Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22990 and EX31_RS22995 are separated by 28 nucleotides EX31_RS22995 and EX31_RS23000 are separated by 33 nucleotides EX31_RS23000 and EX31_RS23005 are separated by 39 nucleotides EX31_RS23005 and EX31_RS23010 are separated by 311 nucleotides
EX31_RS22990: EX31_RS22990 - diguanylate cyclase, at 360,817 to 361,893
_RS22990
EX31_RS22995: EX31_RS22995 - hypothetical protein, at 361,922 to 362,275
_RS22995
EX31_RS23000: EX31_RS23000 - cupin domain-containing protein, at 362,309 to 362,830
_RS23000
EX31_RS23005: EX31_RS23005 - hypothetical protein, at 362,870 to 363,115
_RS23005
EX31_RS23010: EX31_RS23010 - phosphatase, at 363,427 to 364,440
_RS23010
Position (kb) 362
363
364 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 361.872 kb on + strand at 361.872 kb on + strand at 361.872 kb on + strand at 361.872 kb on + strand at 361.872 kb on + strand at 361.872 kb on + strand at 361.872 kb on + strand at 361.872 kb on + strand at 361.873 kb on - strand at 361.873 kb on - strand at 361.895 kb on + strand at 361.895 kb on + strand at 361.896 kb on - strand at 361.901 kb on + strand at 361.920 kb on + strand at 361.931 kb on - strand at 361.938 kb on + strand at 361.938 kb on + strand at 361.938 kb on + strand at 361.938 kb on + strand at 361.939 kb on - strand at 361.939 kb on - strand at 361.939 kb on - strand at 361.939 kb on - strand at 361.939 kb on - strand at 361.990 kb on + strand, within EX31_RS22995 at 361.991 kb on - strand, within EX31_RS22995 at 362.018 kb on + strand, within EX31_RS22995 at 362.018 kb on + strand, within EX31_RS22995 at 362.019 kb on - strand, within EX31_RS22995 at 362.066 kb on + strand, within EX31_RS22995 at 362.066 kb on + strand, within EX31_RS22995 at 362.067 kb on - strand, within EX31_RS22995 at 362.067 kb on - strand, within EX31_RS22995 at 362.067 kb on - strand, within EX31_RS22995 at 362.069 kb on - strand, within EX31_RS22995 at 362.117 kb on + strand, within EX31_RS22995 at 362.117 kb on + strand, within EX31_RS22995 at 362.187 kb on + strand, within EX31_RS22995 at 362.193 kb on + strand, within EX31_RS22995 at 362.193 kb on + strand, within EX31_RS22995 at 362.193 kb on + strand, within EX31_RS22995 at 362.193 kb on + strand, within EX31_RS22995 at 362.193 kb on + strand, within EX31_RS22995 at 362.193 kb on + strand, within EX31_RS22995 at 362.193 kb on + strand, within EX31_RS22995 at 362.194 kb on - strand, within EX31_RS22995 at 362.194 kb on - strand, within EX31_RS22995 at 362.194 kb on - strand, within EX31_RS22995 at 362.247 kb on + strand at 362.273 kb on + strand at 362.273 kb on + strand at 362.274 kb on - strand at 362.309 kb on - strand at 362.309 kb on - strand at 362.375 kb on + strand, within EX31_RS23000 at 362.446 kb on + strand, within EX31_RS23000 at 362.446 kb on + strand, within EX31_RS23000 at 362.463 kb on + strand, within EX31_RS23000 at 362.463 kb on + strand, within EX31_RS23000 at 362.463 kb on + strand, within EX31_RS23000 at 362.463 kb on + strand, within EX31_RS23000 at 362.463 kb on + strand, within EX31_RS23000 at 362.464 kb on - strand, within EX31_RS23000 at 362.464 kb on - strand, within EX31_RS23000 at 362.632 kb on + strand, within EX31_RS23000 at 362.632 kb on + strand, within EX31_RS23000 at 362.632 kb on + strand, within EX31_RS23000 at 362.632 kb on + strand, within EX31_RS23000 at 362.680 kb on + strand, within EX31_RS23000 at 362.680 kb on + strand, within EX31_RS23000 at 362.699 kb on + strand, within EX31_RS23000 at 362.700 kb on - strand, within EX31_RS23000 at 362.720 kb on + strand, within EX31_RS23000 at 362.720 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.754 kb on + strand, within EX31_RS23000 at 362.755 kb on - strand, within EX31_RS23000 at 362.755 kb on - strand, within EX31_RS23000 at 362.861 kb on + strand at 362.871 kb on + strand at 362.872 kb on - strand at 362.872 kb on - strand at 362.961 kb on + strand, within EX31_RS23005 at 362.961 kb on + strand, within EX31_RS23005 at 362.975 kb on - strand, within EX31_RS23005 at 362.982 kb on - strand, within EX31_RS23005 at 362.982 kb on - strand, within EX31_RS23005 at 363.005 kb on - strand, within EX31_RS23005 at 363.043 kb on + strand, within EX31_RS23005 at 363.056 kb on - strand, within EX31_RS23005 at 363.061 kb on + strand, within EX31_RS23005 at 363.061 kb on + strand, within EX31_RS23005 at 363.061 kb on + strand, within EX31_RS23005 at 363.061 kb on + strand, within EX31_RS23005 at 363.061 kb on + strand, within EX31_RS23005 at 363.061 kb on + strand, within EX31_RS23005 at 363.062 kb on - strand, within EX31_RS23005 at 363.062 kb on - strand, within EX31_RS23005 at 363.062 kb on - strand, within EX31_RS23005 at 363.062 kb on - strand, within EX31_RS23005 at 363.062 kb on - strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.063 kb on + strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.064 kb on - strand, within EX31_RS23005 at 363.115 kb on + strand at 363.115 kb on + strand at 363.116 kb on - strand at 363.116 kb on - strand at 363.116 kb on - strand at 363.116 kb on - strand at 363.162 kb on + strand at 363.162 kb on + strand at 363.163 kb on - strand at 363.163 kb on - strand at 363.163 kb on - strand at 363.199 kb on - strand at 363.212 kb on + strand at 363.212 kb on + strand at 363.213 kb on - strand at 363.225 kb on + strand at 363.225 kb on + strand at 363.294 kb on + strand at 363.323 kb on + strand at 363.334 kb on + strand at 363.335 kb