Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20405 and EX31_RS20410 overlap by 16 nucleotides EX31_RS20410 and EX31_RS20415 overlap by 17 nucleotides EX31_RS20415 and EX31_RS20420 are separated by 97 nucleotides EX31_RS20420 and EX31_RS20425 are separated by 139 nucleotides
EX31_RS20405: EX31_RS20405 - class I SAM-dependent methyltransferase, at 713,834 to 714,466
_RS20405
EX31_RS20410: EX31_RS20410 - hypothetical protein, at 714,451 to 715,359
_RS20410
EX31_RS20415: EX31_RS20415 - phospholipid carrier-dependent glycosyltransferase, at 715,343 to 717,115
_RS20415
EX31_RS20420: EX31_RS20420 - GNAT family N-acetyltransferase, at 717,213 to 717,647
_RS20420
EX31_RS20425: EX31_RS20425 - DUF3313 domain-containing protein, at 717,787 to 718,446
_RS20425
Position (kb) 715
716
717
718 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 714.358 kb on - strand, within EX31_RS20405 at 714.629 kb on + strand, within EX31_RS20410 at 714.629 kb on + strand, within EX31_RS20410 at 714.630 kb on - strand, within EX31_RS20410 at 714.660 kb on + strand, within EX31_RS20410 at 714.751 kb on + strand, within EX31_RS20410 at 714.792 kb on + strand, within EX31_RS20410 at 714.918 kb on - strand, within EX31_RS20410 at 714.951 kb on - strand, within EX31_RS20410 at 715.111 kb on - strand, within EX31_RS20410 at 715.129 kb on + strand, within EX31_RS20410 at 715.188 kb on - strand, within EX31_RS20410 at 715.309 kb on + strand at 715.506 kb on + strand at 715.566 kb on - strand, within EX31_RS20415 at 715.744 kb on + strand, within EX31_RS20415 at 715.746 kb on + strand, within EX31_RS20415 at 715.751 kb on - strand, within EX31_RS20415 at 715.751 kb on - strand, within EX31_RS20415 at 715.835 kb on + strand, within EX31_RS20415 at 716.019 kb on + strand, within EX31_RS20415 at 716.162 kb on + strand, within EX31_RS20415 at 716.167 kb on + strand, within EX31_RS20415 at 716.167 kb on + strand, within EX31_RS20415 at 716.349 kb on + strand, within EX31_RS20415 at 716.435 kb on - strand, within EX31_RS20415 at 716.552 kb on - strand, within EX31_RS20415 at 716.599 kb on + strand, within EX31_RS20415 at 716.640 kb on + strand, within EX31_RS20415 at 716.644 kb on - strand, within EX31_RS20415 at 716.885 kb on + strand, within EX31_RS20415 at 716.886 kb on - strand, within EX31_RS20415 at 716.886 kb on - strand, within EX31_RS20415 at 716.886 kb on - strand, within EX31_RS20415 at 716.887 kb on + strand, within EX31_RS20415 at 716.911 kb on + strand, within EX31_RS20415 at 716.911 kb on + strand, within EX31_RS20415 at 716.932 kb on + strand, within EX31_RS20415 at 716.933 kb on - strand, within EX31_RS20415 at 716.968 kb on + strand at 716.968 kb on + strand at 716.968 kb on + strand at 716.969 kb on - strand at 716.969 kb on - strand at 716.969 kb on - strand at 716.969 kb on - strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.972 kb on + strand at 716.973 kb on - strand at 716.973 kb on - strand at 716.973 kb on - strand at 716.973 kb on - strand at 716.973 kb on - strand