Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2272 and Ac3H11_2273 are separated by 3 nucleotides Ac3H11_2273 and Ac3H11_2274 are separated by 156 nucleotides Ac3H11_2274 and Ac3H11_2275 are separated by 119 nucleotides
Ac3H11_2272 - Biotin synthase (EC 2.8.1.6), at 506,810 to 507,886
_2272
Ac3H11_2273 - hypothetical protein, at 507,890 to 508,162
_2273
Ac3H11_2274 - Methylmalonyl-CoA epimerase (EC 5.1.99.1), at 508,319 to 508,789
_2274
Ac3H11_2275 - Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3), at 508,909 to 510,957
_2275
Position (kb) 507
508
509 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 506.938 kb on - strand, within Ac3H11_2272 at 506.938 kb on - strand, within Ac3H11_2272 at 507.285 kb on + strand, within Ac3H11_2272 at 507.286 kb on - strand, within Ac3H11_2272 at 507.312 kb on + strand, within Ac3H11_2272 at 507.312 kb on + strand, within Ac3H11_2272 at 507.312 kb on + strand, within Ac3H11_2272 at 507.312 kb on + strand, within Ac3H11_2272 at 507.313 kb on - strand, within Ac3H11_2272 at 507.313 kb on - strand, within Ac3H11_2272 at 507.316 kb on - strand, within Ac3H11_2272 at 507.316 kb on - strand, within Ac3H11_2272 at 507.316 kb on - strand, within Ac3H11_2272 at 507.345 kb on + strand, within Ac3H11_2272 at 507.345 kb on + strand, within Ac3H11_2272 at 507.345 kb on + strand, within Ac3H11_2272 at 507.345 kb on + strand, within Ac3H11_2272 at 507.345 kb on + strand, within Ac3H11_2272 at 507.345 kb on + strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.346 kb on - strand, within Ac3H11_2272 at 507.348 kb on + strand, within Ac3H11_2272 at 507.349 kb on - strand, within Ac3H11_2272 at 507.349 kb on - strand, within Ac3H11_2272 at 507.906 kb on + strand at 507.956 kb on + strand, within Ac3H11_2273 at 507.956 kb on + strand, within Ac3H11_2273 at 507.956 kb on + strand, within Ac3H11_2273 at 507.957 kb on - strand, within Ac3H11_2273 at 508.051 kb on - strand, within Ac3H11_2273 at 508.192 kb on - strand at 508.210 kb on + strand at 508.210 kb on + strand at 508.210 kb on + strand at 508.211 kb on - strand at 508.211 kb on - strand at 508.211 kb on - strand at 508.217 kb on - strand at 508.217 kb on - strand at 508.217 kb on - strand at 508.431 kb on + strand, within Ac3H11_2274 at 508.794 kb on - strand at 508.811 kb on + strand at 508.812 kb on - strand at 508.812 kb on - strand at 509.029 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 506,938 - Ac3H11_2272 0.12 -0.2 506,938 - Ac3H11_2272 0.12 -0.7 507,285 + Ac3H11_2272 0.44 +0.4 507,286 - Ac3H11_2272 0.44 -2.9 507,312 + Ac3H11_2272 0.47 -1.2 507,312 + Ac3H11_2272 0.47 -0.7 507,312 + Ac3H11_2272 0.47 -2.2 507,312 + Ac3H11_2272 0.47 -0.3 507,313 - Ac3H11_2272 0.47 -0.2 507,313 - Ac3H11_2272 0.47 +0.3 507,316 - Ac3H11_2272 0.47 -0.5 507,316 - Ac3H11_2272 0.47 +0.6 507,316 - Ac3H11_2272 0.47 -0.9 507,345 + Ac3H11_2272 0.50 +0.8 507,345 + Ac3H11_2272 0.50 +2.4 507,345 + Ac3H11_2272 0.50 -0.1 507,345 + Ac3H11_2272 0.50 -0.5 507,345 + Ac3H11_2272 0.50 -1.0 507,345 + Ac3H11_2272 0.50 -0.0 507,346 - Ac3H11_2272 0.50 -0.5 507,346 - Ac3H11_2272 0.50 -0.6 507,346 - Ac3H11_2272 0.50 -0.9 507,346 - Ac3H11_2272 0.50 -0.2 507,346 - Ac3H11_2272 0.50 +0.9 507,346 - Ac3H11_2272 0.50 -0.9 507,346 - Ac3H11_2272 0.50 +0.2 507,346 - Ac3H11_2272 0.50 -0.5 507,346 - Ac3H11_2272 0.50 +0.6 507,346 - Ac3H11_2272 0.50 -0.2 507,346 - Ac3H11_2272 0.50 -0.4 507,346 - Ac3H11_2272 0.50 -0.8 507,348 + Ac3H11_2272 0.50 -0.8 507,349 - Ac3H11_2272 0.50 -1.0 507,349 - Ac3H11_2272 0.50 -0.6 507,906 + -1.0 507,956 + Ac3H11_2273 0.24 -0.9 507,956 + Ac3H11_2273 0.24 -0.5 507,956 + Ac3H11_2273 0.24 -1.3 507,957 - Ac3H11_2273 0.25 +1.9 508,051 - Ac3H11_2273 0.59 +0.6 508,192 - +0.2 508,210 + +0.2 508,210 + +0.5 508,210 + +0.5 508,211 - -1.4 508,211 - +0.8 508,211 - -0.4 508,217 - -2.1 508,217 - -0.1 508,217 - -2.2 508,431 + Ac3H11_2274 0.24 -2.1 508,794 - +0.2 508,811 + -0.4 508,812 - +0.3 508,812 - +1.2 509,029 + -1.2
Or see this region's nucleotide sequence