Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02075 and EX31_RS25110 are separated by 58 nucleotides EX31_RS25110 and EX31_RS02080 are separated by 29 nucleotides EX31_RS02080 and EX31_RS02085 are separated by 222 nucleotides
EX31_RS02075: EX31_RS02075 - cytochrome d ubiquinol oxidase subunit II, at 455,362 to 456,366
_RS02075
EX31_RS25110: EX31_RS25110 - DUF2474 domain-containing protein, at 456,425 to 456,574
_RS25110
EX31_RS02080: EX31_RS02080 - FadR family transcriptional regulator, at 456,604 to 457,365
_RS02080
EX31_RS02085: EX31_RS02085 - SDR family oxidoreductase, at 457,588 to 458,328
_RS02085
Position (kb) 456
457
458 Strain fitness (log2 ratio) -2
-1
0
1
2 at 455.816 kb on + strand, within EX31_RS02075 at 455.886 kb on + strand, within EX31_RS02075 at 455.886 kb on + strand, within EX31_RS02075 at 455.886 kb on + strand, within EX31_RS02075 at 455.886 kb on + strand, within EX31_RS02075 at 455.887 kb on - strand, within EX31_RS02075 at 455.887 kb on - strand, within EX31_RS02075 at 456.088 kb on - strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.127 kb on + strand, within EX31_RS02075 at 456.128 kb on - strand, within EX31_RS02075 at 456.128 kb on - strand, within EX31_RS02075 at 456.128 kb on - strand, within EX31_RS02075 at 456.128 kb on - strand, within EX31_RS02075 at 456.128 kb on - strand, within EX31_RS02075 at 456.129 kb on + strand, within EX31_RS02075 at 456.202 kb on - strand, within EX31_RS02075 at 456.261 kb on + strand, within EX31_RS02075 at 456.261 kb on + strand, within EX31_RS02075 at 456.262 kb on - strand, within EX31_RS02075 at 456.262 kb on - strand, within EX31_RS02075 at 456.262 kb on - strand, within EX31_RS02075 at 456.262 kb on - strand, within EX31_RS02075 at 456.304 kb on + strand at 456.304 kb on + strand at 456.304 kb on + strand at 456.305 kb on - strand at 456.305 kb on - strand at 456.306 kb on + strand at 456.306 kb on + strand at 456.319 kb on + strand at 456.319 kb on + strand at 456.319 kb on + strand at 456.319 kb on + strand at 456.320 kb on - strand at 456.320 kb on - strand at 456.320 kb on - strand at 456.320 kb on - strand at 456.322 kb on + strand at 456.322 kb on + strand at 456.323 kb on - strand at 456.323 kb on - strand at 456.323 kb on - strand at 456.323 kb on - strand at 456.358 kb on + strand at 456.449 kb on - strand, within EX31_RS25110 at 456.492 kb on - strand, within EX31_RS25110 at 456.492 kb on - strand, within EX31_RS25110 at 456.507 kb on + strand, within EX31_RS25110 at 456.532 kb on - strand, within EX31_RS25110 at 456.532 kb on - strand, within EX31_RS25110 at 456.624 kb on + strand at 456.624 kb on + strand at 456.747 kb on - strand, within EX31_RS02080 at 456.751 kb on + strand, within EX31_RS02080 at 456.751 kb on + strand, within EX31_RS02080 at 456.752 kb on - strand, within EX31_RS02080 at 456.844 kb on - strand, within EX31_RS02080 at 456.914 kb on + strand, within EX31_RS02080 at 456.915 kb on - strand, within EX31_RS02080 at 456.915 kb on - strand, within EX31_RS02080 at 457.234 kb on + strand, within EX31_RS02080 at 457.390 kb on + strand at 457.439 kb on + strand at 457.440 kb on - strand at 457.519 kb on + strand at 457.583 kb on + strand at 457.583 kb on + strand at 457.583 kb on + strand at 457.583 kb on + strand at 457.583 kb on + strand at 457.583 kb on + strand at 457.583 kb on + strand at 457.583 kb on + strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.584 kb on - strand at 457.587 kb on + strand at 457.588 kb on - strand at 457.712 kb on + strand, within EX31_RS02085 at 457.712 kb on + strand, within EX31_RS02085 at 457.713 