Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05085 and CA265_RS05090 are separated by 709 nucleotides CA265_RS05090 and CA265_RS05095 are separated by 249 nucleotides CA265_RS05095 and CA265_RS05100 are separated by 451 nucleotides
CA265_RS05085: CA265_RS05085 - hypothetical protein, at 1,207,676 to 1,213,990
_RS05085
CA265_RS05090: CA265_RS05090 - hypothetical protein, at 1,214,700 to 1,214,954
_RS05090
CA265_RS05095: CA265_RS05095 - hypothetical protein, at 1,215,204 to 1,215,413
_RS05095
CA265_RS05100: CA265_RS05100 - threonylcarbamoyl-AMP synthase, at 1,215,865 to 1,216,485
_RS05100
Position (kb)
1214
1215 Strain fitness (log2 ratio)
-1
0
1
2 at 1213.712 kb on + strand at 1213.750 kb on - strand at 1213.750 kb on - strand at 1213.780 kb on - strand at 1213.857 kb on - strand at 1213.857 kb on - strand at 1213.993 kb on - strand at 1213.995 kb on + strand at 1213.995 kb on + strand at 1214.086 kb on + strand at 1214.116 kb on + strand at 1214.248 kb on + strand at 1214.260 kb on + strand at 1214.265 kb on + strand at 1214.324 kb on - strand at 1214.418 kb on + strand at 1214.419 kb on - strand at 1214.476 kb on + strand at 1214.571 kb on - strand at 1214.659 kb on - strand at 1214.661 kb on + strand at 1214.694 kb on - strand at 1214.816 kb on + strand, within CA265_RS05090 at 1214.817 kb on - strand, within CA265_RS05090 at 1214.828 kb on - strand, within CA265_RS05090 at 1214.923 kb on + strand, within CA265_RS05090 at 1214.958 kb on - strand at 1214.958 kb on - strand at 1215.090 kb on - strand at 1215.117 kb on + strand at 1215.118 kb on - strand at 1215.129 kb on + strand at 1215.160 kb on + strand at 1215.166 kb on - strand at 1215.559 kb on - strand at 1215.600 kb on + strand at 1215.612 kb on + strand at 1215.613 kb on - strand at 1215.613 kb on - strand at 1215.613 kb on - strand at 1215.654 kb on + strand at 1215.655 kb on - strand at 1215.658 kb on - strand at 1215.699 kb on + strand at 1215.758 kb on + strand at 1215.759 kb on - strand at 1215.821 kb on - strand at 1215.853 kb on + strand at 1215.866 kb on + strand at 1215.882 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 1,213,712 + +0.5 1,213,750 - -0.4 1,213,750 - -0.5 1,213,780 - -0.1 1,213,857 - +0.2 1,213,857 - -0.3 1,213,993 - -0.4 1,213,995 + +0.2 1,213,995 + -1.5 1,214,086 + +1.3 1,214,116 + +0.9 1,214,248 + -0.4 1,214,260 + +1.4 1,214,265 + -0.5 1,214,324 - +0.2 1,214,418 + -0.1 1,214,419 - +0.6 1,214,476 + -0.9 1,214,571 - +0.6 1,214,659 - -0.7 1,214,661 + +0.7 1,214,694 - -0.1 1,214,816 + CA265_RS05090 0.45 +0.6 1,214,817 - CA265_RS05090 0.46 +1.7 1,214,828 - CA265_RS05090 0.50 +1.2 1,214,923 + CA265_RS05090 0.87 +1.4 1,214,958 - +0.7 1,214,958 - -0.1 1,215,090 - +0.1 1,215,117 + -1.1 1,215,118 - -0.4 1,215,129 + +0.7 1,215,160 + +0.7 1,215,166 - +1.2 1,215,559 - -0.1 1,215,600 + +0.3 1,215,612 + -0.5 1,215,613 - +0.5 1,215,613 - -0.0 1,215,613 - -1.0 1,215,654 + +0.7 1,215,655 - +2.2 1,215,658 - +1.9 1,215,699 + +0.5 1,215,758 + -1.7 1,215,759 - +1.6 1,215,821 - -0.2 1,215,853 + -0.9 1,215,866 + -1.0 1,215,882 + -1.5
Or see this region's nucleotide sequence