Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15985 and CA265_RS15990 are separated by 248 nucleotides CA265_RS15990 and CA265_RS15995 are separated by 301 nucleotides
CA265_RS15985: CA265_RS15985 - mechanosensitive ion channel protein MscS, at 4,011,970 to 4,013,244
_RS15985
CA265_RS15990: CA265_RS15990 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 4,013,493 to 4,015,469
_RS15990
CA265_RS15995: CA265_RS15995 - hypothetical protein, at 4,015,771 to 4,016,382
_RS15995
Position (kb)
4013
4014
4015
4016 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4012.509 kb on + strand, within CA265_RS15985 at 4012.597 kb on - strand, within CA265_RS15985 at 4012.696 kb on - strand, within CA265_RS15985 at 4012.721 kb on + strand, within CA265_RS15985 at 4012.722 kb on - strand, within CA265_RS15985 at 4012.724 kb on - strand, within CA265_RS15985 at 4012.775 kb on + strand, within CA265_RS15985 at 4012.776 kb on - strand, within CA265_RS15985 at 4012.776 kb on - strand, within CA265_RS15985 at 4012.776 kb on - strand, within CA265_RS15985 at 4012.776 kb on - strand, within CA265_RS15985 at 4012.776 kb on - strand, within CA265_RS15985 at 4012.826 kb on + strand, within CA265_RS15985 at 4012.918 kb on + strand, within CA265_RS15985 at 4012.918 kb on + strand, within CA265_RS15985 at 4012.919 kb on - strand, within CA265_RS15985 at 4012.921 kb on + strand, within CA265_RS15985 at 4012.921 kb on + strand, within CA265_RS15985 at 4012.975 kb on - strand, within CA265_RS15985 at 4012.976 kb on + strand, within CA265_RS15985 at 4012.977 kb on - strand, within CA265_RS15985 at 4012.985 kb on + strand, within CA265_RS15985 at 4012.985 kb on + strand, within CA265_RS15985 at 4012.986 kb on - strand, within CA265_RS15985 at 4013.105 kb on - strand, within CA265_RS15985 at 4013.123 kb on - strand at 4013.136 kb on + strand at 4013.137 kb on - strand at 4013.240 kb on + strand at 4013.357 kb on - strand at 4013.359 kb on + strand at 4013.365 kb on + strand at 4013.365 kb on + strand at 4013.368 kb on - strand at 4013.424 kb on + strand at 4013.425 kb on - strand at 4013.455 kb on + strand at 4013.456 kb on - strand at 4013.456 kb on - strand at 4015.504 kb on - strand at 4015.519 kb on + strand at 4015.524 kb on - strand at 4015.540 kb on - strand at 4015.583 kb on - strand at 4015.583 kb on - strand at 4015.672 kb on + strand at 4015.718 kb on - strand at 4015.719 kb on + strand at 4015.719 kb on + strand at 4015.724 kb on + strand at 4015.773 kb on - strand at 4015.773 kb on - strand at 4015.773 kb on - strand at 4015.774 kb on + strand at 4015.775 kb on - strand at 4015.800 kb on + strand at 4015.800 kb on + strand at 4015.800 kb on + strand at 4015.813 kb on - strand at 4015.813 kb on - strand at 4015.876 kb on + strand, within CA265_RS15995 at 4015.885 kb on + strand, within CA265_RS15995 at 4015.886 kb on - strand, within CA265_RS15995 at 4015.886 kb on - strand, within CA265_RS15995 at 4016.019 kb on + strand, within CA265_RS15995 at 4016.025 kb on + strand, within CA265_RS15995 