Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07940 and EX31_RS07945 are separated by 75 nucleotides EX31_RS07945 and EX31_RS07950 are separated by 348 nucleotides
EX31_RS07940: EX31_RS07940 - phosphopyruvate hydratase, at 71,710 to 73,014
_RS07940
EX31_RS07945: EX31_RS07945 - CTP synthase (glutamine hydrolyzing), at 73,090 to 74,727
_RS07945
EX31_RS07950: EX31_RS07950 - nucleoside triphosphate pyrophosphohydrolase, at 75,076 to 75,879
_RS07950
Position (kb) 73
74
75 Strain fitness (log2 ratio) -2
-1
0
1 at 73.030 kb on - strand at 73.030 kb on - strand at 73.037 kb on - strand at 73.037 kb on - strand at 73.041 kb on - strand at 73.041 kb on - strand at 73.041 kb on - strand at 73.097 kb on - strand at 73.112 kb on + strand at 74.765 kb on - strand at 74.765 kb on - strand at 74.812 kb on + strand at 74.812 kb on + strand at 74.909 kb on - strand at 74.957 kb on - strand at 75.072 kb on + strand at 75.072 kb on + strand at 75.073 kb on - strand at 75.073 kb on - strand at 75.073 kb on - strand at 75.073 kb on - strand at 75.129 kb on + strand at 75.129 kb on + strand at 75.129 kb on + strand at 75.129 kb on + strand at 75.130 kb on - strand at 75.130 kb on - strand at 75.130 kb on - strand at 75.146 kb on - strand at 75.146 kb on - strand at 75.146 kb on - strand at 75.239 kb on - strand, within EX31_RS07950 at 75.239 kb on - strand, within EX31_RS07950 at 75.239 kb on - strand, within EX31_RS07950 at 75.239 kb on - strand, within EX31_RS07950 at 75.354 kb on - strand, within EX31_RS07950 at 75.354 kb on - strand, within EX31_RS07950 at 75.598 kb on + strand, within EX31_RS07950 at 75.598 kb on + strand, within EX31_RS07950 at 75.599 kb on - strand, within EX31_RS07950 at 75.612 kb on + strand, within EX31_RS07950 at 75.624 kb on + strand, within EX31_RS07950 at 75.624 kb on + strand, within EX31_RS07950 at 75.624 kb on + strand, within EX31_RS07950 at 75.624 kb on + strand, within EX31_RS07950 at 75.624 kb on + strand, within EX31_RS07950 at 75.669 kb on - strand, within EX31_RS07950 at 75.669 kb on - strand, within EX31_RS07950 at 75.719 kb on + strand, within EX31_RS07950 at 75.719 kb on + strand, within EX31_RS07950
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 73,030 - -0.2 73,030 - -0.6 73,037 - +0.2 73,037 - +0.1 73,041 - -0.3 73,041 - -0.8 73,041 - -0.3 73,097 - +0.6 73,112 + +0.4 74,765 - -0.9 74,765 - -0.3 74,812 + +0.6 74,812 + -0.4 74,909 - -0.3 74,957 - -0.0 75,072 + -0.5 75,072 + -0.4 75,073 - -0.1 75,073 - -0.8 75,073 - -1.2 75,073 - -0.9 75,129 + +0.5 75,129 + +0.8 75,129 + +1.1 75,129 + -0.6 75,130 - -0.1 75,130 - +0.4 75,130 - +0.7 75,146 - +0.2 75,146 - +0.5 75,146 - +0.2 75,239 - EX31_RS07950 0.20 -1.0 75,239 - EX31_RS07950 0.20 +0.7 75,239 - EX31_RS07950 0.20 -0.1 75,239 - EX31_RS07950 0.20 +0.8 75,354 - EX31_RS07950 0.35 -0.7 75,354 - EX31_RS07950 0.35 -1.7 75,598 + EX31_RS07950 0.65 +0.7 75,598 + EX31_RS07950 0.65 -0.3 75,599 - EX31_RS07950 0.65 -1.0 75,612 + EX31_RS07950 0.67 +1.1 75,624 + EX31_RS07950 0.68 +0.1 75,624 + EX31_RS07950 0.68 -1.9 75,624 + EX31_RS07950 0.68 +0.6 75,624 + EX31_RS07950 0.68 -1.5 75,624 + EX31_RS07950 0.68 -0.4 75,669 - EX31_RS07950 0.74 +1.1 75,669 - EX31_RS07950 0.74 -0.2 75,719 + EX31_RS07950 0.80 +0.8 75,719 + EX31_RS07950 0.80 +0.4
Or see this region's nucleotide sequence