Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07475 and CA265_RS07480 are separated by 2 nucleotides CA265_RS07480 and CA265_RS07485 are separated by 119 nucleotides CA265_RS07485 and CA265_RS07490 are separated by 77 nucleotides CA265_RS07490 and CA265_RS07495 are separated by 85 nucleotides
CA265_RS07475: CA265_RS07475 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,886,244 to 1,886,969
_RS07475
CA265_RS07480: CA265_RS07480 - aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C, at 1,886,972 to 1,887,262
_RS07480
CA265_RS07485: CA265_RS07485 - macrolide ABC transporter ATP-binding protein, at 1,887,382 to 1,888,140
_RS07485
CA265_RS07490: CA265_RS07490 - ATP:cob(I)alamin adenosyltransferase, at 1,888,218 to 1,888,766
_RS07490
CA265_RS07495: CA265_RS07495 - hypothetical protein, at 1,888,852 to 1,889,073
_RS07495
Position (kb)
1887
1888
1889 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1886.463 kb on + strand, within CA265_RS07475 at 1886.464 kb on - strand, within CA265_RS07475 at 1886.466 kb on - strand, within CA265_RS07475 at 1886.514 kb on - strand, within CA265_RS07475 at 1886.601 kb on - strand, within CA265_RS07475 at 1886.637 kb on - strand, within CA265_RS07475 at 1886.702 kb on + strand, within CA265_RS07475 at 1886.772 kb on - strand, within CA265_RS07475 at 1886.841 kb on + strand, within CA265_RS07475 at 1886.871 kb on + strand, within CA265_RS07475 at 1886.891 kb on + strand, within CA265_RS07475 at 1886.942 kb on + strand at 1886.957 kb on + strand at 1886.965 kb on + strand at 1886.965 kb on + strand at 1887.263 kb on + strand at 1887.269 kb on + strand at 1887.358 kb on - strand at 1887.424 kb on + strand at 1887.455 kb on + strand at 1887.474 kb on + strand, within CA265_RS07485 at 1887.475 kb on - strand, within CA265_RS07485 at 1887.477 kb on + strand, within CA265_RS07485 at 1887.478 kb on - strand, within CA265_RS07485 at 1887.540 kb on + strand, within CA265_RS07485 at 1887.545 kb on + strand, within CA265_RS07485 at 1887.558 kb on + strand, within CA265_RS07485 at 1887.578 kb on - strand, within CA265_RS07485 at 1887.637 kb on - strand, within CA265_RS07485 at 1887.700 kb on - strand, within CA265_RS07485 at 1887.705 kb on - strand, within CA265_RS07485 at 1887.716 kb on + strand, within CA265_RS07485 at 1887.770 kb on + strand, within CA265_RS07485 at 1887.785 kb on + strand, within CA265_RS07485 at 1887.785 kb on + strand, within CA265_RS07485 at 1887.804 kb on + strand, within CA265_RS07485 at 1887.805 kb on - strand, within CA265_RS07485 at 1887.868 kb on - strand, within CA265_RS07485 at 1887.880 kb on - strand, within CA265_RS07485 at 1887.884 kb on + strand, within CA265_RS07485 at 1887.908 kb on + strand, within CA265_RS07485 at 1887.908 kb on + strand, within CA265_RS07485 at 1887.908 kb on + strand, within CA265_RS07485 at 1887.908 kb on + strand, within CA265_RS07485 at 1887.970 kb on - strand, within CA265_RS07485 at 1887.999 kb on + strand, within CA265_RS07485 at 1888.000 kb on - strand, within CA265_RS07485 at 1888.025 kb on + strand, within CA265_RS07485 at 1888.025 kb on + strand, within CA265_RS07485 at 1888.062 kb on - strand, within CA265_RS07485 at 1888.065 kb on + strand at 1888.083 kb on - strand at 1888.110 kb on + strand at 1888.153 kb on - strand at 1888.287 kb