Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10220 and CA265_RS10225 are separated by 53 nucleotides CA265_RS10225 and CA265_RS10230 are separated by 6 nucleotides
CA265_RS10220: CA265_RS10220 - hypothetical protein, at 2,512,430 to 2,513,818
_RS10220
CA265_RS10225: CA265_RS10225 - hypothetical protein, at 2,513,872 to 2,515,242
_RS10225
CA265_RS10230: CA265_RS10230 - hypothetical protein, at 2,515,249 to 2,516,682
_RS10230
Position (kb)
2513
2514
2515
2516 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2512.873 kb on + strand, within CA265_RS10220 at 2512.873 kb on + strand, within CA265_RS10220 at 2512.874 kb on - strand, within CA265_RS10220 at 2512.926 kb on - strand, within CA265_RS10220 at 2512.981 kb on + strand, within CA265_RS10220 at 2513.209 kb on + strand, within CA265_RS10220 at 2513.269 kb on + strand, within CA265_RS10220 at 2513.270 kb on - strand, within CA265_RS10220 at 2513.296 kb on + strand, within CA265_RS10220 at 2513.316 kb on - strand, within CA265_RS10220 at 2513.371 kb on - strand, within CA265_RS10220 at 2513.451 kb on - strand, within CA265_RS10220 at 2513.458 kb on + strand, within CA265_RS10220 at 2513.458 kb on + strand, within CA265_RS10220 at 2513.459 kb on - strand, within CA265_RS10220 at 2513.459 kb on - strand, within CA265_RS10220 at 2513.461 kb on - strand, within CA265_RS10220 at 2513.481 kb on + strand, within CA265_RS10220 at 2513.481 kb on + strand, within CA265_RS10220 at 2513.482 kb on - strand, within CA265_RS10220 at 2513.482 kb on - strand, within CA265_RS10220 at 2513.482 kb on - strand, within CA265_RS10220 at 2513.522 kb on - strand, within CA265_RS10220 at 2513.524 kb on - strand, within CA265_RS10220 at 2513.585 kb on - strand, within CA265_RS10220 at 2513.631 kb on + strand, within CA265_RS10220 at 2513.632 kb on - strand, within CA265_RS10220 at 2513.639 kb on - strand, within CA265_RS10220 at 2513.695 kb on - strand at 2513.695 kb on - strand at 2513.745 kb on - strand at 2513.793 kb on - strand at 2513.798 kb on - strand at 2513.820 kb on - strand at 2513.874 kb on - strand at 2514.018 kb on + strand, within CA265_RS10225 at 2514.045 kb on + strand, within CA265_RS10225 at 2514.049 kb on + strand, within CA265_RS10225 at 2514.093 kb on - strand, within CA265_RS10225 at 2514.108 kb on - strand, within CA265_RS10225 at 2514.166 kb on - strand, within CA265_RS10225 at 2514.177 kb on + strand, within CA265_RS10225 at 2514.252 kb on + strand, within CA265_RS10225 at 2514.252 kb on + strand, within CA265_RS10225 at 2514.297 kb on + strand, within CA265_RS10225 at 2514.343 kb on - strand, within CA265_RS10225 at 2514.410 kb on + strand, within CA265_RS10225 at 2514.415 kb on + strand, within CA265_RS10225 at 2514.455 kb on + strand, within CA265_RS10225 at 