Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04175 and CA265_RS04180 are separated by 217 nucleotides CA265_RS04180 and CA265_RS04185 are separated by 77 nucleotides
CA265_RS04175: CA265_RS04175 - phosphonate ABC transporter ATP-binding protein, at 1,008,619 to 1,009,317
_RS04175
CA265_RS04180: CA265_RS04180 - cell division protein FtsX, at 1,009,535 to 1,011,904
_RS04180
CA265_RS04185: CA265_RS04185 - hypothetical protein, at 1,011,982 to 1,014,357
_RS04185
Position (kb)
1009
1010
1011
1012 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1008.552 kb on - strand at 1008.565 kb on - strand at 1008.608 kb on + strand at 1008.803 kb on - strand, within CA265_RS04175 at 1009.008 kb on + strand, within CA265_RS04175 at 1009.067 kb on + strand, within CA265_RS04175 at 1009.138 kb on - strand, within CA265_RS04175 at 1009.150 kb on - strand, within CA265_RS04175 at 1009.157 kb on + strand, within CA265_RS04175 at 1009.157 kb on + strand, within CA265_RS04175 at 1009.158 kb on - strand, within CA265_RS04175 at 1009.179 kb on - strand, within CA265_RS04175 at 1009.312 kb on - strand at 1009.316 kb on - strand at 1009.412 kb on + strand at 1009.413 kb on - strand at 1009.413 kb on - strand at 1009.413 kb on - strand at 1009.419 kb on + strand at 1009.471 kb on - strand at 1009.502 kb on + strand at 1009.535 kb on - strand at 1009.535 kb on - strand at 1009.648 kb on - strand at 1009.651 kb on - strand at 1009.652 kb on + strand at 1009.662 kb on - strand at 1009.699 kb on + strand at 1009.738 kb on + strand at 1009.807 kb on + strand, within CA265_RS04180 at 1009.807 kb on + strand, within CA265_RS04180 at 1009.829 kb on + strand, within CA265_RS04180 at 1009.830 kb on - strand, within CA265_RS04180 at 1009.830 kb on - strand, within CA265_RS04180 at 1009.874 kb on - strand, within CA265_RS04180 at 1009.911 kb on + strand, within CA265_RS04180 at 1009.962 kb on + strand, within CA265_RS04180 at 1009.966 kb on + strand, within CA265_RS04180 at 1009.966 kb on + strand, within CA265_RS04180 at 1009.966 kb on + strand, within CA265_RS04180 at 1010.026 kb on - strand, within CA265_RS04180 at 1010.058 kb on + strand, within CA265_RS04180 at 1010.059 kb on - strand, within CA265_RS04180 at 1010.077 kb on - strand, within CA265_RS04180 at 1010.095 kb on + strand, within CA265_RS04180 at 1010.096 kb on - strand, within CA265_RS04180 at 1010.116 kb on + strand, within CA265_RS04180 at 1010.116 kb on + strand, within CA265_RS04180 at 1010.116 kb on + strand, within CA265_RS04180 at 1010.117 kb on - strand, within CA265_RS04180 at 1010.117 kb on - strand, within CA265_RS04180 at 1010.117 kb on - strand, within CA265_RS04180 at 1010.119 kb on + strand, within CA265_RS04180 at 1010.179 kb on + strand, within CA265_RS04180 at 1010.205 kb on + strand, within CA265_RS04180 at 1010.251 kb on + strand, within CA265_RS04180 at 1010.251 kb on + strand, within CA265_RS04180 