on + strand, within CA265_RS07490 at 1888.345 kb on + strand, within CA265_RS07490 at 1888.345 kb on + strand, within CA265_RS07490 at 1888.361 kb on + strand, within CA265_RS07490 at 1888.413 kb on - strand, within CA265_RS07490 at 1888.413 kb on - strand, within CA265_RS07490 at 1888.413 kb on - strand, within CA265_RS07490 at 1888.413 kb on - strand, within CA265_RS07490 at 1888.644 kb on - strand, within CA265_RS07490 at 1888.664 kb on - strand, within CA265_RS07490 at 1888.671 kb on + strand, within CA265_RS07490 at 1888.692 kb on + strand, within CA265_RS07490 at 1888.692 kb on + strand, within CA265_RS07490 at 1888.693 kb on - strand, within CA265_RS07490 at 1888.693 kb on - strand, within CA265_RS07490 at 1888.696 kb on + strand, within CA265_RS07490 at 1888.768 kb on - strand at 1888.807 kb on + strand at 1888.822 kb on + strand at 1888.836 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 1,886,463 + CA265_RS07475 0.30 -0.8 1,886,464 - CA265_RS07475 0.30 +0.8 1,886,466 - CA265_RS07475 0.31 -0.3 1,886,514 - CA265_RS07475 0.37 +1.2 1,886,601 - CA265_RS07475 0.49 +0.4 1,886,637 - CA265_RS07475 0.54 -0.0 1,886,702 + CA265_RS07475 0.63 -2.6 1,886,772 - CA265_RS07475 0.73 -0.9 1,886,841 + CA265_RS07475 0.82 +2.0 1,886,871 + CA265_RS07475 0.86 +0.5 1,886,891 + CA265_RS07475 0.89 +1.0 1,886,942 + -0.0 1,886,957 + +0.8 1,886,965 + -0.4 1,886,965 + -0.1 1,887,263 + -2.0 1,887,269 + +0.1 1,887,358 - +0.2 1,887,424 + -0.5 1,887,455 + -0.1 1,887,474 + CA265_RS07485 0.12 -0.7 1,887,475 - CA265_RS07485 0.12 -0.1 1,887,477 + CA265_RS07485 0.13 +0.3 1,887,478 - CA265_RS07485 0.13 +0.7 1,887,540 + CA265_RS07485 0.21 -0.6 1,887,545 + CA265_RS07485 0.21 -0.8 1,887,558 + CA265_RS07485 0.23 +0.4 1,887,578 - CA265_RS07485 0.26 +0.7 1,887,637 - CA265_RS07485 0.34 -2.0 1,887,700 - CA265_RS07485 0.42 +0.4 1,887,705 - CA265_RS07485 0.43 +0.8 1,887,716 + CA265_RS07485 0.44 -0.2 1,887,770 + CA265_RS07485 0.51 +1.0 1,887,785 + CA265_RS07485 0.53 +0.1 1,887,785 + CA265_RS07485 0.53 +1.0 1,887,804 + CA265_RS07485 0.56 +1.7 1,887,805 - CA265_RS07485 0.56 +0.8 1,887,868 - CA265_RS07485 0.64 -0.1 1,887,880 - CA265_RS07485 0.66 -0.1 1,887,884 + CA265_RS07485 0.66 +1.3 1,887,908 + CA265_RS07485 0.69 -0.8 1,887,908 + CA265_RS07485 0.69 +2.2 1,887,908 + CA265_RS07485 0.69 -0.4 1,887,908 + CA265_RS07485 0.69 +0.4 1,887,970 - CA265_RS07485 0.77 +1.5 1,887,999 + CA265_RS07485 0.81 +0.7 1,888,000 - CA265_RS07485 0.81 -0.0 1,888,025 + CA265_RS07485 0.85 +1.0 1,888,025 + CA265_RS07485 0.85 +0.2 1,888,062 - CA265_RS07485 0.90 +0.5 1,888,065 + +1.0 1,888,083 - -1.3 1,888,110 + +0.6 1,888,153 - -0.5 1,888,287 + CA265_RS07490 0.13 +1.2 1,888,345 + CA265_RS07490 0.23 +0.4 1,888,345 + CA265_RS07490 0.23 +1.1 1,888,361 + CA265_RS07490 0.26 +0.8 1,888,413 - CA265_RS07490 0.36 +1.1 1,888,413 - CA265_RS07490 0.36 +1.3 1,888,413 - CA265_RS07490 0.36 +0.4 1,888,413 - CA265_RS07490 0.36 +1.6 1,888,644 - CA265_RS07490 0.78 -0.4 1,888,664 - CA265_RS07490 0.81 -0.3 1,888,671 + CA265_RS07490 0.83 +2.0 1,888,692 + CA265_RS07490 0.86 +0.7 1,888,692 + CA265_RS07490 0.86 +0.4 1,888,693 - CA265_RS07490 0.87 +0.3 1,888,693 - CA265_RS07490 0.87 +0.4 1,888,696 + CA265_RS07490 0.87 +0.7 1,888,768 - +0.9 1,888,807 + +0.0 1,888,822 + +0.3 1,888,836 + +0.3
Or see this region's nucleotide sequence