Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25230 and CA265_RS25235 are separated by 173 nucleotides CA265_RS25235 and CA265_RS25240 are separated by 108 nucleotides CA265_RS25240 and CA265_RS25245 are separated by 63 nucleotides
CA265_RS25230: CA265_RS25230 - LPS export ABC transporter ATP-binding protein, at 6,141,291 to 6,142,031
_RS25230
CA265_RS25235: CA265_RS25235 - hypothetical protein, at 6,142,205 to 6,143,722
_RS25235
CA265_RS25240: CA265_RS25240 - hypothetical protein, at 6,143,831 to 6,144,238
_RS25240
CA265_RS25245: CA265_RS25245 - hypothetical protein, at 6,144,302 to 6,144,865
_RS25245
Position (kb)
6142
6143
6144 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6141.290 kb on + strand at 6142.032 kb on + strand at 6142.038 kb on - strand at 6142.153 kb on + strand at 6142.156 kb on - strand at 6142.309 kb on + strand at 6142.417 kb on + strand, within CA265_RS25235 at 6142.430 kb on + strand, within CA265_RS25235 at 6142.462 kb on + strand, within CA265_RS25235 at 6142.519 kb on + strand, within CA265_RS25235 at 6142.520 kb on - strand, within CA265_RS25235 at 6142.586 kb on - strand, within CA265_RS25235 at 6142.599 kb on - strand, within CA265_RS25235 at 6142.670 kb on - strand, within CA265_RS25235 at 6142.745 kb on + strand, within CA265_RS25235 at 6142.748 kb on + strand, within CA265_RS25235 at 6142.873 kb on - strand, within CA265_RS25235 at 6142.944 kb on - strand, within CA265_RS25235 at 6142.946 kb on - strand, within CA265_RS25235 at 6143.016 kb on - strand, within CA265_RS25235 at 6143.016 kb on - strand, within CA265_RS25235 at 6143.031 kb on - strand, within CA265_RS25235 at 6143.138 kb on - strand, within CA265_RS25235 at 6143.213 kb on - strand, within CA265_RS25235 at 6143.237 kb on - strand, within CA265_RS25235 at 6143.237 kb on - strand, within CA265_RS25235 at 6143.237 kb on - strand, within CA265_RS25235 at 6143.237 kb on - strand, within CA265_RS25235 at 6143.261 kb on - strand, within CA265_RS25235 at 6143.312 kb on - strand at 6143.335 kb on - strand, within CA265_RS25235 at 6143.402 kb on + strand, within CA265_RS25235 at 6143.405 kb on - strand, within CA265_RS25235 at 6143.500 kb on - strand, within CA265_RS25235 at 6143.500 kb on - strand, within CA265_RS25235 at 6143.504 kb on - strand, within CA265_RS25235 at 6143.581 kb on + strand at 6143.581 kb on + strand at 6143.587 kb on - strand at 6143.606 kb on - strand at 6143.612 kb on + strand at 6143.636 kb on - strand at 6143.685 kb on - strand at 6143.696 kb on - strand at 6143.806 kb on - strand at 6143.813 kb on - strand at 6143.874 kb on + strand, within CA265_RS25240 at 6143.874 kb on + strand, within CA265_RS25240 at 6143.876 kb on + strand, within CA265_RS25240 at 6143.885 kb on - strand, within CA265_RS25240 at 6143.925 kb on - strand, within CA265_RS25240 at 6143.925 kb on - strand, within CA265_RS25240 at 6143.926 kb on + strand, within CA265_RS25240 at 6143.927 kb on - strand, within CA265_RS25240 at 6143.932 kb on - strand, within CA265_RS25240 at 6143.963 kb on - strand, within CA265_RS25240 at 6144.016 kb on + strand, within CA265_RS25240 at 6144.076 kb on + strand, within CA265_RS25240 at 6144.172 kb on - strand, within CA265_RS25240 at 6144.236 kb on + strand at 6144.314 kb on - strand at 6144.318 