Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20440 and EX31_RS20445 are separated by 253 nucleotides EX31_RS20445 and EX31_RS20450 are separated by 104 nucleotides
EX31_RS20440: EX31_RS20440 - diguanylate cyclase, at 720,369 to 721,703
_RS20440
EX31_RS20445: EX31_RS20445 - glutamine-hydrolyzing GMP synthase, at 721,957 to 723,534
_RS20445
EX31_RS20450: EX31_RS20450 - IMP dehydrogenase, at 723,639 to 725,105
_RS20450
Position (kb) 721
722
723
724 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 720.965 kb on + strand, within EX31_RS20440 at 720.965 kb on + strand, within EX31_RS20440 at 720.966 kb on - strand, within EX31_RS20440 at 720.987 kb on - strand, within EX31_RS20440 at 721.107 kb on - strand, within EX31_RS20440 at 721.125 kb on - strand, within EX31_RS20440 at 721.177 kb on + strand, within EX31_RS20440 at 721.187 kb on + strand, within EX31_RS20440 at 721.187 kb on + strand, within EX31_RS20440 at 721.187 kb on + strand, within EX31_RS20440 at 721.187 kb on + strand, within EX31_RS20440 at 721.187 kb on + strand, within EX31_RS20440 at 721.187 kb on + strand, within EX31_RS20440 at 721.187 kb on + strand, within EX31_RS20440 at 721.188 kb on - strand, within EX31_RS20440 at 721.210 kb on + strand, within EX31_RS20440 at 721.211 kb on - strand, within EX31_RS20440 at 721.275 kb on + strand, within EX31_RS20440 at 721.284 kb on + strand, within EX31_RS20440 at 721.316 kb on + strand, within EX31_RS20440 at 721.316 kb on + strand, within EX31_RS20440 at 721.316 kb on + strand, within EX31_RS20440 at 721.322 kb on - strand, within EX31_RS20440 at 721.365 kb on - strand, within EX31_RS20440 at 721.365 kb on - strand, within EX31_RS20440 at 721.431 kb on + strand, within EX31_RS20440 at 721.431 kb on + strand, within EX31_RS20440 at 721.431 kb on + strand, within EX31_RS20440 at 721.431 kb on + strand, within EX31_RS20440 at 721.431 kb on + strand, within EX31_RS20440 at 721.431 kb on + strand, within EX31_RS20440 at 721.431 kb on + strand, within EX31_RS20440 at 721.432 kb on - strand, within EX31_RS20440 at 721.432 kb on - strand, within EX31_RS20440 at 721.432 kb on - strand, within EX31_RS20440 at 721.445 kb on + strand, within EX31_RS20440 at 721.445 kb on + strand, within EX31_RS20440 at 721.445 kb on + strand, within EX31_RS20440 at 721.446 kb on - strand, within EX31_RS20440 at 721.577 kb on + strand at 721.577 kb on + strand at 721.578 kb on - strand at 721.578 kb on - strand at 721.580 kb on - strand at 721.589 kb on + strand at 721.589 kb on + strand at 721.590 kb on - strand at 721.590 kb on - strand at 721.590 kb on - strand at 721.590 kb on - strand at 721.668 kb on - strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.712 kb on + strand at 721.713 kb on - strand at 721.713 kb on - strand at 721.713 kb on - strand at 721.714 kb on + strand at 721.715 kb on - strand at 721.725 kb on + strand at 721.725 kb on + strand at 721.725 kb on + strand at 721.725 kb on + strand at 721.725 kb on + strand at 721.725 kb on + strand at 721.725 kb on + strand at 721.726 kb on - strand at 721.727 kb on + strand at 721.727 kb on + strand at 721.727 kb on + strand at 721.727 kb on + strand at 721.727 kb on + strand at 721.727 kb on + strand at 721.727 kb on + strand at 721.728 kb on - strand at 721.728 kb on - strand