Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02580 and CA265_RS02585 are separated by 222 nucleotides CA265_RS02585 and CA265_RS02590 are separated by 15 nucleotides
CA265_RS02580: CA265_RS02580 - hypothetical protein, at 646,624 to 647,565
_RS02580
CA265_RS02585: CA265_RS02585 - SusC/RagA family protein, at 647,788 to 651,039
_RS02585
CA265_RS02590: CA265_RS02590 - RagB/SusD family nutrient uptake outer membrane protein, at 651,055 to 652,578
_RS02590
Position (kb)
647
648
649
650
651
652 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 646.805 kb on - strand, within CA265_RS02580 at 646.805 kb on - strand, within CA265_RS02580 at 646.833 kb on + strand, within CA265_RS02580 at 646.833 kb on + strand, within CA265_RS02580 at 646.873 kb on - strand, within CA265_RS02580 at 646.876 kb on - strand, within CA265_RS02580 at 646.886 kb on + strand, within CA265_RS02580 at 646.941 kb on - strand, within CA265_RS02580 at 647.019 kb on + strand, within CA265_RS02580 at 647.116 kb on - strand, within CA265_RS02580 at 647.120 kb on + strand, within CA265_RS02580 at 647.125 kb on - strand, within CA265_RS02580 at 647.138 kb on + strand, within CA265_RS02580 at 647.138 kb on + strand, within CA265_RS02580 at 647.139 kb on - strand, within CA265_RS02580 at 647.142 kb on + strand, within CA265_RS02580 at 647.143 kb on - strand, within CA265_RS02580 at 647.143 kb on - strand, within CA265_RS02580 at 647.210 kb on + strand, within CA265_RS02580 at 647.211 kb on - strand, within CA265_RS02580 at 647.211 kb on - strand, within CA265_RS02580 at 647.211 kb on - strand, within CA265_RS02580 at 647.376 kb on - strand, within CA265_RS02580 at 647.393 kb on - strand, within CA265_RS02580 at 647.497 kb on - strand at 647.557 kb on + strand at 647.557 kb on + strand at 647.558 kb on - strand at 647.568 kb on + strand at 647.662 kb on - strand at 647.686 kb on + strand at 647.710 kb on - strand at 647.728 kb on + strand at 647.729 kb on - strand at 647.730 kb on + strand at 647.730 kb on + strand at 647.730 kb on + strand at 647.730 kb on + strand at 647.731 kb on - strand at 647.731 kb on - strand at 647.754 kb on - strand at 647.842 kb on - strand at 647.915 kb on - strand at 647.915 kb on - strand at 647.915 kb on - strand at 647.952 kb on - strand at 647.952 kb on - strand at 648.038 kb on + strand at 648.039 kb on - strand at 648.039 kb on - strand at 648.042 kb on + strand at 648.042 kb on + strand at 648.043 kb on - strand at 648.043 kb on - strand at 648.043 kb on - strand at 648.055 kb on - strand at 648.155 kb on + strand, within CA265_RS02585 at 648.175 kb on + strand, within CA265_RS02585 at 648.202 kb on - strand, within CA265_RS02585 at 648.242 kb on + strand, within CA265_RS02585 at 648.270 kb on + strand, within CA265_RS02585 at 648.294 kb on + strand, within CA265_RS02585 at 648.294 kb on + strand, within CA265_RS02585 at 648.407 kb on + strand, within CA265_RS02585 at 648.441 kb on - strand, within CA265_RS02585 at 648.446 kb on + strand, within CA265_RS02585 at 648.508 kb on - strand, within CA265_RS02585 at 648.529 kb on + strand, within CA265_RS02585 at 648.533 kb on + strand, within CA265_RS02585 at 648.582 kb on + strand, within CA265_RS02585 at 648.583 kb on - strand, within