Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11760 and CA265_RS11765 are separated by 3 nucleotides CA265_RS11765 and CA265_RS11770 are separated by 12 nucleotides CA265_RS11770 and CA265_RS11775 are separated by 10 nucleotides
CA265_RS11760: CA265_RS11760 - mobilization protein, at 2,913,852 to 2,915,810
_RS11760
CA265_RS11765: CA265_RS11765 - conjugative transposon protein TraN, at 2,915,814 to 2,916,659
_RS11765
CA265_RS11770: CA265_RS11770 - hypothetical protein, at 2,916,672 to 2,917,862
_RS11770
CA265_RS11775: CA265_RS11775 - hypothetical protein, at 2,917,873 to 2,918,157
_RS11775
Position (kb)
2916
2917
2918 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2915.741 kb on + strand at 2915.745 kb on + strand at 2915.745 kb on + strand at 2915.751 kb on - strand at 2915.755 kb on + strand at 2915.798 kb on + strand at 2915.812 kb on - strand at 2915.816 kb on - strand at 2915.823 kb on - strand at 2915.829 kb on + strand at 2915.844 kb on + strand at 2915.844 kb on + strand at 2915.845 kb on - strand at 2915.924 kb on + strand, within CA265_RS11765 at 2915.924 kb on + strand, within CA265_RS11765 at 2915.924 kb on + strand, within CA265_RS11765 at 2915.958 kb on + strand, within CA265_RS11765 at 2915.968 kb on + strand, within CA265_RS11765 at 2916.044 kb on + strand, within CA265_RS11765 at 2916.091 kb on + strand, within CA265_RS11765 at 2916.162 kb on - strand, within CA265_RS11765 at 2916.196 kb on - strand, within CA265_RS11765 at 2916.239 kb on + strand, within CA265_RS11765 at 2916.239 kb on + strand, within CA265_RS11765 at 2916.240 kb on - strand, within CA265_RS11765 at 2916.362 kb on - strand, within CA265_RS11765 at 2916.392 kb on + strand, within CA265_RS11765 at 2916.418 kb on - strand, within CA265_RS11765 at 2916.491 kb on + strand, within CA265_RS11765 at 2916.492 kb on - strand, within CA265_RS11765 at 2916.500 kb on - strand, within CA265_RS11765 at 2916.500 kb on - strand, within CA265_RS11765 at 2916.522 kb on - strand, within CA265_RS11765 at 2916.584 kb on + strand at 2916.646 kb on - strand at 2916.719 kb on - strand at 2916.719 kb on - strand at 2916.770 kb on + strand at 2916.771 kb on - strand at 2916.771 kb on - strand at 2916.786 kb on - strand at 2916.820 kb on - strand, within CA265_RS11770 at 2916.905 kb on - strand, within CA265_RS11770 at 2916.905 kb on - strand, within CA265_RS11770 at 2917.067 kb on - strand, within CA265_RS11770 at 2917.068 kb on + strand, within CA265_RS11770 at 2917.130 kb on + strand, within CA265_RS11770 at 2917.274 kb on + strand, within CA265_RS11770 at 2917.276 kb on + strand, within CA265_RS11770 at 2917.277 kb on - strand, within CA265_RS11770 at 2917.311 kb on - strand, within CA265_RS11770 at 2917.313 kb on - strand, within CA265_RS11770 at 2917.466 kb on + strand, within CA265_RS11770 at 2917.467 kb on - strand, within CA265_RS11770 at 2917.469 kb on + strand, within CA265_RS11770 at 2917.470 kb on - strand, within CA265_RS11770 at 2917.477 kb on + strand, within CA265_RS11770 at 2917.516 kb on + strand, within CA265_RS11770 at 2917.544 kb