Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4985 and Ac3H11_4986 are separated by 17 nucleotides Ac3H11_4986 and Ac3H11_4987 are separated by 13 nucleotides Ac3H11_4987 and Ac3H11_4988 overlap by 4 nucleotides
Ac3H11_4985 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 148,867 to 149,904
_4985
Ac3H11_4986 - High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1), at 149,922 to 151,103
_4986
Ac3H11_4987 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 151,117 to 151,935
_4987
Ac3H11_4988 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 151,932 to 153,734
_4988
Position (kb)
149
150
151
152 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 149.003 kb on - strand, within Ac3H11_4985 at 149.116 kb on + strand, within Ac3H11_4985 at 149.117 kb on - strand, within Ac3H11_4985 at 149.117 kb on - strand, within Ac3H11_4985 at 149.117 kb on - strand, within Ac3H11_4985 at 149.242 kb on + strand, within Ac3H11_4985 at 149.243 kb on - strand, within Ac3H11_4985 at 149.243 kb on - strand, within Ac3H11_4985 at 149.243 kb on - strand, within Ac3H11_4985 at 149.383 kb on + strand, within Ac3H11_4985 at 149.506 kb on - strand, within Ac3H11_4985 at 149.507 kb on + strand, within Ac3H11_4985 at 149.507 kb on + strand, within Ac3H11_4985 at 149.507 kb on + strand, within Ac3H11_4985 at 149.508 kb on - strand, within Ac3H11_4985 at 149.521 kb on + strand, within Ac3H11_4985 at 149.521 kb on + strand, within Ac3H11_4985 at 149.555 kb on - strand, within Ac3H11_4985 at 149.555 kb on - strand, within Ac3H11_4985 at 149.555 kb on - strand, within Ac3H11_4985 at 149.584 kb on + strand, within Ac3H11_4985 at 149.585 kb on - strand, within Ac3H11_4985 at 149.798 kb on + strand, within Ac3H11_4985 at 149.798 kb on + strand, within Ac3H11_4985 at 149.799 kb on - strand, within Ac3H11_4985 at 149.799 kb on - strand, within Ac3H11_4985 at 149.799 kb on - strand, within Ac3H11_4985 at 149.902 kb on + strand at 150.159 kb on + strand, within Ac3H11_4986 at 150.159 kb on + strand, within Ac3H11_4986 at 150.159 kb on + strand, within Ac3H11_4986 at 150.159 kb on + strand, within Ac3H11_4986 at 150.159 kb on + strand, within Ac3H11_4986 at 150.159 kb on + strand, within Ac3H11_4986 at 150.159 kb on + strand, within Ac3H11_4986 at 150.160 kb on - strand, within Ac3H11_4986 at 150.160 kb on - strand, within Ac3H11_4986 at 150.160 kb on - strand, within Ac3H11_4986 at 150.160 kb on - strand, within Ac3H11_4986 at 150.181 kb on - strand, within Ac3H11_4986 at 150.181 kb on - strand, within Ac3H11_4986 at 150.181 kb on - strand, within Ac3H11_4986 at 150.244 kb on - strand, within Ac3H11_4986 at 150.247 kb on - strand, within Ac3H11_4986 at 150.357 kb on + strand, within Ac3H11_4986 at 150.358 kb on - strand, within Ac3H11_4986 at 150.460 kb on - strand, within Ac3H11_4986 at 150.612 kb on + strand, within Ac3H11_4986 at 150.613 kb on - strand, within Ac3H11_4986 at 150.630 kb on + strand, within Ac3H11_4986 at 150.631 kb on - strand, within Ac3H11_4986 at 150.631 kb on - strand, within Ac3H11_4986 at 150.631 kb on - strand, within Ac3H11_4986 at 150.631 kb on - strand, within Ac3H11_4986 at 150.631 kb on - strand, within Ac3H11_4986 at 150.631 kb on - strand, within Ac3H11_4986 at 150.783 kb on + strand, within