Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03085 and CA265_RS03090 are separated by 614 nucleotides CA265_RS03090 and CA265_RS03095 are separated by 154 nucleotides CA265_RS03095 and CA265_RS03100 are separated by 158 nucleotides
CA265_RS03085: CA265_RS03085 - hypothetical protein, at 763,236 to 763,688
_RS03085
CA265_RS03090: CA265_RS03090 - DNA polymerase III subunit alpha, at 764,303 to 768,736
_RS03090
CA265_RS03095: CA265_RS03095 - thioredoxin, at 768,891 to 769,211
_RS03095
CA265_RS03100: CA265_RS03100 - glucose dehydrogenase, at 769,370 to 770,581
_RS03100
Position (kb)
764
765
766
767
768
769 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 763.341 kb on - strand, within CA265_RS03085 at 763.385 kb on + strand, within CA265_RS03085 at 763.388 kb on + strand, within CA265_RS03085 at 763.389 kb on - strand, within CA265_RS03085 at 763.389 kb on - strand, within CA265_RS03085 at 763.497 kb on - strand, within CA265_RS03085 at 763.535 kb on + strand, within CA265_RS03085 at 763.536 kb on - strand, within CA265_RS03085 at 763.540 kb on - strand, within CA265_RS03085 at 763.550 kb on - strand, within CA265_RS03085 at 763.569 kb on + strand, within CA265_RS03085 at 763.718 kb on + strand at 763.736 kb on - strand at 763.784 kb on + strand at 763.785 kb on - strand at 763.831 kb on + strand at 763.845 kb on - strand at 763.851 kb on - strand at 763.855 kb on + strand at 763.855 kb on + strand at 763.856 kb on - strand at 763.924 kb on - strand at 763.936 kb on + strand at 763.943 kb on + strand at 763.999 kb on - strand at 764.021 kb on - strand at 764.135 kb on - strand at 764.177 kb on - strand at 764.189 kb on - strand at 768.787 kb on + strand at 768.787 kb on + strand at 768.865 kb on + strand at 769.213 kb on + strand at 769.214 kb on - strand at 769.282 kb on + strand at 769.395 kb on + strand at 769.414 kb on + strand at 769.490 kb on + strand at 769.531 kb on - strand, within CA265_RS03100 at 769.553 kb on - strand, within CA265_RS03100 at 769.555 kb on + strand, within CA265_RS03100 at 769.702 kb on - strand, within CA265_RS03100 at 769.702 kb on - strand, within CA265_RS03100 at 769.728 kb on + strand, within CA265_RS03100 at 769.735 kb on - strand, within CA265_RS03100
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 763,341 - CA265_RS03085 0.23 +1.8 763,385 + CA265_RS03085 0.33 +0.5 763,388 + CA265_RS03085 0.34 -0.4 763,389 - CA265_RS03085 0.34 -1.2 763,389 - CA265_RS03085 0.34 +0.6 763,497 - CA265_RS03085 0.58 +0.2 763,535 + CA265_RS03085 0.66 +0.1 763,536 - CA265_RS03085 0.66 +1.1 763,540 - CA265_RS03085 0.67 +1.3 763,550 - CA265_RS03085 0.69 -0.3 763,569 + CA265_RS03085 0.74 -0.4 763,718 + -1.2 763,736 - -0.6 763,784 + +0.0 763,785 - -1.6 763,831 + -0.8 763,845 - +0.6 763,851 - -1.9 763,855 + +0.5 763,855 + +0.3 763,856 - -1.1 763,924 - +0.2 763,936 + +0.1 763,943 + -1.1 763,999 - -0.1 764,021 - -0.1 764,135 - -0.6 764,177 - -1.2 764,189 - -1.2 768,787 + -0.6 768,787 + +0.4 768,865 + +1.0 769,213 + +2.3 769,214 - +1.8 769,282 + +0.5 769,395 + +0.7 769,414 + +0.6 769,490 + +0.5 769,531 - CA265_RS03100 0.13 -0.1 769,553 - CA265_RS03100 0.15 -0.1 769,555 + CA265_RS03100 0.15 -0.2 769,702 - CA265_RS03100 0.27 -0.4 769,702 - CA265_RS03100 0.27 +0.3 769,728 + CA265_RS03100 0.30 +0.9 769,735 - CA265_RS03100 0.30 -0.3
Or see this region's nucleotide sequence