Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22360 and EX31_RS22365 are separated by 820 nucleotides
EX31_RS22360: EX31_RS22360 - hypothetical protein, at 224,781 to 225,152
_RS22360
EX31_RS22365: EX31_RS22365 - hypothetical protein, at 225,973 to 226,401
_RS22365
Position (kb) 224
225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 223.845 kb on + strand at 223.883 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.906 kb on + strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.907 kb on - strand at 223.954 kb on + strand at 223.954 kb on + strand at 223.954 kb on + strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.955 kb on - strand at 223.976 kb on + strand at 223.976 kb on + strand at 223.976 kb on + strand at 223.977 kb on - strand at 223.977 kb on - strand at 224.052 kb on + strand at 224.089 kb on + strand at 224.121 kb on + strand at 224.121 kb on + strand at 224.121 kb on + strand at 224.139 kb on - strand at 224.147 kb on - strand at 224.363 kb on - strand at 224.405 kb on + strand at 224.406 kb on - strand at 224.494 kb on - strand at 224.581 kb on + strand at 224.582 kb on - strand at 224.659 kb on + strand at 224.660 kb on - strand at 224.671 kb on + strand at 224.672 kb on - strand at 224.672 kb on - strand at 224.672 kb on - strand at 224.672 kb on - strand at 224.672 kb on - strand at 224.676 kb on - strand at 224.676 kb on - strand at 224.676 kb on - strand at 224.751 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.761 kb on + strand at 224.762 kb on - strand at 224.772 kb on - strand at 224.772 kb on - strand at 224.772 kb on - strand at 224.783 kb on - strand at 224.783 kb on - strand at 224.813 kb on + strand at 224.845 kb on - strand, within EX31_RS22360 at 224.845 kb on - strand, within EX31_RS22360 at 224.856 kb on + strand, within EX31_RS22360 at 224.856 kb on + strand, within EX31_RS22360 at 224.857 kb on - strand, within EX31_RS22360 at 224.861 kb on + strand, within EX31_RS22360 at 224.861 kb on + strand, within EX31_RS22360 at 224.861 kb on + strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.862 kb on - strand, within EX31_RS22360 at 224.864 kb on + strand, within EX31_RS22360 at 224.864 kb on + strand, within EX31_RS22360 at 224.865 kb on - strand, within EX31_RS22360 at 224.865 kb on - strand, within EX31_RS22360 at 224.922 kb on + strand, within EX31_RS22360 at 224.922 kb on + strand, within EX31_RS22360 at 224.935 kb on + strand, within EX31_RS22360 at 224.935 kb on + strand, within EX31_RS22360 at 224.935 kb on + strand, within EX31_RS22360 at 224.936 kb on - strand, within EX31_RS22360 at 224.968 kb on + strand, within EX31_RS22360 at 224.969 kb on - strand, within EX31_RS22360 at 224.975 kb on + strand, within EX31_RS22360 at 224.981 kb on + strand, within EX31_RS22360 at 224.981 kb on + strand, within EX31_RS22360 at 224.981 kb on + strand, within EX31_RS22360 at 224.981 kb on + strand, within EX31_RS22360 at 224.981 kb on + strand, within EX31_RS22360 at 224.981 kb on + strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.982 kb on - strand, within EX31_RS22360 at 224.987 kb on + strand, within EX31_RS22360 at 224.988 kb on - strand, within EX31_RS22360 at 225.070 kb on + strand, within EX31_RS22360 at 225.070 kb on + strand, within EX31_RS22360 at 225.070 kb on + strand, within EX31_RS22360 at 225.070 kb on + strand, within EX31_RS22360 at 225.071 kb on - strand, within