Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06030 and EX31_RS06035 are separated by 152 nucleotides EX31_RS06035 and EX31_RS06040 overlap by 11 nucleotides EX31_RS06040 and EX31_RS06045 are separated by 110 nucleotides
EX31_RS06030: EX31_RS06030 - NCS2 family permease, at 366,806 to 368,143
_RS06030
EX31_RS06035: EX31_RS06035 - 6-phosphogluconate phosphatase, at 368,296 to 368,961
_RS06035
EX31_RS06040: EX31_RS06040 - sugar phosphate isomerase/epimerase, at 368,951 to 369,781
_RS06040
EX31_RS06045: EX31_RS06045 - TetR family transcriptional regulator, at 369,892 to 370,584
_RS06045
Position (kb) 368
369 Strain fitness (log2 ratio) -2
-1
0
1
2 at 367.394 kb on - strand, within EX31_RS06030 at 367.467 kb on + strand, within EX31_RS06030 at 367.467 kb on + strand, within EX31_RS06030 at 367.467 kb on + strand, within EX31_RS06030 at 367.467 kb on + strand, within EX31_RS06030 at 367.467 kb on + strand, within EX31_RS06030 at 367.468 kb on - strand, within EX31_RS06030 at 367.468 kb on - strand, within EX31_RS06030 at 367.468 kb on - strand, within EX31_RS06030 at 367.468 kb on - strand, within EX31_RS06030 at 367.468 kb on - strand, within EX31_RS06030 at 367.468 kb on - strand, within EX31_RS06030 at 367.510 kb on + strand, within EX31_RS06030 at 367.511 kb on - strand, within EX31_RS06030 at 367.610 kb on - strand, within EX31_RS06030 at 367.645 kb on + strand, within EX31_RS06030 at 367.645 kb on + strand, within EX31_RS06030 at 367.645 kb on + strand, within EX31_RS06030 at 367.645 kb on + strand, within EX31_RS06030 at 367.645 kb on + strand, within EX31_RS06030 at 367.646 kb on - strand, within EX31_RS06030 at 367.678 kb on + strand, within EX31_RS06030 at 367.679 kb on - strand, within EX31_RS06030 at 367.679 kb on - strand, within EX31_RS06030 at 367.712 kb on + strand, within EX31_RS06030 at 367.712 kb on + strand, within EX31_RS06030 at 367.713 kb on - strand, within EX31_RS06030 at 367.713 kb on - strand, within EX31_RS06030 at 367.713 kb on - strand, within EX31_RS06030 at 367.713 kb on - strand, within EX31_RS06030 at 368.011 kb on + strand at 368.013 kb on + strand at 368.013 kb on + strand at 368.013 kb on + strand at 368.013 kb on + strand at 368.013 kb on + strand at 368.013 kb on + strand at 368.013 kb on + strand at 368.013 kb on + strand at 368.014 kb on - strand at 368.014 kb on - strand at 368.014 kb on - strand at 368.014 kb on - strand at 368.014 kb on - strand at 368.014 kb on - strand at 368.014 kb on - strand at 368.014 kb on - strand at 368.032 kb on + strand at 368.032 kb on + strand at 368.032 kb on + strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.033 kb on - strand at 368.036 kb on + strand at 368.037 kb on - strand at 368.060 kb on + strand at 368.061 kb on - strand at 368.087 kb on - strand at 368.187 kb on - strand at 368.196 kb on - strand at 368.221 kb on + strand at 368.221 kb on + strand at 368.221 kb on + strand at 368.222 kb on - strand at 