Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04905 and EX31_RS04910 are separated by 307 nucleotides EX31_RS04910 and EX31_RS04915 are separated by 220 nucleotides EX31_RS04915 and EX31_RS04920 are separated by 46 nucleotides EX31_RS04920 and EX31_RS04925 are separated by 97 nucleotides
EX31_RS04905: EX31_RS04905 - malonate decarboxylase subunit alpha, at 106,411 to 108,072
_RS04905
EX31_RS04910: EX31_RS04910 - DUF1471 domain-containing protein, at 108,380 to 108,649
_RS04910
EX31_RS04915: EX31_RS04915 - pimeloyl-ACP methyl ester esterase BioH, at 108,870 to 109,652
_RS04915
EX31_RS04920: EX31_RS04920 - DNA utilization protein GntX, at 109,699 to 110,388
_RS04920
EX31_RS04925: EX31_RS04925 - Fe-S biogenesis protein NfuA, at 110,486 to 111,061
_RS04925
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 107.902 kb on - strand, within EX31_RS04905 at 107.956 kb on - strand at 107.961 kb on + strand at 107.962 kb on - strand at 107.962 kb on - strand at 107.962 kb on - strand at 107.971 kb on + strand at 107.980 kb on - strand at 108.009 kb on + strand at 108.189 kb on + strand at 108.190 kb on - strand at 108.210 kb on - strand at 108.222 kb on - strand at 108.222 kb on - strand at 108.265 kb on + strand at 108.266 kb on - strand at 108.266 kb on - strand at 108.382 kb on - strand at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.441 kb on + strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.442 kb on - strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.444 kb on + strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.445 kb on - strand, within EX31_RS04910 at 108.507 kb on + strand, within EX31_RS04910 at 108.507 kb on + strand, within EX31_RS04910 at 108.507 kb on + strand, within EX31_RS04910 at 108.507 kb on + strand, within EX31_RS04910 at 108.507 kb on + strand, within EX31_RS04910 at 108.507 kb on + strand, within EX31_RS04910 at 108.507 kb on + strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.508 kb on - strand, within EX31_RS04910 at 108.543 kb on + strand, within EX31_RS04910 at 108.543 kb on + strand, within EX31_RS04910 at 108.544 kb on - strand, within EX31_RS04910 at 108.551 kb on - strand, within EX31_RS04910 at 108.551 kb on - strand, within EX31_RS04910 at 108.606 kb on - strand, within EX31_RS04910 at 108.631 kb on + strand at 108.632 kb on - strand at 108.654 kb on + strand at 108.654 kb on + strand at 108.654 kb on + strand at 108.654 kb on + strand at 108.654 kb on + strand at 108.655 kb on - strand at 108.655 kb on - strand at 108.655 kb on - strand at 108.655 kb on - strand at 108.674 kb on + strand at 108.674 kb on + strand at 108.675 kb on - strand at 108.742 kb on - strand at 108.783 kb on - strand at 108.790 kb on + strand at 108.790 kb on + strand at 108.790 kb on + strand at 108.790 kb on + strand at 108.790 kb on + strand at 108.790 kb on + strand at 108.790 kb on + strand at 108.790 kb on + strand at 108.791 kb on - strand at 108.791 kb on - strand at 108.791 kb on - strand at 108.822 kb on + strand at 108.823 kb on - strand at 108.823 kb on - strand at 108.823 kb on - strand at 108.823 kb on - strand at 108.823 kb on - strand at 108.826 kb on + strand at 108.826 kb on + strand at 108.826 kb on + strand at 108.826 kb on + strand at 108.826 kb on + strand at 108.826 kb on + strand at 108.826 kb on + strand at 108.826 kb on + strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.827 kb on - strand at 108.829 