on - strand at 363.345 kb on + strand at 363.346 kb on - strand at 363.368 kb on + strand at 363.405 kb on + strand at 363.461 kb on + strand at 363.461 kb on + strand at 363.461 kb on + strand at 363.567 kb on + strand, within EX31_RS23010 at 363.567 kb on + strand, within EX31_RS23010 at 363.567 kb on + strand, within EX31_RS23010 at 363.567 kb on + strand, within EX31_RS23010 at 363.598 kb on + strand, within EX31_RS23010 at 363.598 kb on + strand, within EX31_RS23010 at 363.598 kb on + strand, within EX31_RS23010 at 363.993 kb on + strand, within EX31_RS23010 at 363.993 kb on + strand, within EX31_RS23010 at 363.994 kb on - strand, within EX31_RS23010 at 363.994 kb on - strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.995 kb on + strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.996 kb on - strand, within EX31_RS23010 at 363.997 kb on + strand, within EX31_RS23010 at 363.997 kb on + strand, within EX31_RS23010 at 363.997 kb on + strand, within EX31_RS23010 at 363.997 kb on + strand, within EX31_RS23010 at 363.997 kb on + strand, within EX31_RS23010 at 363.997 kb on + strand, within EX31_RS23010 at 363.998 kb on - strand, within EX31_RS23010 at 363.998 kb on - strand, within EX31_RS23010 at 363.998 kb on - strand, within EX31_RS23010 at 363.998 kb on - strand, within EX31_RS23010 at 363.998 kb on - strand, within EX31_RS23010 at 364.038 kb on + strand, within EX31_RS23010 at 364.050 kb on + strand, within EX31_RS23010 at 364.107 kb on + strand, within EX31_RS23010 at 364.107 kb on + strand, within EX31_RS23010 at 364.108 kb on - strand, within EX31_RS23010
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 361,872 + +0.8 361,872 + -0.5 361,872 + +0.0 361,872 + +0.2 361,872 + +0.1 361,872 + +1.0 361,872 + -0.4 361,872 + -1.6 361,873 - +0.8 361,873 - +0.1 361,895 + +0.9 361,895 + +1.1 361,896 - -0.3 361,901 + -0.1 361,920 + -0.8 361,931 - +1.0 361,938 + -0.6 361,938 + +0.5 361,938 + +1.6 361,938 + -0.2 361,939 - +0.4 361,939 - -0.5 361,939 - +0.7 361,939 - -0.3 361,939 - -0.5 361,990 + EX31_RS22995 0.19 +0.4 361,991 - EX31_RS22995 0.19 -1.3 362,018 + EX31_RS22995 0.27 +0.0 362,018 + EX31_RS22995 0.27 +0.0 362,019 - EX31_RS22995 0.27 +1.0 362,066 + EX31_RS22995 0.41 +0.2 362,066 + EX31_RS22995 0.41 +1.4 362,067 - EX31_RS22995 0.41 +0.6 362,067 - EX31_RS22995 0.41 -1.8 362,067 - EX31_RS22995 0.41 +1.1 362,069 - EX31_RS22995 0.42 -0.7 362,117 + EX31_RS22995 0.55 +0.8 362,117 + EX31_RS22995 0.55 +0.6 362,187 + EX31_RS22995 0.75 +0.6 362,193 + EX31_RS22995 0.77 +0.8 362,193 + EX31_RS22995 0.77 +0.6 362,193 + EX31_RS22995 0.77 -0.1 362,193 + EX31_RS22995 0.77 +0.8 362,193 + EX31_RS22995 0.77 +0.4 362,193 + EX31_RS22995 0.77 -0.2 362,193 + EX31_RS22995 0.77 +0.3 362,194 - EX31_RS22995 