2514.456 kb on - strand, within CA265_RS10225 at 2514.456 kb on - strand, within CA265_RS10225 at 2514.494 kb on + strand, within CA265_RS10225 at 2514.564 kb on + strand, within CA265_RS10225 at 2514.565 kb on - strand, within CA265_RS10225 at 2514.616 kb on - strand, within CA265_RS10225 at 2514.676 kb on + strand, within CA265_RS10225 at 2514.685 kb on + strand, within CA265_RS10225 at 2514.753 kb on + strand, within CA265_RS10225 at 2514.753 kb on + strand, within CA265_RS10225 at 2514.833 kb on + strand, within CA265_RS10225 at 2514.847 kb on - strand, within CA265_RS10225 at 2514.857 kb on - strand, within CA265_RS10225 at 2514.862 kb on + strand, within CA265_RS10225 at 2514.863 kb on - strand, within CA265_RS10225 at 2514.869 kb on - strand, within CA265_RS10225 at 2514.878 kb on + strand, within CA265_RS10225 at 2514.879 kb on - strand, within CA265_RS10225 at 2514.894 kb on + strand, within CA265_RS10225 at 2514.900 kb on + strand, within CA265_RS10225 at 2514.900 kb on + strand, within CA265_RS10225 at 2514.901 kb on - strand, within CA265_RS10225 at 2514.901 kb on - strand, within CA265_RS10225 at 2514.915 kb on + strand, within CA265_RS10225 at 2514.916 kb on - strand, within CA265_RS10225 at 2514.963 kb on + strand, within CA265_RS10225 at 2514.969 kb on - strand, within CA265_RS10225 at 2514.993 kb on - strand, within CA265_RS10225 at 2514.993 kb on - strand, within CA265_RS10225 at 2515.041 kb on + strand, within CA265_RS10225 at 2515.074 kb on - strand, within CA265_RS10225 at 2515.098 kb on + strand, within CA265_RS10225 at 2515.098 kb on + strand, within CA265_RS10225 at 2515.123 kb on - strand at 2515.140 kb on + strand at 2515.146 kb on + strand at 2515.179 kb on + strand at 2515.179 kb on + strand at 2515.217 kb on - strand at 2515.264 kb on + strand at 2515.265 kb on - strand at 2515.327 kb on + strand at 2515.348 kb on - strand at 2515.359 kb on - strand at 2515.399 kb on + strand, within CA265_RS10230 at 2515.399 kb on + strand, within CA265_RS10230 at 2515.399 kb on + strand, within CA265_RS10230 at 2515.400 kb on - strand, within CA265_RS10230 at 2515.443 kb on + strand, within CA265_RS10230 at 2515.444 kb on - strand, within CA265_RS10230 at 2515.465 kb on + strand, within CA265_RS10230 at 2515.465 kb on + strand, within CA265_RS10230 at 2515.503 kb on + strand, within CA265_RS10230 at 2515.516 kb on - strand, within CA265_RS10230 at 2515.556 kb on + strand, within CA265_RS10230 at 2515.610 kb on + strand, within CA265_RS10230 at 2515.632 kb on - strand, within CA265_RS10230 at 2515.704 kb on + strand, within CA265_RS10230 at 2515.707 kb on - strand, within CA265_RS10230 at 2515.719 kb on + strand, within CA265_RS10230 at 2515.742 kb on + strand, within CA265_RS10230 at 2515.753 kb on - strand, within