368.222 kb on - strand at 368.222 kb on - strand at 368.230 kb on - strand at 368.235 kb on + strand at 368.236 kb on - strand at 368.236 kb on - strand at 368.236 kb on - strand at 368.246 kb on + strand at 368.256 kb on + strand at 368.256 kb on + strand at 368.269 kb on + strand at 368.269 kb on + strand at 368.269 kb on + strand at 368.270 kb on - strand at 368.270 kb on - strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.295 kb on + strand at 368.296 kb on - strand at 368.296 kb on - strand at 368.296 kb on - strand at 368.296 kb on - strand at 368.371 kb on + strand, within EX31_RS06035 at 368.371 kb on + strand, within EX31_RS06035 at 368.371 kb on + strand, within EX31_RS06035 at 368.371 kb on + strand, within EX31_RS06035 at 368.371 kb on + strand, within EX31_RS06035 at 368.372 kb on - strand, within EX31_RS06035 at 368.372 kb on - strand, within EX31_RS06035 at 368.372 kb on - strand, within EX31_RS06035 at 368.423 kb on - strand, within EX31_RS06035 at 368.431 kb on + strand, within EX31_RS06035 at 368.431 kb on + strand, within EX31_RS06035 at 368.473 kb on - strand, within EX31_RS06035 at 368.562 kb on + strand, within EX31_RS06035 at 368.562 kb on + strand, within EX31_RS06035 at 368.562 kb on + strand, within EX31_RS06035 at 368.562 kb on + strand, within EX31_RS06035 at 368.562 kb on + strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.563 kb on - strand, within EX31_RS06035 at 368.583 kb on - strand, within EX31_RS06035 at 368.583 kb on - strand, within EX31_RS06035 at 368.666 kb on + strand, within EX31_RS06035 at 368.677 kb on - strand, within EX31_RS06035 at 368.685 kb on + strand, within EX31_RS06035 at 368.685 kb on + strand, within EX31_RS06035 at 368.685 kb on + strand, within EX31_RS06035 at 368.685 kb on + strand, within EX31_RS06035 at 368.740 kb on + strand, within EX31_RS06035 at 368.740 kb on + strand, within EX31_RS06035 at 368.740 kb on + strand, within EX31_RS06035 at 368.740 kb on + strand, within EX31_RS06035 at 368.740 kb on + strand, within EX31_RS06035 at 368.845 kb on + strand, within EX31_RS06035 at 368.846 kb on - strand, within EX31_RS06035 at 368.849 kb on - strand, within EX31_RS06035 at 368.849 kb on - strand, within EX31_RS06035 at 368.865 kb on + strand, within EX31_RS06035 at 369.037 kb on + strand, within EX31_RS06040 at 369.037 kb on + strand, within EX31_RS06040 at 369.037 kb on + strand, within EX31_RS06040 at 369.037 kb on + strand, within EX31_RS06040 at 369.037 kb on + strand, within EX31_RS06040 at 369.038 kb on - strand, within EX31_RS06040 at 369.080 kb on - strand, within EX31_RS06040 at 369.114 kb on - strand, within EX31_RS06040 at 369.141 kb on + strand, within EX31_RS06040 at 369.145 kb on + strand, within EX31_RS06040 at 369.145 kb on + strand, within EX31_RS06040 at 369.146 kb on - strand, within EX31_RS06040 at 369.146 kb on - strand, within EX31_RS06040 at 369.146 kb on - strand, within EX31_RS06040 at 369.182 kb on + strand, within EX31_RS06040 at 369.182 kb on + strand, within EX31_RS06040 at 369.183 kb on - strand, within EX31_RS06040 at 369.183 kb on - strand, within EX31_RS06040 at 369.183 kb on - strand, within EX31_RS06040 at 369.262 kb on + strand, within EX31_RS06040 at 369.262 kb on + strand, within EX31_RS06040 at 369.263 kb on - strand, within EX31_RS06040 at 369.263 kb on - strand, within EX31_RS06040 at 369.263 kb on - strand, within EX31_RS06040 at 369.369 kb on + strand, within EX31_RS06040 at 369.387 kb on + strand, within EX31_RS06040 at 369.387 kb on + strand, within EX31_RS06040 at 369.388 kb on - strand, within EX31_RS06040 at 369.388 kb on - strand, within EX31_RS06040 at 369.388 kb on - strand, within EX31_RS06040 at 369.388 kb on - strand, within EX31_RS06040 at 369.431 kb on + strand, within EX31_RS06040 at 369.432 kb on - strand, within EX31_RS06040 at 369.479 kb on + strand, within EX31_RS06040 at 369.479 kb on + strand, within EX31_RS06040 at 369.479 kb on + strand, within EX31_RS06040 at 369.480 kb on - strand, within EX31_RS06040 at 369.480 kb on - strand, within EX31_RS06040 at 369.656 kb on - strand, within EX31_RS06040 at 369.656 kb on - strand, within EX31_RS06040 at 369.754 kb on + strand at 369.754 kb on + strand at 369.754 kb on + strand at 369.755 kb on - strand at 369.755 kb on - strand at 369.755 kb on - strand at 369.755 kb on - strand at 369.755 kb on - strand at 369.761 kb on + strand at 369.761 kb on + strand at 369.761 kb on + strand at 369.762 kb on - strand at 369.784 kb on + strand at 369.784 kb on + strand at 369.784 kb on + strand at 369.784 kb on + strand at 369.784 kb on + strand at 369.784 kb on + strand at 369.785 kb on - strand at 369.785 kb on - strand at 369.785 kb on - strand at 369.785 kb on - strand at 369.785 kb on - strand at 369.785 kb on - strand at 369.785 kb on - strand at 369.795 kb on + strand at 369.796 kb on - strand at 369.809 kb on + strand at 369.809 kb on + strand at 369.809 kb on + strand at 369.810 kb on - strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.813 kb on + strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.814 kb on - strand at 369.818 kb on + strand at 369.818 kb on + strand at 369.819 kb on - strand at 369.860 kb on + strand at 369.860 kb on + strand at 369.860 kb on + strand at 369.860 kb on + strand at 369.860 kb on + strand at 369.860 kb on + strand at 369.860 kb on + strand at 369.861 kb on - strand at 369.861 kb on - strand at 369.861 kb on - strand at 369.861 kb on - strand at 369.877 kb on + strand at 369.878 kb on - strand at 369.878 kb on - strand at 369.933 kb on + strand at 369.933 kb on + strand at 369.933 kb on + strand at 369.933 kb on + strand at 369.934 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 367,394 - EX31_RS06030 0.44 +0.3 367,467 + EX31_RS06030 0.49 +2.0 367,467 + EX31_RS06030 0.49 +0.8 367,467 + EX31_RS06030 0.49 -0.6 367,467 + EX31_RS06030 0.49 +0.6 367,467 + EX31_RS06030 0.49 +1.5 