0.77 +0.7 362,194 - EX31_RS22995 0.77 -0.5 362,194 - EX31_RS22995 0.77 -0.7 362,247 + +0.4 362,273 + +0.5 362,273 + -1.2 362,274 - +1.0 362,309 - +1.4 362,309 - +1.0 362,375 + EX31_RS23000 0.13 -0.1 362,446 + EX31_RS23000 0.26 +0.1 362,446 + EX31_RS23000 0.26 -0.1 362,463 + EX31_RS23000 0.30 +0.1 362,463 + EX31_RS23000 0.30 -0.3 362,463 + EX31_RS23000 0.30 +0.3 362,463 + EX31_RS23000 0.30 +0.2 362,463 + EX31_RS23000 0.30 +1.5 362,464 - EX31_RS23000 0.30 +0.0 362,464 - EX31_RS23000 0.30 +0.9 362,632 + EX31_RS23000 0.62 -0.9 362,632 + EX31_RS23000 0.62 -1.4 362,632 + EX31_RS23000 0.62 +1.6 362,632 + EX31_RS23000 0.62 +0.4 362,680 + EX31_RS23000 0.71 -2.1 362,680 + EX31_RS23000 0.71 -1.0 362,699 + EX31_RS23000 0.75 +1.5 362,700 - EX31_RS23000 0.75 -1.5 362,720 + EX31_RS23000 0.79 -0.1 362,720 + EX31_RS23000 0.79 -1.0 362,754 + EX31_RS23000 0.85 -0.2 362,754 + EX31_RS23000 0.85 -0.3 362,754 + EX31_RS23000 0.85 +1.0 362,754 + EX31_RS23000 0.85 -0.1 362,754 + EX31_RS23000 0.85 +0.6 362,754 + EX31_RS23000 0.85 +0.4 362,754 + EX31_RS23000 0.85 -0.4 362,754 + EX31_RS23000 0.85 +0.4 362,755 - EX31_RS23000 0.85 -2.1 362,755 - EX31_RS23000 0.85 +0.0 362,861 + -0.3 362,871 + -1.0 362,872 - +0.2 362,872 - -0.5 362,961 + EX31_RS23005 0.37 +1.0 362,961 + EX31_RS23005 0.37 +2.0 362,975 - EX31_RS23005 0.43 +0.4 362,982 - EX31_RS23005 0.46 +1.0 362,982 - EX31_RS23005 0.46 +1.6 363,005 - EX31_RS23005 0.55 +0.9 363,043 + EX31_RS23005 0.70 +0.3 363,056 - EX31_RS23005 0.76 +0.1 363,061 + EX31_RS23005 0.78 +0.5 363,061 + EX31_RS23005 0.78 +0.7 363,061 + EX31_RS23005 0.78 -0.2 363,061 + EX31_RS23005 0.78 +1.6 363,061 + EX31_RS23005 0.78 +0.3 363,061 + EX31_RS23005 0.78 -0.9 363,062 - EX31_RS23005 0.78 +1.3 363,062 - EX31_RS23005 0.78 +0.8 363,062 - EX31_RS23005 0.78 +0.3 363,062 - EX31_RS23005 0.78 -0.6 363,062 - EX31_RS23005 0.78 -1.5 363,063 + EX31_RS23005 0.78 +0.4 363,063 + EX31_RS23005 0.78 +0.4 363,063 + EX31_RS23005 0.78 +1.6 363,063 + EX31_RS23005 0.78 +1.0 363,063 + EX31_RS23005 0.78 -1.6 363,063 + EX31_RS23005 0.78 -0.5 363,063 + EX31_RS23005 0.78 -0.9 363,063 + EX31_RS23005 0.78 +1.2 363,064 - EX31_RS23005 0.79 -0.3 363,064 - EX31_RS23005 0.79 +0.2 363,064 - EX31_RS23005 0.79 -0.7 363,064 - EX31_RS23005 0.79 +0.9 363,064 - EX31_RS23005 0.79 -0.3 363,064 - EX31_RS23005 0.79 +0.9 363,064 - EX31_RS23005 0.79 +0.4 363,064 - EX31_RS23005 0.79 +1.0 363,115 + +0.4 363,115 + +0.7 363,116 - +1.0 363,116 - +0.4 363,116 - -0.2 363,116 - +0.6 363,162 + -0.2 363,162 + -1.8 363,163 - +0.0 363,163 - -0.6 363,163 - -0.2 363,199 - +0.1 363,212 + -0.6 363,212 + -1.1 363,213 - -0.7 363,225 + -1.3 363,225 + +0.6 363,294 + +0.9 363,323 + -1.4 363,334 + +0.1 363,335 - +0.4 363,345 + +0.6 363,346 - +0.8 363,368 + +1.1 