Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02710 and EX31_RS02715 are separated by 81 nucleotides EX31_RS02715 and EX31_RS02720 are separated by 24 nucleotides EX31_RS02720 and EX31_RS02725 are separated by 192 nucleotides EX31_RS02725 and EX31_RS02730 overlap by 1 nucleotides
EX31_RS02710: EX31_RS02710 - radical SAM family heme chaperone HemW, at 12,500 to 13,636
_RS02710
EX31_RS02715: EX31_RS02715 - DUF2884 domain-containing protein, at 13,718 to 14,452
_RS02715
EX31_RS02720: EX31_RS02720 - glutaminase B, at 14,477 to 15,403
_RS02720
EX31_RS02725: EX31_RS02725 - YggL family protein, at 15,596 to 15,922
_RS02725
EX31_RS02730: EX31_RS02730 - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 15,922 to 16,641
_RS02730
Position (kb) 14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 13.487 kb on + strand, within EX31_RS02710 at 13.487 kb on + strand, within EX31_RS02710 at 13.487 kb on + strand, within EX31_RS02710 at 13.487 kb on + strand, within EX31_RS02710 at 13.487 kb on + strand, within EX31_RS02710 at 13.488 kb on - strand, within EX31_RS02710 at 13.488 kb on - strand, within EX31_RS02710 at 13.547 kb on + strand at 13.547 kb on + strand at 13.547 kb on + strand at 13.548 kb on - strand at 13.596 kb on + strand at 13.634 kb on + strand at 13.634 kb on + strand at 13.634 kb on + strand at 13.634 kb on + strand at 13.635 kb on - strand at 13.684 kb on - strand at 13.712 kb on - strand at 13.720 kb on - strand at 13.778 kb on - strand at 13.862 kb on - strand, within EX31_RS02715 at 14.000 kb on - strand, within EX31_RS02715 at 14.000 kb on - strand, within EX31_RS02715 at 14.087 kb on - strand, within EX31_RS02715 at 14.133 kb on - strand, within EX31_RS02715 at 14.336 kb on - strand, within EX31_RS02715 at 14.336 kb on - strand, within EX31_RS02715 at 14.336 kb on - strand, within EX31_RS02715 at 14.354 kb on - strand, within EX31_RS02715 at 14.354 kb on - strand, within EX31_RS02715 at 14.361 kb on - strand, within EX31_RS02715 at 14.374 kb on - strand, within EX31_RS02715 at 14.374 kb on - strand, within EX31_RS02715 at 14.374 kb on - strand, within EX31_RS02715 at 14.448 kb on - strand at 14.448 kb on - strand at 14.454 kb on + strand at 14.454 kb on + strand at 14.455 kb on - strand at 14.455 kb on - strand at 14.455 kb on - strand at 14.455 kb on - strand at 14.455 kb on - strand at 14.455 kb on - strand at 14.455 kb on - strand at 14.455 kb on - strand at 14.465 kb on - strand at 14.497 kb on + strand at 14.497 kb on + strand at 14.498 kb on - strand at 14.516 kb on + strand at 14.517 kb on - strand at 14.517 kb on - strand at 14.517 kb on - strand at 14.517 kb on - strand at 14.517 kb on - strand at 14.517 kb on - strand at 14.527 kb on + strand at 14.527 kb on + strand at 14.528 kb on - strand at 14.528 kb on - strand at 14.528 kb on - strand at 14.528 kb on - strand at 14.571 kb on - strand, within EX31_RS02720 at 14.605 kb on + strand, within EX31_RS02720 at 14.606 kb on - strand, within EX31_RS02720 at 14.606 kb on - strand, within EX31_RS02720 at 14.606 kb on - strand, within EX31_RS02720 at 14.606 kb on - strand, within EX31_RS02720 at 14.606 