Experiment: R2A with Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00920 and CA265_RS00925 are separated by 150 nucleotides CA265_RS00925 and CA265_RS00930 are separated by 88 nucleotides
CA265_RS00920: CA265_RS00920 - hypothetical protein, at 227,711 to 228,439
_RS00920
CA265_RS00925: CA265_RS00925 - hypothetical protein, at 228,590 to 229,051
_RS00925
CA265_RS00930: CA265_RS00930 - CusA/CzcA family heavy metal efflux RND transporter, at 229,140 to 233,504
_RS00930
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 227.613 kb on + strand at 227.648 kb on + strand at 227.649 kb on - strand at 227.807 kb on - strand, within CA265_RS00920 at 227.853 kb on + strand, within CA265_RS00920 at 227.949 kb on + strand, within CA265_RS00920 at 227.949 kb on + strand, within CA265_RS00920 at 227.956 kb on + strand, within CA265_RS00920 at 227.966 kb on - strand, within CA265_RS00920 at 228.068 kb on + strand, within CA265_RS00920 at 228.069 kb on - strand, within CA265_RS00920 at 228.069 kb on - strand, within CA265_RS00920 at 228.091 kb on - strand, within CA265_RS00920 at 228.138 kb on - strand, within CA265_RS00920 at 228.196 kb on + strand, within CA265_RS00920 at 228.255 kb on + strand, within CA265_RS00920 at 228.272 kb on - strand, within CA265_RS00920 at 228.295 kb on + strand, within CA265_RS00920 at 228.296 kb on - strand, within CA265_RS00920 at 228.301 kb on - strand, within CA265_RS00920 at 228.313 kb on - strand, within CA265_RS00920 at 228.357 kb on - strand, within CA265_RS00920 at 228.370 kb on + strand at 228.380 kb on - strand at 228.430 kb on - strand at 228.432 kb on - strand at 228.451 kb on + strand at 228.455 kb on + strand at 228.493 kb on + strand at 228.551 kb on + strand at 228.578 kb on - strand at 228.659 kb on + strand, within CA265_RS00925 at 228.662 kb on - strand, within CA265_RS00925 at 228.683 kb on - strand, within CA265_RS00925 at 228.736 kb on + strand, within CA265_RS00925 at 228.754 kb on + strand, within CA265_RS00925 at 228.754 kb on + strand, within CA265_RS00925 at 228.824 kb on + strand, within CA265_RS00925 at 228.901 kb on + strand, within CA265_RS00925 at 228.901 kb on + strand, within CA265_RS00925 at 228.902 kb on - strand, within CA265_RS00925 at 228.967 kb on - strand, within CA265_RS00925 at 229.114 kb on - strand at 229.114 kb on - strand at 229.148 kb on + strand at 229.148 kb on + strand at 229.224 kb on + strand at 229.240 kb on + strand at 229.240 kb on + strand at 229.319 kb on + strand at 229.331 kb on - strand at 229.359 kb on - strand at 229.449 kb on + strand at 229.449 kb on + strand at 229.450 kb on - strand at 229.561 kb on - strand at 229.567 kb on - strand at 229.590 kb on + strand, within CA265_RS00930 at 229.591 kb on - strand, within CA265_RS00930 at 229.591 kb on - strand, within CA265_RS00930 at 229.598 kb on + strand, within CA265_RS00930 at 229.599 kb on - strand, within CA265_RS00930 at 229.599 kb on - strand, within CA265_RS00930 at 229.603 kb on - strand, within CA265_RS00930 at 229.603 kb on - strand, within CA265_RS00930 at 229.603 kb on - strand, within CA265_RS00930 at 229.603 kb on - strand, within CA265_RS00930 at 229.622 kb on + strand, within CA265_RS00930 at 229.642 kb on + strand, within CA265_RS00930 at 229.757 kb on + strand, within CA265_RS00930 at 229.812 kb on + strand, within CA265_RS00930 at 229.839 kb on + strand, within CA265_RS00930 at 229.945 kb on + strand, within CA265_RS00930 at 229.946 kb on - strand, within CA265_RS00930 at 229.986 kb on - strand, within CA265_RS00930 at 230.050 kb on + strand, within CA265_RS00930
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Sisomicin 0.008 mg/ml remove 227,613 + +0.1 227,648 + +0.1 227,649 - -0.5 227,807 - CA265_RS00920 0.13 +0.9 227,853 + CA265_RS00920 0.19 +2.1 227,949 + CA265_RS00920 0.33 +0.5 227,949 + CA265_RS00920 0.33 -2.0 227,956 + CA265_RS00920 0.34 -0.3 227,966 - CA265_RS00920 0.35 -0.3 228,068 + CA265_RS00920 0.49 +1.3 228,069 - CA265_RS00920 0.49 +1.0 228,069 - CA265_RS00920 0.49 +0.4 228,091 - CA265_RS00920 0.52 -0.6 228,138 - CA265_RS00920 0.59 -0.7 228,196 + CA265_RS00920 0.67 +1.7 228,255 + CA265_RS00920 0.75 +0.6 228,272 - CA265_RS00920 0.77 +0.0 228,295 + CA265_RS00920 0.80 +0.8 228,296 - CA265_RS00920 0.80 -0.0 228,301 - CA265_RS00920 0.81 +0.0 228,313 - CA265_RS00920 0.83 -0.0 228,357 - CA265_RS00920 0.89 +0.8 228,370 + +0.5 228,380 - -0.4 228,430 - -1.5 228,432 - -0.7 228,451 + +1.3 228,455 + +1.0 228,493 + +0.2 228,551 + -0.8 228,578 - +0.2 228,659 + CA265_RS00925 0.15 -1.3 228,662 - CA265_RS00925 0.16 +0.3 228,683 - CA265_RS00925 0.20 +0.7 228,736 + CA265_RS00925 0.32 +0.5 228,754 + CA265_RS00925 0.35 -2.8 228,754 + CA265_RS00925 0.35 -2.0 228,824 + CA265_RS00925 0.51 -2.2 228,901 + CA265_RS00925 0.67 -1.7 228,901 + CA265_RS00925 0.67 -0.5 228,902 - CA265_RS00925 0.68 -0.5 228,967 - CA265_RS00925 0.82 -0.6 229,114 - -0.3 229,114 - -0.1 229,148 + +0.2 229,148 + -2.3 229,224 + +0.4 229,240 + -0.0 229,240 + -1.5 229,319 + +0.0 229,331 - -0.3 229,359 - +0.3 229,449 + -1.1 229,449 + +0.9 229,450 - -0.6 229,561 - -0.0 229,567 - -1.1 229,590 + CA265_RS00930 0.10 -1.7 229,591 - CA265_RS00930 0.10 -0.7 229,591 - CA265_RS00930 0.10 +0.1 229,598 + CA265_RS00930 0.10 -0.3 229,599 - CA265_RS00930 0.11 -0.3 229,599 - CA265_RS00930 0.11 +1.8 229,603 - CA265_RS00930 0.11 -1.4 229,603 - CA265_RS00930 0.11 +0.5 229,603 - CA265_RS00930 0.11 -0.1 229,603 - CA265_RS00930 0.11 -0.7 229,622 + CA265_RS00930 0.11 -1.7 229,642 + CA265_RS00930 0.12 +1.4 229,757 + CA265_RS00930 0.14 -0.6 229,812 + CA265_RS00930 0.15 -2.3 229,839 + CA265_RS00930 0.16 -0.2 229,945 + CA265_RS00930 0.18 +0.0 229,946 - CA265_RS00930 0.18 -2.0 229,986 - CA265_RS00930 0.19 -0.3 230,050 + CA265_RS00930 0.21 -0.2
Or see this region's nucleotide sequence