CA265_RS02585 at 648.602 kb on + strand, within CA265_RS02585 at 648.603 kb on - strand, within CA265_RS02585 at 648.603 kb on - strand, within CA265_RS02585 at 648.741 kb on - strand, within CA265_RS02585 at 648.801 kb on + strand, within CA265_RS02585 at 648.809 kb on + strand, within CA265_RS02585 at 648.904 kb on + strand, within CA265_RS02585 at 648.915 kb on + strand, within CA265_RS02585 at 648.951 kb on - strand, within CA265_RS02585 at 649.081 kb on - strand, within CA265_RS02585 at 649.114 kb on + strand, within CA265_RS02585 at 649.142 kb on - strand, within CA265_RS02585 at 649.145 kb on - strand, within CA265_RS02585 at 649.165 kb on + strand, within CA265_RS02585 at 649.425 kb on + strand, within CA265_RS02585 at 649.478 kb on + strand, within CA265_RS02585 at 649.478 kb on + strand, within CA265_RS02585 at 649.485 kb on + strand, within CA265_RS02585 at 649.559 kb on + strand, within CA265_RS02585 at 649.600 kb on + strand, within CA265_RS02585 at 649.602 kb on + strand, within CA265_RS02585 at 649.602 kb on + strand, within CA265_RS02585 at 649.603 kb on - strand, within CA265_RS02585 at 649.690 kb on + strand, within CA265_RS02585 at 649.692 kb on + strand, within CA265_RS02585 at 649.696 kb on + strand, within CA265_RS02585 at 649.761 kb on + strand, within CA265_RS02585 at 649.922 kb on + strand, within CA265_RS02585 at 649.923 kb on - strand, within CA265_RS02585 at 649.973 kb on - strand, within CA265_RS02585 at 649.976 kb on + strand, within CA265_RS02585 at 649.977 kb on - strand, within CA265_RS02585 at 650.045 kb on + strand, within CA265_RS02585 at 650.064 kb on - strand, within CA265_RS02585 at 650.064 kb on - strand, within CA265_RS02585 at 650.080 kb on + strand, within CA265_RS02585 at 650.081 kb on - strand, within CA265_RS02585 at 650.209 kb on - strand, within CA265_RS02585 at 650.229 kb on + strand, within CA265_RS02585 at 650.229 kb on + strand, within CA265_RS02585 at 650.230 kb on - strand, within CA265_RS02585 at 650.230 kb on - strand, within CA265_RS02585 at 650.309 kb on + strand, within CA265_RS02585 at 650.310 kb on - strand, within CA265_RS02585 at 650.366 kb on + strand, within CA265_RS02585 at 650.369 kb on - strand, within CA265_RS02585 at 650.369 kb on - strand, within CA265_RS02585 at 650.375 kb on - strand, within CA265_RS02585 at 650.378 kb on - strand, within CA265_RS02585 at 650.378 kb on - strand, within CA265_RS02585 at 650.378 kb on - strand, within CA265_RS02585 at 650.424 kb on + strand, within CA265_RS02585 at 650.424 kb on + strand, within CA265_RS02585 at 650.431 kb on - strand, within CA265_RS02585 at 650.512 kb on - strand, within CA265_RS02585 at 650.666 kb on - strand, within CA265_RS02585 at 650.666 kb on - strand, within CA265_RS02585 at 650.666 kb on - strand, within CA265_RS02585 at 650.830 kb on - strand at 650.834 kb on + strand at 650.834 kb on + strand at 650.835 kb on - strand at 650.913 kb on + strand at 651.013 kb on - strand at 651.023 kb on + strand at 651.029 kb on + strand at 651.035 kb on + strand at 651.073 kb on + strand at 651.076 kb on - strand at 651.126 kb on + strand at 651.126 kb on + strand at 651.183 kb on + strand at 651.274 kb on - strand, within CA265_RS02590 at 651.274 kb on - strand, within CA265_RS02590 at 651.274 kb on - strand, within CA265_RS02590 at 651.322 kb on - strand, within CA265_RS02590 at 651.328 kb on - strand, within CA265_RS02590 at 651.328 kb on - strand, within CA265_RS02590 at 651.381 kb on - strand, within CA265_RS02590 at 651.466 kb on + strand, within CA265_RS02590 at 651.466 kb on + strand, within CA265_RS02590 at 651.466 kb on + strand, within CA265_RS02590 at 651.541 kb on - strand, within CA265_RS02590 at 651.623 kb on + strand, within CA265_RS02590 at 651.720 kb on - strand, within CA265_RS02590 at 651.720 kb on - strand, within CA265_RS02590 at 651.720 kb on - strand, within CA265_RS02590 at 651.721 kb on + strand, within CA265_RS02590 at 651.775 kb on - strand, within CA265_RS02590 at 651.818 kb on + strand, within CA265_RS02590 at 651.818 kb on + strand, within CA265_RS02590 at 651.819 kb on - strand, within CA265_RS02590 at 651.852 kb on + strand, within CA265_RS02590 at 651.852 kb on + strand, within CA265_RS02590 at 651.888 kb on + strand, within CA265_RS02590 at 651.888 kb on + strand, within CA265_RS02590 at 651.889 kb on - strand, within CA265_RS02590 at 651.918 kb on - strand, within CA265_RS02590 at 651.930 kb on + strand, within CA265_RS02590 at 651.947 kb on - strand, within CA265_RS02590 at 651.964 kb on + strand, within CA265_RS02590 at 651.986 kb on - strand, within CA265_RS02590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 646,805 - CA265_RS02580 0.19 +0.8 646,805 - CA265_RS02580 0.19 +0.3 646,833 + CA265_RS02580 0.22 +2.1 646,833 + CA265_RS02580 0.22 +1.3 646,873 - CA265_RS02580 0.26 -0.1 646,876 - CA265_RS02580 0.27 -0.5 646,886 + CA265_RS02580 0.28 +1.2 646,941 - CA265_RS02580 0.34 +0.6 647,019 + CA265_RS02580 0.42 +2.1 647,116 - CA265_RS02580 0.52 -0.9 647,120 + CA265_RS02580 0.53 +2.2 647,125 - CA265_RS02580 0.53 -0.2 647,138 + CA265_RS02580 0.55 +2.0 647,138 + CA265_RS02580 0.55 +1.7 647,139 - CA265_RS02580 0.55 -1.9 647,142 + CA265_RS02580 0.55 +1.9 647,143 - CA265_RS02580 0.55 -0.4 647,143 - CA265_RS02580 0.55 -0.0 647,210 + CA265_RS02580 0.62 +1.5 647,211 - CA265_RS02580 0.62 +0.0 647,211 - CA265_RS02580 0.62 -0.0 647,211 - CA265_RS02580 0.62 +0.6 647,376 - CA265_RS02580 0.80 +0.6 647,393 - CA265_RS02580 0.82 +0.4 647,497 - +0.7 647,557 + +0.6 647,557 + +2.0 647,558 - -0.4 647,568 + +1.5 647,662 - +2.0 647,686 + -1.3 647,710 - +0.8 647,728 + +0.8 647,729 - -0.8 647,730 + -2.3 647,730 + +0.3 647,730 + +0.1 647,730 + +0.6 647,731 - +0.3 647,731 - +1.0 647,754 - -0.1 647,842 - +0.4 647,915 - +0.3 647,915 - -0.2 647,915 - +1.7 647,952 - -0.4 647,952 - -0.2 648,038 + +0.4 648,039 - +0.6 648,039 - -1.7 648,042 + +0.3 648,042 + -0.5 648,043 - +0.4 648,043 - -0.1 648,043 - -1.0 648,055 - -1.7 648,155 + CA265_RS02585 0.11 +0.4 648,175 + CA265_RS02585 0.12 -0.4 648,202 - CA265_RS02585 0.13 -1.0 648,242 + CA265_RS02585 0.14 -0.1 648,270 + CA265_RS02585 0.15 +1.7 648,294 + CA265_RS02585 0.16 +1.0 648,294 + CA265_RS02585 0.16 +0.6 648,407 + CA265_RS02585 0.19 +1.0 648,441 - CA265_RS02585 0.20 +0.5 648,446 + CA265_RS02585 0.20 +0.4 648,508 - CA265_RS02585 0.22 -0.5 648,529 + CA265_RS02585 0.23 +0.5 648,533 + CA265_RS02585 0.23 +1.4 648,582 + CA265_RS02585 0.24 +0.6 648,583 - CA265_RS02585 0.24 +0.1 648,602 + CA265_RS02585 0.25 -0.4 648,603 - CA265_RS02585 0.25 +1.0 648,603 - CA265_RS02585 0.25 -0.1 648,741 - CA265_RS02585 0.29 -0.7 648,801 + CA265_RS02585 0.31 -1.2 648,809 + CA265_RS02585 0.31 -1.5 648,904 + CA265_RS02585 0.34 +0.6 648,915 + CA265_RS02585 0.35 +0.3 648,951 - CA265_RS02585 0.36 +0.6 649,081 - CA265_RS02585 0.40 +0.3 649,114 + CA265_RS02585 0.41 +0.1 649,142 - CA265_RS02585 0.42 +0.4 649,145 - CA265_RS02585 0.42 +0.3 649,165 + CA265_RS02585 0.42 -0.7 649,425 + CA265_RS02585 0.50 +1.1 649,478 + CA265_RS02585 0.52 +0.3 649,478 + CA265_RS02585 0.52 +0.6 649,485 + CA265_RS02585 0.52 -0.3 649,559 + CA265_RS02585 0.54 +0.2 649,600 + CA265_RS02585 0.56 +1.0 649,602 + CA265_RS02585 0.56 -0.3 649,602 + CA265_RS02585 0.56 +0.2 649,603 - CA265_RS02585 0.56 -0.2 649,690 + CA265_RS02585 0.58 +0.4 649,692 + CA265_RS02585 0.59 -1.4 649,696 + CA265_RS02585 0.59 +0.4 649,761 + CA265_RS02585 0.61 +1.2 649,922 + CA265_RS02585 0.66 -0.5 649,923 - CA265_RS02585 0.66 +1.4 649,973 - CA265_RS02585 0.67 +1.1 649,976 + CA265_RS02585 0.67 -0.8 649,977 - CA265_RS02585 0.67 +0.0 650,045 + CA265_RS02585 0.69 -0.2 650,064 - CA265_RS02585 0.70 +0.1 650,064 - CA265_RS02585 0.70 -0.2 650,080 + CA265_RS02585 0.70 +0.5 650,081 - CA265_RS02585 0.71 +1.7 650,209 - CA265_RS02585 0.74 +0.9 650,229 + CA265_RS02585 0.75 +0.2 650,229 + CA265_RS02585 0.75 -1.4 650,230 - CA265_RS02585 0.75 -0.3 650,230 - CA265_RS02585 0.75 +0.5 650,309 + CA265_RS02585 0.78 -1.3 650,310 - CA265_RS02585 0.78 +1.3 650,366 + CA265_RS02585 0.79 -0.9 650,369 - CA265_RS02585 0.79 +0.4 650,369 - CA265_RS02585 0.79 +0.8 650,375 - CA265_RS02585 0.80 +0.9 650,378 - CA265_RS02585 0.80 -0.1 650,378 - CA265_RS02585 0.80 +0.7 650,378 - CA265_RS02585 0.80 +0.6 650,424 + CA265_RS02585 0.81 +1.3 650,424 + CA265_RS02585 0.81 -0.8 650,431 - CA265_RS02585 0.81 -0.4 650,512 - CA265_RS02585 0.84 +0.3 650,666 - CA265_RS02585 0.88 -0.4 650,666 - CA265_RS02585 0.88 +1.0 650,666 - CA265_RS02585 0.88 -0.7 650,830 - +0.3 650,834 + -0.3 650,834 + +0.2 650,835 - -0.1 650,913 + -0.4 651,013 - -2.4 651,023 + +0.6 651,029 + -0.0 651,035 + +0.1 651,073 + +0.1 651,076 - +0.3 651,126 + +1.2 651,126 + +0.9 651,183 + -0.2 651,274 - CA265_RS02590 0.14 -0.5 651,274 - CA265_RS02590 0.14 -0.4 651,274 - CA265_RS02590 0.14 +0.9 651,322 - CA265_RS02590 0.18 -0.3 651,328 - CA265_RS02590 0.18 -0.3 651,328 - CA265_RS02590 0.18 +0.2 651,381 - CA265_RS02590 0.21 -0.3 651,466 + CA265_RS02590 0.27 +0.1 651,466 + CA265_RS02590 0.27 +1.6 651,466 + CA265_RS02590 0.27 +0.7 651,541 - CA265_RS02590 0.32 -1.0 651,623 + CA265_RS02590 0.37 +0.5 651,720 - CA265_RS02590 0.44 -3.0 651,720 - CA265_RS02590 0.44 +0.5 651,720 - CA265_RS02590 0.44 -0.3 651,721 + CA265_RS02590 0.44 -0.1 651,775 - CA265_RS02590 0.47 -1.6 651,818 + CA265_RS02590 0.50 +0.8 651,818 + CA265_RS02590 0.50 +0.7 651,819 - CA265_RS02590 0.50 +0.1 651,852 + CA265_RS02590 0.52 +0.8 651,852 + CA265_RS02590 0.52 +1.1 651,888 + CA265_RS02590 0.55 +0.0 651,888 + CA265_RS02590 0.55 +0.6 651,889 - CA265_RS02590 0.55 -0.3 651,918 - CA265_RS02590 0.57 +0.1 651,930 + CA265_RS02590 0.57 -0.0 651,947 - CA265_RS02590 0.59 -0.0 651,964 + CA265_RS02590 0.60 +0.2 651,986 - CA265_RS02590 0.61 -0.7
Or see this region's nucleotide sequence