Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02295 and CA265_RS02300 are separated by 6 nucleotides CA265_RS02300 and CA265_RS02305 are separated by 92 nucleotides CA265_RS02305 and CA265_RS02310 are separated by 359 nucleotides CA265_RS02310 and CA265_RS02315 are separated by 239 nucleotides
CA265_RS02295: CA265_RS02295 - metal-binding protein, at 573,706 to 573,954
_RS02295
CA265_RS02300: CA265_RS02300 - cysteine methyltransferase, at 573,961 to 574,800
_RS02300
CA265_RS02305: CA265_RS02305 - hypothetical protein, at 574,893 to 575,429
_RS02305
CA265_RS02310: CA265_RS02310 - hypothetical protein, at 575,789 to 576,154
_RS02310
CA265_RS02315: CA265_RS02315 - single-stranded DNA-binding protein, at 576,394 to 576,741
_RS02315
Position (kb) 574
575
576 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 574.051 kb on + strand, within CA265_RS02300 at 574.052 kb on - strand, within CA265_RS02300 at 574.052 kb on - strand, within CA265_RS02300 at 574.108 kb on + strand, within CA265_RS02300 at 574.126 kb on - strand, within CA265_RS02300 at 574.131 kb on + strand, within CA265_RS02300 at 574.157 kb on + strand, within CA265_RS02300 at 574.181 kb on - strand, within CA265_RS02300 at 574.181 kb on - strand, within CA265_RS02300 at 574.228 kb on + strand, within CA265_RS02300 at 574.240 kb on - strand, within CA265_RS02300 at 574.277 kb on + strand, within CA265_RS02300 at 574.278 kb on - strand, within CA265_RS02300 at 574.299 kb on + strand, within CA265_RS02300 at 574.306 kb on + strand, within CA265_RS02300 at 574.307 kb on - strand, within CA265_RS02300 at 574.605 kb on - strand, within CA265_RS02300 at 574.614 kb on + strand, within CA265_RS02300 at 574.619 kb on + strand, within CA265_RS02300 at 574.619 kb on + strand, within CA265_RS02300 at 574.641 kb on + strand, within CA265_RS02300 at 574.687 kb on + strand, within CA265_RS02300 at 574.714 kb on + strand, within CA265_RS02300 at 574.811 kb on - strand at 574.816 kb on - strand at 574.898 kb on + strand at 574.970 kb on - strand, within CA265_RS02305 at 575.052 kb on - strand, within CA265_RS02305 at 575.059 kb on - strand, within CA265_RS02305 at 575.081 kb on + strand, within CA265_RS02305 at 575.081 kb on + strand, within CA265_RS02305 at 575.089 kb on - strand, within CA265_RS02305 at 575.111 kb on + strand, within CA265_RS02305 at 575.146 kb on - strand, within CA265_RS02305 at 575.190 kb on - strand, within CA265_RS02305 at 575.190 kb on - strand, within CA265_RS02305 at 575.305 kb on + strand, within CA265_RS02305 at 575.306 kb on - strand, within CA265_RS02305 at 575.332 kb on - strand, within CA265_RS02305 at 575.362 kb on + strand, within CA265_RS02305 at 575.367 kb on + strand, within CA265_RS02305 at 575.368 kb on - strand, within CA265_RS02305 at 575.440 kb on - strand at 575.503 kb on + strand at 575.504 kb on - strand at 575.582 kb on - strand at 575.582 kb on - strand at 575.655 kb on + strand at 575.656 kb on - strand at 575.722 kb on - strand at 575.770 kb on + strand at 575.771 kb on - strand at 575.829 kb on + strand, within CA265_RS02310 at 575.829 kb on + strand, within CA265_RS02310 at 575.830 kb on - strand, within