Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11650 and CA265_RS11655 are separated by 125 nucleotides CA265_RS11655 and CA265_RS11660 are separated by 1 nucleotides CA265_RS11660 and CA265_RS11665 are separated by 272 nucleotides
CA265_RS11650: CA265_RS11650 - hypothetical protein, at 2,888,316 to 2,888,924
_RS11650
CA265_RS11655: CA265_RS11655 - hypothetical protein, at 2,889,050 to 2,889,802
_RS11655
CA265_RS11660: CA265_RS11660 - hypothetical protein, at 2,889,804 to 2,892,119
_RS11660
CA265_RS11665: CA265_RS11665 - transketolase, at 2,892,392 to 2,894,812
_RS11665
Position (kb)
2889
2890
2891
2892
2893 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2888.895 kb on + strand at 2888.895 kb on + strand at 2888.897 kb on + strand at 2888.897 kb on + strand at 2888.898 kb on - strand at 2888.898 kb on - strand at 2888.898 kb on - strand at 2888.955 kb on + strand at 2888.969 kb on + strand at 2888.972 kb on - strand at 2888.972 kb on - strand at 2888.972 kb on - strand at 2888.993 kb on + strand at 2889.006 kb on + strand at 2889.012 kb on + strand at 2889.012 kb on + strand at 2889.023 kb on + strand at 2889.027 kb on + strand at 2889.036 kb on + strand at 2889.036 kb on + strand at 2889.136 kb on + strand, within CA265_RS11655 at 2889.136 kb on + strand, within CA265_RS11655 at 2889.137 kb on - strand, within CA265_RS11655 at 2889.140 kb on - strand, within CA265_RS11655 at 2889.140 kb on - strand, within CA265_RS11655 at 2889.199 kb on + strand, within CA265_RS11655 at 2889.200 kb on - strand, within CA265_RS11655 at 2889.220 kb on + strand, within CA265_RS11655 at 2889.220 kb on + strand, within CA265_RS11655 at 2889.273 kb on + strand, within CA265_RS11655 at 2889.273 kb on + strand, within CA265_RS11655 at 2889.274 kb on - strand, within CA265_RS11655 at 2889.295 kb on + strand, within CA265_RS11655 at 2889.302 kb on + strand, within CA265_RS11655 at 2889.379 kb on + strand, within CA265_RS11655 at 2889.500 kb on - strand, within CA265_RS11655 at 2889.653 kb on + strand, within CA265_RS11655 at 2889.656 kb on - strand, within CA265_RS11655 at 2889.656 kb on - strand, within CA265_RS11655 at 2889.662 kb on - strand, within CA265_RS11655 at 2889.668 kb on - strand, within CA265_RS11655 at 2889.679 kb on - strand, within CA265_RS11655 at 2889.706 kb on - strand, within CA265_RS11655 at 2889.731 kb on - strand at 2889.848 kb on + strand at 2889.876 kb on + strand at 2889.881 kb on + strand at 2889.930 kb on - strand at 2890.174 kb on - strand, within CA265_RS11660 at 2890.188 kb on + strand, within CA265_RS11660 at 2890.188 kb on + strand, within CA265_RS11660 at 2890.189 kb on - strand, within CA265_RS11660 at 2890.189 kb on - strand, within CA265_RS11660 at 2890.189 kb on - strand, within CA265_RS11660 at 2890.189 kb on - strand, within CA265_RS11660 at 2890.196 kb on + strand, within CA265_RS11660 at 2890.255 kb on - strand, within CA265_RS11660 at 2890.255 kb on - strand, within