Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_3729

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_3727 and Ac3H11_3728 are separated by 29 nucleotidesAc3H11_3728 and Ac3H11_3729 overlap by 4 nucleotidesAc3H11_3729 and Ac3H11_3730 are separated by 23 nucleotidesAc3H11_3730 and Ac3H11_3731 are separated by 25 nucleotides Ac3H11_3727 - Creatinine amidohydrolase (EC 3.5.2.10), at 87,448 to 88,311 _3727 Ac3H11_3728 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 88,341 to 88,805 _3728 Ac3H11_3729 - Cytosine deaminase (EC 3.5.4.1), at 88,802 to 90,130 _3729 Ac3H11_3730 - Glutathione-regulated potassium-efflux system ancillary protein KefG, at 90,154 to 90,741 _3730 Ac3H11_3731 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 90,767 to 91,069 _3731 Position (kb) 88 89 90 91Strain fitness (log2 ratio) -2 -1 0 1 2 3at 87.981 kb on - strand, within Ac3H11_3727at 88.076 kb on + strand, within Ac3H11_3727at 88.076 kb on + strand, within Ac3H11_3727at 88.077 kb on - strand, within Ac3H11_3727at 88.077 kb on - strand, within Ac3H11_3727at 88.298 kb on + strandat 88.298 kb on + strandat 88.298 kb on + strandat 88.299 kb on - strandat 88.299 kb on - strandat 88.357 kb on + strandat 88.357 kb on + strandat 88.357 kb on + strandat 88.501 kb on + strand, within Ac3H11_3728at 88.501 kb on + strand, within Ac3H11_3728at 88.502 kb on - strand, within Ac3H11_3728at 88.502 kb on - strand, within Ac3H11_3728at 88.576 kb on + strand, within Ac3H11_3728at 88.576 kb on + strand, within Ac3H11_3728at 88.576 kb on + strand, within Ac3H11_3728at 88.577 kb on - strand, within Ac3H11_3728at 88.577 kb on - strand, within Ac3H11_3728at 88.577 kb on - strand, within Ac3H11_3728at 88.577 kb on - strand, within Ac3H11_3728at 88.727 kb on - strand, within Ac3H11_3728at 89.169 kb on - strand, within Ac3H11_3729at 89.169 kb on - strand, within Ac3H11_3729at 90.155 kb on + strandat 90.156 kb on - strandat 90.156 kb on - strandat 90.156 kb on - strandat 90.156 kb on - strandat 90.156 kb on - strandat 90.233 kb on + strand, within Ac3H11_3730at 90.233 kb on + strand, within Ac3H11_3730at 90.234 kb on - strand, within Ac3H11_3730at 90.237 kb on - strand, within Ac3H11_3730at 90.237 kb on - strand, within Ac3H11_3730at 90.245 kb on + strand, within Ac3H11_3730at 90.285 kb on - strand, within Ac3H11_3730at 90.285 kb on - strand, within Ac3H11_3730at 90.285 kb on - strand, within Ac3H11_3730at 90.348 kb on - strand, within Ac3H11_3730at 90.348 kb on - strand, within Ac3H11_3730at 90.348 kb on - strand, within Ac3H11_3730at 90.536 kb on + strand, within Ac3H11_3730at 90.537 kb on - strand, within Ac3H11_3730at 90.623 kb on + strand, within Ac3H11_3730at 90.699 kb on - strandat 90.710 kb on - strandat 90.722 kb on + strandat 90.722 kb on + strandat 90.722 kb on + strandat 90.723 kb on - strandat 90.723 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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87,981 - Ac3H11_3727 0.62 +0.1
88,076 + Ac3H11_3727 0.73 -0.8
88,076 + Ac3H11_3727 0.73 -0.4
88,077 - Ac3H11_3727 0.73 +0.4
88,077 - Ac3H11_3727 0.73 -0.9
88,298 + -0.5
88,298 + -0.3
88,298 + +1.1
88,299 - +1.4
88,299 - -0.7
88,357 + -1.2
88,357 + +0.1
88,357 + -0.0
88,501 + Ac3H11_3728 0.34 -0.3
88,501 + Ac3H11_3728 0.34 -0.5
88,502 - Ac3H11_3728 0.35 +0.3
88,502 - Ac3H11_3728 0.35 +0.1
88,576 + Ac3H11_3728 0.51 -0.3
88,576 + Ac3H11_3728 0.51 +0.1
88,576 + Ac3H11_3728 0.51 +0.5
88,577 - Ac3H11_3728 0.51 -0.7
88,577 - Ac3H11_3728 0.51 -0.4
88,577 - Ac3H11_3728 0.51 -0.2
88,577 - Ac3H11_3728 0.51 -0.4
88,727 - Ac3H11_3728 0.83 +0.7
89,169 - Ac3H11_3729 0.28 +0.2
89,169 - Ac3H11_3729 0.28 +1.2
90,155 + -0.0
90,156 - -1.2
90,156 - +2.9
90,156 - -0.3
90,156 - +0.1
90,156 - -0.4
90,233 + Ac3H11_3730 0.13 -2.1
90,233 + Ac3H11_3730 0.13 -0.2
90,234 - Ac3H11_3730 0.14 -0.7
90,237 - Ac3H11_3730 0.14 +1.6
90,237 - Ac3H11_3730 0.14 -0.5
90,245 + Ac3H11_3730 0.15 -1.3
90,285 - Ac3H11_3730 0.22 -0.1
90,285 - Ac3H11_3730 0.22 +0.2
90,285 - Ac3H11_3730 0.22 +0.1
90,348 - Ac3H11_3730 0.33 -1.6
90,348 - Ac3H11_3730 0.33 -0.8
90,348 - Ac3H11_3730 0.33 -2.0
90,536 + Ac3H11_3730 0.65 -0.5
90,537 - Ac3H11_3730 0.65 -1.0
90,623 + Ac3H11_3730 0.80 -0.9
90,699 - -0.2
90,710 - -0.5
90,722 + +3.7
90,722 + +0.1
90,722 + -0.4
90,723 - -0.7
90,723 - +0.7

Or see this region's nucleotide sequence