CA265_RS02310 at 575.830 kb on - strand, within CA265_RS02310 at 575.830 kb on - strand, within CA265_RS02310 at 575.860 kb on - strand, within CA265_RS02310 at 575.877 kb on + strand, within CA265_RS02310 at 575.905 kb on + strand, within CA265_RS02310 at 575.906 kb on - strand, within CA265_RS02310 at 575.907 kb on + strand, within CA265_RS02310 at 575.907 kb on + strand, within CA265_RS02310 at 575.907 kb on + strand, within CA265_RS02310 at 575.908 kb on - strand, within CA265_RS02310 at 575.908 kb on - strand, within CA265_RS02310 at 575.911 kb on + strand, within CA265_RS02310 at 576.015 kb on - strand, within CA265_RS02310 at 576.020 kb on - strand, within CA265_RS02310 at 576.144 kb on - strand at 576.144 kb on - strand at 576.158 kb on - strand at 576.244 kb on + strand at 576.285 kb on + strand at 576.285 kb on + strand at 576.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 574,051 + CA265_RS02300 0.11 -0.3 574,052 - CA265_RS02300 0.11 +0.9 574,052 - CA265_RS02300 0.11 -1.2 574,108 + CA265_RS02300 0.17 -0.2 574,126 - CA265_RS02300 0.20 -0.8 574,131 + CA265_RS02300 0.20 +0.7 574,157 + CA265_RS02300 0.23 -1.1 574,181 - CA265_RS02300 0.26 +0.5 574,181 - CA265_RS02300 0.26 +0.1 574,228 + CA265_RS02300 0.32 +0.2 574,240 - CA265_RS02300 0.33 +0.2 574,277 + CA265_RS02300 0.38 +0.9 574,278 - CA265_RS02300 0.38 -2.0 574,299 + CA265_RS02300 0.40 -0.8 574,306 + CA265_RS02300 0.41 +1.4 574,307 - CA265_RS02300 0.41 +0.6 574,605 - CA265_RS02300 0.77 -0.3 574,614 + CA265_RS02300 0.78 -1.5 574,619 + CA265_RS02300 0.78 +1.7 574,619 + CA265_RS02300 0.78 -0.4 574,641 + CA265_RS02300 0.81 +0.5 574,687 + CA265_RS02300 0.86 -1.1 574,714 + CA265_RS02300 0.90 -0.3 574,811 - -0.5 574,816 - +0.0 574,898 + +1.4 574,970 - CA265_RS02305 0.14 +0.6 575,052 - CA265_RS02305 0.30 +1.1 575,059 - CA265_RS02305 0.31 +0.1 575,081 + CA265_RS02305 0.35 -2.0 575,081 + CA265_RS02305 0.35 +0.4 575,089 - CA265_RS02305 0.36 +0.3 575,111 + CA265_RS02305 0.41 +0.6 575,146 - CA265_RS02305 0.47 +0.4 575,190 - CA265_RS02305 0.55 -0.6 575,190 - CA265_RS02305 0.55 +1.0 575,305 + CA265_RS02305 0.77 -0.6 575,306 - CA265_RS02305 0.77 +2.0 575,332 - CA265_RS02305 0.82 +1.0 575,362 + CA265_RS02305 0.87 +0.3 575,367 + CA265_RS02305 0.88 +1.1 575,368 - CA265_RS02305 0.88 -0.7 575,440 - -1.4 575,503 + -0.5 575,504 - -0.0 575,582 - +0.7 575,582 - +0.2 575,655 + -0.3 575,656 - -0.6 575,722 - -0.1 575,770 + -0.1 575,771 - -0.2 575,829 + CA265_RS02310 0.11 +0.5 575,829 + CA265_RS02310 0.11 -0.3 575,830 - CA265_RS02310 0.11 +0.6 575,830 - CA265_RS02310 0.11 +0.3 575,830 - CA265_RS02310 0.11 -0.5 575,860 - CA265_RS02310 0.19 -1.3 575,877 + CA265_RS02310 0.24 +0.2 575,905 + CA265_RS02310 0.32 +1.4 575,906 - CA265_RS02310 0.32 -0.7 575,907 + CA265_RS02310 0.32 +1.4 575,907 + CA265_RS02310 0.32 +0.6 575,907 + CA265_RS02310 0.32 +0.7 575,908 - CA265_RS02310 0.33 +0.7 575,908 - CA265_RS02310 0.33 -1.2 575,911 + CA265_RS02310 0.33 +0.5 576,015 - CA265_RS02310 0.62 +1.0 576,020 - CA265_RS02310 0.63 -1.0 576,144 - -0.1 576,144 - +0.2 576,158 - -3.2 576,244 + +0.9 576,285 + -0.3 576,285 + -3.0 576,420 - +0.0
Or see this region's nucleotide sequence