at 721.728 kb on - strand at 721.728 kb on - strand at 721.737 kb on + strand at 721.737 kb on + strand at 721.744 kb on - strand at 721.749 kb on + strand at 721.749 kb on + strand at 721.750 kb on - strand at 721.754 kb on + strand at 721.754 kb on + strand at 721.754 kb on + strand at 721.754 kb on + strand at 721.755 kb on - strand at 721.755 kb on - strand at 721.759 kb on - strand at 721.807 kb on - strand at 721.809 kb on + strand at 721.809 kb on + strand at 721.809 kb on + strand at 721.809 kb on + strand at 721.810 kb on - strand at 721.810 kb on - strand at 721.810 kb on - strand at 721.815 kb on + strand at 721.815 kb on + strand at 721.816 kb on - strand at 721.816 kb on - strand at 721.826 kb on - strand at 721.830 kb on + strand at 721.831 kb on - strand at 721.831 kb on - strand at 721.850 kb on - strand at 721.910 kb on + strand at 721.911 kb on - strand at 721.911 kb on - strand at 721.921 kb on - strand at 721.928 kb on + strand at 721.929 kb on - strand at 722.001 kb on - strand at 723.569 kb on - strand at 723.600 kb on - strand at 723.635 kb on - strand at 723.656 kb on - strand at 723.656 kb on - strand at 723.656 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.691 kb on - strand at 723.762 kb on - strand at 723.762 kb on - strand at 723.762 kb on - strand at 723.837 kb on - strand, within EX31_RS20450 at 723.837 kb on - strand, within EX31_RS20450 at 723.839 kb on - strand, within EX31_RS20450 at 723.839 kb on - strand, within EX31_RS20450 at 723.857 kb on + strand, within EX31_RS20450 at 723.902 kb on - strand, within EX31_RS20450 at 723.902 kb on - strand, within EX31_RS20450 at 723.977 kb on - strand, within EX31_RS20450 at 724.055 kb on + strand, within EX31_RS20450 at 724.055 kb on + strand, within EX31_RS20450 at 724.056 kb on - strand, within EX31_RS20450 at 724.188 kb on - strand, within EX31_RS20450 at 724.188 kb on - strand, within EX31_RS20450 at 724.190 kb on + strand, within EX31_RS20450 at 724.190 kb on + strand, within EX31_RS20450 at 724.191 kb on - strand, within EX31_RS20450 at 724.303 kb on + strand, within EX31_RS20450 at 724.304 kb on - strand, within EX31_RS20450 at 724.308 kb on - strand, within EX31_RS20450 at 724.308 kb on - strand, within EX31_RS20450 at 724.308 kb on - strand, within EX31_RS20450 at 724.407 kb on - strand, within EX31_RS20450 at 724.407 kb on - strand, within EX31_RS20450 at 724.473 kb on - strand, within EX31_RS20450 at 724.488 kb on - strand, within EX31_RS20450 at 724.513 kb on - strand, within EX31_RS20450 at 724.513 kb on - strand, within EX31_RS20450 at 724.523 kb on + strand, within EX31_RS20450
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 720,965 + EX31_RS20440 0.45 +0.4 720,965 + EX31_RS20440 0.45 -0.3 720,966 - EX31_RS20440 0.45 +0.5 720,987 - EX31_RS20440 0.46 -0.4 721,107 - EX31_RS20440 0.55 +0.6 721,125 - EX31_RS20440 0.57 +1.3 721,177 + EX31_RS20440 0.61 -1.2 721,187 + EX31_RS20440 0.61 +1.1 721,187 + EX31_RS20440 0.61 +1.5 721,187 + EX31_RS20440 0.61 -0.7 721,187 + EX31_RS20440 0.61 -0.5 721,187 + EX31_RS20440 0.61 +0.2 721,187 + EX31_RS20440 0.61 +0.7 721,187 + EX31_RS20440 0.61 +0.3 721,188 - EX31_RS20440 0.61 +1.8 721,210 + EX31_RS20440 0.63 +0.9 721,211 - EX31_RS20440 0.63 -2.4 721,275 + EX31_RS20440 0.68 +0.1 721,284 + EX31_RS20440 