367,468 - EX31_RS06030 0.49 -0.2 367,468 - EX31_RS06030 0.49 -0.7 367,468 - EX31_RS06030 0.49 -0.1 367,468 - EX31_RS06030 0.49 +0.1 367,468 - EX31_RS06030 0.49 -0.4 367,468 - EX31_RS06030 0.49 +1.6 367,510 + EX31_RS06030 0.53 +0.6 367,511 - EX31_RS06030 0.53 -0.2 367,610 - EX31_RS06030 0.60 -1.1 367,645 + EX31_RS06030 0.63 +0.6 367,645 + EX31_RS06030 0.63 +0.1 367,645 + EX31_RS06030 0.63 +1.0 367,645 + EX31_RS06030 0.63 +0.2 367,645 + EX31_RS06030 0.63 -0.8 367,646 - EX31_RS06030 0.63 +0.2 367,678 + EX31_RS06030 0.65 -0.9 367,679 - EX31_RS06030 0.65 +0.0 367,679 - EX31_RS06030 0.65 +0.4 367,712 + EX31_RS06030 0.68 -0.2 367,712 + EX31_RS06030 0.68 -1.8 367,713 - EX31_RS06030 0.68 +0.8 367,713 - EX31_RS06030 0.68 -1.3 367,713 - EX31_RS06030 0.68 -0.1 367,713 - EX31_RS06030 0.68 -1.5 368,011 + -0.2 368,013 + -1.3 368,013 + +0.5 368,013 + -0.1 368,013 + +1.3 368,013 + -0.9 368,013 + -0.7 368,013 + -0.6 368,013 + -0.3 368,014 - +0.5 368,014 - +0.7 368,014 - +0.9 368,014 - -0.2 368,014 - -0.9 368,014 - -0.1 368,014 - -0.6 368,014 - +0.1 368,032 + -1.0 368,032 + -1.2 368,032 + -1.3 368,033 - +0.0 368,033 - -0.1 368,033 - -0.5 368,033 - +1.1 368,033 - -0.2 368,033 - -0.6 368,033 - +0.5 368,033 - +0.8 368,033 - +0.2 368,036 + -2.3 368,037 - -2.2 368,060 + -1.0 368,061 - +0.1 368,087 - +0.1 368,187 - +0.1 368,196 - +0.0 368,221 + -0.0 368,221 + -0.0 368,221 + -0.1 368,222 - -0.2 368,222 - +0.4 368,222 - +0.8 368,230 - +0.2 368,235 + -0.7 368,236 - +0.5 368,236 - -0.1 368,236 - -0.5 368,246 + +0.0 368,256 + -0.0 368,256 + -1.2 368,269 + +0.5 368,269 + +0.8 368,269 + +0.0 368,270 - -0.2 368,270 - -2.2 368,295 + +0.1 368,295 + -0.4 368,295 + +0.5 368,295 + +2.4 368,295 + +0.3 368,295 + +0.3 368,295 + +0.6 368,295 + -2.5 368,295 + +1.1 368,295 + +0.6 368,295 + -0.5 368,295 + -0.1 368,296 - +1.0 368,296 - -0.8 368,296 - +0.0 368,296 - +0.2 368,371 + EX31_RS06035 0.11 -0.7 368,371 + EX31_RS06035 0.11 -0.3 368,371 + EX31_RS06035 0.11 +0.1 368,371 + EX31_RS06035 0.11 -0.0 368,371 + EX31_RS06035 0.11 -0.4 368,372 - EX31_RS06035 0.11 +0.6 368,372 - EX31_RS06035 0.11 -1.4 368,372 - EX31_RS06035 0.11 -1.1 368,423 - EX31_RS06035 0.19 +1.2 368,431 + EX31_RS06035 0.20 -2.3 368,431 + EX31_RS06035 0.20 -0.2 368,473 - EX31_RS06035 0.27 -0.6 368,562 + EX31_RS06035 0.40 +0.4 368,562 + EX31_RS06035 0.40 +0.0 368,562 + EX31_RS06035 0.40 +0.1 368,562 + EX31_RS06035 0.40 -0.1 368,562 + EX31_RS06035 0.40 +0.4 368,563 - EX31_RS06035 0.40 +0.0 368,563 - EX31_RS06035 0.40 +0.3 368,563 - EX31_RS06035 0.40 -0.3 368,563 - EX31_RS06035 0.40 +0.6 368,563 - EX31_RS06035 0.40 -1.6 368,563 - EX31_RS06035 0.40 +0.4 368,563 - EX31_RS06035 0.40 +0.1 368,563 - EX31_RS06035 0.40 +0.4 368,563 - EX31_RS06035 0.40 +0.3 368,563 - EX31_RS06035 0.40 -2.2 368,563 - EX31_RS06035 0.40 -0.9 368,583 - EX31_RS06035 0.43 -0.2 368,583 - EX31_RS06035 0.43 -0.5 368,666 + EX31_RS06035 0.56 +0.9 368,677 - EX31_RS06035 0.57 -0.0 368,685 + EX31_RS06035 0.58 -0.0 368,685 + EX31_RS06035 0.58 +1.5 368,685 + EX31_RS06035 0.58 +0.2 368,685 + EX31_RS06035 0.58 +0.6 368,740 + EX31_RS06035 0.67 -0.9 368,740 + EX31_RS06035 0.67 -0.4 368,740 + EX31_RS06035 0.67 +0.2 368,740 + EX31_RS06035 0.67 +0.5 368,740 + EX31_RS06035 0.67 +0.2 368,845 + EX31_RS06035 0.82 +0.1 368,846 - EX31_RS06035 0.83 +1.5 368,849 - EX31_RS06035 0.83 +0.2 368,849 - EX31_RS06035 0.83 -0.8 368,865 + EX31_RS06035 0.85 +1.2 369,037 + EX31_RS06040 0.10 +1.1 369,037 + EX31_RS06040 0.10 -0.3 369,037 + EX31_RS06040 0.10 +0.5 369,037 + EX31_RS06040 0.10 -1.8 369,037 + EX31_RS06040 0.10 -0.1 369,038 - EX31_RS06040 0.10 +0.4 369,080 - EX31_RS06040 0.16 +0.3 369,114 - EX31_RS06040 0.20 +0.1 369,141 + EX31_RS06040 0.23 +0.6 369,145 + EX31_RS06040 0.23 +0.6 369,145 + EX31_RS06040 0.23 +0.5 369,146 - EX31_RS06040 0.23 +0.1 369,146 - EX31_RS06040 0.23 -0.7 369,146 - EX31_RS06040 0.23 -0.2 369,182 + EX31_RS06040 0.28 -1.4 369,182 + EX31_RS06040 0.28 +0.1 369,183 - EX31_RS06040 0.28 +0.9 369,183 - EX31_RS06040 0.28 +0.8 369,183 - EX31_RS06040 0.28 -0.5 369,262 + EX31_RS06040 0.37 +0.0 369,262 + EX31_RS06040 0.37 -0.8 369,263 - EX31_RS06040 0.38 -0.5 369,263 - EX31_RS06040 0.38 +1.0 369,263 - EX31_RS06040 0.38 +1.2 369,369 + EX31_RS06040 0.50 -0.2 369,387 + EX31_RS06040 0.52 +0.5 369,387 + EX31_RS06040 0.52 +0.1 369,388 - EX31_RS06040 0.53 +0.3 369,388 - EX31_RS06040 0.53 -0.5 369,388 - EX31_RS06040 0.53 +0.1 369,388 - EX31_RS06040 0.53 +0.3 369,431 + EX31_RS06040 0.58 -0.8 369,432 - EX31_RS06040 0.58 +1.1 369,479 + EX31_RS06040 0.64 -2.0 369,479 + EX31_RS06040 0.64 -0.1 369,479 + EX31_RS06040 0.64 +0.4 369,480 - EX31_RS06040 0.64 +0.1 369,480 - EX31_RS06040 0.64 +0.2 369,656 - EX31_RS06040 0.85 -0.4 369,656 - EX31_RS06040 0.85 +0.5 369,754 + +0.3 369,754 + +0.3 369,754 + +0.5 369,755 - -0.3 369,755 - -0.3 369,755 - +1.2 369,755 - -0.5 369,755 - -0.6 369,761 + -0.2 369,761 + +0.3 369,761 + +0.7 369,762 - -0.7 369,784 + +0.3 369,784 + +0.8 369,784 + +1.6 369,784 + -0.1 369,784 + -0.8 369,784 + +0.3 369,785 - +0.8 369,785 - +0.2 369,785 - +0.2 369,785 - -0.8 369,785 - -0.6 369,785 - -0.1 369,785 - +0.9 369,795 + +0.6 369,796 - -0.5 369,809 + +1.2 369,809 + +1.5 369,809 + +0.1 369,810 - -0.1 369,813 + +2.1 369,813 + +1.0 369,813 + -0.4 369,813 + -0.0 369,813 + -0.0 369,813 + +0.3 369,813 + +0.2 369,813 + +0.9 369,813 + +0.6 369,813 + +0.2 369,813 + +1.8 369,813 + +0.9 369,814 - -0.5 369,814 - +0.4 369,814 - -0.3 369,814 - +0.7 369,814 - -0.1 369,814 - -0.1 369,814 - -1.1 369,814 - -0.4 369,814 - -2.4 369,814 - +0.2 369,814 - -0.0 369,814 - +0.3 369,814 - -0.3 369,818 + -0.4 369,818 + +0.5 369,819 - -0.5 369,860 + +0.5 369,860 + -0.7 369,860 + -0.4 369,860 + -0.1 369,860 + -0.4 369,860 + +0.2 369,860 + +0.2 369,861 - -0.3 369,861 - +0.5 369,861 - +0.1 369,861 - +0.9 369,877 + +1.5 369,878 - -0.5 369,878 - +0.5 369,933 + +0.4 369,933 + -1.3 369,933 + -0.2 369,933 + +0.6 369,934 - +0.5
Or see this region's nucleotide sequence