Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19385 and EX31_RS19390 overlap by 4 nucleotides EX31_RS19390 and EX31_RS19395 overlap by 4 nucleotides EX31_RS19395 and EX31_RS19400 are separated by 12 nucleotides EX31_RS19400 and EX31_RS19405 are separated by 14 nucleotides EX31_RS19405 and EX31_RS19410 overlap by 4 nucleotides
EX31_RS19385: EX31_RS19385 - NADH-quinone oxidoreductase subunit L, at 504,251 to 506,098
_RS19385
EX31_RS19390: EX31_RS19390 - NADH-quinone oxidoreductase subunit NuoK, at 506,095 to 506,397
_RS19390
EX31_RS19395: EX31_RS19395 - NADH-quinone oxidoreductase subunit J, at 506,394 to 506,954
_RS19395
EX31_RS19400: EX31_RS19400 - NADH-quinone oxidoreductase subunit NuoI, at 506,967 to 507,509
_RS19400
EX31_RS19405: EX31_RS19405 - NADH-quinone oxidoreductase subunit NuoH, at 507,524 to 508,501
_RS19405
EX31_RS19410: EX31_RS19410 - NADH-quinone oxidoreductase subunit NuoG, at 508,498 to 511,224
_RS19410
Position (kb) 506
507
508 Strain fitness (log2 ratio) -1
0
1
2
3 at 506.085 kb on + strand at 506.085 kb on + strand at 506.086 kb on - strand at 506.119 kb on - strand at 506.212 kb on - strand, within EX31_RS19390 at 506.213 kb on + strand, within EX31_RS19390 at 506.213 kb on + strand, within EX31_RS19390 at 506.214 kb on - strand, within EX31_RS19390 at 506.214 kb on - strand, within EX31_RS19390 at 506.214 kb on - strand, within EX31_RS19390 at 506.214 kb on - strand, within EX31_RS19390 at 506.214 kb on - strand, within EX31_RS19390 at 506.214 kb on - strand, within EX31_RS19390 at 506.243 kb on + strand, within EX31_RS19390 at 506.244 kb on - strand, within EX31_RS19390 at 506.244 kb on - strand, within EX31_RS19390 at 506.308 kb on - strand, within EX31_RS19390 at 506.308 kb on - strand, within EX31_RS19390 at 506.745 kb on + strand, within EX31_RS19395 at 506.766 kb on - strand, within EX31_RS19395 at 506.781 kb on + strand, within EX31_RS19395 at 506.782 kb on - strand, within EX31_RS19395 at 506.810 kb on - strand, within EX31_RS19395 at 506.863 kb on + strand, within EX31_RS19395 at 506.863 kb on + strand, within EX31_RS19395 at 506.864 kb on - strand, within EX31_RS19395 at 506.937 kb on - strand at 506.938 kb on + strand at 506.939 kb on - strand at 507.068 kb on - strand, within EX31_RS19400 at 507.116 kb on - strand, within EX31_RS19400 at 507.287 kb on + strand, within EX31_RS19400 at 507.287 kb on + strand, within EX31_RS19400 at 507.306 kb on + strand, within EX31_RS19400 at 507.307 kb on - strand, within EX31_RS19400 at 507.321 kb on - strand, within EX31_RS19400 at 507.413 kb on - strand, within EX31_RS19400 at 507.451 kb on - strand, within EX31_RS19400 at 507.493 kb on - strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.513 kb on + strand at 507.514 kb on - strand at 507.514 kb on - strand at 507.514 kb on - strand at 507.525 kb on + strand at 507.526 kb on - strand at 507.568 kb on + strand at 507.569 kb on - strand at 507.569 kb on - strand at 507.573 kb on - strand at 507.573 kb on - strand at 507.573 kb on - strand at 507.777 kb on + strand, within EX31_RS19405 at 507.777 kb on + strand, within EX31_RS19405 at 507.778 kb on - strand, within EX31_RS19405 at 507.778 kb on - strand, within EX31_RS19405 at 507.778 kb on - strand, within EX31_RS19405 at 508.036 kb on - strand, within EX31_RS19405 at 508.090 kb on + strand, within EX31_RS19405 at 508.157 kb on - strand, within EX31_RS19405 at 508.252 kb on + strand, within EX31_RS19405 at 508.252 kb on + strand, within EX31_RS19405 at 508.481 kb on + strand at 508.482 kb on - strand at 508.489 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 506,085 + -0.3 506,085 + -0.3 506,086 - +0.1 506,119 - +0.7 506,212 - EX31_RS19390 0.39 +0.9 506,213 + EX31_RS19390 0.39 +0.5 506,213 + EX31_RS19390 0.39 +1.2 506,214 - EX31_RS19390 0.39 +1.5 506,214 - EX31_RS19390 0.39 +1.1 506,214 - EX31_RS19390 0.39 +0.5 506,214 - EX31_RS19390 0.39 +1.1 506,214 - EX31_RS19390 0.39 +1.3 506,214 - EX31_RS19390 0.39 -0.0 506,243 + EX31_RS19390 0.49 +0.5 506,244 - EX31_RS19390 0.49 +0.5 506,244 - EX31_RS19390 0.49 +0.2 506,308 - EX31_RS19390 0.70 -0.3 506,308 - EX31_RS19390 0.70 +0.6 506,745 + EX31_RS19395 0.63 +1.3 506,766 - EX31_RS19395 0.66 +1.1 506,781 + EX31_RS19395 0.69 +0.1 506,782 - EX31_RS19395 0.69 -0.4 506,810 - EX31_RS19395 0.74 -0.4 506,863 + EX31_RS19395 0.84 +0.3 506,863 + EX31_RS19395 0.84 +1.1 506,864 - EX31_RS19395 0.84 -0.3 506,937 - +3.1 506,938 + +0.5 506,939 - -0.6 507,068 - EX31_RS19400 0.19 -0.5 507,116 - EX31_RS19400 0.27 +0.1 507,287 + EX31_RS19400 0.59 +1.1 507,287 + EX31_RS19400 0.59 +2.7 507,306 + EX31_RS19400 0.62 +0.5 507,307 - EX31_RS19400 0.63 +0.6 507,321 - EX31_RS19400 0.65 -0.6 507,413 - EX31_RS19400 0.82 -1.1 507,451 - EX31_RS19400 0.89 +0.5 507,493 - -0.6 507,513 + +0.1 507,513 + +0.9 507,513 + -0.5 507,513 + -0.5 507,513 + +0.7 507,513 + +2.5 507,514 - +1.8 507,514 - +0.3 507,514 - +0.7 507,525 + +0.5 507,526 - -1.1 507,568 + +3.4 507,569 - +0.1 507,569 - -0.5 507,573 - +0.1 507,573 - -0.6 507,573 - +1.3 507,777 + EX31_RS19405 0.26 +0.7 507,777 + EX31_RS19405 0.26 +0.7 507,778 - EX31_RS19405 0.26 +0.1 507,778 - EX31_RS19405 0.26 +1.0 507,778 - EX31_RS19405 0.26 +1.4 508,036 - EX31_RS19405 0.52 -1.4 508,090 + EX31_RS19405 0.58 +0.3 508,157 - EX31_RS19405 0.65 +1.0 508,252 + EX31_RS19405 0.74 +0.8 508,252 + EX31_RS19405 0.74 +2.1 508,481 + +0.5 508,482 - +0.5 508,489 + -0.9
Or see this region's nucleotide sequence