kb on - strand, within EX31_RS02085 at 457.713 kb on - strand, within EX31_RS02085 at 457.713 kb on - strand, within EX31_RS02085 at 457.720 kb on - strand, within EX31_RS02085 at 457.888 kb on - strand, within EX31_RS02085 at 458.014 kb on + strand, within EX31_RS02085 at 458.015 kb on - strand, within EX31_RS02085 at 458.068 kb on + strand, within EX31_RS02085 at 458.069 kb on - strand, within EX31_RS02085 at 458.185 kb on + strand, within EX31_RS02085 at 458.185 kb on + strand, within EX31_RS02085 at 458.257 kb on + strand at 458.258 kb on - strand at 458.281 kb on + strand at 458.281 kb on + strand at 458.281 kb on + strand at 458.282 kb on - strand at 458.282 kb on - strand at 458.282 kb on - strand at 458.304 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 455,816 + EX31_RS02075 0.45 +0.3 455,886 + EX31_RS02075 0.52 -0.5 455,886 + EX31_RS02075 0.52 -0.4 455,886 + EX31_RS02075 0.52 -0.1 455,886 + EX31_RS02075 0.52 +0.1 455,887 - EX31_RS02075 0.52 +1.4 455,887 - EX31_RS02075 0.52 +0.3 456,088 - EX31_RS02075 0.72 -2.1 456,127 + EX31_RS02075 0.76 -1.3 456,127 + EX31_RS02075 0.76 -0.6 456,127 + EX31_RS02075 0.76 +1.9 456,127 + EX31_RS02075 0.76 +1.4 456,127 + EX31_RS02075 0.76 +1.0 456,127 + EX31_RS02075 0.76 -0.3 456,127 + EX31_RS02075 0.76 +0.9 456,127 + EX31_RS02075 0.76 +0.4 456,127 + EX31_RS02075 0.76 +1.4 456,128 - EX31_RS02075 0.76 -0.1 456,128 - EX31_RS02075 0.76 -1.3 456,128 - EX31_RS02075 0.76 +0.2 456,128 - EX31_RS02075 0.76 +1.4 456,128 - EX31_RS02075 0.76 -0.0 456,129 + EX31_RS02075 0.76 +0.9 456,202 - EX31_RS02075 0.84 -0.1 456,261 + EX31_RS02075 0.89 +0.3 456,261 + EX31_RS02075 0.89 +0.6 456,262 - EX31_RS02075 0.90 +1.8 456,262 - EX31_RS02075 0.90 +1.9 456,262 - EX31_RS02075 0.90 +1.0 456,262 - EX31_RS02075 0.90 +1.1 456,304 + -0.3 456,304 + +0.6 456,304 + -1.8 456,305 - -0.5 456,305 - -1.1 456,306 + +0.1 456,306 + -0.2 456,319 + +1.1 456,319 + +0.2 456,319 + -0.4 456,319 + +1.6 456,320 - -0.3 456,320 - -0.6 456,320 - -1.4 456,320 - +1.1 456,322 + -0.6 456,322 + +0.6 456,323 - -0.3 456,323 - -0.8 456,323 - -1.4 456,323 - +0.9 456,358 + -0.7 456,449 - EX31_RS25110 0.16 +0.9 456,492 - EX31_RS25110 0.45 +2.0 456,492 - EX31_RS25110 0.45 +0.4 456,507 + EX31_RS25110 0.55 -0.9 456,532 - EX31_RS25110 0.71 +0.0 456,532 - EX31_RS25110 0.71 -0.8 456,624 + +0.6 456,624 + -0.8 456,747 - EX31_RS02080 0.19 -0.7 456,751 + EX31_RS02080 0.19 -0.6 456,751 + EX31_RS02080 0.19 -0.0 456,752 - EX31_RS02080 0.19 +0.6 456,844 - EX31_RS02080 0.31 -1.7 456,914 + EX31_RS02080 0.41 -2.3 456,915 - EX31_RS02080 0.41 -0.6 456,915 - EX31_RS02080 0.41 -0.4 457,234 + EX31_RS02080 0.83 +0.1 457,390 + +0.4 457,439 + +0.5 457,440 - +0.3 457,519 + -0.3 457,583 + +0.5 457,583 + +1.0 457,583 + -1.3 457,583 + +0.4 457,583 + -0.2 457,583 + -0.4 457,583 + -0.0 457,583 + +1.0 457,584 - +2.2 457,584 - -1.3 457,584 - -1.6 457,584 - -1.3 457,584 - -0.7 457,584 - +1.6 457,584 - -0.8 457,584 - -0.0 457,584 - +0.9 457,584 - -0.2 457,584 - +0.9 457,587 + +1.2 457,588 - +0.9 457,712 + EX31_RS02085 0.17 +1.3 457,712 + EX31_RS02085 0.17 +1.6 457,713 - EX31_RS02085 0.17 -0.5 457,713 - EX31_RS02085 0.17 -1.1 457,713 - EX31_RS02085 0.17 -0.3 457,720 - EX31_RS02085 0.18 +1.3 457,888 - EX31_RS02085 0.40 +0.1 458,014 + EX31_RS02085 0.57 +0.4 458,015 - EX31_RS02085 0.58 -0.3 458,068 + EX31_RS02085 0.65 +1.2 458,069 - EX31_RS02085 0.65 +0.7 458,185 + EX31_RS02085 0.81 +0.7 458,185 + EX31_RS02085 0.81 -1.1 458,257 + -0.8 458,258 - +0.6 458,281 + -1.7 458,281 + -0.9 458,281 + +1.3 458,282 - -0.6 458,282 - +0.5 458,282 - -0.1 458,304 + +0.7
Or see this region's nucleotide sequence