at 1010.252 kb on - strand, within CA265_RS04180 at 1010.406 kb on + strand, within CA265_RS04180 at 1010.512 kb on + strand, within CA265_RS04180 at 1010.574 kb on + strand, within CA265_RS04180 at 1010.590 kb on - strand, within CA265_RS04180 at 1010.698 kb on + strand, within CA265_RS04180 at 1010.714 kb on + strand, within CA265_RS04180 at 1010.715 kb on - strand, within CA265_RS04180 at 1010.718 kb on + strand, within CA265_RS04180 at 1010.863 kb on + strand, within CA265_RS04180 at 1010.922 kb on + strand, within CA265_RS04180 at 1010.923 kb on - strand, within CA265_RS04180 at 1010.923 kb on - strand, within CA265_RS04180 at 1010.952 kb on + strand, within CA265_RS04180 at 1010.974 kb on + strand, within CA265_RS04180 at 1010.975 kb on - strand, within CA265_RS04180 at 1010.975 kb on - strand, within CA265_RS04180 at 1011.040 kb on + strand, within CA265_RS04180 at 1011.040 kb on + strand, within CA265_RS04180 at 1011.040 kb on + strand, within CA265_RS04180 at 1011.040 kb on + strand, within CA265_RS04180 at 1011.105 kb on + strand, within CA265_RS04180 at 1011.105 kb on + strand, within CA265_RS04180 at 1011.106 kb on - strand, within CA265_RS04180 at 1011.106 kb on - strand, within CA265_RS04180 at 1011.106 kb on - strand, within CA265_RS04180 at 1011.142 kb on - strand, within CA265_RS04180 at 1011.145 kb on - strand, within CA265_RS04180 at 1011.192 kb on + strand, within CA265_RS04180 at 1011.192 kb on + strand, within CA265_RS04180 at 1011.274 kb on - strand, within CA265_RS04180 at 1011.277 kb on + strand, within CA265_RS04180 at 1011.277 kb on + strand, within CA265_RS04180 at 1011.278 kb on - strand, within CA265_RS04180 at 1011.287 kb on - strand, within CA265_RS04180 at 1011.291 kb on + strand, within CA265_RS04180 at 1011.436 kb on - strand, within CA265_RS04180 at 1011.436 kb on - strand, within CA265_RS04180 at 1011.454 kb on + strand, within CA265_RS04180 at 1011.454 kb on + strand, within CA265_RS04180 at 1011.454 kb on + strand, within CA265_RS04180 at 1011.526 kb on + strand, within CA265_RS04180 at 1011.629 kb on - strand, within CA265_RS04180 at 1011.662 kb on - strand, within CA265_RS04180 at 1011.680 kb on - strand at 1011.715 kb on + strand at 1011.716 kb on - strand at 1011.734 kb on + strand at 1011.745 kb on - strand at 1011.835 kb on - strand at 1011.881 kb on - strand at 1011.955 kb on + strand at 1011.961 kb on - strand at 1011.981 kb on + strand at 1011.981 kb on + strand at 1011.981 kb on + strand at 1011.982 kb on - strand at 1011.982 kb on - strand at 1012.084 kb on - strand at 1012.093 kb on - strand at 1012.099 kb on + strand at 1012.104 kb on + strand at 1012.109 kb on - strand at 1012.109 kb on - strand at 1012.142 kb on - strand at 1012.142 kb on - strand at 1012.242 kb on - strand, within CA265_RS04185 at 1012.297 kb on - strand, within CA265_RS04185 at 1012.317 kb on + strand, within CA265_RS04185 at 1012.318 