Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10355 and EX31_RS10360 are separated by 193 nucleotides EX31_RS10360 and EX31_RS10365 are separated by 55 nucleotides
EX31_RS10355: EX31_RS10355 - NUDIX domain-containing protein, at 284,442 to 284,924
_RS10355
EX31_RS10360: EX31_RS10360 - ClbS/DfsB family four-helix bundle protein, at 285,118 to 285,636
_RS10360
EX31_RS10365: EX31_RS10365 - hypothetical protein, at 285,692 to 285,910
_RS10365
Position (kb) 284
285 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 283.451 kb on - strand at 283.505 kb on - strand at 283.523 kb on - strand at 283.528 kb on + strand at 283.528 kb on + strand at 283.528 kb on + strand at 283.529 kb on - strand at 283.529 kb on - strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.530 kb on + strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.531 kb on - strand at 283.584 kb on - strand at 283.609 kb on + strand at 283.609 kb on + strand at 283.624 kb on + strand at 283.624 kb on + strand at 283.625 kb on - strand at 283.625 kb on - strand at 283.625 kb on - strand at 283.628 kb on + strand at 283.681 kb on - strand at 283.698 kb on - strand at 283.728 kb on - strand at 283.828 kb on - strand at 283.887 kb on - strand at 283.889 kb on + strand at 283.952 kb on - strand at 283.963 kb on + strand at 284.065 kb on - strand at 284.066 kb on + strand at 284.066 kb on + strand at 284.066 kb on + strand at 284.068 kb on + strand at 284.068 kb on + strand at 284.069 kb on - strand at 284.081 kb on + strand at 284.090 kb on - strand at 284.169 kb on + strand at 284.170 kb on - strand at 284.175 kb on + strand at 284.175 kb on + strand at 284.176 kb on - strand at 284.191 kb on - strand at 284.203 kb on - strand at 284.255 kb on - strand at 284.341 kb on + strand at 284.341 kb on + strand at 284.363 kb on + strand at 284.364 kb on - strand at 284.395 kb on + strand at 284.395 kb on + strand at 284.395 kb on + strand at 284.395 kb on + strand at 284.396 kb on - strand at 284.397 kb on + strand at 284.398 kb on - strand at 284.398 kb on - strand at 284.421 kb on + strand at 284.422 kb on - strand at 284.426 kb on + strand at 284.611 kb on + strand, within EX31_RS10355 at 284.657 kb on - strand, within EX31_RS10355 at 284.729 kb on + strand, within EX31_RS10355 at 284.730 kb on - strand, within EX31_RS10355 at 284.733 kb on - strand, within EX31_RS10355 at 284.747 kb on + strand, within EX31_RS10355 at 284.748 kb on - strand, within EX31_RS10355 at 284.812 kb on + strand, within EX31_RS10355 at 284.813 kb on - strand, within EX31_RS10355 at 284.813 kb on - strand, within EX31_RS10355 at 284.839 kb on + strand, within EX31_RS10355 at 284.869 kb on + strand, within EX31_RS10355 at 284.869 kb on + strand, within EX31_RS10355 at 284.869 kb on + strand, within EX31_RS10355 at 284.869 kb on + strand, within EX31_RS10355 at 284.899 kb on + strand at 284.903 kb on + strand at 284.903 kb on + strand at 284.904 kb on - strand at 284.917 kb on + strand at 284.917 kb on + strand at 285.007 kb on + strand at 285.008 kb on - strand at 285.008 kb on - strand at 285.015 kb on + strand at 285.016 kb on - strand at 285.049 kb on + strand at 285.060 kb on + strand at 285.109 kb on + strand at 285.187 kb on + strand, within EX31_RS10360 at 285.219 kb on - strand, within