at 4016.032 kb on - strand, within CA265_RS15995 at 4016.035 kb on + strand, within CA265_RS15995 at 4016.036 kb on - strand, within CA265_RS15995 at 4016.036 kb on - strand, within CA265_RS15995 at 4016.096 kb on + strand, within CA265_RS15995 at 4016.119 kb on - strand, within CA265_RS15995 at 4016.120 kb on + strand, within CA265_RS15995 at 4016.121 kb on - strand, within CA265_RS15995 at 4016.146 kb on + strand, within CA265_RS15995 at 4016.186 kb on + strand, within CA265_RS15995 at 4016.283 kb on + strand, within CA265_RS15995 at 4016.324 kb on - strand at 4016.331 kb on - strand at 4016.363 kb on + strand at 4016.366 kb on + strand at 4016.367 kb on - strand at 4016.383 kb on + strand at 4016.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 4,012,509 + CA265_RS15985 0.42 -0.6 4,012,597 - CA265_RS15985 0.49 -0.0 4,012,696 - CA265_RS15985 0.57 -0.8 4,012,721 + CA265_RS15985 0.59 -0.3 4,012,722 - CA265_RS15985 0.59 -0.9 4,012,724 - CA265_RS15985 0.59 +0.5 4,012,775 + CA265_RS15985 0.63 -1.1 4,012,776 - CA265_RS15985 0.63 +1.9 4,012,776 - CA265_RS15985 0.63 -0.4 4,012,776 - CA265_RS15985 0.63 +1.2 4,012,776 - CA265_RS15985 0.63 -0.3 4,012,776 - CA265_RS15985 0.63 +1.7 4,012,826 + CA265_RS15985 0.67 -0.4 4,012,918 + CA265_RS15985 0.74 -0.4 4,012,918 + CA265_RS15985 0.74 -0.3 4,012,919 - CA265_RS15985 0.74 -1.1 4,012,921 + CA265_RS15985 0.75 -0.7 4,012,921 + CA265_RS15985 0.75 +0.5 4,012,975 - CA265_RS15985 0.79 -0.0 4,012,976 + CA265_RS15985 0.79 -2.0 4,012,977 - CA265_RS15985 0.79 -0.5 4,012,985 + CA265_RS15985 0.80 +0.0 4,012,985 + CA265_RS15985 0.80 +0.1 4,012,986 - CA265_RS15985 0.80 -0.2 4,013,105 - CA265_RS15985 0.89 -1.6 4,013,123 - +0.9 4,013,136 + +0.5 4,013,137 - +0.6 4,013,240 + +0.1 4,013,357 - -0.1 4,013,359 + +0.4 4,013,365 + +0.1 4,013,365 + +0.6 4,013,368 - +0.0 4,013,424 + +1.3 4,013,425 - +0.8 4,013,455 + +0.3 4,013,456 - +1.9 4,013,456 - +0.9 4,015,504 - +1.6 4,015,519 + -0.7 4,015,524 - +0.0 4,015,540 - +0.0 4,015,583 - +0.1 4,015,583 - -0.1 4,015,672 + +0.2 4,015,718 - -0.1 4,015,719 + +1.0 4,015,719 + +1.9 4,015,724 + +0.1 4,015,773 - -0.3 4,015,773 - +0.5 4,015,773 - +0.4 4,015,774 + +0.6 4,015,775 - +0.2 4,015,800 + -0.9 4,015,800 + +0.1 4,015,800 + +1.4 4,015,813 - -0.1 4,015,813 - -0.2 4,015,876 + CA265_RS15995 0.17 +0.1 4,015,885 + CA265_RS15995 0.19 -0.7 4,015,886 - CA265_RS15995 0.19 +0.3 4,015,886 - CA265_RS15995 0.19 -0.0 4,016,019 + CA265_RS15995 0.41 +1.4 4,016,025 + CA265_RS15995 0.42 -0.1 4,016,032 - CA265_RS15995 0.43 -0.4 4,016,035 + CA265_RS15995 0.43 +0.8 4,016,036 - CA265_RS15995 0.43 -0.0 4,016,036 - CA265_RS15995 0.43 -0.5 4,016,096 + CA265_RS15995 0.53 +1.2 4,016,119 - CA265_RS15995 0.57 -0.3 4,016,120 + CA265_RS15995 0.57 +0.3 4,016,121 - CA265_RS15995 0.57 +0.8 4,016,146 + CA265_RS15995 0.61 +0.4 4,016,186 + CA265_RS15995 0.68 +0.5 4,016,283 + CA265_RS15995 0.84 -0.6 4,016,324 - +0.4 4,016,331 - +0.9 4,016,363 + +0.0 4,016,366 + +1.7 4,016,367 - +0.2 4,016,383 + -0.5 4,016,418 - +0.6
Or see this region's nucleotide sequence