CA265_RS11660 at 2890.267 kb on + strand, within CA265_RS11660 at 2890.274 kb on + strand, within CA265_RS11660 at 2890.331 kb on + strand, within CA265_RS11660 at 2890.331 kb on + strand, within CA265_RS11660 at 2890.331 kb on + strand, within CA265_RS11660 at 2890.333 kb on + strand, within CA265_RS11660 at 2890.334 kb on - strand, within CA265_RS11660 at 2890.334 kb on - strand, within CA265_RS11660 at 2890.334 kb on - strand, within CA265_RS11660 at 2890.334 kb on - strand, within CA265_RS11660 at 2890.371 kb on + strand, within CA265_RS11660 at 2890.389 kb on - strand, within CA265_RS11660 at 2890.456 kb on - strand, within CA265_RS11660 at 2890.463 kb on + strand, within CA265_RS11660 at 2890.470 kb on - strand, within CA265_RS11660 at 2890.485 kb on - strand, within CA265_RS11660 at 2890.489 kb on - strand, within CA265_RS11660 at 2890.489 kb on - strand, within CA265_RS11660 at 2890.489 kb on - strand, within CA265_RS11660 at 2890.560 kb on + strand, within CA265_RS11660 at 2890.583 kb on + strand, within CA265_RS11660 at 2890.589 kb on + strand, within CA265_RS11660 at 2890.589 kb on + strand, within CA265_RS11660 at 2890.653 kb on - strand, within CA265_RS11660 at 2890.668 kb on + strand, within CA265_RS11660 at 2890.875 kb on + strand, within CA265_RS11660 at 2890.876 kb on - strand, within CA265_RS11660 at 2890.964 kb on + strand, within CA265_RS11660 at 2890.973 kb on + strand, within CA265_RS11660 at 2890.975 kb on + strand, within CA265_RS11660 at 2890.975 kb on + strand, within CA265_RS11660 at 2890.976 kb on - strand, within CA265_RS11660 at 2890.987 kb on + strand, within CA265_RS11660 at 2891.024 kb on - strand, within CA265_RS11660 at 2891.025 kb on + strand, within CA265_RS11660 at 2891.026 kb on - strand, within CA265_RS11660 at 2891.026 kb on - strand, within CA265_RS11660 at 2891.091 kb on + strand, within CA265_RS11660 at 2891.106 kb on + strand, within CA265_RS11660 at 2891.110 kb on - strand, within CA265_RS11660 at 2891.163 kb on + strand, within CA265_RS11660 at 2891.177 kb on - strand, within CA265_RS11660 at 2891.187 kb on - strand, within CA265_RS11660 at 2891.264 kb on - strand, within CA265_RS11660 at 2891.344 kb on + strand, within CA265_RS11660 at 2891.344 kb on + strand, within CA265_RS11660 at 2891.345 kb on - strand, within CA265_RS11660 at 2891.348 kb on - strand, within CA265_RS11660 at 2891.352 kb on + strand, within CA265_RS11660 at 2891.422 kb on + strand, within CA265_RS11660 at 2891.444 kb on - strand, within CA265_RS11660 at 2891.454 kb on + strand, within CA265_RS11660 at 2891.466 kb on - strand, within CA265_RS11660 at 2891.466 kb on - strand, within CA265_RS11660 at 2891.509 kb on + strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.510 kb on - strand, within CA265_RS11660 at 2891.558 kb on + strand, within CA265_RS11660 at 2891.565 kb on - strand, within CA265_RS11660 at 2891.567 kb on - strand, within CA265_RS11660 at 2891.567 kb on - strand, within CA265_RS11660 at 2891.567 kb on - strand, within CA265_RS11660 at 2891.634 kb on + strand, within CA265_RS11660 at 2891.647 kb on + strand, within CA265_RS11660 at 2891.647 kb on + strand, within CA265_RS11660 at 2891.658 kb on - strand, within CA265_RS11660 at 2891.666 kb on - strand, within CA265_RS11660 at 2891.667 kb on + strand, within CA265_RS11660 at 2891.668 kb on - strand, within CA265_RS11660 at 2891.686 kb on + strand, within CA265_RS11660 at 2891.687 kb on - strand, within CA265_RS11660 at 2891.687 kb on - strand, within CA265_RS11660 at 2891.703 kb on - strand, within CA265_RS11660 at 2891.786 kb on - strand, within CA265_RS11660 at 2891.790 kb on + strand, within CA265_RS11660 at 2891.790 kb on + strand, within CA265_RS11660 at 2891.791 kb on - strand, within CA265_RS11660 at 2891.803 kb on + strand, within CA265_RS11660 at 2891.862 kb on - strand, within CA265_RS11660 at 2891.869 kb on + strand, within CA265_RS11660 at 2891.883 kb on - strand, within CA265_RS11660 at 2892.142 kb on - strand at 2892.207 kb on - strand at 2892.214 kb on - strand at 2892.269 kb on - strand at 2892.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,888,895 + +0.8 2,888,895 + +0.7 2,888,897 + -0.8 2,888,897 + +0.4 2,888,898 - +0.5 2,888,898 - -0.3 2,888,898 - -0.7 2,888,955 + -0.1 2,888,969 + -1.1 2,888,972 - +1.6 2,888,972 - -0.7 2,888,972 - +1.5 2,888,993 + +0.7 2,889,006 + -0.1 2,889,012 + -0.4 2,889,012 + -0.2 2,889,023 + -0.4 2,889,027 + -0.9 2,889,036 + -1.3 2,889,036 + +0.3 2,889,136 + CA265_RS11655 0.11 +0.9 2,889,136 + CA265_RS11655 0.11 -0.5 2,889,137 - CA265_RS11655 0.12 +0.4 2,889,140 - CA265_RS11655 0.12 -0.0 2,889,140 - CA265_RS11655 0.12 -0.7 2,889,199 + CA265_RS11655 0.20 +0.6 2,889,200 - CA265_RS11655 0.20 -0.6 2,889,220 + CA265_RS11655 0.23 -0.7 2,889,220 + CA265_RS11655 0.23 +0.7 2,889,273 + CA265_RS11655 0.30 -0.1 2,889,273 + CA265_RS11655 0.30 +0.3 2,889,274 - CA265_RS11655 0.30 +0.1 2,889,295 + CA265_RS11655 0.33 +1.1 2,889,302 + CA265_RS11655 0.33 -1.2 2,889,379 + CA265_RS11655 0.44 -1.3 2,889,500 - CA265_RS11655 0.60 -0.3 2,889,653 + CA265_RS11655 0.80 -0.8 2,889,656 - CA265_RS11655 0.80 -0.0 2,889,656 - CA265_RS11655 0.80 -1.1 2,889,662 - CA265_RS11655 0.81 -0.6 2,889,668 - CA265_RS11655 0.82 -1.3 2,889,679 - CA265_RS11655 0.84 -1.9 2,889,706 - CA265_RS11655 0.87 -2.5 2,889,731 - -3.7 2,889,848 + +1.1 2,889,876 + +0.5 2,889,881 + -0.1 2,889,930 - +0.9 2,890,174 - CA265_RS11660 0.16 -1.4 2,890,188 + CA265_RS11660 0.17 -0.0 2,890,188 + CA265_RS11660 0.17 +0.0 2,890,189 - CA265_RS11660 0.17 +0.7 2,890,189 - CA265_RS11660 0.17 -1.0 2,890,189 - CA265_RS11660 0.17 +0.5 2,890,189 - CA265_RS11660 0.17 -1.3 2,890,196 + CA265_RS11660 0.17 -2.3 2,890,255 - CA265_RS11660 0.19 +0.6 2,890,255 - CA265_RS11660 0.19 -0.1 2,890,267 + CA265_RS11660 0.20 -1.3 2,890,274 + CA265_RS11660 0.20 +0.1 2,890,331 + CA265_RS11660 0.23 -0.3 2,890,331 + CA265_RS11660 0.23 +0.0 2,890,331 + CA265_RS11660 