3 nucleotides
EX31_RS23415: EX31_RS23415 - citrate synthase, at 448,628 to 449,911
_RS23415
EX31_RS23420: EX31_RS23420 - succinate dehydrogenase cytochrome b556 subunit, at 450,739 to 451,128
_RS23420
EX31_RS23425: EX31_RS23425 - succinate dehydrogenase membrane anchor subunit, at 451,122 to 451,469
_RS23425
EX31_RS23430: EX31_RS23430 - succinate dehydrogenase flavoprotein subunit, at 451,473 to 453,239
_RS23430
Position (kb) 450
451
452 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 449.764 kb on + strand, within EX31_RS23415 at 449.785 kb on - strand at 449.888 kb on - strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.989 kb on + strand at 449.990 kb on - strand at 449.992 kb on + strand at 449.993 kb on - strand at 449.993 kb on - strand at 450.005 kb on - strand at 450.005 kb on - strand at 450.033 kb on + strand at 450.033 kb on + strand at 450.034 kb on - strand at 450.116 kb on + strand at 450.117 kb on - strand at 450.135 kb on - strand at 450.147 kb on + strand at 450.274 kb on + strand at 450.274 kb on + strand at 450.275 kb on - strand at 450.275 kb on - strand at 450.296 kb on + strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.304 kb on + strand at 450.304 kb on + strand at 450.305 kb on - strand at 450.382 kb on + strand at 450.382 kb on + strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.451 kb on + strand at 450.451 kb on + strand at 450.453 kb on + strand at 450.459 kb on + strand at 450.459 kb on + strand at 450.484 kb on + strand at 450.485 kb on - strand at 450.535 kb on + strand at 450.562 kb on + strand at 450.609 kb on + strand at 450.609 kb on + strand at 450.609 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.703 kb on - strand at 450.824 kb on + strand, within EX31_RS23420 at 450.825 kb on - strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.936 kb on + strand, within EX31_RS23420 at 450.936 kb on + strand, within EX31_RS23420 at 450.936 kb on + strand, within EX31_RS23420 at 450.985 kb on + strand, within EX31_RS23420 at 451.004 kb on + strand, within EX31_RS23420 at 451.126 kb on + strand at 451.151 kb on + strand at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.208 kb on + strand, within EX31_RS23425 at 451.208 kb on + strand, within EX31_RS23425 at 451.209 kb on - strand, within EX31_RS23425 at 451.217 kb on + strand, within EX31_RS23425 at 451.217 kb on + strand, within EX31_RS23425 at 451.217 kb on + strand, within EX31_RS23425 at 451.297 kb on + strand, within EX31_RS23425 at 451.297 kb on + strand, within EX31_RS23425 at 451.368 kb on + strand, within EX31_RS23425 at 451.431 kb on + strand, within EX31_RS23425 at 451.431 kb on + strand, within EX31_RS23425 at 451.435 kb on + strand at 451.435 kb on + strand at 451.440 kb on + strand at 451.467 kb on + strand at 451.467 kb on + strand at 451.467 kb on + strand at 451.467 kb on + strand at 451.607 kb on + strand at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.705 kb on - strand, within EX31_RS23430 at 451.706 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.732 kb on - strand, within EX31_RS23430 at 451.732 kb on - strand, within EX31_RS23430 at 451.732 kb on - strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.815 kb on + strand, within EX31_RS23430 at 451.815 kb on + strand, within EX31_RS23430 at 451.816 kb on - strand, within EX31_RS23430 at 451.871 kb on + strand, within EX31_RS23430 at 451.920 kb on + strand, within EX31_RS23430 at 452.046 kb on + strand, within EX31_RS23430 at 452.123 kb on + strand, within EX31_RS23430 at 452.123 kb on + strand, within EX31_RS23430
