Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1820 and Ac3H11_1821 are separated by 49 nucleotides Ac3H11_1821 and Ac3H11_1822 are separated by 33 nucleotides Ac3H11_1822 and Ac3H11_1823 are separated by 12 nucleotides Ac3H11_1823 and Ac3H11_1824 are separated by 23 nucleotides
Ac3H11_1820 - COG1565: Uncharacterized conserved protein, at 24,479 to 25,597
_1820
Ac3H11_1821 - hypothetical protein, at 25,647 to 25,805
_1821
Ac3H11_1822 - Thioredoxin, at 25,839 to 26,306
_1822
Ac3H11_1823 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 26,319 to 26,792
_1823
Ac3H11_1824 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 26,816 to 27,577
_1824
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 25.645 kb on + strand at 25.645 kb on + strand at 25.646 kb on - strand at 25.646 kb on - strand at 25.803 kb on + strand at 25.803 kb on + strand at 25.803 kb on + strand at 25.804 kb on - strand at 25.980 kb on - strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.187 kb on + strand, within Ac3H11_1822 at 26.304 kb on + strand at 26.305 kb on - strand at 26.377 kb on + strand, within Ac3H11_1823 at 26.377 kb on + strand, within Ac3H11_1823 at 26.377 kb on + strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.378 kb on - strand, within Ac3H11_1823 at 26.425 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.575 kb on + strand, within Ac3H11_1823 at 26.576 kb on - strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.596 kb on + strand, within Ac3H11_1823 at 26.597 kb on - strand, within Ac3H11_1823 at 27.267 kb on + strand, within Ac3H11_1824 at 27.267 kb on + strand, within Ac3H11_1824 at 27.267 kb on + strand, within Ac3H11_1824 at 27.268 kb on - strand, within Ac3H11_1824 at 27.268 kb on - strand, within Ac3H11_1824
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 25,645 + -0.6 25,645 + -1.4 25,646 - -0.1 25,646 - +1.0 25,803 + -1.7 25,803 + -0.4 25,803 + +0.4 25,804 - +0.4 25,980 - Ac3H11_1822 0.30 -0.8 26,187 + Ac3H11_1822 0.74 -0.2 26,187 + Ac3H11_1822 0.74 +0.1 26,187 + Ac3H11_1822 0.74 +0.6 26,187 + Ac3H11_1822 0.74 +0.0 26,187 + Ac3H11_1822 0.74 -0.4 26,304 + +0.9 26,305 - -0.6 26,377 + Ac3H11_1823 0.12 -1.0 26,377 + Ac3H11_1823 0.12 +1.5 26,377 + Ac3H11_1823 0.12 +1.3 26,378 - Ac3H11_1823 0.12 +1.7 26,378 - Ac3H11_1823 0.12 +0.1 26,378 - Ac3H11_1823 0.12 -0.7 26,425 + Ac3H11_1823 0.22 +0.9 26,575 + Ac3H11_1823 0.54 -0.4 26,575 + Ac3H11_1823 0.54 +0.5 26,575 + Ac3H11_1823 0.54 +1.0 26,575 + Ac3H11_1823 0.54 +1.4 26,575 + Ac3H11_1823 0.54 -0.7 26,576 - Ac3H11_1823 0.54 -0.4 26,596 + Ac3H11_1823 0.58 +0.3 26,596 + Ac3H11_1823 0.58 -1.9 26,596 + Ac3H11_1823 0.58 +0.4 26,597 - Ac3H11_1823 0.59 -1.0 27,267 + Ac3H11_1824 0.59 -1.3 27,267 + Ac3H11_1824 0.59 -0.2 27,267 + Ac3H11_1824 0.59 +0.0 27,268 - Ac3H11_1824 0.59 +0.1 27,268 - Ac3H11_1824 0.59 +0.0
Or see this region's nucleotide sequence