kb on - strand, within EX31_RS02720 at 14.608 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.673 kb on + strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.674 kb on - strand, within EX31_RS02720 at 14.677 kb on + strand, within EX31_RS02720 at 14.678 kb on - strand, within EX31_RS02720 at 14.678 kb on - strand, within EX31_RS02720 at 14.678 kb on - strand, within EX31_RS02720 at 14.701 kb on + strand, within EX31_RS02720 at 14.762 kb on - strand, within EX31_RS02720 at 14.772 kb on + strand, within EX31_RS02720 at 14.772 kb on + strand, within EX31_RS02720 at 14.773 kb on - strand, within EX31_RS02720 at 14.773 kb on - strand, within EX31_RS02720 at 14.829 kb on + strand, within EX31_RS02720 at 14.829 kb on + strand, within EX31_RS02720 at 14.829 kb on + strand, within EX31_RS02720 at 14.830 kb on - strand, within EX31_RS02720 at 14.830 kb on - strand, within EX31_RS02720 at 14.830 kb on - strand, within EX31_RS02720 at 14.887 kb on - strand, within EX31_RS02720 at 14.946 kb on + strand, within EX31_RS02720 at 14.947 kb on - strand, within EX31_RS02720 at 15.029 kb on - strand, within EX31_RS02720 at 15.076 kb on + strand, within EX31_RS02720 at 15.077 kb on - strand, within EX31_RS02720 at 15.166 kb on - strand, within EX31_RS02720 at 15.266 kb on - strand, within EX31_RS02720 at 15.266 kb on - strand, within EX31_RS02720 at 15.319 kb on - strand at 15.319 kb on - strand at 15.341 kb on + strand at 15.342 kb on - strand at 15.368 kb on - strand at 15.368 kb on - strand at 15.368 kb on - strand at 15.368 kb on - strand at 15.421 kb on + strand at 15.421 kb on + strand at 15.421 kb on + strand at 15.421 kb on + strand at 15.421 kb on + strand at 15.421 kb on + strand at 15.422 kb on - strand at 15.422 kb on - strand at 15.422 kb on - strand at 15.422 kb on - strand at 15.422 kb on - strand at 15.447 kb on - strand at 15.447 kb on - strand at 15.447 kb on - strand at 15.447 kb on - strand at 15.492 kb on + strand at 15.492 kb on + strand at 15.492 kb on + strand at 15.492 kb on + strand at 15.492 kb on + strand at 15.493 kb on - strand at 15.493 kb on - strand at 15.493 kb on - strand at 15.493 kb on - strand at 15.513 kb on + strand at 15.514 kb on - strand at 15.528 kb on - strand at 15.535 kb on + strand at 15.549 kb on - strand at 15.611 kb on - strand at 15.647 kb on - strand, within EX31_RS02725 at 15.739 kb on - strand, within EX31_RS02725 at 15.763 kb on + strand, within EX31_RS02725 at 15.764 kb on - strand, within EX31_RS02725 at 15.764 kb on - strand, within EX31_RS02725 at 15.764 kb on - strand, within EX31_RS02725 at 15.764 kb on - strand, within EX31_RS02725 at 15.764 kb on - strand, within EX31_RS02725 at 15.764 kb on - strand, within EX31_RS02725 at 15.849 kb on - strand, within EX31_RS02725 at 15.849 kb on - strand, within EX31_RS02725 at 15.885 kb on - strand, within EX31_RS02725 at 15.885 kb on - strand, within EX31_RS02725 at 15.892 kb on + strand at 15.893 kb on - strand at 15.893 kb on - strand at 15.893 kb on - strand at 15.893 kb on - strand at 15.893 kb on - strand at 15.896 kb on + strand at 15.923 kb on + strand at 15.924 kb on - strand