EX31_RS10360 at 285.219 kb on - strand, within EX31_RS10360 at 285.222 kb on + strand, within EX31_RS10360 at 285.222 kb on + strand, within EX31_RS10360 at 285.437 kb on + strand, within EX31_RS10360 at 285.442 kb on - strand, within EX31_RS10360 at 285.509 kb on - strand, within EX31_RS10360 at 285.509 kb on - strand, within EX31_RS10360 at 285.559 kb on - strand, within EX31_RS10360 at 285.575 kb on - strand, within EX31_RS10360 at 285.619 kb on - strand at 285.619 kb on - strand at 285.621 kb on + strand at 285.622 kb on - strand at 285.629 kb on + strand at 285.629 kb on + strand at 285.662 kb on + strand at 285.690 kb on + strand at 285.691 kb on - strand at 285.701 kb on + strand at 285.701 kb on + strand at 285.861 kb on + strand, within EX31_RS10365 at 285.861 kb on + strand, within EX31_RS10365
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 283,451 - -0.2 283,505 - -2.3 283,523 - -1.4 283,528 + +0.6 283,528 + -0.1 283,528 + +0.7 283,529 - -0.6 283,529 - +0.4 283,530 + -0.1 283,530 + -1.9 283,530 + -0.1 283,530 + +1.1 283,530 + +1.9 283,531 - +1.0 283,531 - +0.8 283,531 - +2.9 283,531 - -0.4 283,531 - +0.3 283,531 - -0.1 283,531 - +0.3 283,531 - +0.5 283,531 - -0.1 283,531 - +1.3 283,531 - +0.2 283,584 - -0.2 283,609 + +0.1 283,609 + +1.5 283,624 + -0.1 283,624 + +0.1 283,625 - +0.1 283,625 - +0.6 283,625 - -0.9 283,628 + +0.1 283,681 - -1.1 283,698 - +1.2 283,728 - +1.3 283,828 - +0.9 283,887 - +0.4 283,889 + +0.5 283,952 - -0.1 283,963 + -0.4 284,065 - +0.6 284,066 + +0.3 284,066 + -0.1 284,066 + +0.3 284,068 + -0.3 284,068 + +1.3 284,069 - +1.2 284,081 + +1.4 284,090 - -0.6 284,169 + -0.6 284,170 - -0.1 284,175 + -1.3 284,175 + +0.3 284,176 - +0.3 284,191 - +1.9 284,203 - -1.2 284,255 - +0.1 284,341 + -1.8 284,341 + +0.3 284,363 + +2.9 284,364 - -0.7 284,395 + +0.3 284,395 + -0.6 284,395 + +0.9 284,395 + +1.1 284,396 - +0.5 284,397 + +1.9 284,398 - +0.3 284,398 - +1.1 284,421 + -0.4 284,422 - -0.1 284,426 + +0.9 284,611 + EX31_RS10355 0.35 -1.2 284,657 - EX31_RS10355 0.45 -0.4 284,729 + EX31_RS10355 0.59 -2.2 284,730 - EX31_RS10355 0.60 -0.6 284,733 - EX31_RS10355 0.60 +1.4 284,747 + EX31_RS10355 0.63 -0.1 284,748 - EX31_RS10355 0.63 +0.4 284,812 + EX31_RS10355 0.77 +0.3 284,813 - EX31_RS10355 0.77 +0.5 284,813 - EX31_RS10355 0.77 +2.9 284,839 + EX31_RS10355 0.82 +0.1 284,869 + EX31_RS10355 0.88 +0.3 284,869 + EX31_RS10355 0.88 -0.2 284,869 + EX31_RS10355 0.88 -0.7 284,869 + EX31_RS10355 0.88 -0.1 284,899 + +0.3 284,903 + -0.1 284,903 + +0.6 284,904 - -0.1 284,917 + +0.3 284,917 + -1.1 285,007 + -0.3 285,008 - -1.3 285,008 - +0.9 285,015 + -0.4 285,016 - +0.4 285,049 + +1.5 285,060 + +0.3 285,109 + -1.0 285,187 + EX31_RS10360 0.13 +2.6 285,219 - EX31_RS10360 0.19 -0.3 285,219 - EX31_RS10360 0.19 +0.6 285,222 + EX31_RS10360 0.20 +1.9 285,222 + EX31_RS10360 0.20 +0.1 285,437 + EX31_RS10360 0.61 -0.2 285,442 - EX31_RS10360 0.62 +1.2 285,509 - EX31_RS10360 0.75 -0.4 285,509 - EX31_RS10360 0.75 +0.6 285,559 - EX31_RS10360 0.85 -2.3 285,575 - EX31_RS10360 0.88 -1.7 285,619 - -0.1 285,619 - -0.4 285,621 + -0.4 285,622 - -0.5 285,629 + +0.2 285,629 + -2.3 285,662 + +1.9 285,690 + +0.3 285,691 - +1.7 285,701 + -0.1 285,701 + +1.6 285,861 + EX31_RS10365 0.77 +1.2 285,861 + EX31_RS10365 0.77 +1.9
Or see this region's nucleotide sequence