at 716.974 kb on + strand at 716.975 kb on - strand at 716.987 kb on + strand at 717.038 kb on - strand at 717.039 kb on + strand at 717.039 kb on + strand at 717.039 kb on + strand at 717.039 kb on + strand at 717.040 kb on - strand at 717.121 kb on + strand at 717.123 kb on + strand at 717.124 kb on - strand at 717.124 kb on - strand at 717.127 kb on + strand at 717.128 kb on - strand at 717.130 kb on + strand at 717.132 kb on + strand at 717.132 kb on + strand at 717.133 kb on - strand at 717.164 kb on + strand at 717.164 kb on + strand at 717.164 kb on + strand at 717.165 kb on - strand at 717.165 kb on - strand at 717.207 kb on + strand at 717.207 kb on + strand at 717.240 kb on + strand at 717.241 kb on - strand at 717.276 kb on + strand, within EX31_RS20420 at 717.276 kb on + strand, within EX31_RS20420 at 717.276 kb on + strand, within EX31_RS20420 at 717.277 kb on - strand, within EX31_RS20420 at 717.277 kb on - strand, within EX31_RS20420 at 717.296 kb on + strand, within EX31_RS20420 at 717.296 kb on + strand, within EX31_RS20420 at 717.391 kb on + strand, within EX31_RS20420 at 717.392 kb on - strand, within EX31_RS20420 at 717.392 kb on - strand, within EX31_RS20420 at 717.456 kb on + strand, within EX31_RS20420 at 717.538 kb on + strand, within EX31_RS20420 at 717.539 kb on - strand, within EX31_RS20420 at 717.565 kb on - strand, within EX31_RS20420 at 717.618 kb on + strand at 717.618 kb on + strand at 717.618 kb on + strand at 717.621 kb on + strand at 717.621 kb on + strand at 717.622 kb on - strand at 717.622 kb on - strand at 717.622 kb on - strand at 717.664 kb on + strand at 717.679 kb on + strand at 717.679 kb on + strand at 717.746 kb on + strand at 717.746 kb on + strand at 717.746 kb on + strand at 717.746 kb on + strand at 717.747 kb on - strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.749 kb on - strand at 717.749 kb on - strand at 717.749 kb on - strand at 717.749 kb on - strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.767 kb on - strand at 717.797 kb on + strand at 717.798 kb on - strand at 717.890 kb on + strand, within EX31_RS20425 at 717.898 kb on + strand, within EX31_RS20425 at 717.985 kb on + strand, within EX31_RS20425 at 717.985 kb on + strand, within EX31_RS20425 at 717.986 kb on - strand, within EX31_RS20425
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 714,358 - EX31_RS20405 0.83 +0.2 714,629 + EX31_RS20410 0.20 +2.6 714,629 + EX31_RS20410 0.20 +1.2 714,630 - EX31_RS20410 0.20 +0.8 714,660 + EX31_RS20410 0.23 -0.1 714,751 + EX31_RS20410 0.33 -1.7 714,792 + EX31_RS20410 0.38 +1.4 714,918 - EX31_RS20410 0.51 +1.1 714,951 - EX31_RS20410 0.55 -0.5 715,111 - EX31_RS20410 0.73 +0.7 715,129 + EX31_RS20410 0.75 -0.1 715,188 - EX31_RS20410 0.81 +0.0 715,309 + +1.0 715,506 + +1.3 715,566 - EX31_RS20415 0.13 -0.9 715,744 + EX31_RS20415 0.23 +0.7 715,746 + EX31_RS20415 0.23 -0.0 715,751 - EX31_RS20415 0.23 -0.6 715,751 - EX31_RS20415 0.23 +0.0 715,835 + EX31_RS20415 0.28 -1.1 716,019 + EX31_RS20415 0.38 -0.4 