Ac3H11_4986 at 150.784 kb on - strand, within Ac3H11_4986 at 150.784 kb on - strand, within Ac3H11_4986 at 150.786 kb on + strand, within Ac3H11_4986 at 150.787 kb on - strand, within Ac3H11_4986 at 150.787 kb on - strand, within Ac3H11_4986 at 150.789 kb on + strand, within Ac3H11_4986 at 150.789 kb on + strand, within Ac3H11_4986 at 150.790 kb on - strand, within Ac3H11_4986 at 150.790 kb on - strand, within Ac3H11_4986 at 150.790 kb on - strand, within Ac3H11_4986 at 150.790 kb on - strand, within Ac3H11_4986 at 150.790 kb on - strand, within Ac3H11_4986 at 150.858 kb on + strand, within Ac3H11_4986 at 150.858 kb on + strand, within Ac3H11_4986 at 150.859 kb on - strand, within Ac3H11_4986 at 151.171 kb on + strand at 151.171 kb on + strand at 151.171 kb on + strand at 151.171 kb on + strand at 151.171 kb on + strand at 151.172 kb on - strand at 151.172 kb on - strand at 151.172 kb on - strand at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.462 kb on + strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.463 kb on - strand, within Ac3H11_4987 at 151.485 kb on + strand, within Ac3H11_4987 at 151.485 kb on + strand, within Ac3H11_4987 at 151.485 kb on + strand, within Ac3H11_4987 at 151.486 kb on - strand, within Ac3H11_4987 at 151.486 kb on - strand, within Ac3H11_4987 at 151.510 kb on + strand, within Ac3H11_4987 at 151.510 kb on + strand, within Ac3H11_4987 at 151.510 kb on + strand, within Ac3H11_4987 at 151.511 kb on - strand, within Ac3H11_4987 at 151.511 kb on - strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.516 kb on + strand, within Ac3H11_4987 at 151.517 kb on - strand, within Ac3H11_4987 at 151.559 kb on - strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.676 kb on + strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.677 kb on - strand, within Ac3H11_4987 at 151.774 kb on + strand, within Ac3H11_4987 at 151.775 kb on - strand, within Ac3H11_4987 at 151.852 kb on + strand, within Ac3H11_4987 at 151.906 kb on + strand at 151.906 kb on + strand at 151.907 kb on - strand at 151.907 kb on - strand at 151.907 kb on - strand at 151.907 kb on - strand at 151.907 kb on - strand at 152.055 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 149,003 - Ac3H11_4985 0.13 +0.9 149,116 + Ac3H11_4985 0.24 +0.4 149,117 - Ac3H11_4985 0.24 +1.0 149,117 - Ac3H11_4985 0.24 +0.4 149,117 - Ac3H11_4985 0.24 -0.3 149,242 + Ac3H11_4985 0.36 -1.2 149,243 - Ac3H11_4985 0.36 +1.1 149,243 - Ac3H11_4985 0.36 -0.5 149,243 - Ac3H11_4985 0.36 -0.3 149,383 + Ac3H11_4985 0.50 -0.9 149,506 - Ac3H11_4985 0.62 -0.2 149,507 + Ac3H11_4985 0.62 +0.9 149,507 + Ac3H11_4985 0.62 +1.1 149,507 + Ac3H11_4985 0.62 -0.4 149,508 - Ac3H11_4985 0.62 +0.5 149,521 + Ac3H11_4985 0.63 +0.1 149,521 + Ac3H11_4985 0.63 +0.5 149,555 - Ac3H11_4985 0.66 -0.8 149,555 - Ac3H11_4985 0.66 +1.1 149,555 - Ac3H11_4985 0.66 -0.1 149,584 + Ac3H11_4985 0.69 +0.2 149,585 - Ac3H11_4985 0.69 -0.3 149,798 + Ac3H11_4985 0.90 +0.9 149,798 + Ac3H11_4985 0.90 -2.4 149,799 - Ac3H11_4985 0.90 -0.7 149,799 - Ac3H11_4985 0.90 -0.0 149,799 - Ac3H11_4985 0.90 +0.4 149,902 + +0.7 150,159 + Ac3H11_4986 0.20 -0.4 150,159 + Ac3H11_4986 0.20 -1.0 150,159 + Ac3H11_4986 0.20 +1.3 150,159 + Ac3H11_4986 0.20 -0.2 150,159 + Ac3H11_4986 0.20 -0.9 150,159 + Ac3H11_4986 