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 449,764 + EX31_RS23415 0.88 +2.5 449,785 - +0.4 449,888 - -2.0 449,980 + -0.4 449,980 + -0.2 449,980 + -0.0 449,980 + -1.7 449,980 + +1.1 449,980 + -1.5 449,981 - -0.8 449,981 - -0.6 449,981 - -0.5 449,981 - +1.1 449,981 - -1.3 449,981 - +0.5 449,989 + -0.3 449,990 - -1.0 449,992 + +0.0 449,993 - +0.4 449,993 - +1.2 450,005 - -0.5 450,005 - +2.0 450,033 + -0.8 450,033 + +1.0 450,034 - +0.2 450,116 + -0.4 450,117 - -0.9 450,135 - +1.1 450,147 + -0.7 450,274 + -0.0 450,274 + +0.3 450,275 - +1.0 450,275 - -0.3 450,296 + +1.7 450,297 - -0.5 450,297 - -0.6 450,297 - +0.6 450,297 - +1.0 450,297 - -0.4 450,304 + -0.3 450,304 + -0.0 450,305 - -0.0 450,382 + +0.8 450,382 + +0.1 450,383 - +0.2 450,383 - +1.3 450,383 - -0.0 450,383 - +0.3 450,383 - -0.3 450,451 + -0.6 450,451 + +0.8 450,453 + -0.6 450,459 + -1.2 450,459 + +1.1 450,484 + -1.3 450,485 - -0.2 450,535 + -0.7 450,562 + +0.4 450,609 + +0.9 450,609 + -1.5 450,609 + +0.1 450,702 + +1.6 450,702 + -0.2 450,702 + -1.0 450,702 + +0.4 450,702 + -0.2 450,702 + -1.1 450,703 - -0.6 450,824 + EX31_RS23420 0.22 +0.7 450,825 - EX31_RS23420 0.22 -1.5 450,846 + EX31_RS23420 0.27 -2.6 450,846 + EX31_RS23420 0.27 -0.3 450,846 + EX31_RS23420 0.27 +0.3 450,846 + EX31_RS23420 0.27 -0.0 450,846 + EX31_RS23420 0.27 -0.1 450,846 + EX31_RS23420 0.27 +1.1 450,936 + EX31_RS23420 0.51 -1.7 450,936 + EX31_RS23420 0.51 -0.8 450,936 + EX31_RS23420 0.51 -2.0 450,985 + EX31_RS23420 0.63 +0.4 451,004 + EX31_RS23420 0.68 -0.0 451,126 + +2.0 451,151 + -0.4 451,206 + EX31_RS23425 0.24 -0.4 451,206 + EX31_RS23425 0.24 -0.6 451,206 + EX31_RS23425 0.24 +0.1 451,206 + EX31_RS23425 0.24 +1.2 451,206 + EX31_RS23425 0.24 -0.1 451,206 + EX31_RS23425 0.24 -2.7 451,206 + EX31_RS23425 0.24 -0.8 451,206 + EX31_RS23425 0.24 -0.5 451,206 + EX31_RS23425 0.24 -0.5 451,208 + EX31_RS23425 0.25 -0.8 451,208 + EX31_RS23425 0.25 +0.0 451,209 - EX31_RS23425 0.25 -1.5 451,217 + EX31_RS23425 0.27 -0.2 451,217 + EX31_RS23425 0.27 -2.3 451,217 + EX31_RS23425 0.27 -0.8 451,297 + EX31_RS23425 0.50 -0.5 451,297 + EX31_RS23425 0.50 -1.1 451,368 + EX31_RS23425 0.71 -0.8 451,431 + EX31_RS23425 0.89 -1.6 451,431 + EX31_RS23425 0.89 -2.7 451,435 + -0.5 451,435 + +0.2 451,440 + +0.0 451,467 + +0.1 451,467 + -0.6 451,467 + -0.4 451,467 + -0.7 451,607 + +0.4 451,680 + EX31_RS23430 0.12 -1.5 451,680 + EX31_RS23430 0.12 +0.2 451,680 + EX31_RS23430 0.12 -0.7 451,680 + EX31_RS23430 0.12 -0.4 451,680 + EX31_RS23430 0.12 -0.9 451,704 + EX31_RS23430 0.13 +2.0 451,704 + EX31_RS23430 0.13 -1.7 451,704 + EX31_RS23430 0.13 +1.2 451,704 + EX31_RS23430 0.13 +0.4 451,704 + EX31_RS23430 0.13 -2.3 451,705 - EX31_RS23430 0.13 +0.6 451,706 + EX31_RS23430 0.13 -0.6 451,731 + EX31_RS23430 0.15 +0.1 451,731 + EX31_RS23430 0.15 -1.0 451,731 + EX31_RS23430 0.15 +1.2 451,731 + EX31_RS23430 0.15 -1.8 451,731 + EX31_RS23430 0.15 -1.5 451,731 + EX31_RS23430 0.15 +1.4 451,731 + EX31_RS23430 0.15 +0.5 451,732 - EX31_RS23430 0.15 -1.7 451,732 - EX31_RS23430 0.15 +2.0 451,732 - EX31_RS23430 0.15 +0.3 451,733 + EX31_RS23430 0.15 +0.2 451,733 + EX31_RS23430 0.15 +0.7 451,733 + EX31_RS23430 0.15 -2.8 451,733 + EX31_RS23430 0.15 +1.8 451,733 + EX31_RS23430 0.15 -0.2 451,733 + EX31_RS23430 0.15 -2.2 451,733 + EX31_RS23430 0.15 -0.8 451,733 + EX31_RS23430 0.15 -0.6 451,815 + EX31_RS23430 0.19 -1.8 451,815 + EX31_RS23430 0.19 -0.6 451,816 - EX31_RS23430 0.19 +0.5 451,871 + EX31_RS23430 0.23 +0.8 451,920 + EX31_RS23430 0.25 -0.3 452,046 + EX31_RS23430 0.32 +0.7 452,123 + EX31_RS23430 0.37 -2.2 452,123 + EX31_RS23430 0.37 -1.6
Or see this region's nucleotide sequence