Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13025 and CA265_RS13030 are separated by 258 nucleotides CA265_RS13030 and CA265_RS13035 are separated by 1 nucleotides
CA265_RS13025: CA265_RS13025 - transmembrane glycosyltransferase, at 3,242,031 to 3,243,131
_RS13025
CA265_RS13030: CA265_RS13030 - tRNA guanosine(34) transglycosylase Tgt, at 3,243,390 to 3,244,520
_RS13030
CA265_RS13035: CA265_RS13035 - permease, at 3,244,522 to 3,245,625
_RS13035
Position (kb)
3243
3244
3245 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3242.427 kb on - strand, within CA265_RS13025 at 3242.436 kb on - strand, within CA265_RS13025 at 3242.458 kb on - strand, within CA265_RS13025 at 3242.480 kb on + strand, within CA265_RS13025 at 3242.505 kb on - strand, within CA265_RS13025 at 3242.555 kb on + strand, within CA265_RS13025 at 3242.556 kb on - strand, within CA265_RS13025 at 3242.556 kb on - strand, within CA265_RS13025 at 3242.557 kb on + strand, within CA265_RS13025 at 3242.557 kb on + strand, within CA265_RS13025 at 3242.557 kb on + strand, within CA265_RS13025 at 3242.557 kb on + strand, within CA265_RS13025 at 3242.558 kb on - strand, within CA265_RS13025 at 3242.654 kb on - strand, within CA265_RS13025 at 3242.705 kb on + strand, within CA265_RS13025 at 3242.768 kb on - strand, within CA265_RS13025 at 3242.841 kb on - strand, within CA265_RS13025 at 3242.844 kb on + strand, within CA265_RS13025 at 3242.894 kb on + strand, within CA265_RS13025 at 3242.953 kb on - strand, within CA265_RS13025 at 3242.955 kb on + strand, within CA265_RS13025 at 3242.960 kb on + strand, within CA265_RS13025 at 3242.961 kb on - strand, within CA265_RS13025 at 3242.969 kb on - strand, within CA265_RS13025 at 3243.009 kb on + strand, within CA265_RS13025 at 3243.010 kb on - strand, within CA265_RS13025 at 3243.015 kb on + strand, within CA265_RS13025 at 3243.016 kb on - strand, within CA265_RS13025 at 3243.054 kb on + strand at 3243.165 kb on - strand at 3243.170 kb on - strand at 3243.202 kb on - strand at 3243.238 kb on + strand at 3243.238 kb on + strand at 3243.291 kb on - strand at 3243.293 kb on - strand at 3243.327 kb on + strand at 3243.379 kb on + strand at 3243.403 kb on + strand at 3243.425 kb on + strand at 3243.543 kb on - strand, within CA265_RS13030 at 3243.543 kb on - strand, within CA265_RS13030 at 3243.544 kb on - strand, within CA265_RS13030 at 3243.562 kb on + strand, within CA265_RS13030 at 3243.573 kb on + strand, within CA265_RS13030 at 3243.574 kb on - strand, within CA265_RS13030 at 3243.581 kb on - strand, within CA265_RS13030 at 3243.684 kb on + strand, within CA265_RS13030 at 3243.725 kb on + strand, within CA265_RS13030 at 3243.775 kb on - strand, within CA265_RS13030 at 3243.784 kb on - strand, within CA265_RS13030 at 3243.833 kb on + strand, within CA265_RS13030 at 3243.852 kb on + strand, within CA265_RS13030 at 3243.859 kb on - strand, within CA265_RS13030 at 3243.859 kb on - strand, within CA265_RS13030 at 3243.859 kb on - strand, within CA265_RS13030 at 3243.859 kb on - strand, within CA265_RS13030 at 3243.980 kb on - strand, within CA265_RS13030 at 3243.980 kb on - strand, within CA265_RS13030 at 3243.982 kb on - strand, within CA265_RS13030 at 3243.992 kb on - strand, within CA265_RS13030 at 3243.992 kb on - strand, within CA265_RS13030 at 3244.014 kb on - strand, within CA265_RS13030 at 3244.083 kb on + strand, within CA265_RS13030 at 3244.084 kb on - strand, within CA265_RS13030 at 3244.260 kb on - strand, within CA265_RS13030 at 3244.262 kb on + strand, within CA265_RS13030 at 3244.350 kb on + strand, within CA265_RS13030 at 3244.423 kb on - strand at 3244.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 3,242,427 - CA265_RS13025 0.36 -4.1 3,242,436 - CA265_RS13025 0.37 -3.0 3,242,458 - CA265_RS13025 0.39 -1.1 3,242,480 + CA265_RS13025 0.41 -2.7 3,242,505 - CA265_RS13025 0.43 -5.0 3,242,555 + CA265_RS13025 0.48 -3.8 3,242,556 - CA265_RS13025 0.48 -2.5 3,242,556 - CA265_RS13025 0.48 -1.1 3,242,557 + CA265_RS13025 0.48 -3.6 3,242,557 + CA265_RS13025 0.48 -2.6 3,242,557 + CA265_RS13025 0.48 -1.1 3,242,557 + CA265_RS13025 0.48 -1.8 3,242,558 - CA265_RS13025 0.48 -0.4 3,242,654 - CA265_RS13025 0.57 -2.3 3,242,705 + CA265_RS13025 0.61 -1.8 3,242,768 - CA265_RS13025 0.67 -1.0 3,242,841 - CA265_RS13025 0.74 -3.7 3,242,844 + CA265_RS13025 0.74 -2.9 3,242,894 + CA265_RS13025 0.78 -1.3 3,242,953 - CA265_RS13025 0.84 -3.0 3,242,955 + CA265_RS13025 0.84 -2.9 3,242,960 + CA265_RS13025 0.84 -2.7 3,242,961 - CA265_RS13025 0.84 -0.6 3,242,969 - CA265_RS13025 0.85 -2.0 3,243,009 + CA265_RS13025 0.89 -2.4 3,243,010 - CA265_RS13025 0.89 -3.1 3,243,015 + CA265_RS13025 0.89 -3.1 3,243,016 - CA265_RS13025 0.89 -2.0 3,243,054 + -2.3 3,243,165 - -3.3 3,243,170 - +1.2 3,243,202 - -0.6 3,243,238 + -4.1 3,243,238 + -3.2 3,243,291 - -0.3 3,243,293 - +0.5 3,243,327 + -2.7 3,243,379 + -2.5 3,243,403 + -1.1 3,243,425 + -2.1 3,243,543 - CA265_RS13030 0.14 -1.8 3,243,543 - CA265_RS13030 0.14 +1.2 3,243,544 - CA265_RS13030 0.14 -2.8 3,243,562 + CA265_RS13030 0.15 -1.4 3,243,573 + CA265_RS13030 0.16 -2.3 3,243,574 - CA265_RS13030 0.16 +0.1 3,243,581 - CA265_RS13030 0.17 -2.6 3,243,684 + CA265_RS13030 0.26 -3.2 3,243,725 + CA265_RS13030 0.30 -2.5 3,243,775 - CA265_RS13030 0.34 -1.1 3,243,784 - CA265_RS13030 0.35 +0.5 3,243,833 + CA265_RS13030 0.39 -3.4 3,243,852 + CA265_RS13030 0.41 -2.2 3,243,859 - CA265_RS13030 0.41 -2.0 3,243,859 - CA265_RS13030 0.41 -1.3 3,243,859 - CA265_RS13030 0.41 -0.8 3,243,859 - CA265_RS13030 0.41 +0.5 3,243,980 - CA265_RS13030 0.52 +0.3 3,243,980 - CA265_RS13030 0.52 -0.5 3,243,982 - CA265_RS13030 0.52 -0.7 3,243,992 - CA265_RS13030 0.53 -1.8 3,243,992 - CA265_RS13030 0.53 -0.1 3,244,014 - CA265_RS13030 0.55 -1.1 3,244,083 + CA265_RS13030 0.61 -1.6 3,244,084 - CA265_RS13030 0.61 -1.9 3,244,260 - CA265_RS13030 0.77 -1.6 3,244,262 + CA265_RS13030 0.77 -2.3 3,244,350 + CA265_RS13030 0.85 -0.1 3,244,423 - -0.9 3,244,518 + -0.4
Or see this region's nucleotide sequence