kb on + strand at 108.829 kb on + strand at 108.846 kb on + strand at 108.847 kb on - strand at 108.871 kb on + strand at 108.872 kb on - strand at 109.045 kb on + strand, within EX31_RS04915 at 109.046 kb on - strand, within EX31_RS04915 at 109.046 kb on - strand, within EX31_RS04915 at 109.049 kb on + strand, within EX31_RS04915 at 109.104 kb on + strand, within EX31_RS04915 at 109.104 kb on + strand, within EX31_RS04915 at 109.257 kb on + strand, within EX31_RS04915 at 109.257 kb on + strand, within EX31_RS04915 at 109.257 kb on + strand, within EX31_RS04915 at 109.257 kb on + strand, within EX31_RS04915 at 109.258 kb on - strand, within EX31_RS04915 at 109.258 kb on - strand, within EX31_RS04915 at 109.258 kb on - strand, within EX31_RS04915 at 109.258 kb on - strand, within EX31_RS04915 at 109.258 kb on - strand, within EX31_RS04915 at 109.281 kb on + strand, within EX31_RS04915 at 109.281 kb on + strand, within EX31_RS04915 at 109.281 kb on + strand, within EX31_RS04915 at 109.282 kb on - strand, within EX31_RS04915 at 109.282 kb on - strand, within EX31_RS04915 at 109.282 kb on - strand, within EX31_RS04915 at 109.282 kb on - strand, within EX31_RS04915 at 109.282 kb on - strand, within EX31_RS04915 at 109.282 kb on - strand, within EX31_RS04915 at 109.343 kb on + strand, within EX31_RS04915 at 109.419 kb on + strand, within EX31_RS04915 at 109.419 kb on + strand, within EX31_RS04915 at 109.419 kb on + strand, within EX31_RS04915 at 109.420 kb on - strand, within EX31_RS04915 at 109.420 kb on - strand, within EX31_RS04915 at 109.420 kb on - strand, within EX31_RS04915 at 109.420 kb on - strand, within EX31_RS04915 at 109.420 kb on - strand, within EX31_RS04915 at 109.505 kb on - strand, within EX31_RS04915 at 109.646 kb on + strand at 109.646 kb on + strand at 109.646 kb on + strand at 109.647 kb on - strand at 109.647 kb on - strand at 109.647 kb on - strand at 109.652 kb on + strand at 109.652 kb on + strand at 109.653 kb on - strand at 109.653 kb on - strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.657 kb on + strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.658 kb on - strand at 109.703 kb on + strand at 109.703 kb on + strand at 109.704 kb on - strand at 109.704 kb on - strand at 109.727 kb on + strand at 109.728 kb on - strand at 109.728 kb on - strand at 109.865 kb on + strand, within EX31_RS04920 at 109.865 kb on + strand, within EX31_RS04920 at 109.866 kb on - strand, within EX31_RS04920 at 109.907 kb on - strand, within EX31_RS04920 at 109.907 kb on - strand, within EX31_RS04920 at 109.985 kb on + strand, within EX31_RS04920 at 109.986 kb on - strand, within EX31_RS04920 at 110.075 kb on - strand, within EX31_RS04920 at 110.092 kb on - strand, within EX31_RS04920 at 110.092 kb on - strand, within EX31_RS04920 at 110.092 kb on - strand, within EX31_RS04920 at 110.092 kb on - strand, within EX31_RS04920 at 110.143 kb on + strand, within EX31_RS04920 at 110.143 kb on + strand, within EX31_RS04920 at 110.144 kb on - strand, within EX31_RS04920 at 110.302 kb on - strand, within EX31_RS04920 at 110.331 kb on + strand at 110.364 kb on + strand at 110.365 kb on - strand at 110.365 kb on - strand at 110.365 kb on - strand at 110.365 kb on - strand at 110.378 kb on + strand at 110.379 kb on - strand at 110.386 kb on + strand at 110.386 kb on + strand at 110.387 kb on - strand at 110.400 kb on + strand at 110.400 kb on + strand at 110.400 kb on + strand