kb on + strand at 6144.324 kb on - strand at 6144.392 kb on + strand, within CA265_RS25245 at 6144.407 kb on + strand, within CA265_RS25245 at 6144.408 kb on - strand, within CA265_RS25245 at 6144.429 kb on + strand, within CA265_RS25245 at 6144.430 kb on - strand, within CA265_RS25245 at 6144.430 kb on - strand, within CA265_RS25245 at 6144.451 kb on + strand, within CA265_RS25245 at 6144.452 kb on - strand, within CA265_RS25245 at 6144.461 kb on - strand, within CA265_RS25245 at 6144.461 kb on - strand, within CA265_RS25245 at 6144.515 kb on + strand, within CA265_RS25245 at 6144.516 kb on - strand, within CA265_RS25245 at 6144.529 kb on - strand, within CA265_RS25245 at 6144.586 kb on - strand, within CA265_RS25245 at 6144.607 kb on + strand, within CA265_RS25245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 6,141,290 + +0.5 6,142,032 + -1.4 6,142,038 - -0.5 6,142,153 + +0.1 6,142,156 - -0.3 6,142,309 + +2.1 6,142,417 + CA265_RS25235 0.14 +1.4 6,142,430 + CA265_RS25235 0.15 +1.3 6,142,462 + CA265_RS25235 0.17 +1.9 6,142,519 + CA265_RS25235 0.21 +1.5 6,142,520 - CA265_RS25235 0.21 +1.1 6,142,586 - CA265_RS25235 0.25 +2.2 6,142,599 - CA265_RS25235 0.26 +2.1 6,142,670 - CA265_RS25235 0.31 +2.0 6,142,745 + CA265_RS25235 0.36 +2.5 6,142,748 + CA265_RS25235 0.36 +1.6 6,142,873 - CA265_RS25235 0.44 +1.8 6,142,944 - CA265_RS25235 0.49 +1.6 6,142,946 - CA265_RS25235 0.49 +1.1 6,143,016 - CA265_RS25235 0.53 +2.2 6,143,016 - CA265_RS25235 0.53 +1.6 6,143,031 - CA265_RS25235 0.54 +1.7 6,143,138 - CA265_RS25235 0.61 +0.4 6,143,213 - CA265_RS25235 0.66 +1.7 6,143,237 - CA265_RS25235 0.68 +0.9 6,143,237 - CA265_RS25235 0.68 +1.7 6,143,237 - CA265_RS25235 0.68 +0.8 6,143,237 - CA265_RS25235 0.68 +2.2 6,143,261 - CA265_RS25235 0.70 -2.0 6,143,312 - +2.6 6,143,335 - CA265_RS25235 0.74 +2.6 6,143,402 + CA265_RS25235 0.79 +0.5 6,143,405 - CA265_RS25235 0.79 +1.0 6,143,500 - CA265_RS25235 0.85 +2.3 6,143,500 - CA265_RS25235 0.85 +1.9 6,143,504 - CA265_RS25235 0.86 +2.1 6,143,581 + -0.2 6,143,581 + +3.7 6,143,587 - +1.7 6,143,606 - +1.6 6,143,612 + +1.6 6,143,636 - +1.4 6,143,685 - +2.2 6,143,696 - +1.0 6,143,806 - -0.2 6,143,813 - +1.0 6,143,874 + CA265_RS25240 0.11 -0.1 6,143,874 + CA265_RS25240 0.11 -0.7 6,143,876 + CA265_RS25240 0.11 -0.4 6,143,885 - CA265_RS25240 0.13 -0.2 6,143,925 - CA265_RS25240 0.23 -1.7 6,143,925 - CA265_RS25240 0.23 -0.3 6,143,926 + CA265_RS25240 0.23 -0.1 6,143,927 - CA265_RS25240 0.24 -0.2 6,143,932 - CA265_RS25240 0.25 +0.3 6,143,963 - CA265_RS25240 0.32 +1.0 6,144,016 + CA265_RS25240 0.45 -0.6 6,144,076 + CA265_RS25240 0.60 +0.3 6,144,172 - CA265_RS25240 0.84 +1.4 6,144,236 + -0.6 6,144,314 - +0.5 6,144,318 + -0.1 6,144,324 - +0.1 6,144,392 + CA265_RS25245 0.16 +1.4 6,144,407 + CA265_RS25245 0.19 +1.7 6,144,408 - CA265_RS25245 0.19 -0.5 6,144,429 + CA265_RS25245 0.23 -0.1 6,144,430 - CA265_RS25245 0.23 -0.3 6,144,430 - CA265_RS25245 0.23 +1.5 6,144,451 + CA265_RS25245 0.26 +0.6 6,144,452 - CA265_RS25245 0.27 +0.2 6,144,461 - CA265_RS25245 0.28 -1.9 6,144,461 - CA265_RS25245 0.28 +0.7 6,144,515 + CA265_RS25245 0.38 -0.1 6,144,516 - CA265_RS25245 0.38 -0.3 6,144,529 - CA265_RS25245 0.40 -0.1 6,144,586 - CA265_RS25245 0.50 -0.6 6,144,607 + CA265_RS25245 0.54 +0.1
Or see this region's nucleotide sequence