0.69 +1.6 721,316 + EX31_RS20440 0.71 +0.5 721,316 + EX31_RS20440 0.71 -0.9 721,316 + EX31_RS20440 0.71 +0.6 721,322 - EX31_RS20440 0.71 +0.3 721,365 - EX31_RS20440 0.75 -1.0 721,365 - EX31_RS20440 0.75 +1.9 721,431 + EX31_RS20440 0.80 -0.4 721,431 + EX31_RS20440 0.80 -1.0 721,431 + EX31_RS20440 0.80 +0.3 721,431 + EX31_RS20440 0.80 +1.0 721,431 + EX31_RS20440 0.80 +0.9 721,431 + EX31_RS20440 0.80 -0.8 721,431 + EX31_RS20440 0.80 -0.6 721,432 - EX31_RS20440 0.80 +0.8 721,432 - EX31_RS20440 0.80 +1.2 721,432 - EX31_RS20440 0.80 +0.6 721,445 + EX31_RS20440 0.81 +0.2 721,445 + EX31_RS20440 0.81 +0.6 721,445 + EX31_RS20440 0.81 -0.1 721,446 - EX31_RS20440 0.81 +0.5 721,577 + -0.3 721,577 + -0.4 721,578 - +0.7 721,578 - -2.0 721,580 - +0.6 721,589 + -0.4 721,589 + +1.2 721,590 - +1.3 721,590 - +0.6 721,590 - -1.3 721,590 - -0.4 721,668 - -0.1 721,712 + +0.3 721,712 + -0.7 721,712 + -0.7 721,712 + -0.4 721,712 + +0.9 721,712 + +0.1 721,712 + -0.2 721,712 + +0.4 721,712 + +0.8 721,712 + +1.1 721,712 + +0.7 721,713 - +2.0 721,713 - -0.2 721,713 - +0.1 721,714 + -1.3 721,715 - -0.2 721,725 + +0.1 721,725 + +0.0 721,725 + +0.4 721,725 + +0.4 721,725 + +0.7 721,725 + -0.4 721,725 + -0.7 721,726 - +0.7 721,727 + +0.0 721,727 + +0.3 721,727 + +0.9 721,727 + +0.0 721,727 + +1.4 721,727 + +0.7 721,727 + -0.3 721,728 - +0.8 721,728 - +0.9 721,728 - +1.4 721,728 - -0.1 721,737 + +0.6 721,737 + -0.0 721,744 - -0.0 721,749 + +0.5 721,749 + -0.6 721,750 - +0.8 721,754 + -0.1 721,754 + +0.6 721,754 + -0.0 721,754 + -0.1 721,755 - +0.3 721,755 - +2.0 721,759 - -1.1 721,807 - -0.2 721,809 + +1.2 721,809 + +0.6 721,809 + -0.2 721,809 + +1.4 721,810 - -0.5 721,810 - -0.9 721,810 - +0.3 721,815 + -2.1 721,815 + +0.5 721,816 - +0.1 721,816 - -1.0 721,826 - -0.6 721,830 + +0.2 721,831 - +0.6 721,831 - +0.5 721,850 - -0.3 721,910 + +0.4 721,911 - -1.5 721,911 - +0.9 721,921 - -0.9 721,928 + +0.3 721,929 - -0.5 722,001 - -0.6 723,569 - -0.8 723,600 - +0.3 723,635 - -0.5 723,656 - +0.1 723,656 - -0.4 723,656 - -0.4 723,691 - -3.9 723,691 - -0.8 723,691 - -1.1 723,691 - -1.4 723,691 - -3.0 723,691 - -2.7 723,691 - -3.1 723,691 - -1.5 723,691 - -2.6 723,691 - -1.0 723,691 - -2.0 723,691 - -2.7 723,691 - -1.8 723,691 - +0.0 723,691 - -2.6 723,691 - -2.5 723,691 - -1.8 723,762 - -2.0 723,762 - -2.7 723,762 - -2.4 723,837 - EX31_RS20450 0.13 -0.6 723,837 - EX31_RS20450 0.13 +1.2 723,839 - EX31_RS20450 0.14 -2.0 723,839 - EX31_RS20450 0.14 -3.3 723,857 + EX31_RS20450 0.15 -1.4 723,902 - EX31_RS20450 0.18 -1.6 723,902 - EX31_RS20450 0.18 -1.0 723,977 - EX31_RS20450 0.23 -2.0 724,055 + EX31_RS20450 0.28 -0.6 724,055 + EX31_RS20450 0.28 +1.2 724,056 - EX31_RS20450 0.28 -1.4 724,188 - EX31_RS20450 0.37 -2.5 724,188 - EX31_RS20450 0.37 -1.8 724,190 + EX31_RS20450 0.38 -0.4 724,190 + EX31_RS20450 0.38 -2.8 724,191 - EX31_RS20450 0.38 +0.2 724,303 + EX31_RS20450 0.45 -2.2 724,304 - EX31_RS20450 0.45 -0.6 724,308 - EX31_RS20450 0.46 -3.0 724,308 - EX31_RS20450 0.46 -1.5 724,308 - EX31_RS20450 0.46 -2.2 724,407 - EX31_RS20450 0.52 -2.3 724,407 - EX31_RS20450 0.52 -0.6 724,473 - EX31_RS20450 0.57 -1.4 724,488 - EX31_RS20450 0.58 -1.5 724,513 - EX31_RS20450 0.60 -3.3 724,513 - EX31_RS20450 0.60 -1.3 724,523 + EX31_RS20450 0.60 -1.8
Or see this region's nucleotide sequence