Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03100 and CA265_RS03105 are separated by 31 nucleotides CA265_RS03105 and CA265_RS03110 are separated by 244 nucleotides CA265_RS03110 and CA265_RS03115 are separated by 68 nucleotides CA265_RS03115 and CA265_RS03120 are separated by 3 nucleotides
CA265_RS03100: CA265_RS03100 - glucose dehydrogenase, at 769,370 to 770,581
_RS03100
CA265_RS03105: CA265_RS03105 - hypothetical protein, at 770,613 to 770,792
_RS03105
CA265_RS03110: CA265_RS03110 - DUF58 domain-containing protein, at 771,037 to 771,954
_RS03110
CA265_RS03115: CA265_RS03115 - peroxiredoxin, at 772,023 to 772,484
_RS03115
CA265_RS03120: CA265_RS03120 - disulfide bond formation protein DsbA, at 772,488 to 773,006
_RS03120
Position (kb) 771
772 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 770.103 kb on - strand, within CA265_RS03100 at 770.109 kb on - strand, within CA265_RS03100 at 770.116 kb on - strand, within CA265_RS03100 at 770.140 kb on + strand, within CA265_RS03100 at 770.247 kb on - strand, within CA265_RS03100 at 770.258 kb on + strand, within CA265_RS03100 at 770.260 kb on + strand, within CA265_RS03100 at 770.266 kb on + strand, within CA265_RS03100 at 770.351 kb on - strand, within CA265_RS03100 at 770.440 kb on - strand, within CA265_RS03100 at 770.453 kb on - strand, within CA265_RS03100 at 770.512 kb on - strand at 770.589 kb on - strand at 770.589 kb on - strand at 770.595 kb on - strand at 770.618 kb on + strand at 770.760 kb on - strand, within CA265_RS03105 at 770.795 kb on - strand at 770.853 kb on + strand at 770.853 kb on + strand at 770.854 kb on - strand at 770.908 kb on + strand at 770.954 kb on - strand at 770.969 kb on - strand at 771.025 kb on + strand at 771.025 kb on + strand at 771.052 kb on + strand at 771.101 kb on + strand at 771.101 kb on + strand at 771.104 kb on + strand at 771.180 kb on + strand, within CA265_RS03110 at 771.180 kb on + strand, within CA265_RS03110 at 771.183 kb on + strand, within CA265_RS03110 at 771.311 kb on - strand, within CA265_RS03110 at 771.373 kb on + strand, within CA265_RS03110 at 771.374 kb on - strand, within CA265_RS03110 at 771.417 kb on + strand, within CA265_RS03110 at 771.417 kb on + strand, within CA265_RS03110 at 771.473 kb on - strand, within CA265_RS03110 at 771.473 kb on - strand, within CA265_RS03110 at 771.476 kb on + strand, within CA265_RS03110 at 771.488 kb on + strand, within CA265_RS03110 at 771.510 kb on + strand, within CA265_RS03110 at 771.511 kb on - strand, within CA265_RS03110 at 771.516 kb on - strand, within CA265_RS03110 at 771.571 kb on - strand, within CA265_RS03110 at 771.602 kb on + strand, within CA265_RS03110 at 771.622 kb on - strand, within CA265_RS03110 at 771.622 kb on - strand, within CA265_RS03110 at 771.705 kb on + strand, within CA265_RS03110 at 771.781 kb on - strand, within CA265_RS03110 at 771.852 kb on - strand, within CA265_RS03110 at 771.866 kb on - strand at 771.868 kb on - strand at 771.925 kb on + strand at 772.022 kb on + strand at 772.022 kb on + strand at 772.022 kb on + strand at 772.042 kb on + strand at 772.042 kb on + strand at 772.204 kb on - strand, within CA265_RS03115 at 772.329 kb on + strand, within CA265_RS03115 at 772.361 kb on + strand, within CA265_RS03115 at 772.454 kb on - strand at 772.488 kb on - strand at 772.553 kb on - strand, within CA265_RS03120 at 772.588 kb on - strand, within CA265_RS03120 at 772.610 kb on + strand, within CA265_RS03120 at 772.736 kb on + strand, within CA265_RS03120 at 772.826 kb on - strand, within CA265_RS03120 at 772.869 kb on - strand, within CA265_RS03120 at 772.879 kb on + strand, within CA265_RS03120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 770,103 - CA265_RS03100 0.60 +0.6 770,109 - CA265_RS03100 0.61 +0.6 770,116 - CA265_RS03100 0.62 +0.5 770,140 + CA265_RS03100 0.64 -0.2 770,247 - CA265_RS03100 0.72 -0.4 770,258 + CA265_RS03100 0.73 +0.9 770,260 + CA265_RS03100 0.73 -0.3 770,266 + CA265_RS03100 0.74 +0.6 770,351 - CA265_RS03100 0.81 -1.0 770,440 - CA265_RS03100 0.88 -0.4 770,453 - CA265_RS03100 0.89 -0.8 770,512 - +0.2 770,589 - -0.6 770,589 - -0.7 770,595 - -0.2 770,618 + -1.0 770,760 - CA265_RS03105 0.82 +0.1 770,795 - -0.4 770,853 + -0.9 770,853 + -0.2 770,854 - -0.0 770,908 + -0.4 770,954 - -0.0 770,969 - +0.4 771,025 + -0.4 771,025 + +0.3 771,052 + +1.6 771,101 + +1.3 771,101 + +0.6 771,104 + +0.3 771,180 + CA265_RS03110 0.16 -0.5 771,180 + CA265_RS03110 0.16 +1.1 771,183 + CA265_RS03110 0.16 -0.4 771,311 - CA265_RS03110 0.30 -0.5 771,373 + CA265_RS03110 0.37 +0.7 771,374 - CA265_RS03110 0.37 +1.1 771,417 + CA265_RS03110 0.41 +0.9 771,417 + CA265_RS03110 0.41 +0.8 771,473 - CA265_RS03110 0.47 -0.2 771,473 - CA265_RS03110 0.47 +0.6 771,476 + CA265_RS03110 0.48 +1.2 771,488 + CA265_RS03110 0.49 +0.8 771,510 + CA265_RS03110 0.52 +1.1 771,511 - CA265_RS03110 0.52 -0.4 771,516 - CA265_RS03110 0.52 -1.7 771,571 - CA265_RS03110 0.58 -0.1 771,602 + CA265_RS03110 0.62 +0.9 771,622 - CA265_RS03110 0.64 +0.2 771,622 - CA265_RS03110 0.64 -2.1 771,705 + CA265_RS03110 0.73 +2.5 771,781 - CA265_RS03110 0.81 -1.1 771,852 - CA265_RS03110 0.89 -0.2 771,866 - -1.1 771,868 - -0.6 771,925 + +3.0 772,022 + +0.7 772,022 + +0.7 772,022 + +1.2 772,042 + -0.9 772,042 + +0.2 772,204 - CA265_RS03115 0.39 -0.0 772,329 + CA265_RS03115 0.66 +1.1 772,361 + CA265_RS03115 0.73 +0.7 772,454 - -0.2 772,488 - -0.1 772,553 - CA265_RS03120 0.13 +0.4 772,588 - CA265_RS03120 0.19 -0.2 772,610 + CA265_RS03120 0.24 +1.3 772,736 + CA265_RS03120 0.48 +0.2 772,826 - CA265_RS03120 0.65 -1.2 772,869 - CA265_RS03120 0.73 +0.0 772,879 + CA265_RS03120 0.75 -0.2
Or see this region's nucleotide sequence