EX31_RS22360 at 225.079 kb on + strand, within EX31_RS22360 at 225.080 kb on - strand, within EX31_RS22360 at 225.145 kb on - strand at 225.165 kb on + strand at 225.165 kb on + strand at 225.165 kb on + strand at 225.165 kb on + strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.186 kb on - strand at 225.232 kb on + strand at 225.232 kb on + strand at 225.232 kb on + strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.233 kb on - strand at 225.236 kb on + strand at 225.236 kb on + strand at 225.237 kb on - strand at 225.237 kb on - strand at 225.237 kb on - strand at 225.237 kb on - strand at 225.264 kb on + strand at 225.299 kb on - strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.377 kb on + strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.378 kb on - strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.381 kb on + strand at 225.382 kb on - strand at 225.382 kb on - strand at 225.382 kb on - strand at 225.382 kb on - strand at 225.382 kb on - strand at 225.382 kb on - strand at 225.382 kb on - strand at 225.382 kb on - strand at 225.403 kb on + strand at 225.459 kb on + strand at 225.548 kb on - strand at 225.575 kb on - strand at 225.612 kb on + strand at 225.612 kb on + strand at 225.670 kb on - strand at 225.681 kb on + strand at 225.682 kb on - strand at 225.682 kb on - strand at 225.696 kb on + strand at 225.696 kb on + strand at 225.728 kb on + strand at 225.738 kb on + strand at 225.739 kb on - strand at 225.739 kb on - strand at 225.763 kb on + strand at 225.776 kb on + strand at 225.816 kb on + strand at 225.816 kb on + strand at 225.817 kb on - strand at 225.817 kb on - strand at 225.817 kb on - strand at 225.817 kb on - strand at 225.902 kb on + strand at 225.909 kb on + strand at 225.909 kb on + strand at 225.909 kb on + strand at 225.911 kb on + strand at 225.942 kb on + strand at 225.960 kb on + strand at 225.960 kb on + strand at 226.146 kb on + strand, within EX31_RS22365 at 226.146 kb on + strand, within EX31_RS22365 at 226.146 kb on + strand, within EX31_RS22365
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 223,845 + +1.0 223,883 + +0.7 223,906 + -0.3 223,906 + +0.1 223,906 + +0.5 223,906 + -0.1 223,906 + +0.0 223,906 + +0.4 223,906 + +0.0 223,906 + +0.9 223,906 + -0.8 223,906 + -0.4 223,906 + +0.7 223,907 - -1.0 223,907 - -0.1 223,907 - +0.0 223,907 - +2.0 223,907 - +1.2 223,907 - +0.6 223,907 - +1.7 223,907 - +0.1 223,907 - -1.8 223,907 - +0.3 223,907 - +0.4 223,907 - +0.7 223,954 + +0.7 223,954 + +0.6 223,954 + -0.3 223,955 - -2.3 223,955 - -0.9 223,955 - -0.9 223,955 - -0.4 223,955 - +0.0 223,955 - -0.0 223,955 - -0.4 223,955 - -1.7 223,955 - +0.6 223,955 - +0.5 223,976 + +0.2 223,976 + -0.4 223,976 + +0.1 223,977 - +0.6 223,977 - -0.2 224,052 + +0.8 224,089 + -0.3 224,121 + -0.7 224,121 + +0.1 224,121 + -0.3 224,139 - +0.5 224,147 - +1.0 224,363 - -1.3 224,405 + +0.2 224,406 - -0.5 224,494 - -1.0 224,581 + +0.8 224,582 - -0.5 224,659 + +1.4 224,660 - +1.0 224,671 + +0.3 224,672 - +0.2 224,672 - +1.5 224,672 - -1.9 224,672 - +0.4 224,672 - +0.5 224,676 - +0.2 224,676 - -0.4 224,676 - +1.2 224,751 + +1.2 224,761 + -0.7 224,761 + -0.3 224,761 + +1.3 224,761 + -0.7 224,761 + -0.2 224,762 - -0.1 224,772 - -0.2 224,772 - -0.5 224,772 - -1.5 224,783 - +0.7 224,783 - -0.1 224,813 + +0.0 224,845 - EX31_RS22360 0.17 -1.9 224,845 - EX31_RS22360 0.17 -0.3 224,856 + EX31_RS22360 0.20 +1.0 224,856 + EX31_RS22360 