at 110.401 kb on - strand at 110.445 kb on + strand at 110.445 kb on + strand at 110.445 kb on + strand at 110.446 kb on - strand at 110.446 kb on - strand at 110.446 kb on - strand at 110.458 kb on + strand at 110.458 kb on + strand at 110.459 kb on - strand at 110.459 kb on - strand at 110.459 kb on - strand at 110.463 kb on + strand at 110.471 kb on + strand at 110.485 kb on + strand at 110.486 kb on - strand at 110.494 kb on + strand at 110.495 kb on - strand at 110.612 kb on + strand, within EX31_RS04925 at 110.631 kb on + strand, within EX31_RS04925 at 110.632 kb on - strand, within EX31_RS04925
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 107,902 - EX31_RS04905 0.90 -0.2 107,956 - +0.8 107,961 + -0.2 107,962 - +0.1 107,962 - -0.7 107,962 - +0.3 107,971 + -0.0 107,980 - +0.3 108,009 + +0.0 108,189 + +0.3 108,190 - +0.0 108,210 - +0.8 108,222 - -0.4 108,222 - -0.2 108,265 + -0.1 108,266 - -0.8 108,266 - +0.4 108,382 - +0.1 108,441 + EX31_RS04910 0.23 +0.1 108,441 + EX31_RS04910 0.23 +0.2 108,441 + EX31_RS04910 0.23 +0.2 108,441 + EX31_RS04910 0.23 -1.1 108,441 + EX31_RS04910 0.23 +1.1 108,441 + EX31_RS04910 0.23 -0.3 108,441 + EX31_RS04910 0.23 +1.0 108,441 + EX31_RS04910 0.23 +1.2 108,441 + EX31_RS04910 0.23 +2.2 108,441 + EX31_RS04910 0.23 -0.3 108,441 + EX31_RS04910 0.23 +0.0 108,441 + EX31_RS04910 0.23 +0.3 108,441 + EX31_RS04910 0.23 -0.6 108,441 + EX31_RS04910 0.23 +0.4 108,441 + EX31_RS04910 0.23 -0.9 108,441 + EX31_RS04910 0.23 -0.5 108,442 - EX31_RS04910 0.23 +0.4 108,442 - EX31_RS04910 0.23 -0.9 108,442 - EX31_RS04910 0.23 +0.2 108,442 - EX31_RS04910 0.23 -0.8 108,442 - EX31_RS04910 0.23 +1.1 108,442 - EX31_RS04910 0.23 +0.2 108,442 - EX31_RS04910 0.23 +0.1 108,442 - EX31_RS04910 0.23 -0.3 108,442 - EX31_RS04910 0.23 +0.1 108,442 - EX31_RS04910 0.23 -0.1 108,444 + EX31_RS04910 0.24 -0.4 108,444 + EX31_RS04910 0.24 -1.2 108,444 + EX31_RS04910 0.24 -0.1 108,444 + EX31_RS04910 0.24 -0.9 108,444 + EX31_RS04910 0.24 +0.6 108,444 + EX31_RS04910 0.24 +1.9 108,444 + EX31_RS04910 0.24 -2.2 108,444 + EX31_RS04910 0.24 -1.1 108,444 + EX31_RS04910 0.24 -0.4 108,444 + EX31_RS04910 0.24 +0.5 108,445 - EX31_RS04910 0.24 +0.0 108,445 - EX31_RS04910 0.24 +1.7 108,445 - EX31_RS04910 0.24 +0.5 108,445 - EX31_RS04910 0.24 +0.2 108,445 - EX31_RS04910 0.24 +0.1 108,445 - EX31_RS04910 0.24 +0.2 108,445 - EX31_RS04910 0.24 -0.2 108,445 - EX31_RS04910 0.24 +0.2 108,445 - EX31_RS04910 0.24 +0.3 108,445 - EX31_RS04910 0.24 -0.7 108,445 - EX31_RS04910 0.24 +0.3 108,445 - EX31_RS04910 0.24 +0.4 108,507 + EX31_RS04910 0.47 +0.4 108,507 + EX31_RS04910 0.47 -0.3 108,507 + EX31_RS04910 0.47 +0.8 108,507 + EX31_RS04910 0.47 -0.7 108,507 + EX31_RS04910 0.47 +0.1 108,507 + EX31_RS04910 0.47 +0.8 108,507 + EX31_RS04910 0.47 +0.3 108,508 - EX31_RS04910 0.47 -0.6 108,508 - EX31_RS04910 0.47 +0.2 108,508 - EX31_RS04910 0.47 +0.3 108,508 - EX31_RS04910 0.47 +1.0 108,508 - EX31_RS04910 0.47 +0.9 108,508 - EX31_RS04910 0.47 -0.6 108,508 - EX31_RS04910 0.47 +0.1 108,508 - EX31_RS04910 0.47 -0.2 108,543 + EX31_RS04910 0.60 +1.2 108,543 + EX31_RS04910 0.60 -0.0 108,544 - EX31_RS04910 0.61 +0.3 108,551 - EX31_RS04910 0.63 +0.2 108,551 - EX31_RS04910 0.63 +0.3 108,606 - EX31_RS04910 0.84 +0.6 108,631 + +0.7 108,632 - +0.2 108,654 + -0.5 108,654 + +0.9 108,654 + +2.3 108,654 + +0.3 108,654 + -1.3 108,655 - +0.1 108,655 - -0.3 108,655 - +0.6 108,655 - +0.6 108,674 + +0.1 108,674 + +1.1 108,675 - +0.2 