on + strand, within CA265_RS11770 at 2917.576 kb on + strand, within CA265_RS11770 at 2917.576 kb on + strand, within CA265_RS11770 at 2917.580 kb on + strand, within CA265_RS11770 at 2917.658 kb on + strand, within CA265_RS11770 at 2917.754 kb on + strand at 2917.830 kb on - strand at 2917.833 kb on - strand at 2917.833 kb on - strand at 2917.920 kb on + strand, within CA265_RS11775 at 2917.921 kb on - strand, within CA265_RS11775 at 2917.948 kb on + strand, within CA265_RS11775 at 2918.001 kb on + strand, within CA265_RS11775 at 2918.001 kb on + strand, within CA265_RS11775 at 2918.097 kb on + strand, within CA265_RS11775 at 2918.098 kb on - strand, within CA265_RS11775 at 2918.244 kb on - strand at 2918.745 kb on - strand at 2918.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,915,741 + -1.4 2,915,745 + -2.5 2,915,745 + -0.2 2,915,751 - +1.5 2,915,755 + +2.4 2,915,798 + -1.9 2,915,812 - +0.8 2,915,816 - +1.8 2,915,823 - +2.7 2,915,829 + +0.7 2,915,844 + -0.4 2,915,844 + +0.9 2,915,845 - +0.1 2,915,924 + CA265_RS11765 0.13 -0.6 2,915,924 + CA265_RS11765 0.13 -1.7 2,915,924 + CA265_RS11765 0.13 +0.2 2,915,958 + CA265_RS11765 0.17 -0.5 2,915,968 + CA265_RS11765 0.18 +0.1 2,916,044 + CA265_RS11765 0.27 -0.0 2,916,091 + CA265_RS11765 0.33 -1.0 2,916,162 - CA265_RS11765 0.41 +1.1 2,916,196 - CA265_RS11765 0.45 +0.9 2,916,239 + CA265_RS11765 0.50 -0.2 2,916,239 + CA265_RS11765 0.50 +0.0 2,916,240 - CA265_RS11765 0.50 +0.6 2,916,362 - CA265_RS11765 0.65 +1.2 2,916,392 + CA265_RS11765 0.68 -0.5 2,916,418 - CA265_RS11765 0.71 +0.9 2,916,491 + CA265_RS11765 0.80 +0.3 2,916,492 - CA265_RS11765 0.80 +1.1 2,916,500 - CA265_RS11765 0.81 +0.5 2,916,500 - CA265_RS11765 0.81 +0.8 2,916,522 - CA265_RS11765 0.84 +0.1 2,916,584 + +0.2 2,916,646 - +1.0 2,916,719 - +2.1 2,916,719 - +0.9 2,916,770 + -0.7 2,916,771 - -0.5 2,916,771 - -0.4 2,916,786 - -0.7 2,916,820 - CA265_RS11770 0.12 -0.2 2,916,905 - CA265_RS11770 0.20 +0.2 2,916,905 - CA265_RS11770 0.20 -0.0 2,917,067 - CA265_RS11770 0.33 +0.4 2,917,068 + CA265_RS11770 0.33 -0.7 2,917,130 + CA265_RS11770 0.38 -0.2 2,917,274 + CA265_RS11770 0.51 +0.7 2,917,276 + CA265_RS11770 0.51 +0.2 2,917,277 - CA265_RS11770 0.51 +0.5 2,917,311 - CA265_RS11770 0.54 -0.9 2,917,313 - CA265_RS11770 0.54 +2.3 2,917,466 + CA265_RS11770 0.67 -0.8 2,917,467 - CA265_RS11770 0.67 +0.3 2,917,469 + CA265_RS11770 0.67 +0.2 2,917,470 - CA265_RS11770 0.67 -0.5 2,917,477 + CA265_RS11770 0.68 -0.7 2,917,516 + CA265_RS11770 0.71 -0.1 2,917,544 + CA265_RS11770 0.73 -0.0 2,917,576 + CA265_RS11770 0.76 -1.9 2,917,576 + CA265_RS11770 0.76 -0.4 2,917,580 + CA265_RS11770 0.76 +0.3 2,917,658 + CA265_RS11770 0.83 +0.3 2,917,754 + -0.1 2,917,830 - -0.2 2,917,833 - -0.2 2,917,833 - -0.3 2,917,920 + CA265_RS11775 0.16 +0.6 2,917,921 - CA265_RS11775 0.17 +0.8 2,917,948 + CA265_RS11775 0.26 +0.8 2,918,001 + CA265_RS11775 0.45 -0.1 2,918,001 + CA265_RS11775 0.45 -1.1 2,918,097 + CA265_RS11775 0.79 -1.5 2,918,098 - CA265_RS11775 0.79 +0.6 2,918,244 - +0.1 2,918,745 - -0.9 2,918,808 - -0.3
Or see this region's nucleotide sequence