Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_446 and Ac3H11_447 overlap by 39 nucleotides Ac3H11_447 and Ac3H11_448 overlap by 28 nucleotides
Ac3H11_446 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 133,320 to 135,545
_446
Ac3H11_447 - hypothetical protein, at 135,507 to 135,725
_447
Ac3H11_448 - Sensory box/GGDEF family protein, at 135,698 to 138,649
_448
Position (kb)
135
136 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 134.623 kb on + strand, within Ac3H11_446 at 134.624 kb on - strand, within Ac3H11_446 at 134.624 kb on - strand, within Ac3H11_446 at 134.638 kb on + strand, within Ac3H11_446 at 134.639 kb on - strand, within Ac3H11_446 at 134.691 kb on + strand, within Ac3H11_446 at 134.905 kb on + strand, within Ac3H11_446 at 134.905 kb on + strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.906 kb on - strand, within Ac3H11_446 at 134.933 kb on - strand, within Ac3H11_446 at 134.946 kb on + strand, within Ac3H11_446 at 134.946 kb on + strand, within Ac3H11_446 at 134.946 kb on + strand, within Ac3H11_446 at 134.946 kb on + strand, within Ac3H11_446 at 134.947 kb on - strand, within Ac3H11_446 at 134.947 kb on - strand, within Ac3H11_446 at 134.975 kb on - strand, within Ac3H11_446 at 134.999 kb on + strand, within Ac3H11_446 at 134.999 kb on + strand, within Ac3H11_446 at 134.999 kb on + strand, within Ac3H11_446 at 134.999 kb on + strand, within Ac3H11_446 at 134.999 kb on + strand, within Ac3H11_446 at 135.000 kb on - strand, within Ac3H11_446 at 135.413 kb on - strand at 135.459 kb on - strand at 135.462 kb on + strand at 135.462 kb on + strand at 135.462 kb on + strand at 135.462 kb on + strand at 135.462 kb on + strand at 135.462 kb on + strand at 135.462 kb on + strand at 135.463 kb on - strand at 135.463 kb on - strand at 135.463 kb on - strand at 135.463 kb on - strand at 135.463 kb on - strand at 135.463 kb on - strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.524 kb on + strand at 135.525 kb on - strand at 135.525 kb on - strand at 135.525 kb on - strand at 135.525 kb on - strand at 135.525 kb on - strand at 135.525 kb on - strand at 135.528 kb on + strand at 135.529 kb on - strand at 135.529 kb on - strand at 135.529 kb on - strand at 135.559 kb on + strand, within Ac3H11_447 at 135.559 kb on + strand, within Ac3H11_447 at 135.560 kb on - strand, within Ac3H11_447 at 135.572 kb on + strand, within Ac3H11_447 at 135.572 kb on + strand, within Ac3H11_447 at 135.572 kb on + strand, within Ac3H11_447 at 135.572 kb on + strand, within Ac3H11_447 at 135.572 kb on + strand, within Ac3H11_447 at 135.572 kb on + strand, within Ac3H11_447 at 135.572 kb on + strand, within Ac3H11_447 at 135.573 kb on - strand, within Ac3H11_447 at 135.573 kb on - strand, within Ac3H11_447 at 135.573 kb on - strand, within Ac3H11_447 at 135.573 kb on - strand, within Ac3H11_447 at 135.573 kb on - strand, within Ac3H11_447 at 135.573 kb on - strand, within Ac3H11_447 at 135.573 kb on - strand, within Ac3H11_447 at 135.582 kb on + strand, within Ac3H11_447 at 135.631 kb on + strand, within Ac3H11_447 at 135.645 kb on + strand, within Ac3H11_447 at 135.645 kb on + strand, within Ac3H11_447 at 135.645 kb on + strand, within Ac3H11_447 at 135.645 kb on + strand, within Ac3H11_447 at 135.645 kb on + strand, within Ac3H11_447 at 135.645 kb on + strand, within Ac3H11_447 at 135.646 kb on - strand, within Ac3H11_447 at 135.646 kb on - strand, within Ac3H11_447 at 135.646 kb on - strand, within Ac3H11_447 at 135.646 kb on - strand, within Ac3H11_447 at 135.646 kb on - strand, within Ac3H11_447 at 135.646 kb on - strand, within Ac3H11_447 at 135.653 kb on + strand, within Ac3H11_447 at 135.654 kb on - strand, within Ac3H11_447 at 135.662 kb on + strand, within Ac3H11_447 at 135.723 kb on + strand at 135.724 kb on - strand at 135.724 kb on - strand at 135.724 kb on - strand at 135.765 kb on + strand at 135.766 kb on - strand at 135.838 kb on + strand at 135.838 kb on + strand at 135.839 kb on - strand at 135.839 kb on - strand at 135.839 kb on - strand at 135.948 kb on + strand at 135.949 kb on - strand at 135.949 kb on - strand at 135.969 kb on + strand at 135.984 kb on + strand at 135.984 kb on + strand at 136.410 kb on + strand, within Ac3H11_448 at 136.411 kb on - strand, within Ac3H11_448 at 136.542 kb on + strand, within Ac3H11_448 at 136.542 kb on + strand, within Ac3H11_448 at 136.543 kb on - strand, within Ac3H11_448 at 136.566 kb on + strand, within Ac3H11_448 at 136.599 kb on + strand, within Ac3H11_448 at 136.638 kb on + strand, within Ac3H11_448 at 136.639 kb on - strand, within Ac3H11_448 at 136.646 kb on + strand, within Ac3H11_448 at 136.647 kb on - strand, within Ac3H11_448 at 136.647 kb on - strand, within Ac3H11_448 at 136.647 kb on - strand, within Ac3H11_448 at 136.664 kb on + strand, within Ac3H11_448 at 136.665 kb on - strand, within Ac3H11_448
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 134,623 + Ac3H11_446 0.59 +1.1 134,624 - Ac3H11_446 0.59 +1.2 134,624 - Ac3H11_446 0.59 +1.7 134,638 + Ac3H11_446 0.59 +2.3 134,639 - Ac3H11_446 0.59 -1.6 134,691 + Ac3H11_446 0.62 -0.2 134,905 + Ac3H11_446 0.71 +1.7 134,905 + Ac3H11_446 0.71 +1.7 134,906 - Ac3H11_446 0.71 +1.9 134,906 - Ac3H11_446 0.71 +0.2 134,906 - Ac3H11_446 0.71 +2.6 134,906 - Ac3H11_446 0.71 +0.7 134,906 - Ac3H11_446 0.71 +0.5 134,906 - Ac3H11_446 0.71 +1.2 134,906 - Ac3H11_446 0.71 +2.5 134,906 - Ac3H11_446 0.71 +1.7 134,906 - Ac3H11_446 0.71 +1.8 134,933 - Ac3H11_446 