108,742 - -0.2 108,783 - +0.3 108,790 + -0.9 108,790 + +0.1 108,790 + +0.7 108,790 + -0.3 108,790 + +0.6 108,790 + -1.0 108,790 + -1.7 108,790 + +1.4 108,791 - +0.3 108,791 - +0.2 108,791 - +0.4 108,822 + +0.2 108,823 - -1.3 108,823 - -0.4 108,823 - +0.6 108,823 - +0.1 108,823 - +0.9 108,826 + -0.2 108,826 + +0.2 108,826 + +0.9 108,826 + -0.3 108,826 + -0.3 108,826 + +0.2 108,826 + +1.3 108,826 + -0.1 108,827 - -1.0 108,827 - -0.3 108,827 - -0.1 108,827 - +0.7 108,827 - +0.1 108,827 - -0.2 108,827 - +0.6 108,827 - +0.3 108,827 - +0.5 108,829 + -0.1 108,829 + -1.4 108,846 + +0.3 108,847 - +0.0 108,871 + +0.4 108,872 - -0.3 109,045 + EX31_RS04915 0.22 +0.9 109,046 - EX31_RS04915 0.22 -1.2 109,046 - EX31_RS04915 0.22 -0.3 109,049 + EX31_RS04915 0.23 +0.2 109,104 + EX31_RS04915 0.30 +0.2 109,104 + EX31_RS04915 0.30 +0.8 109,257 + EX31_RS04915 0.49 -0.2 109,257 + EX31_RS04915 0.49 +0.3 109,257 + EX31_RS04915 0.49 -0.7 109,257 + EX31_RS04915 0.49 -0.5 109,258 - EX31_RS04915 0.50 -0.3 109,258 - EX31_RS04915 0.50 +0.4 109,258 - EX31_RS04915 0.50 -0.7 109,258 - EX31_RS04915 0.50 +0.2 109,258 - EX31_RS04915 0.50 +1.6 109,281 + EX31_RS04915 0.52 +0.2 109,281 + EX31_RS04915 0.52 -0.3 109,281 + EX31_RS04915 0.52 +0.4 109,282 - EX31_RS04915 0.53 +0.2 109,282 - EX31_RS04915 0.53 +0.0 109,282 - EX31_RS04915 0.53 -0.2 109,282 - EX31_RS04915 0.53 +0.0 109,282 - EX31_RS04915 0.53 -0.0 109,282 - EX31_RS04915 0.53 +0.2 109,343 + EX31_RS04915 0.60 -0.9 109,419 + EX31_RS04915 0.70 -1.4 109,419 + EX31_RS04915 0.70 +0.0 109,419 + EX31_RS04915 0.70 -0.9 109,420 - EX31_RS04915 0.70 -0.3 109,420 - EX31_RS04915 0.70 -1.0 109,420 - EX31_RS04915 0.70 -0.4 109,420 - EX31_RS04915 0.70 -1.1 109,420 - EX31_RS04915 0.70 +1.6 109,505 - EX31_RS04915 0.81 +0.5 109,646 + +0.2 109,646 + -0.2 109,646 + +0.1 109,647 - -0.5 109,647 - +0.5 109,647 - -0.2 109,652 + -0.8 109,652 + -0.5 109,653 - +0.5 109,653 - +0.2 109,657 + +1.1 109,657 + -0.6 109,657 + +0.3 109,657 + -0.4 109,657 + +1.0 109,657 + -0.3 109,657 + +0.6 109,657 + -0.7 109,657 + -0.8 109,657 + +2.6 109,658 - -0.7 109,658 - -0.4 109,658 - -0.2 109,658 - -0.1 109,658 - +0.4 109,658 - +0.5 109,658 - +0.4 109,658 - -0.8 109,658 - +0.1 109,703 + +0.1 109,703 + -0.7 109,704 - +1.1 109,704 - -0.9 109,727 + +1.0 109,728 - -0.1 109,728 - -0.3 109,865 + EX31_RS04920 0.24 -0.9 109,865 + EX31_RS04920 0.24 -0.8 109,866 - EX31_RS04920 0.24 -0.0 109,907 - EX31_RS04920 0.30 -0.1 109,907 - EX31_RS04920 0.30 -0.6 109,985 + EX31_RS04920 0.41 +2.6 109,986 - EX31_RS04920 0.42 +0.2 110,075 - EX31_RS04920 0.54 -0.2 110,092 - EX31_RS04920 0.57 -0.7 110,092 - EX31_RS04920 0.57 +1.1 110,092 - EX31_RS04920 0.57 +0.9 110,092 - EX31_RS04920 0.57 -0.0 110,143 + EX31_RS04920 0.64 -0.1 110,143 + EX31_RS04920 0.64 +0.3 110,144 - EX31_RS04920 0.64 -1.0 110,302 - EX31_RS04920 0.87 +0.2 110,331 + +0.1 110,364 + +1.3 110,365 - +1.7 110,365 - -0.0 110,365 - +0.1 110,365 - +0.8 110,378 + +0.7 110,379 - +1.8 110,386 + -1.2 110,386 + -0.1 110,387 - -0.5 110,400 + +1.0 110,400 + -1.2 110,400 + -0.3 110,401 - -0.3 110,445 + +0.3 110,445 + -0.6 110,445 + -0.2 110,446 - +0.5 110,446 - +2.2 110,446 - -0.4 110,458 + -0.0 110,458 + +0.2 110,459 - -0.5 110,459 - +0.3 110,459 - +0.6 110,463 + +0.1 110,471 + +0.2 110,485 + -1.8 110,486 - -1.1 110,494 + -0.6 110,495 - +0.3 110,612 + EX31_RS04925 0.22 -0.3 110,631 + EX31_RS04925 0.25 +0.4 110,632 - EX31_RS04925 0.25 +1.4
Or see this region's nucleotide sequence