CA265_RS10230 at 2515.760 kb on - strand, within CA265_RS10230 at 2515.795 kb on - strand, within CA265_RS10230 at 2515.817 kb on + strand, within CA265_RS10230 at 2515.861 kb on + strand, within CA265_RS10230 at 2515.861 kb on + strand, within CA265_RS10230 at 2515.862 kb on - strand, within CA265_RS10230 at 2515.862 kb on - strand, within CA265_RS10230 at 2515.957 kb on + strand, within CA265_RS10230 at 2515.958 kb on - strand, within CA265_RS10230 at 2515.958 kb on - strand, within CA265_RS10230 at 2515.984 kb on - strand, within CA265_RS10230 at 2515.987 kb on + strand, within CA265_RS10230 at 2515.987 kb on + strand, within CA265_RS10230 at 2516.113 kb on + strand, within CA265_RS10230 at 2516.114 kb on - strand, within CA265_RS10230 at 2516.124 kb on + strand, within CA265_RS10230 at 2516.151 kb on - strand, within CA265_RS10230 at 2516.151 kb on - strand, within CA265_RS10230 at 2516.169 kb on + strand, within CA265_RS10230 at 2516.179 kb on - strand, within CA265_RS10230 at 2516.235 kb on + strand, within CA265_RS10230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,512,873 + CA265_RS10220 0.32 -2.8 2,512,873 + CA265_RS10220 0.32 -1.4 2,512,874 - CA265_RS10220 0.32 -0.1 2,512,926 - CA265_RS10220 0.36 -0.2 2,512,981 + CA265_RS10220 0.40 +0.1 2,513,209 + CA265_RS10220 0.56 -0.0 2,513,269 + CA265_RS10220 0.60 +0.3 2,513,270 - CA265_RS10220 0.60 +0.3 2,513,296 + CA265_RS10220 0.62 +0.0 2,513,316 - CA265_RS10220 0.64 +0.9 2,513,371 - CA265_RS10220 0.68 +0.6 2,513,451 - CA265_RS10220 0.74 +0.9 2,513,458 + CA265_RS10220 0.74 -0.3 2,513,458 + CA265_RS10220 0.74 +0.2 2,513,459 - CA265_RS10220 0.74 +0.4 2,513,459 - CA265_RS10220 0.74 +0.4 2,513,461 - CA265_RS10220 0.74 -0.3 2,513,481 + CA265_RS10220 0.76 -1.7 2,513,481 + CA265_RS10220 0.76 +1.6 2,513,482 - CA265_RS10220 0.76 +0.1 2,513,482 - CA265_RS10220 0.76 -1.3 2,513,482 - CA265_RS10220 0.76 +0.6 2,513,522 - CA265_RS10220 0.79 +0.5 2,513,524 - CA265_RS10220 0.79 +1.3 2,513,585 - CA265_RS10220 0.83 -0.8 2,513,631 + CA265_RS10220 0.86 +0.5 2,513,632 - CA265_RS10220 0.87 +0.9 2,513,639 - CA265_RS10220 0.87 +0.2 2,513,695 - -0.7 2,513,695 - -1.3 2,513,745 - -0.2 2,513,793 - +1.0 2,513,798 - +0.2 2,513,820 - -0.3 2,513,874 - +0.9 2,514,018 + CA265_RS10225 0.11 -0.3 2,514,045 + CA265_RS10225 0.13 +0.8 2,514,049 + CA265_RS10225 0.13 -0.9 2,514,093 - CA265_RS10225 0.16 +1.0 2,514,108 - CA265_RS10225 0.17 +0.9 2,514,166 - CA265_RS10225 0.21 -0.4 2,514,177 + CA265_RS10225 0.22 +0.1 2,514,252 + CA265_RS10225 0.28 +0.4 2,514,252 + CA265_RS10225 0.28 +0.5 2,514,297 + CA265_RS10225 0.31 -0.2 2,514,343 - CA265_RS10225 0.34 +1.1 2,514,410 + CA265_RS10225 0.39 -1.9 2,514,415 + CA265_RS10225 0.40 +1.6 2,514,455 + CA265_RS10225 0.43 -1.2 2,514,456 - CA265_RS10225 0.43 +0.5 2,514,456 - CA265_RS10225 0.43 -0.1 2,514,494 + CA265_RS10225 0.45 -1.1 2,514,564 + CA265_RS10225 0.50 +0.4 2,514,565 - CA265_RS10225 0.51 +1.6 2,514,616 - CA265_RS10225 0.54 +1.1 2,514,676 + CA265_RS10225 0.59 -0.7 2,514,685 + CA265_RS10225 0.59 +0.2 2,514,753 + CA265_RS10225 0.64 -0.3 2,514,753 + CA265_RS10225 0.64 +0.8 2,514,833 + CA265_RS10225 0.70 +0.6 2,514,847 - CA265_RS10225 0.71 +0.2 2,514,857 - CA265_RS10225 0.72 +1.1 2,514,862 + CA265_RS10225 0.72 +0.9 2,514,863 - CA265_RS10225 0.72 +1.7 2,514,869 - CA265_RS10225 0.73 +0.8 2,514,878 + CA265_RS10225 0.73 +0.1 2,514,879 - CA265_RS10225 0.73 +0.8 2,514,894 + CA265_RS10225 0.75 -0.5 2,514,900 + CA265_RS10225 0.75 +0.3 2,514,900 + CA265_RS10225 0.75 -0.7 2,514,901 - CA265_RS10225 0.75 -0.0 2,514,901 - CA265_RS10225 0.75 -0.4 2,514,915 + CA265_RS10225 0.76 +0.2 2,514,916 - CA265_RS10225 0.76 +0.8 2,514,963 + CA265_RS10225 0.80 +0.8 2,514,969 - CA265_RS10225 0.80 +3.0 2,514,993 - CA265_RS10225 0.82 -2.6 2,514,993 - CA265_RS10225 0.82 -0.1 2,515,041 + CA265_RS10225 0.85 +0.4 2,515,074 - CA265_RS10225 0.88 -0.3 2,515,098 + CA265_RS10225 0.89 +0.4 2,515,098 + CA265_RS10225 0.89 -0.3 2,515,123 - +0.3 2,515,140 + -1.1 2,515,146 + -0.5 2,515,179 + -0.2 2,515,179 + -0.2 2,515,217 - +1.3 2,515,264 + -0.0 2,515,265 - +0.7 2,515,327 + -1.0 2,515,348 - -0.5 2,515,359 - +0.2 2,515,399 + CA265_RS10230 0.10 +1.0 2,515,399 + CA265_RS10230 0.10 +1.3 2,515,399 + CA265_RS10230 0.10 -1.0 2,515,400 - CA265_RS10230 0.11 +0.5 2,515,443 + CA265_RS10230 0.14 -0.3 2,515,444 - CA265_RS10230 0.14 +0.4 2,515,465 + CA265_RS10230 0.15 -0.4 2,515,465 + CA265_RS10230 0.15 -0.2 2,515,503 + CA265_RS10230 0.18 -0.1 2,515,516 - CA265_RS10230 0.19 +0.2 2,515,556 + CA265_RS10230 0.21 +0.7 2,515,610 + CA265_RS10230 0.25 -1.6 2,515,632 - CA265_RS10230 0.27 +0.5 2,515,704 + CA265_RS10230 0.32 -0.0 2,515,707 - CA265_RS10230 0.32 -0.9 2,515,719 + CA265_RS10230 0.33 +0.4 2,515,742 + CA265_RS10230 0.34 -1.0 2,515,753 - CA265_RS10230 0.35 +0.4 2,515,760 - CA265_RS10230 0.36 +0.2 2,515,795 - CA265_RS10230 0.38 +0.2 2,515,817 + CA265_RS10230 0.40 +0.7 2,515,861 + CA265_RS10230 0.43 -1.2 2,515,861 + CA265_RS10230 0.43 +0.7 2,515,862 - CA265_RS10230 0.43 +0.9 2,515,862 - CA265_RS10230 0.43 +1.1 2,515,957 + CA265_RS10230 0.49 +0.4 2,515,958 - CA265_RS10230 0.49 +0.1 2,515,958 - CA265_RS10230 0.49 +0.6 2,515,984 - CA265_RS10230 0.51 +0.5 2,515,987 + CA265_RS10230 0.51 +0.2 2,515,987 + CA265_RS10230 0.51 +0.1 2,516,113 + CA265_RS10230 0.60 -0.0 2,516,114 - CA265_RS10230 0.60 -0.5 2,516,124 + CA265_RS10230 0.61 +1.7 2,516,151 - CA265_RS10230 0.63 +0.5 2,516,151 - CA265_RS10230 0.63 +0.2 2,516,169 + CA265_RS10230 0.64 +0.5 2,516,179 - CA265_RS10230 0.65 +1.3 2,516,235 + CA265_RS10230 0.69 -0.6
Or see this region's nucleotide sequence