0.20 +0.4 150,159 + Ac3H11_4986 0.20 -0.4 150,160 - Ac3H11_4986 0.20 +0.2 150,160 - Ac3H11_4986 0.20 -0.1 150,160 - Ac3H11_4986 0.20 -0.2 150,160 - Ac3H11_4986 0.20 +1.4 150,181 - Ac3H11_4986 0.22 -0.0 150,181 - Ac3H11_4986 0.22 -0.2 150,181 - Ac3H11_4986 0.22 -1.8 150,244 - Ac3H11_4986 0.27 -0.6 150,247 - Ac3H11_4986 0.27 -0.9 150,357 + Ac3H11_4986 0.37 -0.7 150,358 - Ac3H11_4986 0.37 +0.2 150,460 - Ac3H11_4986 0.46 +0.3 150,612 + Ac3H11_4986 0.58 +0.6 150,613 - Ac3H11_4986 0.58 +0.4 150,630 + Ac3H11_4986 0.60 +0.3 150,631 - Ac3H11_4986 0.60 -0.5 150,631 - Ac3H11_4986 0.60 +0.2 150,631 - Ac3H11_4986 0.60 -0.0 150,631 - Ac3H11_4986 0.60 +0.0 150,631 - Ac3H11_4986 0.60 -0.4 150,631 - Ac3H11_4986 0.60 -0.1 150,783 + Ac3H11_4986 0.73 -0.1 150,784 - Ac3H11_4986 0.73 +0.6 150,784 - Ac3H11_4986 0.73 -0.1 150,786 + Ac3H11_4986 0.73 -0.8 150,787 - Ac3H11_4986 0.73 +0.2 150,787 - Ac3H11_4986 0.73 -0.4 150,789 + Ac3H11_4986 0.73 +0.0 150,789 + Ac3H11_4986 0.73 -0.5 150,790 - Ac3H11_4986 0.73 -0.5 150,790 - Ac3H11_4986 0.73 +0.1 150,790 - Ac3H11_4986 0.73 -0.6 150,790 - Ac3H11_4986 0.73 -0.0 150,790 - Ac3H11_4986 0.73 +0.2 150,858 + Ac3H11_4986 0.79 +1.0 150,858 + Ac3H11_4986 0.79 +0.4 150,859 - Ac3H11_4986 0.79 +0.3 151,171 + +1.7 151,171 + +0.1 151,171 + -1.3 151,171 + -0.7 151,171 + +0.8 151,172 - +0.6 151,172 - +0.4 151,172 - +0.0 151,462 + Ac3H11_4987 0.42 +0.2 151,462 + Ac3H11_4987 0.42 +0.6 151,462 + Ac3H11_4987 0.42 -1.2 151,462 + Ac3H11_4987 0.42 -0.3 151,462 + Ac3H11_4987 0.42 +0.5 151,462 + Ac3H11_4987 0.42 +1.3 151,462 + Ac3H11_4987 0.42 +0.9 151,463 - Ac3H11_4987 0.42 +0.4 151,463 - Ac3H11_4987 0.42 +1.2 151,463 - Ac3H11_4987 0.42 +0.4 151,463 - Ac3H11_4987 0.42 -1.0 151,463 - Ac3H11_4987 0.42 -1.3 151,463 - Ac3H11_4987 0.42 +0.2 151,463 - Ac3H11_4987 0.42 +0.2 151,485 + Ac3H11_4987 0.45 -0.2 151,485 + Ac3H11_4987 0.45 +0.0 151,485 + Ac3H11_4987 0.45 -0.6 151,486 - Ac3H11_4987 0.45 +1.9 151,486 - Ac3H11_4987 0.45 -0.8 151,510 + Ac3H11_4987 0.48 +2.0 151,510 + Ac3H11_4987 0.48 -1.4 151,510 + Ac3H11_4987 0.48 -1.5 151,511 - Ac3H11_4987 0.48 -2.0 151,511 - Ac3H11_4987 0.48 -0.1 151,516 + Ac3H11_4987 0.49 -0.1 151,516 + Ac3H11_4987 0.49 +1.8 151,516 + Ac3H11_4987 0.49 -0.9 151,516 + Ac3H11_4987 0.49 +2.1 151,516 + Ac3H11_4987 0.49 -0.3 151,516 + Ac3H11_4987 0.49 -2.1 151,516 + Ac3H11_4987 0.49 +0.1 151,517 - Ac3H11_4987 0.49 -1.6 151,559 - Ac3H11_4987 0.54 -0.2 151,676 + Ac3H11_4987 0.68 +1.3 151,676 + Ac3H11_4987 0.68 +0.5 151,676 + Ac3H11_4987 0.68 -0.1 151,676 + Ac3H11_4987 0.68 -0.1 151,676 + Ac3H11_4987 0.68 -0.3 151,676 + Ac3H11_4987 0.68 +1.0 151,676 + Ac3H11_4987 0.68 +0.2 151,677 - Ac3H11_4987 0.68 +0.3 151,677 - Ac3H11_4987 0.68 +0.1 151,677 - Ac3H11_4987 0.68 +0.8 151,677 - Ac3H11_4987 0.68 -1.3 151,677 - Ac3H11_4987 0.68 -0.6 151,677 - Ac3H11_4987 0.68 -1.0 151,677 - Ac3H11_4987 0.68 +0.4 151,677 - Ac3H11_4987 0.68 -1.2 151,677 - Ac3H11_4987 0.68 -0.5 151,677 - Ac3H11_4987 0.68 -0.2 151,677 - Ac3H11_4987 0.68 +0.6 151,677 - Ac3H11_4987 0.68 -0.1 151,774 + Ac3H11_4987 0.80 +0.5 151,775 - Ac3H11_4987 0.80 -0.2 151,852 + Ac3H11_4987 0.90 +0.5 151,906 + +0.5 151,906 + +0.7 151,907 - +0.8 151,907 - +2.7 151,907 - -0.0 151,907 - +0.7 151,907 - -0.7 152,055 + +0.5
Or see this region's nucleotide sequence