Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08600 and CA265_RS08605 are separated by 99 nucleotides CA265_RS08605 and CA265_RS08610 are separated by 81 nucleotides
CA265_RS08600: CA265_RS08600 - hypothetical protein, at 2,156,544 to 2,158,784
_RS08600
CA265_RS08605: CA265_RS08605 - short-chain dehydrogenase, at 2,158,884 to 2,159,672
_RS08605
CA265_RS08610: CA265_RS08610 - alcohol dehydrogenase, at 2,159,754 to 2,160,773
_RS08610
Position (kb)
2158
2159
2160 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2157.912 kb on - strand, within CA265_RS08600 at 2157.937 kb on - strand, within CA265_RS08600 at 2157.956 kb on + strand, within CA265_RS08600 at 2158.011 kb on + strand, within CA265_RS08600 at 2158.011 kb on + strand, within CA265_RS08600 at 2158.066 kb on + strand, within CA265_RS08600 at 2158.069 kb on - strand, within CA265_RS08600 at 2158.112 kb on + strand, within CA265_RS08600 at 2158.153 kb on + strand, within CA265_RS08600 at 2158.480 kb on + strand, within CA265_RS08600 at 2158.481 kb on - strand, within CA265_RS08600 at 2158.485 kb on - strand, within CA265_RS08600 at 2158.487 kb on - strand, within CA265_RS08600 at 2158.626 kb on + strand at 2158.674 kb on + strand at 2158.696 kb on + strand at 2158.771 kb on + strand at 2158.814 kb on + strand at 2158.869 kb on + strand at 2158.869 kb on + strand at 2158.898 kb on - strand at 2158.910 kb on + strand at 2158.960 kb on + strand at 2158.961 kb on - strand at 2159.078 kb on - strand, within CA265_RS08605 at 2159.119 kb on + strand, within CA265_RS08605 at 2159.150 kb on - strand, within CA265_RS08605 at 2159.154 kb on - strand, within CA265_RS08605 at 2159.196 kb on + strand, within CA265_RS08605 at 2159.196 kb on + strand, within CA265_RS08605 at 2159.197 kb on - strand, within CA265_RS08605 at 2159.198 kb on + strand, within CA265_RS08605 at 2159.238 kb on + strand, within CA265_RS08605 at 2159.238 kb on + strand, within CA265_RS08605 at 2159.241 kb on + strand, within CA265_RS08605 at 2159.336 kb on + strand, within CA265_RS08605 at 2159.336 kb on + strand, within CA265_RS08605 at 2159.336 kb on + strand, within CA265_RS08605 at 2159.358 kb on - strand, within CA265_RS08605 at 2159.377 kb on + strand, within CA265_RS08605 at 2159.409 kb on + strand, within CA265_RS08605 at 2159.410 kb on - strand, within CA265_RS08605 at 2159.582 kb on - strand, within CA265_RS08605 at 2159.638 kb on - strand at 2159.647 kb on + strand at 2159.648 kb on - strand at 2159.648 kb on - strand at 2159.651 kb on + strand at 2159.708 kb on + strand at 2159.708 kb on + strand at 2159.731 kb on + strand at 2159.751 kb on + strand at 2159.771 kb on + strand at 2159.840 kb on - strand at 2160.026 kb on + strand, within CA265_RS08610 at 2160.050 kb on + strand, within CA265_RS08610 at 2160.082 kb on + strand, within CA265_RS08610 at 2160.083 kb on - strand, within CA265_RS08610 at 2160.137 kb on - strand, within