0.23 +0.0 2,890,333 + CA265_RS11660 0.23 +0.1 2,890,334 - CA265_RS11660 0.23 -1.6 2,890,334 - CA265_RS11660 0.23 +0.3 2,890,334 - CA265_RS11660 0.23 +1.3 2,890,334 - CA265_RS11660 0.23 +0.7 2,890,371 + CA265_RS11660 0.24 -0.7 2,890,389 - CA265_RS11660 0.25 -0.3 2,890,456 - CA265_RS11660 0.28 -1.0 2,890,463 + CA265_RS11660 0.28 -0.1 2,890,470 - CA265_RS11660 0.29 -0.5 2,890,485 - CA265_RS11660 0.29 -0.2 2,890,489 - CA265_RS11660 0.30 +1.1 2,890,489 - CA265_RS11660 0.30 +0.4 2,890,489 - CA265_RS11660 0.30 -1.2 2,890,560 + CA265_RS11660 0.33 -0.2 2,890,583 + CA265_RS11660 0.34 +0.4 2,890,589 + CA265_RS11660 0.34 +0.2 2,890,589 + CA265_RS11660 0.34 +0.1 2,890,653 - CA265_RS11660 0.37 -0.4 2,890,668 + CA265_RS11660 0.37 -0.4 2,890,875 + CA265_RS11660 0.46 -0.7 2,890,876 - CA265_RS11660 0.46 +0.6 2,890,964 + CA265_RS11660 0.50 -0.2 2,890,973 + CA265_RS11660 0.50 -0.8 2,890,975 + CA265_RS11660 0.51 -0.7 2,890,975 + CA265_RS11660 0.51 -0.5 2,890,976 - CA265_RS11660 0.51 -0.3 2,890,987 + CA265_RS11660 0.51 +1.3 2,891,024 - CA265_RS11660 0.53 -1.3 2,891,025 + CA265_RS11660 0.53 -0.1 2,891,026 - CA265_RS11660 0.53 +0.1 2,891,026 - CA265_RS11660 0.53 +2.0 2,891,091 + CA265_RS11660 0.56 +0.0 2,891,106 + CA265_RS11660 0.56 +0.1 2,891,110 - CA265_RS11660 0.56 -0.9 2,891,163 + CA265_RS11660 0.59 +0.1 2,891,177 - CA265_RS11660 0.59 +0.4 2,891,187 - CA265_RS11660 0.60 -1.0 2,891,264 - CA265_RS11660 0.63 -0.4 2,891,344 + CA265_RS11660 0.66 -0.3 2,891,344 + CA265_RS11660 0.66 +0.5 2,891,345 - CA265_RS11660 0.67 -0.7 2,891,348 - CA265_RS11660 0.67 +1.1 2,891,352 + CA265_RS11660 0.67 -0.0 2,891,422 + CA265_RS11660 0.70 +0.3 2,891,444 - CA265_RS11660 0.71 -0.7 2,891,454 + CA265_RS11660 0.71 +0.3 2,891,466 - CA265_RS11660 0.72 +1.1 2,891,466 - CA265_RS11660 0.72 +1.1 2,891,509 + CA265_RS11660 0.74 +0.7 2,891,510 - CA265_RS11660 0.74 +0.5 2,891,510 - CA265_RS11660 0.74 -0.6 2,891,510 - CA265_RS11660 0.74 +0.3 2,891,510 - CA265_RS11660 0.74 -0.0 2,891,558 + CA265_RS11660 0.76 +1.0 2,891,565 - CA265_RS11660 0.76 +0.8 2,891,567 - CA265_RS11660 0.76 +1.2 2,891,567 - CA265_RS11660 0.76 +0.5 2,891,567 - CA265_RS11660 0.76 +1.5 2,891,634 + CA265_RS11660 0.79 +0.8 2,891,647 + CA265_RS11660 0.80 -0.0 2,891,647 + CA265_RS11660 0.80 +1.4 2,891,658 - CA265_RS11660 0.80 +1.7 2,891,666 - CA265_RS11660 0.80 +0.3 2,891,667 + CA265_RS11660 0.80 +0.7 2,891,668 - CA265_RS11660 0.80 +0.3 2,891,686 + CA265_RS11660 0.81 +0.9 2,891,687 - CA265_RS11660 0.81 -1.8 2,891,687 - CA265_RS11660 0.81 -1.1 2,891,703 - CA265_RS11660 0.82 -0.4 2,891,786 - CA265_RS11660 0.86 +1.3 2,891,790 + CA265_RS11660 0.86 +1.2 2,891,790 + CA265_RS11660 0.86 -0.1 2,891,791 - CA265_RS11660 0.86 +1.4 2,891,803 + CA265_RS11660 0.86 +0.5 2,891,862 - CA265_RS11660 0.89 +1.0 2,891,869 + CA265_RS11660 0.89 -0.8 2,891,883 - CA265_RS11660 0.90 -1.1 2,892,142 - -0.8 2,892,207 - +0.9 2,892,214 - -0.3 2,892,269 - -0.5 2,892,342 - -0.3
Or see this region's nucleotide sequence