716,162 + EX31_RS20415 0.46 +0.6 716,167 + EX31_RS20415 0.46 +0.2 716,167 + EX31_RS20415 0.46 +1.5 716,349 + EX31_RS20415 0.57 +0.2 716,435 - EX31_RS20415 0.62 +0.7 716,552 - EX31_RS20415 0.68 -0.0 716,599 + EX31_RS20415 0.71 -0.2 716,640 + EX31_RS20415 0.73 -0.4 716,644 - EX31_RS20415 0.73 -0.2 716,885 + EX31_RS20415 0.87 +0.4 716,886 - EX31_RS20415 0.87 -1.1 716,886 - EX31_RS20415 0.87 +1.1 716,886 - EX31_RS20415 0.87 -0.1 716,887 + EX31_RS20415 0.87 -1.3 716,911 + EX31_RS20415 0.88 -0.8 716,911 + EX31_RS20415 0.88 -2.6 716,932 + EX31_RS20415 0.90 -1.2 716,933 - EX31_RS20415 0.90 -0.0 716,968 + +0.6 716,968 + -1.3 716,968 + +1.2 716,969 - -1.0 716,969 - -1.1 716,969 - +0.6 716,969 - +0.5 716,972 + +0.8 716,972 + +0.2 716,972 + -0.2 716,972 + +1.1 716,972 + +0.3 716,972 + +0.2 716,972 + -0.2 716,972 + +0.2 716,972 + -1.0 716,973 - -0.8 716,973 - +2.2 716,973 - +0.1 716,973 - +0.7 716,973 - +0.2 716,974 + -0.1 716,975 - +0.7 716,987 + +0.4 717,038 - -0.4 717,039 + -0.6 717,039 + +0.6 717,039 + +0.5 717,039 + -0.2 717,040 - -0.1 717,121 + +0.3 717,123 + -1.5 717,124 - -1.1 717,124 - -0.1 717,127 + -2.1 717,128 - +1.6 717,130 + +0.4 717,132 + +0.6 717,132 + +0.4 717,133 - +2.2 717,164 + -0.3 717,164 + +0.3 717,164 + +0.2 717,165 - -0.1 717,165 - +0.8 717,207 + -0.2 717,207 + +0.3 717,240 + +0.2 717,241 - +0.1 717,276 + EX31_RS20420 0.14 -0.9 717,276 + EX31_RS20420 0.14 -0.1 717,276 + EX31_RS20420 0.14 -0.4 717,277 - EX31_RS20420 0.15 -0.0 717,277 - EX31_RS20420 0.15 -0.4 717,296 + EX31_RS20420 0.19 +0.5 717,296 + EX31_RS20420 0.19 -1.0 717,391 + EX31_RS20420 0.41 -0.2 717,392 - EX31_RS20420 0.41 -1.4 717,392 - EX31_RS20420 0.41 +0.8 717,456 + EX31_RS20420 0.56 +0.2 717,538 + EX31_RS20420 0.75 +0.7 717,539 - EX31_RS20420 0.75 +0.3 717,565 - EX31_RS20420 0.81 +0.1 717,618 + +0.6 717,618 + +0.1 717,618 + +0.2 717,621 + -0.3 717,621 + +0.8 717,622 - -0.5 717,622 - +0.0 717,622 - -1.1 717,664 + -0.5 717,679 + -0.9 717,679 + -0.3 717,746 + -1.1 717,746 + +1.2 717,746 + +0.7 717,746 + +0.4 717,747 - -1.6 717,748 + +0.9 717,748 + +0.7 717,748 + +0.5 717,748 + +0.1 717,748 + -1.7 717,748 + -1.1 717,748 + +0.1 717,748 + -0.8 717,748 + -0.9 717,748 + +0.6 717,748 + -0.1 717,748 + +0.8 717,749 - +0.5 717,749 - +0.6 717,749 - -0.1 717,749 - -1.0 717,752 + +0.1 717,752 + -0.1 717,752 + -0.8 717,752 + +0.0 717,752 + -0.3 717,752 + +0.9 717,752 + +1.1 717,752 + +1.0 717,752 + +0.5 717,752 + -0.3 717,752 + -0.2 717,752 + +1.7 717,752 + +0.6 717,752 + -0.2 717,752 + +0.9 717,752 + +0.3 717,752 + -1.0 717,752 + -0.0 717,752 + -0.3 717,752 + +0.1 717,752 + +1.4 717,752 + -0.7 717,752 + -0.1 717,752 + -0.4 717,752 + +0.6 717,753 - +2.9 717,753 - -0.5 717,753 - +2.3 717,753 - +0.7 717,753 - +0.1 717,753 - -0.4 717,753 - +0.8 717,753 - +0.8 717,767 - +0.6 717,797 + -0.1 717,798 - +1.5 717,890 + EX31_RS20425 0.16 +1.5 717,898 + EX31_RS20425 0.17 +1.2 717,985 + EX31_RS20425 0.30 -2.1 717,985 + EX31_RS20425 0.30 -1.8 717,986 - EX31_RS20425 0.30 -0.5
Or see this region's nucleotide sequence