CA265_RS08610 at 2160.238 kb on + strand, within CA265_RS08610 at 2160.239 kb on - strand, within CA265_RS08610 at 2160.273 kb on - strand, within CA265_RS08610 at 2160.446 kb on - strand, within CA265_RS08610 at 2160.446 kb on - strand, within CA265_RS08610 at 2160.479 kb on + strand, within CA265_RS08610 at 2160.488 kb on - strand, within CA265_RS08610 at 2160.494 kb on + strand, within CA265_RS08610 at 2160.544 kb on + strand, within CA265_RS08610 at 2160.628 kb on + strand, within CA265_RS08610 at 2160.629 kb on - strand, within CA265_RS08610 at 2160.670 kb on - strand, within CA265_RS08610 at 2160.670 kb on - strand, within CA265_RS08610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 2,157,912 - CA265_RS08600 0.61 -0.3 2,157,937 - CA265_RS08600 0.62 -0.3 2,157,956 + CA265_RS08600 0.63 -0.1 2,158,011 + CA265_RS08600 0.65 +0.8 2,158,011 + CA265_RS08600 0.65 -0.4 2,158,066 + CA265_RS08600 0.68 +0.7 2,158,069 - CA265_RS08600 0.68 +1.0 2,158,112 + CA265_RS08600 0.70 -0.2 2,158,153 + CA265_RS08600 0.72 +0.9 2,158,480 + CA265_RS08600 0.86 +1.3 2,158,481 - CA265_RS08600 0.86 -0.4 2,158,485 - CA265_RS08600 0.87 -0.3 2,158,487 - CA265_RS08600 0.87 +0.1 2,158,626 + -2.1 2,158,674 + -1.5 2,158,696 + -0.1 2,158,771 + +0.7 2,158,814 + +0.4 2,158,869 + +0.0 2,158,869 + -1.0 2,158,898 - +0.4 2,158,910 + -0.6 2,158,960 + +1.1 2,158,961 - +1.0 2,159,078 - CA265_RS08605 0.25 -0.4 2,159,119 + CA265_RS08605 0.30 +0.6 2,159,150 - CA265_RS08605 0.34 -0.8 2,159,154 - CA265_RS08605 0.34 -0.0 2,159,196 + CA265_RS08605 0.40 +1.9 2,159,196 + CA265_RS08605 0.40 -1.2 2,159,197 - CA265_RS08605 0.40 -0.5 2,159,198 + CA265_RS08605 0.40 -0.4 2,159,238 + CA265_RS08605 0.45 +2.0 2,159,238 + CA265_RS08605 0.45 +1.1 2,159,241 + CA265_RS08605 0.45 +0.2 2,159,336 + CA265_RS08605 0.57 +0.7 2,159,336 + CA265_RS08605 0.57 +0.7 2,159,336 + CA265_RS08605 0.57 +1.0 2,159,358 - CA265_RS08605 0.60 -1.6 2,159,377 + CA265_RS08605 0.62 -0.5 2,159,409 + CA265_RS08605 0.67 +0.6 2,159,410 - CA265_RS08605 0.67 -0.8 2,159,582 - CA265_RS08605 0.88 -0.7 2,159,638 - -1.3 2,159,647 + +2.2 2,159,648 - +0.6 2,159,648 - +0.6 2,159,651 + +1.1 2,159,708 + -0.3 2,159,708 + +0.6 2,159,731 + +0.4 2,159,751 + +0.5 2,159,771 + +0.2 2,159,840 - -0.7 2,160,026 + CA265_RS08610 0.27 +0.5 2,160,050 + CA265_RS08610 0.29 +1.0 2,160,082 + CA265_RS08610 0.32 +1.4 2,160,083 - CA265_RS08610 0.32 -0.6 2,160,137 - CA265_RS08610 0.38 -0.1 2,160,238 + CA265_RS08610 0.47 +1.4 2,160,239 - CA265_RS08610 0.48 -0.5 2,160,273 - CA265_RS08610 0.51 +0.2 2,160,446 - CA265_RS08610 0.68 -1.2 2,160,446 - CA265_RS08610 0.68 -0.8 2,160,479 + CA265_RS08610 0.71 +0.8 2,160,488 - CA265_RS08610 0.72 -0.0 2,160,494 + CA265_RS08610 0.73 +0.3 2,160,544 + CA265_RS08610 0.77 -1.1 2,160,628 + CA265_RS08610 0.86 -1.5 2,160,629 - CA265_RS08610 0.86 +0.1 2,160,670 - CA265_RS08610 0.90 -0.6 2,160,670 - CA265_RS08610 0.90 +0.7
Or see this region's nucleotide sequence