Experiment: L-Glutamine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01230 and EX31_RS01235 are separated by 44 nucleotides EX31_RS01235 and EX31_RS01240 are separated by 229 nucleotides EX31_RS01240 and EX31_RS01245 are separated by 70 nucleotides
EX31_RS01230: EX31_RS01230 - arsenic transporter, at 273,319 to 274,608
_RS01230
EX31_RS01235: EX31_RS01235 - metalloregulator ArsR/SmtB family transcription factor, at 274,653 to 274,973
_RS01235
EX31_RS01240: EX31_RS01240 - glycoside hydrolase family 10 protein, at 275,203 to 276,588
_RS01240
EX31_RS01245: EX31_RS01245 - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 276,659 to 278,869
_RS01245
Position (kb) 275
276
277 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 274.314 kb on + strand, within EX31_RS01230 at 274.314 kb on + strand, within EX31_RS01230 at 274.324 kb on - strand, within EX31_RS01230 at 274.324 kb on - strand, within EX31_RS01230 at 274.327 kb on + strand, within EX31_RS01230 at 274.328 kb on - strand, within EX31_RS01230 at 274.328 kb on - strand, within EX31_RS01230 at 274.414 kb on - strand, within EX31_RS01230 at 274.454 kb on - strand, within EX31_RS01230 at 274.454 kb on - strand, within EX31_RS01230 at 274.593 kb on + strand at 274.818 kb on + strand, within EX31_RS01235 at 274.819 kb on - strand, within EX31_RS01235 at 274.819 kb on - strand, within EX31_RS01235 at 274.819 kb on - strand, within EX31_RS01235 at 274.819 kb on - strand, within EX31_RS01235 at 274.819 kb on - strand, within EX31_RS01235 at 274.875 kb on - strand, within EX31_RS01235 at 274.974 kb on - strand at 274.974 kb on - strand at 275.063 kb on + strand at 275.171 kb on - strand at 275.250 kb on + strand at 275.327 kb on + strand at 275.484 kb on + strand, within EX31_RS01240 at 275.562 kb on + strand, within EX31_RS01240 at 275.562 kb on + strand, within EX31_RS01240 at 275.563 kb on - strand, within EX31_RS01240 at 275.580 kb on + strand, within EX31_RS01240 at 275.597 kb on + strand, within EX31_RS01240 at 275.597 kb on + strand, within EX31_RS01240 at 275.650 kb on - strand, within EX31_RS01240 at 275.700 kb on + strand, within EX31_RS01240 at 275.701 kb on - strand, within EX31_RS01240 at 275.748 kb on + strand, within EX31_RS01240 at 275.892 kb on + strand, within EX31_RS01240 at 275.977 kb on + strand, within EX31_RS01240 at 276.034 kb on + strand, within EX31_RS01240 at 276.035 kb on - strand, within EX31_RS01240 at 276.035 kb on - strand, within EX31_RS01240 at 276.113 kb on + strand, within EX31_RS01240 at 276.113 kb on + strand, within EX31_RS01240 at 276.229 kb on + strand, within EX31_RS01240 at 276.271 kb on + strand, within EX31_RS01240 at 276.271 kb on + strand, within EX31_RS01240 at 276.271 kb on + strand, within EX31_RS01240 at 276.272 kb on - strand, within EX31_RS01240 at 276.273 kb on + strand, within EX31_RS01240 at 276.273 kb on + strand, within EX31_RS01240 at 276.273 kb on + strand, within EX31_RS01240 at 276.273 kb on + strand, within EX31_RS01240 at 276.273 kb on + strand, within EX31_RS01240 at 276.273 kb on + strand, within EX31_RS01240 at 276.274 kb on - strand, within EX31_RS01240 at 276.366 kb on + strand, within EX31_RS01240 at 276.366 kb on + strand, within EX31_RS01240 at 276.376 kb on + strand, within EX31_RS01240 at 276.394 kb on + strand, within EX31_RS01240 at 276.394 kb on + strand, within EX31_RS01240 at 276.394 kb on + strand, within EX31_RS01240 at 276.394 kb on + strand, within EX31_RS01240 at 276.396 kb on + strand, within EX31_RS01240 at 276.591 kb on + strand at 276.599 kb on + strand at 276.599 kb on + strand at 276.599 kb on + strand at 276.657 kb on + strand at 276.714 kb on + strand at 276.732 kb on + strand at 276.735 kb on + strand at 276.735 kb on + strand at 276.735 kb on + strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.795 kb on + strand at 276.896 kb on + strand, within EX31_RS01245 at 276.973 kb on + strand, within EX31_RS01245 at 276.973 kb on + strand, within EX31_RS01245 at 276.973 kb on + strand, within EX31_RS01245 at 277.014 kb on + strand, within EX31_RS01245 at 277.014 kb on + strand, within EX31_RS01245 at 277.014 kb on + strand, within EX31_RS01245 at 277.110 kb on + strand, within EX31_RS01245 at 277.185 kb on - strand, within EX31_RS01245 at 277.201 kb on + strand, within EX31_RS01245 at 277.229 kb on - strand, within EX31_RS01245 at 277.269 kb on + strand, within EX31_RS01245 at 277.269 kb on + strand, within EX31_RS01245 at 277.270 kb on - strand, within EX31_RS01245 at 277.315 kb on + strand, within EX31_RS01245 at 277.316 kb on - strand, within EX31_RS01245 at 277.316 kb on - strand, within EX31_RS01245 at 277.316 kb on - strand, within EX31_RS01245 at 277.316 kb on - strand, within EX31_RS01245 at 277.317 kb on + strand, within EX31_RS01245 at 277.354 kb on - strand, within EX31_RS01245 at 277.426 kb on + strand, within EX31_RS01245 at 277.427 kb on - strand, within EX31_RS01245 at 277.428 kb on + strand, within EX31_RS01245 at 277.428 kb on + strand, within EX31_RS01245 at 277.524 kb on + strand, within EX31_RS01245
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; shaking remove 274,314 + EX31_RS01230 0.77 +0.2 274,314 + EX31_RS01230 0.77 -0.4 274,324 - EX31_RS01230 0.78 +0.6 274,324 - EX31_RS01230 0.78 -1.3 274,327 + EX31_RS01230 0.78 +0.2 274,328 - EX31_RS01230 0.78 +2.4 274,328 - EX31_RS01230 0.78 -1.6 274,414 - EX31_RS01230 0.85 -1.1 274,454 - EX31_RS01230 0.88 +0.3 274,454 - EX31_RS01230 0.88 -0.5 274,593 + -1.2 274,818 + EX31_RS01235 0.51 -0.0 274,819 - EX31_RS01235 0.52 -0.4 274,819 - EX31_RS01235 0.52 -0.9 274,819 - EX31_RS01235 0.52 -1.9 274,819 - EX31_RS01235 0.52 -1.3 274,819 - EX31_RS01235 0.52 +1.4 274,875 - EX31_RS01235 0.69 +3.2 274,974 - -0.4 274,974 - +0.5 275,063 + -1.0 275,171 - +0.6 275,250 + +0.3 275,327 + +1.4 275,484 + EX31_RS01240 0.20 +0.3 275,562 + EX31_RS01240 0.26 +1.6 275,562 + EX31_RS01240 0.26 +1.2 275,563 - EX31_RS01240 0.26 -0.2 275,580 + EX31_RS01240 0.27 +0.6 275,597 + EX31_RS01240 0.28 -2.1 275,597 + EX31_RS01240 0.28 +0.9 275,650 - EX31_RS01240 0.32 +1.7 275,700 + EX31_RS01240 0.36 +1.2 275,701 - EX31_RS01240 0.36 +0.8 275,748 + EX31_RS01240 0.39 -1.0 275,892 + EX31_RS01240 0.50 -0.3 275,977 + EX31_RS01240 0.56 +2.4 276,034 + EX31_RS01240 0.60 +0.7 276,035 - EX31_RS01240 0.60 +0.4 276,035 - EX31_RS01240 0.60 -1.0 276,113 + EX31_RS01240 0.66 +0.1 276,113 + EX31_RS01240 0.66 -0.0 276,229 + EX31_RS01240 0.74 -0.2 276,271 + EX31_RS01240 0.77 -0.1 276,271 + EX31_RS01240 0.77 -1.4 276,271 + EX31_RS01240 0.77 -0.2 276,272 - EX31_RS01240 0.77 -1.2 276,273 + EX31_RS01240 0.77 -1.4 276,273 + EX31_RS01240 0.77 +1.5 276,273 + EX31_RS01240 0.77 -0.0 276,273 + EX31_RS01240 0.77 +0.1 276,273 + EX31_RS01240 0.77 +0.9 276,273 + EX31_RS01240 0.77 +0.6 276,274 - EX31_RS01240 0.77 +1.6 276,366 + EX31_RS01240 0.84 +1.2 276,366 + EX31_RS01240 0.84 +0.0 276,376 + EX31_RS01240 0.85 +1.5 276,394 + EX31_RS01240 0.86 +0.4 276,394 + EX31_RS01240 0.86 +0.7 276,394 + EX31_RS01240 0.86 +1.2 276,394 + EX31_RS01240 0.86 -1.4 276,396 + EX31_RS01240 0.86 -0.8 276,591 + +0.5 276,599 + -0.1 276,599 + -0.9 276,599 + -2.1 276,657 + -0.3 276,714 + -0.2 276,732 + +0.2 276,735 + +1.9 276,735 + +1.8 276,735 + +2.9 276,736 - -0.3 276,736 - -0.3 276,736 - +0.2 276,736 - +0.2 276,736 - -1.2 276,795 + +1.4 276,896 + EX31_RS01245 0.11 -0.4 276,973 + EX31_RS01245 0.14 +0.3 276,973 + EX31_RS01245 0.14 +0.4 276,973 + EX31_RS01245 0.14 -0.0 277,014 + EX31_RS01245 0.16 +0.2 277,014 + EX31_RS01245 0.16 -0.2 277,014 + EX31_RS01245 0.16 +3.0 277,110 + EX31_RS01245 0.20 +0.8 277,185 - EX31_RS01245 0.24 -1.2 277,201 + EX31_RS01245 0.25 -0.6 277,229 - EX31_RS01245 0.26 +0.2 277,269 + EX31_RS01245 0.28 +1.4 277,269 + EX31_RS01245 0.28 -0.7 277,270 - EX31_RS01245 0.28 +0.3 277,315 + EX31_RS01245 0.30 -0.2 277,316 - EX31_RS01245 0.30 -0.5 277,316 - EX31_RS01245 0.30 +1.2 277,316 - EX31_RS01245 0.30 -1.1 277,316 - EX31_RS01245 0.30 -0.7 277,317 + EX31_RS01245 0.30 -0.7 277,354 - EX31_RS01245 0.31 -0.1 277,426 + EX31_RS01245 0.35 +2.2 277,427 - EX31_RS01245 0.35 +0.2 277,428 + EX31_RS01245 0.35 +0.8 277,428 + EX31_RS01245 0.35 -1.0 277,524 + EX31_RS01245 0.39 +0.2
Or see this region's nucleotide sequence