Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04080 and CA265_RS04085 are separated by 137 nucleotides CA265_RS04085 and CA265_RS04090 are separated by 215 nucleotides
CA265_RS04080: CA265_RS04080 - hypothetical protein, at 980,016 to 982,451
_RS04080
CA265_RS04085: CA265_RS04085 - phosphohydrolase, at 982,589 to 983,809
_RS04085
CA265_RS04090: CA265_RS04090 - transcriptional regulator, at 984,025 to 984,471
_RS04090
Position (kb) 982
983
984 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 981.617 kb on + strand, within CA265_RS04080 at 981.645 kb on + strand, within CA265_RS04080 at 981.646 kb on - strand, within CA265_RS04080 at 981.751 kb on - strand, within CA265_RS04080 at 981.771 kb on - strand, within CA265_RS04080 at 981.852 kb on + strand, within CA265_RS04080 at 981.883 kb on + strand, within CA265_RS04080 at 981.883 kb on + strand, within CA265_RS04080 at 981.888 kb on - strand, within CA265_RS04080 at 981.898 kb on + strand, within CA265_RS04080 at 981.984 kb on - strand, within CA265_RS04080 at 981.984 kb on - strand, within CA265_RS04080 at 981.985 kb on + strand, within CA265_RS04080 at 981.985 kb on + strand, within CA265_RS04080 at 981.986 kb on - strand, within CA265_RS04080 at 981.986 kb on - strand, within CA265_RS04080 at 982.051 kb on - strand, within CA265_RS04080 at 982.093 kb on + strand, within CA265_RS04080 at 982.103 kb on - strand, within CA265_RS04080 at 982.103 kb on - strand, within CA265_RS04080 at 982.103 kb on - strand, within CA265_RS04080 at 982.103 kb on - strand, within CA265_RS04080 at 982.135 kb on - strand, within CA265_RS04080 at 982.165 kb on + strand, within CA265_RS04080 at 982.265 kb on - strand at 982.333 kb on - strand at 982.368 kb on + strand at 982.423 kb on - strand at 982.442 kb on + strand at 982.482 kb on + strand at 982.505 kb on + strand at 982.505 kb on + strand at 982.509 kb on + strand at 982.578 kb on + strand at 982.578 kb on + strand at 982.610 kb on + strand at 982.611 kb on - strand at 982.683 kb on + strand at 982.703 kb on - strand at 982.734 kb on - strand, within CA265_RS04085 at 983.035 kb on - strand, within CA265_RS04085 at 983.090 kb on - strand, within CA265_RS04085 at 983.091 kb on + strand, within CA265_RS04085 at 983.091 kb on + strand, within CA265_RS04085 at 983.101 kb on - strand, within CA265_RS04085 at 983.167 kb on - strand, within CA265_RS04085 at 983.167 kb on - strand, within CA265_RS04085 at 983.295 kb on - strand, within CA265_RS04085 at 983.353 kb on - strand, within CA265_RS04085 at 983.359 kb on + strand, within CA265_RS04085 at 983.360 kb on - strand, within CA265_RS04085 at 983.365 kb on + strand, within CA265_RS04085 at 983.383 kb on - strand, within CA265_RS04085 at 983.396 kb on - strand, within CA265_RS04085 at 983.411 kb on + strand, within CA265_RS04085 at 983.457 kb on + strand, within CA265_RS04085 at 983.516 kb on + strand, within CA265_RS04085 at 983.517 kb on - strand, within CA265_RS04085 at 983.558 kb on + strand, within CA265_RS04085 at 983.558 kb on + strand, within CA265_RS04085 at 983.559 kb on - strand, within CA265_RS04085 