Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07255 and EX31_RS07260 are separated by 209 nucleotides EX31_RS07260 and EX31_RS07265 are separated by 418 nucleotides EX31_RS07265 and EX31_RS07270 are separated by 40 nucleotides
EX31_RS07255: EX31_RS07255 - serine hydrolase, at 250,769 to 252,322
_RS07255
EX31_RS07260: EX31_RS07260 - formate-dependent uric acid utilization protein AegA, at 252,532 to 254,511
_RS07260
EX31_RS07265: EX31_RS07265 - heme-binding protein, at 254,930 to 255,406
_RS07265
EX31_RS07270: EX31_RS07270 - gluconate 5-dehydrogenase, at 255,447 to 256,013
_RS07270
Position (kb)
252
253
254
255 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 251.649 kb on - strand, within EX31_RS07255 at 251.678 kb on - strand, within EX31_RS07255 at 251.678 kb on - strand, within EX31_RS07255 at 251.685 kb on - strand, within EX31_RS07255 at 251.787 kb on + strand, within EX31_RS07255 at 251.787 kb on + strand, within EX31_RS07255 at 251.832 kb on + strand, within EX31_RS07255 at 251.832 kb on + strand, within EX31_RS07255 at 251.832 kb on + strand, within EX31_RS07255 at 251.833 kb on - strand, within EX31_RS07255 at 251.833 kb on - strand, within EX31_RS07255 at 251.913 kb on - strand, within EX31_RS07255 at 252.159 kb on + strand, within EX31_RS07255 at 252.159 kb on + strand, within EX31_RS07255 at 252.243 kb on - strand at 252.433 kb on + strand at 252.442 kb on + strand at 252.442 kb on + strand at 252.476 kb on - strand at 252.478 kb on + strand at 252.479 kb on - strand at 252.512 kb on - strand at 252.544 kb on - strand at 252.544 kb on - strand at 252.790 kb on - strand, within EX31_RS07260 at 252.798 kb on + strand, within EX31_RS07260 at 252.799 kb on - strand, within EX31_RS07260 at 252.843 kb on - strand, within EX31_RS07260 at 252.843 kb on - strand, within EX31_RS07260 at 252.898 kb on - strand, within EX31_RS07260 at 252.898 kb on - strand, within EX31_RS07260 at 253.147 kb on - strand, within EX31_RS07260 at 253.156 kb on + strand, within EX31_RS07260 at 253.157 kb on - strand, within EX31_RS07260 at 253.157 kb on - strand, within EX31_RS07260 at 253.157 kb on - strand, within EX31_RS07260 at 253.157 kb on - strand, within EX31_RS07260 at 253.168 kb on - strand, within EX31_RS07260 at 253.168 kb on - strand, within EX31_RS07260 at 253.168 kb on - strand, within EX31_RS07260 at 253.398 kb on + strand, within EX31_RS07260 at 253.425 kb on + strand, within EX31_RS07260 at 253.435 kb on + strand, within EX31_RS07260 at 253.435 kb on + strand, within EX31_RS07260 at 253.436 kb on - strand, within EX31_RS07260 at 253.436 kb on - strand, within EX31_RS07260 at 253.437 kb on + strand, within EX31_RS07260 at 253.437 kb on + strand, within EX31_RS07260 at 253.438 kb on - strand, within EX31_RS07260 at 253.438 kb on - strand, within EX31_RS07260 at 253.488 kb on + strand, within EX31_RS07260 at 253.498 kb on - strand, within EX31_RS07260 at 253.498 kb on - strand, within EX31_RS07260 at 253.627 kb on - strand, within EX31_RS07260 at 253.766 kb on + strand, within EX31_RS07260 at 253.772 kb on + strand, within EX31_RS07260 at 253.773 kb on - strand, within EX31_RS07260 at 253.773 kb on - strand, within EX31_RS07260 at 253.786 kb on + strand, within EX31_RS07260 at 253.786 kb on + strand, within EX31_RS07260 at 253.786 kb on + strand, within EX31_RS07260 at 253.787 kb on - strand, within EX31_RS07260 at 253.906 kb on + strand, within EX31_RS07260 