Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_71 and Ac3H11_72 are separated by 166 nucleotides Ac3H11_72 and Ac3H11_73 are separated by 215 nucleotides Ac3H11_73 and Ac3H11_74 are separated by 27 nucleotides
Ac3H11_71 - Extracellular ligand-binding receptor, at 75,001 to 76,149
_71
Ac3H11_72 - D-(-)-3-hydroxybutyrate oligomer hydrolase, at 76,316 to 77,191
_72
Ac3H11_73 - Phenylalanyl-tRNA synthetase beta subunit, at 77,407 to 77,874
_73
Ac3H11_74 - hypothetical protein, at 77,902 to 78,144
_74
Position (kb) 76
77
78 Strain fitness (log2 ratio) -2
-1
0
1 at 75.394 kb on + strand, within Ac3H11_71 at 75.394 kb on + strand, within Ac3H11_71 at 75.394 kb on + strand, within Ac3H11_71 at 75.395 kb on - strand, within Ac3H11_71 at 75.395 kb on - strand, within Ac3H11_71 at 75.526 kb on + strand, within Ac3H11_71 at 75.527 kb on - strand, within Ac3H11_71 at 75.527 kb on - strand, within Ac3H11_71 at 75.760 kb on + strand, within Ac3H11_71 at 75.760 kb on + strand, within Ac3H11_71 at 75.761 kb on - strand, within Ac3H11_71 at 75.761 kb on - strand, within Ac3H11_71 at 75.931 kb on + strand, within Ac3H11_71 at 75.932 kb on - strand, within Ac3H11_71 at 76.045 kb on + strand at 76.046 kb on - strand at 76.046 kb on - strand at 76.073 kb on - strand at 76.073 kb on - strand at 76.103 kb on - strand at 76.205 kb on - strand at 76.361 kb on + strand at 76.373 kb on + strand at 76.373 kb on + strand at 76.547 kb on + strand, within Ac3H11_72 at 76.764 kb on - strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.826 kb on + strand, within Ac3H11_72 at 76.965 kb on - strand, within Ac3H11_72 at 76.983 kb on - strand, within Ac3H11_72 at 77.241 kb on - strand at 77.241 kb on - strand at 77.241 kb on - strand at 77.241 kb on - strand at 77.267 kb on + strand at 77.268 kb on - strand at 77.367 kb on + strand at 77.473 kb on + strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.474 kb on - strand, within Ac3H11_73 at 77.668 kb on + strand, within Ac3H11_73 at 77.668 kb on + strand, within Ac3H11_73 at 77.678 kb on - strand, within Ac3H11_73 at 77.866 kb on + strand at 77.866 kb on + strand at 77.867 kb on - strand at 77.867 kb on - strand at 77.872 kb on + strand at 77.873 kb on - strand at 78.012 kb on - strand, within Ac3H11_74 at 78.012 kb on - strand, within Ac3H11_74 at 78.123 kb on - strand at 78.155 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 75,394 + Ac3H11_71 0.34 +0.8 75,394 + Ac3H11_71 0.34 +0.6 75,394 + Ac3H11_71 0.34 +0.2 75,395 - Ac3H11_71 0.34 -1.8 75,395 - Ac3H11_71 0.34 -0.5 75,526 + Ac3H11_71 0.46 -0.4 75,527 - Ac3H11_71 0.46 +1.0 75,527 - Ac3H11_71 0.46 -1.9 75,760 + Ac3H11_71 0.66 -0.9 75,760 + Ac3H11_71 0.66 -0.6 75,761 - Ac3H11_71 0.66 +0.5 75,761 - Ac3H11_71 0.66 -0.6 75,931 + Ac3H11_71 0.81 -0.6 75,932 - Ac3H11_71 0.81 +0.1 76,045 + -1.9 76,046 - +0.6 76,046 - -1.0 76,073 - -0.1 76,073 - +0.2 76,103 - +0.1 76,205 - -1.2 76,361 + -0.5 76,373 + +0.2 76,373 + -1.3 76,547 + Ac3H11_72 0.26 -2.3 76,764 - Ac3H11_72 0.51 -0.7 76,826 + Ac3H11_72 0.58 -0.1 76,826 + Ac3H11_72 0.58 -0.0 76,826 + Ac3H11_72 0.58 -0.7 76,826 + Ac3H11_72 0.58 -0.2 76,965 - Ac3H11_72 0.74 -1.1 76,983 - Ac3H11_72 0.76 +0.7 77,241 - -1.0 77,241 - +0.1 77,241 - +0.7 77,241 - +0.4 77,267 + -1.0 77,268 - -1.4 77,367 + +0.2 77,473 + Ac3H11_73 0.14 -0.1 77,474 - Ac3H11_73 0.14 +0.8 77,474 - Ac3H11_73 0.14 -0.3 77,474 - Ac3H11_73 0.14 -2.0 77,474 - Ac3H11_73 0.14 +0.1 77,668 + Ac3H11_73 0.56 +0.3 77,668 + Ac3H11_73 0.56 -1.4 77,678 - Ac3H11_73 0.58 -1.4 77,866 + +0.2 77,866 + -2.1 77,867 - -0.5 77,867 - +0.1 77,872 + -1.6 77,873 - -0.7 78,012 - Ac3H11_74 0.45 -1.1 78,012 - Ac3H11_74 0.45 -0.5 78,123 - -0.2 78,155 + -0.0
Or see this region's nucleotide sequence