Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09300 and CA265_RS09305 overlap by 1 nucleotides CA265_RS09305 and CA265_RS09310 are separated by 41 nucleotides CA265_RS09310 and CA265_RS09315 are separated by 38 nucleotides CA265_RS09315 and CA265_RS09320 are separated by 48 nucleotides
CA265_RS09300: CA265_RS09300 - hypothetical protein, at 2,313,914 to 2,314,774
_RS09300
CA265_RS09305: CA265_RS09305 - histone deacetylase, at 2,314,774 to 2,315,676
_RS09305
CA265_RS09310: CA265_RS09310 - pseudouridylate synthase, at 2,315,718 to 2,316,401
_RS09310
CA265_RS09315: CA265_RS09315 - hypothetical protein, at 2,316,440 to 2,317,174
_RS09315
CA265_RS09320: CA265_RS09320 - hypothetical protein, at 2,317,223 to 2,317,417
_RS09320
Position (kb)
2315
2316
2317 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2314.801 kb on + strand at 2314.930 kb on - strand, within CA265_RS09305 at 2314.958 kb on - strand, within CA265_RS09305 at 2315.037 kb on - strand, within CA265_RS09305 at 2315.091 kb on - strand, within CA265_RS09305 at 2315.095 kb on - strand, within CA265_RS09305 at 2315.110 kb on - strand, within CA265_RS09305 at 2315.110 kb on - strand, within CA265_RS09305 at 2315.166 kb on - strand, within CA265_RS09305 at 2315.213 kb on + strand, within CA265_RS09305 at 2315.240 kb on + strand at 2315.246 kb on + strand, within CA265_RS09305 at 2315.246 kb on + strand, within CA265_RS09305 at 2315.366 kb on + strand, within CA265_RS09305 at 2315.392 kb on + strand, within CA265_RS09305 at 2315.393 kb on - strand, within CA265_RS09305 at 2315.393 kb on - strand, within CA265_RS09305 at 2315.405 kb on + strand, within CA265_RS09305 at 2315.417 kb on + strand, within CA265_RS09305 at 2315.439 kb on + strand, within CA265_RS09305 at 2315.458 kb on + strand, within CA265_RS09305 at 2315.459 kb on - strand, within CA265_RS09305 at 2315.462 kb on + strand, within CA265_RS09305 at 2315.471 kb on + strand, within CA265_RS09305 at 2315.472 kb on - strand, within CA265_RS09305 at 2315.491 kb on - strand, within CA265_RS09305 at 2315.544 kb on - strand, within CA265_RS09305 at 2315.544 kb on - strand, within CA265_RS09305 at 2315.546 kb on + strand, within CA265_RS09305 at 2315.589 kb on + strand at 2315.622 kb on + strand at 2315.628 kb on - strand at 2315.694 kb on + strand at 2315.718 kb on - strand at 2315.734 kb on - strand at 2315.760 kb on - strand at 2315.833 kb on + strand, within CA265_RS09310 at 2315.916 kb on - strand, within CA265_RS09310 at 2315.934 kb on - strand, within CA265_RS09310 at 2315.949 kb on - strand, within CA265_RS09310 at 2316.071 kb on + strand, within CA265_RS09310 at 2316.072 kb on - strand, within CA265_RS09310 at 2316.139 kb on + strand, within CA265_RS09310 at 2316.146 kb on + strand, within CA265_RS09310 at 2316.146 kb on + strand, within CA265_RS09310 at 2316.176 kb on - strand, within CA265_RS09310 at 2316.199 kb on + strand, within CA265_RS09310 at 2316.199 kb on + strand, within CA265_RS09310 at 2316.353 kb on - strand at 2316.432 kb on + strand at 2316.433 kb on - strand at 2316.439 kb on + strand at 2316.439 kb on + strand at 2316.440 kb on - strand at 2316.440 kb on - strand at 2316.453 kb on + strand at 2316.506 kb on + strand at 2316.506 kb on + strand at 2316.507 kb on - strand at 2316.507 kb on - strand at 2316.509 kb on - strand at 2316.526 kb on + strand, within CA265_RS09315 at 2316.527 kb on - strand, within CA265_RS09315 at 2316.537 kb on + strand, within CA265_RS09315 at 2316.542 kb on - strand, within CA265_RS09315 at 2316.634 kb on - strand, within CA265_RS09315 at 2316.788 kb on - strand, within CA265_RS09315 at 2316.815 kb on - strand, within CA265_RS09315 at 2316.864 kb on + strand, within CA265_RS09315 at 2316.865 kb on - strand, within CA265_RS09315 at 2316.929 kb on - strand, within CA265_RS09315 at 2316.929 kb on - strand, within CA265_RS09315 at 2316.933 kb on - strand, within CA265_RS09315 at 2316.958 kb on + strand, within CA265_RS09315 at 2316.962 kb on - strand, within CA265_RS09315 at 2317.043 kb on - strand, within CA265_RS09315 at 2317.075 kb on + strand, within CA265_RS09315 at 2317.090 kb on + strand, within CA265_RS09315 at 2317.090 kb on + strand, within CA265_RS09315 at 2317.168 kb on + strand at 2317.169 kb on - strand at 2317.172 kb on + strand at 2317.173 kb on - strand at 2317.193 kb on + strand at 2317.194 kb on - strand at 2317.210 kb on - strand at 2317.213 kb on + strand at 2317.214 kb on - strand at 2317.325 kb on - strand, within CA265_RS09320 at 2317.325 kb on - strand, within CA265_RS09320 at 2317.396 kb on + strand, within CA265_RS09320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,314,801 + +0.6 2,314,930 - CA265_RS09305 0.17 -0.6 2,314,958 - CA265_RS09305 0.20 -0.3 2,315,037 - CA265_RS09305 0.29 +1.1 2,315,091 - CA265_RS09305 0.35 +0.2 2,315,095 - CA265_RS09305 0.36 -0.1 2,315,110 - CA265_RS09305 0.37 -3.0 2,315,110 - CA265_RS09305 0.37 -0.4 2,315,166 - CA265_RS09305 0.43 -1.1 2,315,213 + CA265_RS09305 0.49 -1.1 2,315,240 + -0.0 2,315,246 + CA265_RS09305 0.52 -0.2 2,315,246 + CA265_RS09305 0.52 +0.1 2,315,366 + CA265_RS09305 0.66 -0.8 2,315,392 + CA265_RS09305 0.68 -0.4 2,315,393 - CA265_RS09305 0.69 -0.0 2,315,393 - CA265_RS09305 0.69 -0.7 2,315,405 + CA265_RS09305 0.70 -0.3 2,315,417 + CA265_RS09305 0.71 +1.3 2,315,439 + CA265_RS09305 0.74 -1.5 2,315,458 + CA265_RS09305 0.76 -2.6 2,315,459 - CA265_RS09305 0.76 -0.3 2,315,462 + CA265_RS09305 0.76 -0.3 2,315,471 + CA265_RS09305 0.77 -0.0 2,315,472 - CA265_RS09305 0.77 -0.9 2,315,491 - CA265_RS09305 0.79 -0.4 2,315,544 - CA265_RS09305 0.85 +0.7 2,315,544 - CA265_RS09305 0.85 -0.1 2,315,546 + CA265_RS09305 0.85 -1.8 2,315,589 + +0.4 2,315,622 + -1.7 2,315,628 - -1.2 2,315,694 + -0.5 2,315,718 - -0.1 2,315,734 - -0.0 2,315,760 - -0.7 2,315,833 + CA265_RS09310 0.17 -0.3 2,315,916 - CA265_RS09310 0.29 +0.1 2,315,934 - CA265_RS09310 0.32 +0.0 2,315,949 - CA265_RS09310 0.34 +0.8 2,316,071 + CA265_RS09310 0.52 -2.1 2,316,072 - CA265_RS09310 0.52 +0.9 2,316,139 + CA265_RS09310 0.62 -1.5 2,316,146 + CA265_RS09310 0.63 -0.6 2,316,146 + CA265_RS09310 0.63 -0.3 2,316,176 - CA265_RS09310 0.67 +0.5 2,316,199 + CA265_RS09310 0.70 +0.3 2,316,199 + CA265_RS09310 0.70 -2.0 2,316,353 - +0.7 2,316,432 + +0.3 2,316,433 - +2.4 2,316,439 + -0.9 2,316,439 + -0.5 2,316,440 - +0.7 2,316,440 - -0.6 2,316,453 + -0.5 2,316,506 + -0.2 2,316,506 + +1.3 2,316,507 - -0.5 2,316,507 - -0.4 2,316,509 - -0.4 2,316,526 + CA265_RS09315 0.12 +0.7 2,316,527 - CA265_RS09315 0.12 -0.9 2,316,537 + CA265_RS09315 0.13 -0.6 2,316,542 - CA265_RS09315 0.14 +1.0 2,316,634 - CA265_RS09315 0.26 -0.2 2,316,788 - CA265_RS09315 0.47 -0.4 2,316,815 - CA265_RS09315 0.51 -0.4 2,316,864 + CA265_RS09315 0.58 +0.1 2,316,865 - CA265_RS09315 0.58 +0.3 2,316,929 - CA265_RS09315 0.67 +0.4 2,316,929 - CA265_RS09315 0.67 +0.5 2,316,933 - CA265_RS09315 0.67 +0.1 2,316,958 + CA265_RS09315 0.70 +0.3 2,316,962 - CA265_RS09315 0.71 +1.6 2,317,043 - CA265_RS09315 0.82 +0.4 2,317,075 + CA265_RS09315 0.86 +1.6 2,317,090 + CA265_RS09315 0.88 -0.7 2,317,090 + CA265_RS09315 0.88 +0.6 2,317,168 + +0.2 2,317,169 - -1.3 2,317,172 + -0.5 2,317,173 - +0.2 2,317,193 + -0.2 2,317,194 - -0.1 2,317,210 - -0.5 2,317,213 + -0.6 2,317,214 - +1.7 2,317,325 - CA265_RS09320 0.52 -0.3 2,317,325 - CA265_RS09320 0.52 -0.2 2,317,396 + CA265_RS09320 0.89 -0.0
Or see this region's nucleotide sequence