0.20 -0.6 224,857 - EX31_RS22360 0.20 -0.7 224,861 + EX31_RS22360 0.22 -0.8 224,861 + EX31_RS22360 0.22 -0.5 224,861 + EX31_RS22360 0.22 +0.2 224,862 - EX31_RS22360 0.22 +0.1 224,862 - EX31_RS22360 0.22 -1.7 224,862 - EX31_RS22360 0.22 -1.4 224,862 - EX31_RS22360 0.22 +0.6 224,862 - EX31_RS22360 0.22 -0.8 224,862 - EX31_RS22360 0.22 -1.4 224,862 - EX31_RS22360 0.22 +0.2 224,862 - EX31_RS22360 0.22 -1.2 224,862 - EX31_RS22360 0.22 -1.1 224,862 - EX31_RS22360 0.22 +0.5 224,864 + EX31_RS22360 0.22 -0.9 224,864 + EX31_RS22360 0.22 +0.4 224,865 - EX31_RS22360 0.23 +1.4 224,865 - EX31_RS22360 0.23 +0.7 224,922 + EX31_RS22360 0.38 +0.0 224,922 + EX31_RS22360 0.38 +1.2 224,935 + EX31_RS22360 0.41 +0.5 224,935 + EX31_RS22360 0.41 +0.8 224,935 + EX31_RS22360 0.41 +0.3 224,936 - EX31_RS22360 0.42 -0.3 224,968 + EX31_RS22360 0.50 +0.8 224,969 - EX31_RS22360 0.51 +0.5 224,975 + EX31_RS22360 0.52 -0.2 224,981 + EX31_RS22360 0.54 +0.7 224,981 + EX31_RS22360 0.54 -0.9 224,981 + EX31_RS22360 0.54 -1.2 224,981 + EX31_RS22360 0.54 -1.5 224,981 + EX31_RS22360 0.54 -1.1 224,981 + EX31_RS22360 0.54 -0.3 224,982 - EX31_RS22360 0.54 -1.8 224,982 - EX31_RS22360 0.54 +0.0 224,982 - EX31_RS22360 0.54 -0.8 224,982 - EX31_RS22360 0.54 -0.2 224,982 - EX31_RS22360 0.54 -0.4 224,982 - EX31_RS22360 0.54 -0.4 224,982 - EX31_RS22360 0.54 -0.8 224,982 - EX31_RS22360 0.54 +2.0 224,982 - EX31_RS22360 0.54 +0.0 224,982 - EX31_RS22360 0.54 -0.4 224,982 - EX31_RS22360 0.54 -1.0 224,987 + EX31_RS22360 0.55 -0.0 224,988 - EX31_RS22360 0.56 +0.2 225,070 + EX31_RS22360 0.78 +1.0 225,070 + EX31_RS22360 0.78 +0.6 225,070 + EX31_RS22360 0.78 -0.7 225,070 + EX31_RS22360 0.78 +1.1 225,071 - EX31_RS22360 0.78 +0.9 225,079 + EX31_RS22360 0.80 -0.5 225,080 - EX31_RS22360 0.80 -0.1 225,145 - +1.5 225,165 + -0.1 225,165 + -2.4 225,165 + -1.3 225,165 + -0.2 225,166 - +0.2 225,166 - -0.4 225,166 - -0.2 225,166 - +0.5 225,166 - +0.4 225,166 - -0.7 225,186 - +1.3 225,232 + +0.5 225,232 + +0.5 225,232 + +0.5 225,233 - -0.3 225,233 - +0.2 225,233 - -0.1 225,233 - +0.9 225,233 - +0.3 225,233 - +0.2 225,233 - +1.5 225,233 - +0.1 225,233 - +0.3 225,236 + +1.2 225,236 + -0.0 225,237 - -0.1 225,237 - +0.5 225,237 - +0.5 225,237 - +1.3 225,264 + -1.5 225,299 - +1.0 225,377 + +0.0 225,377 + -0.2 225,377 + +0.5 225,377 + +0.9 225,377 + +0.6 225,377 + +1.0 225,377 + +0.0 225,377 + -1.9 225,377 + +0.3 225,378 - +1.0 225,378 - +0.7 225,378 - -0.2 225,378 - +0.6 225,378 - -0.3 225,378 - -0.4 225,378 - -0.5 225,378 - -1.7 225,378 - -0.0 225,378 - -1.7 225,381 + -0.7 225,381 + -0.9 225,381 + -0.1 225,381 + +0.3 225,381 + +1.2 225,381 + -0.4 225,381 + -1.7 225,381 + -0.9 225,381 + +0.7 225,381 + +1.2 225,382 - +0.0 225,382 - -0.3 225,382 - -1.1 225,382 - -0.1 225,382 - -0.0 225,382 - +0.9 225,382 - +0.6 225,382 - -1.5 225,403 + -0.3 225,459 + +0.8 225,548 - -0.6 225,575 - -1.4 225,612 + +1.3 225,612 + +0.2 225,670 - +0.5 225,681 + +0.3 225,682 - +0.6 225,682 - -0.5 225,696 + -0.0 225,696 + -0.5 225,728 + -0.3 225,738 + +1.1 225,739 - +0.2 225,739 - -0.6 225,763 + -1.0 225,776 + +0.1 225,816 + -0.8 225,816 + -2.8 225,817 - +0.6 225,817 - +0.6 225,817 - -0.3 225,817 - -0.7 225,902 + -2.8 225,909 + +0.6 225,909 + -0.7 225,909 + -0.2 225,911 + -1.5 225,942 + +0.7 225,960 + -0.4 225,960 + +0.0 226,146 + EX31_RS22365 0.40 -0.3 226,146 + EX31_RS22365 0.40 +0.7 226,146 + EX31_RS22365 0.40 +1.1
Or see this region's nucleotide sequence