at 15.924 kb on - strand at 16.022 kb on + strand, within EX31_RS02730 at 16.022 kb on + strand, within EX31_RS02730 at 16.022 kb on + strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.023 kb on - strand, within EX31_RS02730 at 16.120 kb on + strand, within EX31_RS02730 at 16.121 kb on - strand, within EX31_RS02730 at 16.121 kb on - strand, within EX31_RS02730 at 16.121 kb on - strand, within EX31_RS02730 at 16.185 kb on + strand, within EX31_RS02730 at 16.186 kb on - strand, within EX31_RS02730 at 16.186 kb on - strand, within EX31_RS02730 at 16.188 kb on + strand, within EX31_RS02730 at 16.188 kb on + strand, within EX31_RS02730 at 16.188 kb on + strand, within EX31_RS02730 at 16.189 kb on - strand, within EX31_RS02730 at 16.189 kb on - strand, within EX31_RS02730 at 16.189 kb on - strand, within EX31_RS02730 at 16.223 kb on - strand, within EX31_RS02730 at 16.303 kb on - strand, within EX31_RS02730
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 13,487 + EX31_RS02710 0.87 +0.8 13,487 + EX31_RS02710 0.87 +0.1 13,487 + EX31_RS02710 0.87 +0.1 13,487 + EX31_RS02710 0.87 -0.4 13,487 + EX31_RS02710 0.87 -0.3 13,488 - EX31_RS02710 0.87 -1.4 13,488 - EX31_RS02710 0.87 -0.3 13,547 + -1.1 13,547 + -0.8 13,547 + -0.1 13,548 - -1.2 13,596 + -0.2 13,634 + +2.2 13,634 + +0.3 13,634 + +1.1 13,634 + +1.4 13,635 - -0.4 13,684 - +0.2 13,712 - +0.5 13,720 - +2.8 13,778 - -0.1 13,862 - EX31_RS02715 0.20 +0.2 14,000 - EX31_RS02715 0.38 +0.4 14,000 - EX31_RS02715 0.38 -0.2 14,087 - EX31_RS02715 0.50 -1.3 14,133 - EX31_RS02715 0.56 +0.0 14,336 - EX31_RS02715 0.84 -0.2 14,336 - EX31_RS02715 0.84 -0.3 14,336 - EX31_RS02715 0.84 -0.3 14,354 - EX31_RS02715 0.87 -0.2 14,354 - EX31_RS02715 0.87 +0.4 14,361 - EX31_RS02715 0.87 -0.4 14,374 - EX31_RS02715 0.89 +0.4 14,374 - EX31_RS02715 0.89 +0.1 14,374 - EX31_RS02715 0.89 -0.0 14,448 - -0.6 14,448 - +0.2 14,454 + -0.3 14,454 + +0.6 14,455 - -0.4 14,455 - -0.1 14,455 - +0.4 14,455 - -0.5 14,455 - -0.4 14,455 - +0.4 14,455 - -1.0 14,455 - -0.5 14,465 - +0.2 14,497 + -2.2 14,497 + -1.3 14,498 - -2.2 14,516 + -2.5 14,517 - -2.0 14,517 - -1.6 14,517 - -0.2 14,517 - -1.4 14,517 - -1.3 14,517 - -1.8 14,527 + -0.5 14,527 + -0.1 14,528 - +0.2 14,528 - -0.5 14,528 - -1.7 14,528 - -1.6 14,571 - EX31_RS02720 0.10 -3.4 14,605 + EX31_RS02720 0.14 -1.7 14,606 - EX31_RS02720 0.14 -1.8 14,606 - EX31_RS02720 0.14 -0.2 14,606 - EX31_RS02720 0.14 -1.3 14,606 - EX31_RS02720 0.14 -0.4 14,606 - EX31_RS02720 0.14 -0.3 14,608 + EX31_RS02720 0.14 -1.5 14,673 + EX31_RS02720 0.21 -1.2 14,673 + EX31_RS02720 0.21 +0.5 14,673 + EX31_RS02720 0.21 -1.5 14,673 + EX31_RS02720 0.21 -1.2 14,673 + EX31_RS02720 0.21 -0.6 14,673 + EX31_RS02720 0.21 -0.8 14,673 + EX31_RS02720 0.21 +0.5 14,673 + EX31_RS02720 0.21 -1.2 14,673 + EX31_RS02720 0.21 -1.1 14,673 + EX31_RS02720 0.21 -1.1 14,673 + EX31_RS02720 0.21 -2.1 14,673 + EX31_RS02720 0.21 -1.8 14,673 + EX31_RS02720 0.21 -1.9 14,673 + EX31_RS02720 0.21 -1.0 14,674 - EX31_RS02720 0.21 -1.1 14,674 - EX31_RS02720 0.21 +0.1 14,674 - EX31_RS02720 0.21 -1.9 14,674 - EX31_RS02720 0.21 -1.8 14,674 - EX31_RS02720 0.21 -1.3 14,674 - EX31_RS02720 0.21 -2.2 14,674 - EX31_RS02720 0.21 +0.6 14,674 - EX31_RS02720 0.21 -1.0 14,674 - EX31_RS02720 0.21 -1.1 14,674 - EX31_RS02720 0.21 -2.8 14,674 - EX31_RS02720 0.21 +0.2 14,674 - EX31_RS02720 0.21 -1.7 14,674 - EX31_RS02720 0.21 -1.2 14,674 - EX31_RS02720 0.21 -2.1 14,674 - EX31_RS02720 0.21 -2.4 14,674 - EX31_RS02720 0.21 -1.0 14,674 - EX31_RS02720 0.21 -0.9 14,677 + EX31_RS02720 0.22 -0.8 14,678 - EX31_RS02720 0.22 -2.1 14,678 - EX31_RS02720 0.22 -0.8 14,678 - EX31_RS02720 0.22 -1.5 14,701 + EX31_RS02720 0.24 -0.1 14,762 - EX31_RS02720 0.31 +4.1 14,772 + EX31_RS02720 0.32 -0.1 14,772 + EX31_RS02720 0.32 +0.2 14,773 - EX31_RS02720 0.32 -2.5 14,773 - EX31_RS02720 0.32 -1.7 14,829 + EX31_RS02720 0.38 -0.3 14,829 + EX31_RS02720 0.38 -1.8 14,829 + EX31_RS02720 0.38 -0.4 14,830 - EX31_RS02720 0.38 -3.0 14,830 - EX31_RS02720 0.38 -1.5 14,830 - EX31_RS02720 0.38 -2.7 14,887 - EX31_RS02720 0.44 -0.2 14,946 + EX31_RS02720 0.51 -1.3 14,947 - EX31_RS02720 0.51 -0.5 15,029 - EX31_RS02720 0.60 -1.2 15,076 + EX31_RS02720 0.65 -1.6 15,077 - EX31_RS02720 0.65 -0.6 15,166 - EX31_RS02720 0.74 -2.9 15,266 - EX31_RS02720 0.85 -1.3 15,266 - EX31_RS02720 0.85 -1.8 15,319 - -0.1 15,319 - -1.2 15,341 + -0.1 15,342 - -2.4 15,368 - -2.0 15,368 - -1.3 15,368 - -2.5 15,368 - -0.5 15,421 + -0.6 15,421 + -0.5 15,421 + -0.6 15,421 + +0.4 15,421 + +1.4 15,421 + -0.6 15,422 - +0.7 15,422 - +0.3 15,422 - +1.1 15,422 - +2.0 15,422 - -0.3 15,447 - +0.5 15,447 - -0.5 15,447 - -2.2 15,447 - +0.2 15,492 + -0.6 15,492 + -2.3 15,492 + -0.8 15,492 + -0.1 15,492 + -1.9 15,493 - +0.2 15,493 - +0.2 15,493 - -0.4 15,493 - +0.5 15,513 + -0.9 15,514 - +0.9 15,528 - +1.2 15,535 + -1.6 15,549 - -0.8 15,611 - -0.4 15,647 - EX31_RS02725 0.16 -0.2 15,739 - EX31_RS02725 0.44 -0.0 15,763 + EX31_RS02725 0.51 -0.9 15,764 - EX31_RS02725 0.51 -0.2 15,764 - EX31_RS02725 0.51 -0.4 15,764 - EX31_RS02725 0.51 +0.3 15,764 - EX31_RS02725 0.51 +0.1 15,764 - EX31_RS02725 0.51 +0.1 15,764 - EX31_RS02725 0.51 +1.5 15,849 - EX31_RS02725 0.77 -0.6 15,849 - EX31_RS02725 0.77 -0.4 15,885 - EX31_RS02725 0.88 -0.1 15,885 - EX31_RS02725 0.88 +0.9 15,892 + -1.6 15,893 - +0.3 15,893 - +0.6 15,893 - +0.3 15,893 - -1.3 15,893 - -0.0 15,896 + -1.2 15,923 + -0.8 15,924 - +1.1 15,924 - -0.6 16,022 + EX31_RS02730 0.14 -1.0 16,022 + EX31_RS02730 0.14 -0.8 16,022 + EX31_RS02730 0.14 -1.7 16,023 - EX31_RS02730 0.14 +0.9 16,023 - EX31_RS02730 0.14 -0.7 16,023 - EX31_RS02730 0.14 -0.0 16,023 - EX31_RS02730 0.14 +0.0 16,023 - EX31_RS02730 0.14 -0.3 16,023 - EX31_RS02730 0.14 -1.1 16,023 - EX31_RS02730 0.14 +0.2 16,023 - EX31_RS02730 0.14 +0.5 16,120 + EX31_RS02730 0.28 +0.5 16,121 - EX31_RS02730 0.28 -0.2 16,121 - EX31_RS02730 0.28 -0.9 16,121 - EX31_RS02730 0.28 -0.0 16,185 + EX31_RS02730 0.37 +0.2 16,186 - EX31_RS02730 0.37 -0.8 16,186 - EX31_RS02730 0.37 -1.1 16,188 + EX31_RS02730 0.37 -0.9 16,188 + EX31_RS02730 0.37 -0.6 16,188 + EX31_RS02730 0.37 +0.7 16,189 - EX31_RS02730 0.37 -1.0 16,189 - EX31_RS02730 0.37 -0.1 16,189 - EX31_RS02730 0.37 -0.1 16,223 - EX31_RS02730 0.42 +0.2 16,303 - EX31_RS02730 0.53 -0.4
Or see this region's nucleotide sequence