Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11640 and CA265_RS11645 are separated by 103 nucleotides CA265_RS11645 and CA265_RS11650 are separated by 9 nucleotides CA265_RS11650 and CA265_RS11655 are separated by 125 nucleotides
CA265_RS11640: CA265_RS11640 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 2,885,837 to 2,887,105
_RS11640
CA265_RS11645: CA265_RS11645 - chorismate synthase, at 2,887,209 to 2,888,306
_RS11645
CA265_RS11650: CA265_RS11650 - hypothetical protein, at 2,888,316 to 2,888,924
_RS11650
CA265_RS11655: CA265_RS11655 - hypothetical protein, at 2,889,050 to 2,889,802
_RS11655
Position (kb)
2887
2888
2889 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2887.117 kb on + strand at 2887.128 kb on - strand at 2887.132 kb on - strand at 2887.175 kb on + strand at 2888.336 kb on - strand at 2888.341 kb on + strand at 2888.357 kb on + strand at 2888.358 kb on - strand at 2888.409 kb on - strand, within CA265_RS11650 at 2888.574 kb on + strand, within CA265_RS11650 at 2888.575 kb on - strand, within CA265_RS11650 at 2888.575 kb on - strand, within CA265_RS11650 at 2888.577 kb on + strand, within CA265_RS11650 at 2888.580 kb on - strand, within CA265_RS11650 at 2888.580 kb on - strand, within CA265_RS11650 at 2888.583 kb on + strand, within CA265_RS11650 at 2888.583 kb on + strand, within CA265_RS11650 at 2888.583 kb on + strand, within CA265_RS11650 at 2888.583 kb on + strand, within CA265_RS11650 at 2888.584 kb on - strand, within CA265_RS11650 at 2888.594 kb on - strand, within CA265_RS11650 at 2888.736 kb on + strand, within CA265_RS11650 at 2888.736 kb on + strand, within CA265_RS11650 at 2888.765 kb on - strand, within CA265_RS11650 at 2888.799 kb on + strand, within CA265_RS11650 at 2888.799 kb on + strand, within CA265_RS11650 at 2888.800 kb on - strand, within CA265_RS11650 at 2888.895 kb on + strand at 2888.895 kb on + strand at 2888.897 kb on + strand at 2888.897 kb on + strand at 2888.898 kb on - strand at 2888.898 kb on - strand at 2888.898 kb on - strand at 2888.955 kb on + strand at 2888.969 kb on + strand at 2888.972 kb on - strand at 2888.972 kb on - strand at 2888.972 kb on - strand at 2888.993 kb on + strand at 2889.006 kb on + strand at 2889.012 kb on + strand at 2889.012 kb on + strand at 2889.023 kb on + strand at 2889.027 kb on + strand at 2889.036 kb on + strand at 2889.036 kb on + strand at 2889.136 kb on + strand, within CA265_RS11655 at 2889.136 kb on + strand, within CA265_RS11655 at 2889.137 kb on - strand, within CA265_RS11655 at 2889.140 kb on - strand, within CA265_RS11655 at 2889.140 kb on - strand, within CA265_RS11655 at 2889.199 kb on + strand, within CA265_RS11655 at 2889.200 kb on - strand, within CA265_RS11655 at 2889.220 kb on + strand, within CA265_RS11655 at 2889.220 kb on + strand, within CA265_RS11655 at 2889.273 kb on + strand, within CA265_RS11655 at 2889.273 kb on + strand, within CA265_RS11655 at 2889.274 kb on - strand, within CA265_RS11655 at 2889.295 kb on + strand, within CA265_RS11655 at 2889.302 kb on + strand, within CA265_RS11655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,887,117 + +0.3 2,887,128 - -0.2 2,887,132 - -0.4 2,887,175 + -2.4 2,888,336 - -1.7 2,888,341 + +0.5 2,888,357 + -0.4 2,888,358 - +0.1 2,888,409 - CA265_RS11650 0.15 -1.0 2,888,574 + CA265_RS11650 0.42 -0.0 2,888,575 - CA265_RS11650 0.43 +0.4 2,888,575 - CA265_RS11650 0.43 +0.2 2,888,577 + CA265_RS11650 0.43 -0.3 2,888,580 - CA265_RS11650 0.43 -0.7 2,888,580 - CA265_RS11650 0.43 -0.8 2,888,583 + CA265_RS11650 0.44 +1.3 2,888,583 + CA265_RS11650 0.44 +0.6 2,888,583 + CA265_RS11650 0.44 -0.4 2,888,583 + CA265_RS11650 0.44 -2.6 2,888,584 - CA265_RS11650 0.44 -0.2 2,888,594 - CA265_RS11650 0.46 -0.5 2,888,736 + CA265_RS11650 0.69 +0.2 2,888,736 + CA265_RS11650 0.69 -0.1 2,888,765 - CA265_RS11650 0.74 -1.6 2,888,799 + CA265_RS11650 0.79 +2.3 2,888,799 + CA265_RS11650 0.79 +0.1 2,888,800 - CA265_RS11650 0.79 -1.3 2,888,895 + +0.8 2,888,895 + +0.7 2,888,897 + -0.8 2,888,897 + +0.4 2,888,898 - +0.5 2,888,898 - -0.3 2,888,898 - -0.7 2,888,955 + -0.1 2,888,969 + -1.1 2,888,972 - +1.6 2,888,972 - -0.7 2,888,972 - +1.5 2,888,993 + +0.7 2,889,006 + -0.1 2,889,012 + -0.4 2,889,012 + -0.2 2,889,023 + -0.4 2,889,027 + -0.9 2,889,036 + -1.3 2,889,036 + +0.3 2,889,136 + CA265_RS11655 0.11 +0.9 2,889,136 + CA265_RS11655 0.11 -0.5 2,889,137 - CA265_RS11655 0.12 +0.4 2,889,140 - CA265_RS11655 0.12 -0.0 2,889,140 - CA265_RS11655 0.12 -0.7 2,889,199 + CA265_RS11655 0.20 +0.6 2,889,200 - CA265_RS11655 0.20 -0.6 2,889,220 + CA265_RS11655 0.23 -0.7 2,889,220 + CA265_RS11655 0.23 +0.7 2,889,273 + CA265_RS11655 0.30 -0.1 2,889,273 + CA265_RS11655 0.30 +0.3 2,889,274 - CA265_RS11655 0.30 +0.1 2,889,295 + CA265_RS11655 0.33 +1.1 2,889,302 + CA265_RS11655 0.33 -1.2
Or see this region's nucleotide sequence