kb on - strand, within CA265_RS04185 at 1012.336 kb on + strand, within CA265_RS04185 at 1012.395 kb on + strand, within CA265_RS04185 at 1012.503 kb on + strand, within CA265_RS04185 at 1012.504 kb on - strand, within CA265_RS04185 at 1012.506 kb on - strand, within CA265_RS04185 at 1012.527 kb on + strand, within CA265_RS04185 at 1012.548 kb on + strand, within CA265_RS04185 at 1012.610 kb on - strand, within CA265_RS04185 at 1012.611 kb on + strand, within CA265_RS04185 at 1012.618 kb on + strand, within CA265_RS04185 at 1012.620 kb on + strand, within CA265_RS04185 at 1012.621 kb on - strand, within CA265_RS04185 at 1012.623 kb on + strand, within CA265_RS04185 at 1012.624 kb on - strand, within CA265_RS04185 at 1012.624 kb on - strand, within CA265_RS04185 at 1012.628 kb on - strand, within CA265_RS04185 at 1012.688 kb on - strand, within CA265_RS04185 at 1012.811 kb on - strand, within CA265_RS04185 at 1012.842 kb on + strand at 1012.847 kb on + strand, within CA265_RS04185 at 1012.848 kb on - strand, within CA265_RS04185 at 1012.888 kb on - strand, within CA265_RS04185 at 1012.890 kb on - strand, within CA265_RS04185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 1,008,552 - -0.1 1,008,565 - -0.2 1,008,608 + -1.9 1,008,803 - CA265_RS04175 0.26 -0.1 1,009,008 + CA265_RS04175 0.56 -0.7 1,009,067 + CA265_RS04175 0.64 +0.1 1,009,138 - CA265_RS04175 0.74 +0.3 1,009,150 - CA265_RS04175 0.76 -0.0 1,009,157 + CA265_RS04175 0.77 -2.0 1,009,157 + CA265_RS04175 0.77 -2.1 1,009,158 - CA265_RS04175 0.77 +0.7 1,009,179 - CA265_RS04175 0.80 -0.4 1,009,312 - -0.3 1,009,316 - +0.6 1,009,412 + -3.3 1,009,413 - +0.3 1,009,413 - -0.7 1,009,413 - -0.4 1,009,419 + +0.3 1,009,471 - +0.7 1,009,502 + -3.1 1,009,535 - -0.1 1,009,535 - +1.4 1,009,648 - +0.4 1,009,651 - +0.4 1,009,652 + +0.3 1,009,662 - +0.5 1,009,699 + -3.5 1,009,738 + -2.9 1,009,807 + CA265_RS04180 0.11 -1.0 1,009,807 + CA265_RS04180 0.11 -0.1 1,009,829 + CA265_RS04180 0.12 -2.3 1,009,830 - CA265_RS04180 0.12 +0.1 1,009,830 - CA265_RS04180 0.12 +0.1 1,009,874 - CA265_RS04180 0.14 +0.1 1,009,911 + CA265_RS04180 0.16 -1.8 1,009,962 + CA265_RS04180 0.18 -1.6 1,009,966 + CA265_RS04180 0.18 -1.1 1,009,966 + CA265_RS04180 0.18 -1.4 1,009,966 + CA265_RS04180 0.18 -1.3 1,010,026 - CA265_RS04180 0.21 +0.7 1,010,058 + CA265_RS04180 0.22 -0.8 1,010,059 - CA265_RS04180 0.22 -0.9 1,010,077 - CA265_RS04180 0.23 -1.4 1,010,095 + CA265_RS04180 0.24 -1.5 1,010,096 - CA265_RS04180 0.24 +0.1 1,010,116 + CA265_RS04180 0.25 -3.9 1,010,116 + CA265_RS04180 0.25 -1.8 1,010,116 + CA265_RS04180 0.25 -0.9 1,010,117 - CA265_RS04180 0.25 +0.9 1,010,117 - CA265_RS04180 0.25 -1.6 1,010,117 - CA265_RS04180 0.25 -0.1 1,010,119 + CA265_RS04180 0.25 -4.1 1,010,179 + CA265_RS04180 0.27 +0.1 1,010,205 + CA265_RS04180 0.28 -0.3 1,010,251 + CA265_RS04180 0.30 -0.7 1,010,251 + CA265_RS04180 0.30 -1.3 1,010,252 - CA265_RS04180 0.30 -1.1 1,010,406 + CA265_RS04180 0.37 -1.9 1,010,512 + CA265_RS04180 0.41 -0.2 1,010,574 + CA265_RS04180 0.44 -0.7 1,010,590 - CA265_RS04180 0.45 +0.4 1,010,698 + CA265_RS04180 0.49 -1.4 1,010,714 + CA265_RS04180 0.50 -0.5 1,010,715 - CA265_RS04180 0.50 +1.2 1,010,718 + CA265_RS04180 0.50 -1.3 1,010,863 + CA265_RS04180 0.56 -1.6 1,010,922 + CA265_RS04180 0.59 -1.2 1,010,923 - CA265_RS04180 0.59 -0.4 1,010,923 - CA265_RS04180 0.59 -0.5 1,010,952 + CA265_RS04180 0.60 -2.1 1,010,974 + CA265_RS04180 0.61 +0.0 1,010,975 - CA265_RS04180 0.61 -1.3 1,010,975 - CA265_RS04180 0.61 +0.9 1,011,040 + CA265_RS04180 0.64 -1.0 1,011,040 + CA265_RS04180 0.64 -2.0 1,011,040 + CA265_RS04180 0.64 -3.2 1,011,040 + CA265_RS04180 0.64 -1.6 1,011,105 + CA265_RS04180 0.66 +0.4 1,011,105 + CA265_RS04180 0.66 -1.6 1,011,106 - CA265_RS04180 0.66 -0.6 1,011,106 - CA265_RS04180 0.66 +1.2 1,011,106 - CA265_RS04180 0.66 -0.6 1,011,142 - CA265_RS04180 0.68 -1.2 1,011,145 - CA265_RS04180 0.68 +0.6 1,011,192 + CA265_RS04180 0.70 -1.4 1,011,192 + CA265_RS04180 0.70 -1.7 1,011,274 - CA265_RS04180 0.73 -0.0 1,011,277 + CA265_RS04180 0.74 -2.6 1,011,277 + CA265_RS04180 0.74 -2.5 1,011,278 - CA265_RS04180 0.74 -1.4 1,011,287 - CA265_RS04180 0.74 -1.5 1,011,291 + CA265_RS04180 0.74 -1.6 1,011,436 - CA265_RS04180 0.80 -0.3 1,011,436 - CA265_RS04180 0.80 -0.9 1,011,454 + CA265_RS04180 0.81 -0.6 1,011,454 + CA265_RS04180 0.81 +0.6 1,011,454 + CA265_RS04180 0.81 -1.3 1,011,526 + CA265_RS04180 0.84 -3.0 1,011,629 - CA265_RS04180 0.88 +1.2 1,011,662 - CA265_RS04180 0.90 +0.5 1,011,680 - -0.3 1,011,715 + -2.1 1,011,716 - -0.2 1,011,734 + -0.7 1,011,745 - -1.0 1,011,835 - -0.1 1,011,881 - -0.3 1,011,955 + +1.5 1,011,961 - +0.4 1,011,981 + +0.6 1,011,981 + -2.7 1,011,981 + -0.3 1,011,982 - +1.6 1,011,982 - -1.2 1,012,084 - +1.1 1,012,093 - +1.2 1,012,099 + -1.0 1,012,104 + -0.8 1,012,109 - -1.0 1,012,109 - -1.9 1,012,142 - +2.3 1,012,142 - -0.4 1,012,242 - CA265_RS04185 0.11 -0.7 1,012,297 - CA265_RS04185 0.13 -0.6 1,012,317 + CA265_RS04185 0.14 -2.0 1,012,318 - CA265_RS04185 0.14 +0.9 1,012,336 + CA265_RS04185 0.15 -1.4 1,012,395 + CA265_RS04185 0.17 -0.9 1,012,503 + CA265_RS04185 0.22 -0.8 1,012,504 - CA265_RS04185 0.22 +1.5 1,012,506 - CA265_RS04185 0.22 +0.7 1,012,527 + CA265_RS04185 0.23 -0.6 1,012,548 + CA265_RS04185 0.24 +0.5 1,012,610 - CA265_RS04185 0.26 +0.3 1,012,611 + CA265_RS04185 0.26 -0.1 1,012,618 + CA265_RS04185 0.27 -0.8 1,012,620 + CA265_RS04185 0.27 -1.0 1,012,621 - CA265_RS04185 0.27 -0.1 1,012,623 + CA265_RS04185 0.27 -0.2 1,012,624 - CA265_RS04185 0.27 +0.3 1,012,624 - CA265_RS04185 0.27 +0.7 1,012,628 - CA265_RS04185 0.27 -0.2 1,012,688 - CA265_RS04185 0.30 +0.2 1,012,811 - CA265_RS04185 0.35 +2.6 1,012,842 + +0.3 1,012,847 + CA265_RS04185 0.36 +0.2 1,012,848 - CA265_RS04185 0.36 +0.5 1,012,888 - CA265_RS04185 0.38 +0.5 1,012,890 - CA265_RS04185 0.38 +0.1
Or see this region's nucleotide sequence