0.72 +1.3 134,946 + Ac3H11_446 0.73 +0.7 134,946 + Ac3H11_446 0.73 +0.5 134,946 + Ac3H11_446 0.73 +1.1 134,946 + Ac3H11_446 0.73 +1.5 134,947 - Ac3H11_446 0.73 +1.1 134,947 - Ac3H11_446 0.73 +2.3 134,975 - Ac3H11_446 0.74 +1.8 134,999 + Ac3H11_446 0.75 +1.4 134,999 + Ac3H11_446 0.75 +1.0 134,999 + Ac3H11_446 0.75 +2.0 134,999 + Ac3H11_446 0.75 +1.7 134,999 + Ac3H11_446 0.75 +2.1 135,000 - Ac3H11_446 0.75 +1.0 135,413 - -2.0 135,459 - +0.4 135,462 + +1.2 135,462 + +0.8 135,462 + +1.5 135,462 + +0.4 135,462 + +1.6 135,462 + +1.3 135,462 + +0.5 135,463 - +1.7 135,463 - +0.4 135,463 - +3.7 135,463 - -0.3 135,463 - -0.1 135,463 - -0.1 135,524 + +1.5 135,524 + +2.1 135,524 + +0.8 135,524 + +1.4 135,524 + +1.1 135,524 + +1.9 135,524 + +1.3 135,524 + +1.4 135,524 + +1.8 135,525 - -0.5 135,525 - -1.1 135,525 - +0.4 135,525 - +1.3 135,525 - +1.5 135,525 - -0.8 135,528 + +0.7 135,529 - +0.6 135,529 - +2.1 135,529 - +0.1 135,559 + Ac3H11_447 0.24 +1.4 135,559 + Ac3H11_447 0.24 +0.1 135,560 - Ac3H11_447 0.24 -0.7 135,572 + Ac3H11_447 0.30 +1.0 135,572 + Ac3H11_447 0.30 +0.6 135,572 + Ac3H11_447 0.30 +0.5 135,572 + Ac3H11_447 0.30 +1.0 135,572 + Ac3H11_447 0.30 +1.2 135,572 + Ac3H11_447 0.30 +2.2 135,572 + Ac3H11_447 0.30 +0.5 135,573 - Ac3H11_447 0.30 +1.3 135,573 - Ac3H11_447 0.30 +1.5 135,573 - Ac3H11_447 0.30 -1.0 135,573 - Ac3H11_447 0.30 -0.1 135,573 - Ac3H11_447 0.30 +1.0 135,573 - Ac3H11_447 0.30 +0.9 135,573 - Ac3H11_447 0.30 +0.4 135,582 + Ac3H11_447 0.34 +0.7 135,631 + Ac3H11_447 0.57 +0.0 135,645 + Ac3H11_447 0.63 +1.5 135,645 + Ac3H11_447 0.63 +1.0 135,645 + Ac3H11_447 0.63 +0.4 135,645 + Ac3H11_447 0.63 -2.8 135,645 + Ac3H11_447 0.63 +3.3 135,645 + Ac3H11_447 0.63 +0.2 135,646 - Ac3H11_447 0.63 -0.4 135,646 - Ac3H11_447 0.63 -2.3 135,646 - Ac3H11_447 0.63 -0.5 135,646 - Ac3H11_447 0.63 -0.6 135,646 - Ac3H11_447 0.63 -1.0 135,646 - Ac3H11_447 0.63 +0.3 135,653 + Ac3H11_447 0.67 +1.0 135,654 - Ac3H11_447 0.67 -0.8 135,662 + Ac3H11_447 0.71 -0.1 135,723 + -0.1 135,724 - -0.2 135,724 - -1.7 135,724 - -0.5 135,765 + -0.6 135,766 - -0.4 135,838 + -0.9 135,838 + -0.6 135,839 - -0.1 135,839 - +1.9 135,839 - -0.6 135,948 + +0.2 135,949 - -0.2 135,949 - +0.2 135,969 + -0.5 135,984 + +0.1 135,984 + -0.8 136,410 + Ac3H11_448 0.24 +0.5 136,411 - Ac3H11_448 0.24 -0.4 136,542 + Ac3H11_448 0.29 -0.1 136,542 + Ac3H11_448 0.29 +0.2 136,543 - Ac3H11_448 0.29 +1.3 136,566 + Ac3H11_448 0.29 -1.7 136,599 + Ac3H11_448 0.31 -1.5 136,638 + Ac3H11_448 0.32 -0.2 136,639 - Ac3H11_448 0.32 +0.5 136,646 + Ac3H11_448 0.32 -0.2 136,647 - Ac3H11_448 0.32 +0.7 136,647 - Ac3H11_448 0.32 +0.5 136,647 - Ac3H11_448 0.32 +2.1 136,664 + Ac3H11_448 0.33 -0.1 136,665 - Ac3H11_448 0.33 +0.8
Or see this region's nucleotide sequence