363,405 + +0.1 363,461 + +0.9 363,461 + -1.0 363,461 + -0.7 363,567 + EX31_RS23010 0.14 +0.1 363,567 + EX31_RS23010 0.14 -0.1 363,567 + EX31_RS23010 0.14 +0.8 363,567 + EX31_RS23010 0.14 +0.3 363,598 + EX31_RS23010 0.17 -0.4 363,598 + EX31_RS23010 0.17 +0.8 363,598 + EX31_RS23010 0.17 -0.8 363,993 + EX31_RS23010 0.56 -0.1 363,993 + EX31_RS23010 0.56 +0.7 363,994 - EX31_RS23010 0.56 +1.0 363,994 - EX31_RS23010 0.56 -0.8 363,995 + EX31_RS23010 0.56 +0.3 363,995 + EX31_RS23010 0.56 -0.9 363,995 + EX31_RS23010 0.56 +0.2 363,995 + EX31_RS23010 0.56 +0.3 363,995 + EX31_RS23010 0.56 +0.1 363,995 + EX31_RS23010 0.56 -0.5 363,995 + EX31_RS23010 0.56 +0.5 363,995 + EX31_RS23010 0.56 +0.4 363,995 + EX31_RS23010 0.56 +0.6 363,995 + EX31_RS23010 0.56 -0.2 363,995 + EX31_RS23010 0.56 -1.1 363,995 + EX31_RS23010 0.56 -0.4 363,995 + EX31_RS23010 0.56 +0.8 363,995 + EX31_RS23010 0.56 +1.1 363,995 + EX31_RS23010 0.56 +1.3 363,995 + EX31_RS23010 0.56 -0.2 363,995 + EX31_RS23010 0.56 -0.4 363,995 + EX31_RS23010 0.56 +1.2 363,995 + EX31_RS23010 0.56 -0.6 363,995 + EX31_RS23010 0.56 +0.7 363,995 + EX31_RS23010 0.56 +1.0 363,995 + EX31_RS23010 0.56 +0.8 363,995 + EX31_RS23010 0.56 +1.8 363,995 + EX31_RS23010 0.56 +0.6 363,996 - EX31_RS23010 0.56 +0.1 363,996 - EX31_RS23010 0.56 +1.3 363,996 - EX31_RS23010 0.56 +0.4 363,996 - EX31_RS23010 0.56 +0.3 363,996 - EX31_RS23010 0.56 +1.9 363,996 - EX31_RS23010 0.56 +0.7 363,996 - EX31_RS23010 0.56 +0.8 363,996 - EX31_RS23010 0.56 +0.8 363,996 - EX31_RS23010 0.56 +1.1 363,996 - EX31_RS23010 0.56 -0.2 363,996 - EX31_RS23010 0.56 +0.4 363,996 - EX31_RS23010 0.56 +1.1 363,996 - EX31_RS23010 0.56 -1.3 363,996 - EX31_RS23010 0.56 -0.0 363,996 - EX31_RS23010 0.56 +0.8 363,996 - EX31_RS23010 0.56 +0.7 363,996 - EX31_RS23010 0.56 +0.5 363,996 - EX31_RS23010 0.56 +1.3 363,996 - EX31_RS23010 0.56 -0.9 363,996 - EX31_RS23010 0.56 +0.2 363,996 - EX31_RS23010 0.56 -0.8 363,996 - EX31_RS23010 0.56 -0.2 363,996 - EX31_RS23010 0.56 +1.8 363,996 - EX31_RS23010 0.56 +0.8 363,996 - EX31_RS23010 0.56 +0.2 363,996 - EX31_RS23010 0.56 -0.8 363,996 - EX31_RS23010 0.56 -1.4 363,996 - EX31_RS23010 0.56 -0.4 363,996 - EX31_RS23010 0.56 -0.8 363,997 + EX31_RS23010 0.56 -0.9 363,997 + EX31_RS23010 0.56 +1.4 363,997 + EX31_RS23010 0.56 -0.8 363,997 + EX31_RS23010 0.56 -2.4 363,997 + EX31_RS23010 0.56 -1.5 363,997 + EX31_RS23010 0.56 +0.5 363,998 - EX31_RS23010 0.56 +0.5 363,998 - EX31_RS23010 0.56 -0.0 363,998 - EX31_RS23010 0.56 +0.1 363,998 - EX31_RS23010 0.56 +0.7 363,998 - EX31_RS23010 0.56 +0.5 364,038 + EX31_RS23010 0.60 +0.3 364,050 + EX31_RS23010 0.61 -0.7 364,107 + EX31_RS23010 0.67 +0.7 364,107 + EX31_RS23010 0.67 +3.0 364,108 - EX31_RS23010 0.67 +0.3
Or see this region's nucleotide sequence