at 983.650 kb on - strand, within CA265_RS04085 at 983.678 kb on - strand, within CA265_RS04085 at 983.678 kb on - strand, within CA265_RS04085 at 983.699 kb on - strand at 983.708 kb on - strand at 983.836 kb on - strand at 983.866 kb on - strand at 983.866 kb on - strand at 983.870 kb on + strand at 983.919 kb on - strand at 983.936 kb on + strand at 983.947 kb on - strand at 983.947 kb on - strand at 983.985 kb on + strand at 983.993 kb on - strand at 984.088 kb on - strand, within CA265_RS04090 at 984.388 kb on - strand, within CA265_RS04090 at 984.460 kb on - strand at 984.524 kb on + strand at 984.529 kb on + strand at 984.561 kb on - strand at 984.624 kb on + strand at 984.678 kb on - strand at 984.709 kb on - strand at 984.762 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 981,617 + CA265_RS04080 0.66 +0.7 981,645 + CA265_RS04080 0.67 +0.1 981,646 - CA265_RS04080 0.67 -1.7 981,751 - CA265_RS04080 0.71 -0.8 981,771 - CA265_RS04080 0.72 +0.3 981,852 + CA265_RS04080 0.75 -0.3 981,883 + CA265_RS04080 0.77 +0.2 981,883 + CA265_RS04080 0.77 +0.3 981,888 - CA265_RS04080 0.77 -0.4 981,898 + CA265_RS04080 0.77 +0.3 981,984 - CA265_RS04080 0.81 -0.5 981,984 - CA265_RS04080 0.81 -1.7 981,985 + CA265_RS04080 0.81 +0.2 981,985 + CA265_RS04080 0.81 -0.2 981,986 - CA265_RS04080 0.81 -1.9 981,986 - CA265_RS04080 0.81 -0.2 982,051 - CA265_RS04080 0.84 -2.0 982,093 + CA265_RS04080 0.85 +1.4 982,103 - CA265_RS04080 0.86 -0.7 982,103 - CA265_RS04080 0.86 +0.1 982,103 - CA265_RS04080 0.86 -1.1 982,103 - CA265_RS04080 0.86 +0.9 982,135 - CA265_RS04080 0.87 -0.9 982,165 + CA265_RS04080 0.88 -0.1 982,265 - -1.1 982,333 - -1.3 982,368 + +0.9 982,423 - -0.3 982,442 + -0.2 982,482 + -0.7 982,505 + -0.8 982,505 + +0.1 982,509 + -3.0 982,578 + -0.9 982,578 + -0.6 982,610 + -1.3 982,611 - -0.5 982,683 + +0.1 982,703 - +1.6 982,734 - CA265_RS04085 0.12 +0.4 983,035 - CA265_RS04085 0.37 +1.6 983,090 - CA265_RS04085 0.41 +0.4 983,091 + CA265_RS04085 0.41 +0.6 983,091 + CA265_RS04085 0.41 -1.3 983,101 - CA265_RS04085 0.42 +0.0 983,167 - CA265_RS04085 0.47 +0.1 983,167 - CA265_RS04085 0.47 +0.5 983,295 - CA265_RS04085 0.58 -0.1 983,353 - CA265_RS04085 0.63 -0.1 983,359 + CA265_RS04085 0.63 -1.0 983,360 - CA265_RS04085 0.63 +1.9 983,365 + CA265_RS04085 0.64 +0.3 983,383 - CA265_RS04085 0.65 +0.5 983,396 - CA265_RS04085 0.66 -0.5 983,411 + CA265_RS04085 0.67 +2.6 983,457 + CA265_RS04085 0.71 -0.2 983,516 + CA265_RS04085 0.76 +0.7 983,517 - CA265_RS04085 0.76 +0.9 983,558 + CA265_RS04085 0.79 +0.2 983,558 + CA265_RS04085 0.79 -0.1 983,559 - CA265_RS04085 0.79 +1.7 983,650 - CA265_RS04085 0.87 +0.1 983,678 - CA265_RS04085 0.89 +1.9 983,678 - CA265_RS04085 0.89 +1.3 983,699 - +0.2 983,708 - -1.6 983,836 - -1.1 983,866 - -1.6 983,866 - -0.7 983,870 + -1.2 983,919 - -4.2 983,936 + +1.3 983,947 - -1.9 983,947 - -3.6 983,985 + +1.0 983,993 - -2.0 984,088 - CA265_RS04090 0.14 -0.8 984,388 - CA265_RS04090 0.81 -0.4 984,460 - -1.2 984,524 + +1.1 984,529 + -0.3 984,561 - +0.7 984,624 + +0.5 984,678 - -0.4 984,709 - -0.0 984,762 + +0.0
Or see this region's nucleotide sequence