at 253.907 kb on - strand, within EX31_RS07260 at 253.953 kb on + strand, within EX31_RS07260 at 254.089 kb on - strand, within EX31_RS07260 at 254.089 kb on - strand, within EX31_RS07260 at 254.153 kb on + strand, within EX31_RS07260 at 254.154 kb on - strand, within EX31_RS07260 at 254.277 kb on + strand, within EX31_RS07260 at 254.277 kb on + strand, within EX31_RS07260 at 254.277 kb on + strand, within EX31_RS07260 at 254.278 kb on - strand, within EX31_RS07260 at 254.327 kb on + strand at 254.327 kb on + strand at 254.357 kb on - strand at 254.378 kb on + strand at 254.460 kb on + strand at 254.460 kb on + strand at 254.589 kb on + strand at 254.590 kb on - strand at 254.750 kb on - strand at 255.412 kb on + strand at 255.412 kb on + strand at 255.413 kb on - strand at 255.422 kb on + strand at 255.422 kb on + strand at 255.423 kb on - strand at 255.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 251,649 - EX31_RS07255 0.57 +1.2 251,678 - EX31_RS07255 0.58 -0.8 251,678 - EX31_RS07255 0.58 -0.6 251,685 - EX31_RS07255 0.59 -0.4 251,787 + EX31_RS07255 0.66 -0.1 251,787 + EX31_RS07255 0.66 -0.2 251,832 + EX31_RS07255 0.68 -0.7 251,832 + EX31_RS07255 0.68 -0.4 251,832 + EX31_RS07255 0.68 +1.1 251,833 - EX31_RS07255 0.68 +0.6 251,833 - EX31_RS07255 0.68 +0.3 251,913 - EX31_RS07255 0.74 +4.1 252,159 + EX31_RS07255 0.89 +0.0 252,159 + EX31_RS07255 0.89 -0.2 252,243 - -1.0 252,433 + -0.0 252,442 + +0.2 252,442 + +1.0 252,476 - -0.8 252,478 + +1.2 252,479 - -0.7 252,512 - -0.8 252,544 - +0.4 252,544 - +0.2 252,790 - EX31_RS07260 0.13 +2.8 252,798 + EX31_RS07260 0.13 +1.8 252,799 - EX31_RS07260 0.13 +0.2 252,843 - EX31_RS07260 0.16 -0.6 252,843 - EX31_RS07260 0.16 +1.2 252,898 - EX31_RS07260 0.18 +1.1 252,898 - EX31_RS07260 0.18 +0.8 253,147 - EX31_RS07260 0.31 +0.8 253,156 + EX31_RS07260 0.32 +1.5 253,157 - EX31_RS07260 0.32 -0.8 253,157 - EX31_RS07260 0.32 -0.2 253,157 - EX31_RS07260 0.32 +0.2 253,157 - EX31_RS07260 0.32 -0.1 253,168 - EX31_RS07260 0.32 +0.2 253,168 - EX31_RS07260 0.32 -1.4 253,168 - EX31_RS07260 0.32 +0.8 253,398 + EX31_RS07260 0.44 +1.0 253,425 + EX31_RS07260 0.45 -0.6 253,435 + EX31_RS07260 0.46 -0.4 253,435 + EX31_RS07260 0.46 +1.3 253,436 - EX31_RS07260 0.46 -0.5 253,436 - EX31_RS07260 0.46 +0.4 253,437 + EX31_RS07260 0.46 +1.0 253,437 + EX31_RS07260 0.46 +3.3 253,438 - EX31_RS07260 0.46 +1.0 253,438 - EX31_RS07260 0.46 +2.8 253,488 + EX31_RS07260 0.48 -0.2 253,498 - EX31_RS07260 0.49 -0.6 253,498 - EX31_RS07260 0.49 -1.1 253,627 - EX31_RS07260 0.55 +0.2 253,766 + EX31_RS07260 0.62 -0.8 253,772 + EX31_RS07260 0.63 +0.5 253,773 - EX31_RS07260 0.63 +0.0 253,773 - EX31_RS07260 0.63 +0.5 253,786 + EX31_RS07260 0.63 +1.0 253,786 + EX31_RS07260 0.63 -0.5 253,786 + EX31_RS07260 0.63 -0.9 253,787 - EX31_RS07260 0.63 +0.7 253,906 + EX31_RS07260 0.69 +0.8 253,907 - EX31_RS07260 0.69 -2.0 253,953 + EX31_RS07260 0.72 -0.2 254,089 - EX31_RS07260 0.79 +2.2 254,089 - EX31_RS07260 0.79 -0.7 254,153 + EX31_RS07260 0.82 +0.9 254,154 - EX31_RS07260 0.82 +0.9 254,277 + EX31_RS07260 0.88 +0.5 254,277 + EX31_RS07260 0.88 -0.2 254,277 + EX31_RS07260 0.88 +2.0 254,278 - EX31_RS07260 0.88 +1.2 254,327 + -0.4 254,327 + -0.6 254,357 - -0.0 254,378 + +0.2 254,460 + +0.9 254,460 + +0.9 254,589 + +0.9 254,590 - +0.6 254,750 - +0.3 255,412 + +2.1 255,412 + -0.2 255,413 - -0.6 255,422 + +1.3 255,422 + -0.3 255,423 - +2.3 255,423 - -1.3
Or see this region's nucleotide sequence