Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01250 and EX31_RS01255 are separated by 134 nucleotides EX31_RS01255 and EX31_RS01260 are separated by 52 nucleotides EX31_RS01260 and EX31_RS01265 are separated by 202 nucleotides
EX31_RS01250: EX31_RS01250 - M24 family metallopeptidase, at 279,090 to 280,484
_RS01250
EX31_RS01255: EX31_RS01255 - Crp/Fnr family transcriptional regulator, at 280,619 to 281,251
_RS01255
EX31_RS01260: EX31_RS01260 - YebG family protein, at 281,304 to 281,699
_RS01260
EX31_RS01265: EX31_RS01265 - GNAT family N-acetyltransferase, at 281,902 to 282,357
_RS01265
Position (kb) 280
281
282 Strain fitness (log2 ratio) -2
-1
0
1
2 at 279.619 kb on + strand, within EX31_RS01250 at 279.620 kb on - strand, within EX31_RS01250 at 279.671 kb on + strand, within EX31_RS01250 at 279.780 kb on + strand, within EX31_RS01250 at 279.893 kb on + strand, within EX31_RS01250 at 279.893 kb on + strand, within EX31_RS01250 at 279.894 kb on - strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.294 kb on + strand, within EX31_RS01250 at 280.327 kb on + strand, within EX31_RS01250 at 280.327 kb on + strand, within EX31_RS01250 at 280.328 kb on - strand, within EX31_RS01250 at 280.433 kb on - strand at 280.433 kb on - strand at 280.466 kb on - strand at 280.520 kb on - strand at 280.582 kb on - strand at 280.582 kb on - strand at 280.614 kb on + strand at 280.737 kb on - strand, within EX31_RS01255 at 280.784 kb on - strand, within EX31_RS01255 at 280.960 kb on - strand, within EX31_RS01255 at 281.007 kb on - strand, within EX31_RS01255 at 281.007 kb on - strand, within EX31_RS01255 at 281.120 kb on + strand, within EX31_RS01255 at 281.192 kb on + strand at 281.192 kb on + strand at 281.212 kb on + strand at 281.212 kb on + strand at 281.317 kb on - strand at 281.317 kb on - strand at 281.489 kb on + strand, within EX31_RS01260 at 281.572 kb on - strand, within EX31_RS01260 at 281.617 kb on + strand, within EX31_RS01260 at 281.714 kb on - strand at 281.754 kb on - strand at 281.804 kb on + strand at 281.805 kb on - strand at 281.805 kb on - strand at 281.805 kb on - strand at 281.805 kb on - strand at 281.816 kb on + strand at 281.817 kb on - strand at 281.817 kb on - strand at 281.817 kb on - strand at 281.817 kb on - strand at 281.817 kb on - strand at 281.935 kb on + strand at 281.935 kb on + strand at 281.935 kb on + strand at 281.935 kb on + strand at 281.936 kb on - strand at 281.936 kb on - strand at 282.145 kb on + strand, within EX31_RS01265 at 282.145 kb on + strand, within EX31_RS01265 at 282.146 kb on - strand, within EX31_RS01265 at 282.146 kb on - strand, within EX31_RS01265 at 282.146 kb on - strand, within EX31_RS01265 at 282.246 kb on + strand, within EX31_RS01265 at 282.247 kb on - strand, within EX31_RS01265 at 282.247 kb on - strand, within EX31_RS01265
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 279,619 + EX31_RS01250 0.38 +1.3 279,620 - EX31_RS01250 0.38 -0.4 279,671 + EX31_RS01250 0.42 +0.7 279,780 + EX31_RS01250 0.49 +0.3 279,893 + EX31_RS01250 0.58 -1.9 279,893 + EX31_RS01250 0.58 +1.8 279,894 - EX31_RS01250 0.58 +0.5 280,225 + EX31_RS01250 0.81 -0.9 280,225 + EX31_RS01250 0.81 +1.5 280,225 + EX31_RS01250 0.81 +0.7 280,225 + EX31_RS01250 0.81 +1.3 280,226 - EX31_RS01250 0.81 -0.2 280,226 - EX31_RS01250 0.81 +0.6 280,226 - EX31_RS01250 0.81 -0.4 280,226 - EX31_RS01250 0.81 +0.6 280,226 - EX31_RS01250 0.81 -0.6 280,226 - EX31_RS01250 0.81 +2.0 280,294 + EX31_RS01250 0.86 -0.3 280,327 + EX31_RS01250 0.89 -0.6 280,327 + EX31_RS01250 0.89 +0.0 280,328 - EX31_RS01250 0.89 -0.5 280,433 - +0.5 280,433 - +0.1 280,466 - +2.2 280,520 - -0.4 280,582 - +0.4 280,582 - -0.8 280,614 + -0.2 280,737 - EX31_RS01255 0.19 +0.4 280,784 - EX31_RS01255 0.26 -0.2 280,960 - EX31_RS01255 0.54 +0.6 281,007 - EX31_RS01255 0.61 -1.7 281,007 - EX31_RS01255 0.61 -1.3 281,120 + EX31_RS01255 0.79 +0.8 281,192 + -0.9 281,192 + -0.2 281,212 + -0.2 281,212 + +1.4 281,317 - +0.7 281,317 - -0.4 281,489 + EX31_RS01260 0.47 -0.8 281,572 - EX31_RS01260 0.68 -0.8 281,617 + EX31_RS01260 0.79 +0.7 281,714 - -0.7 281,754 - -0.2 281,804 + -0.6 281,805 - -0.2 281,805 - -0.9 281,805 - -2.7 281,805 - -0.5 281,816 + -0.2 281,817 - +0.4 281,817 - +0.2 281,817 - +0.8 281,817 - -1.4 281,817 - +0.4 281,935 + +0.6 281,935 + -1.4 281,935 + +0.5 281,935 + +1.2 281,936 - -2.0 281,936 - +0.2 282,145 + EX31_RS01265 0.53 -0.4 282,145 + EX31_RS01265 0.53 +0.8 282,146 - EX31_RS01265 0.54 +1.8 282,146 - EX31_RS01265 0.54 +1.2 282,146 - EX31_RS01265 0.54 +0.2 282,246 + EX31_RS01265 0.75 -0.5 282,247 - EX31_RS01265 0.76 +1.2 282,247 - EX31_RS01265 0.76 +0.4
Or see this region's nucleotide sequence