Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09045 and CA265_RS09050 are separated by 169 nucleotides CA265_RS09050 and CA265_RS09055 are separated by 12 nucleotides CA265_RS09055 and CA265_RS09060 are separated by 74 nucleotides
CA265_RS09045: CA265_RS09045 - ABC transporter permease, at 2,253,930 to 2,254,883
_RS09045
CA265_RS09050: CA265_RS09050 - N-acetylmuramoyl-L-alanine amidase, at 2,255,053 to 2,255,829
_RS09050
CA265_RS09055: CA265_RS09055 - N-acetylmuramoyl-L-alanine amidase, at 2,255,842 to 2,256,705
_RS09055
CA265_RS09060: CA265_RS09060 - hypothetical protein, at 2,256,780 to 2,259,440
_RS09060
Position (kb)
2255
2256
2257 Strain fitness (log2 ratio)
-2
-1
0
1 at 2254.857 kb on + strand at 2254.893 kb on - strand at 2254.903 kb on + strand at 2254.904 kb on - strand at 2254.904 kb on - strand at 2254.904 kb on - strand at 2254.917 kb on + strand at 2254.974 kb on - strand at 2255.032 kb on - strand at 2255.100 kb on + strand at 2255.139 kb on + strand, within CA265_RS09050 at 2255.267 kb on + strand, within CA265_RS09050 at 2255.267 kb on + strand, within CA265_RS09050 at 2255.282 kb on - strand, within CA265_RS09050 at 2255.347 kb on - strand, within CA265_RS09050 at 2255.376 kb on - strand, within CA265_RS09050 at 2255.484 kb on + strand, within CA265_RS09050 at 2255.484 kb on + strand, within CA265_RS09050 at 2255.512 kb on - strand, within CA265_RS09050 at 2255.554 kb on + strand, within CA265_RS09050 at 2255.638 kb on + strand, within CA265_RS09050 at 2255.755 kb on - strand at 2255.804 kb on - strand at 2255.820 kb on + strand at 2255.863 kb on - strand at 2255.886 kb on + strand at 2255.952 kb on + strand, within CA265_RS09055 at 2256.025 kb on + strand, within CA265_RS09055 at 2256.056 kb on + strand, within CA265_RS09055 at 2256.057 kb on - strand, within CA265_RS09055 at 2256.085 kb on + strand, within CA265_RS09055 at 2256.126 kb on + strand, within CA265_RS09055 at 2256.127 kb on - strand, within CA265_RS09055 at 2256.127 kb on - strand, within CA265_RS09055 at 2256.127 kb on - strand, within CA265_RS09055 at 2256.127 kb on - strand, within CA265_RS09055 at 2256.213 kb on + strand, within CA265_RS09055 at 2256.214 kb on - strand, within CA265_RS09055 at 2256.236 kb on - strand, within CA265_RS09055 at 2256.236 kb on - strand, within CA265_RS09055 at 2256.295 kb on - strand, within CA265_RS09055 at 2256.295 kb on - strand, within CA265_RS09055 at 2256.306 kb on - strand, within CA265_RS09055 at 2256.339 kb on - strand, within CA265_RS09055 at 2256.342 kb on - strand, within CA265_RS09055 at 2256.398 kb on - strand, within CA265_RS09055 at 2256.438 kb on - strand, within CA265_RS09055 at 2256.471 kb on - strand, within CA265_RS09055 at 2256.483 kb on + strand, within CA265_RS09055 at 2256.714 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,254,857 + -0.7 2,254,893 - +0.0 2,254,903 + -0.9 2,254,904 - +0.3 2,254,904 - -1.7 2,254,904 - -0.9 2,254,917 + -1.0 2,254,974 - -0.6 2,255,032 - +0.6 2,255,100 + +0.6 2,255,139 + CA265_RS09050 0.11 -0.5 2,255,267 + CA265_RS09050 0.28 +0.4 2,255,267 + CA265_RS09050 0.28 +1.3 2,255,282 - CA265_RS09050 0.29 -1.9 2,255,347 - CA265_RS09050 0.38 -1.0 2,255,376 - CA265_RS09050 0.42 -0.5 2,255,484 + CA265_RS09050 0.55 +0.4 2,255,484 + CA265_RS09050 0.55 +1.0 2,255,512 - CA265_RS09050 0.59 +0.2 2,255,554 + CA265_RS09050 0.64 -0.3 2,255,638 + CA265_RS09050 0.75 +1.3 2,255,755 - +0.9 2,255,804 - -1.2 2,255,820 + +0.9 2,255,863 - -1.9 2,255,886 + -0.4 2,255,952 + CA265_RS09055 0.13 -2.6 2,256,025 + CA265_RS09055 0.21 -0.7 2,256,056 + CA265_RS09055 0.25 -0.0 2,256,057 - CA265_RS09055 0.25 -2.3 2,256,085 + CA265_RS09055 0.28 -0.7 2,256,126 + CA265_RS09055 0.33 -2.2 2,256,127 - CA265_RS09055 0.33 -2.2 2,256,127 - CA265_RS09055 0.33 -0.7 2,256,127 - CA265_RS09055 0.33 -1.4 2,256,127 - CA265_RS09055 0.33 -0.6 2,256,213 + CA265_RS09055 0.43 -1.9 2,256,214 - CA265_RS09055 0.43 -0.4 2,256,236 - CA265_RS09055 0.46 -1.0 2,256,236 - CA265_RS09055 0.46 -0.5 2,256,295 - CA265_RS09055 0.52 -0.0 2,256,295 - CA265_RS09055 0.52 -1.4 2,256,306 - CA265_RS09055 0.54 -0.5 2,256,339 - CA265_RS09055 0.58 -2.3 2,256,342 - CA265_RS09055 0.58 -1.2 2,256,398 - CA265_RS09055 0.64 -0.7 2,256,438 - CA265_RS09055 0.69 -1.6 2,256,471 - CA265_RS09055 0.73 -0.6 2,256,483 + CA265_RS09055 0.74 -0.3 2,256,714 + -1.2
Or see this region's nucleotide sequence