Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_181

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_179 and Ac3H11_180 are separated by 196 nucleotidesAc3H11_180 and Ac3H11_181 are separated by 138 nucleotidesAc3H11_181 and Ac3H11_182 are separated by 144 nucleotidesAc3H11_182 and Ac3H11_183 are separated by 301 nucleotides Ac3H11_179 - Tricarboxylate transport protein TctC, at 67,624 to 68,607 _179 Ac3H11_180 - Universal stress protein family, at 68,804 to 69,241 _180 Ac3H11_181 - FIG00538743: hypothetical protein, at 69,380 to 70,156 _181 Ac3H11_182 - hypothetical protein, at 70,301 to 70,789 _182 Ac3H11_183 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 71,091 to 74,063 _183 Position (kb) 69 70 71Strain fitness (log2 ratio) -2 -1 0 1 2at 68.390 kb on - strand, within Ac3H11_179at 68.557 kb on + strandat 68.557 kb on + strandat 68.557 kb on + strandat 68.558 kb on - strandat 68.558 kb on - strandat 68.558 kb on - strandat 68.558 kb on - strandat 68.733 kb on - strandat 68.733 kb on - strandat 69.069 kb on + strand, within Ac3H11_180at 69.069 kb on + strand, within Ac3H11_180at 69.069 kb on + strand, within Ac3H11_180at 69.070 kb on - strand, within Ac3H11_180at 69.070 kb on - strand, within Ac3H11_180at 69.070 kb on - strand, within Ac3H11_180at 69.070 kb on - strand, within Ac3H11_180at 69.070 kb on - strand, within Ac3H11_180at 69.070 kb on - strand, within Ac3H11_180at 69.084 kb on + strand, within Ac3H11_180at 69.132 kb on + strand, within Ac3H11_180at 69.132 kb on + strand, within Ac3H11_180at 69.132 kb on + strand, within Ac3H11_180at 69.132 kb on + strand, within Ac3H11_180at 69.132 kb on + strand, within Ac3H11_180at 69.132 kb on + strand, within Ac3H11_180at 69.133 kb on - strand, within Ac3H11_180at 69.133 kb on - strand, within Ac3H11_180at 69.133 kb on - strand, within Ac3H11_180at 69.133 kb on - strand, within Ac3H11_180at 69.133 kb on - strand, within Ac3H11_180at 69.247 kb on + strandat 69.247 kb on + strandat 69.247 kb on + strandat 69.247 kb on + strandat 69.247 kb on + strandat 69.248 kb on - strandat 69.248 kb on - strandat 69.248 kb on - strandat 69.248 kb on - strandat 69.248 kb on - strandat 69.254 kb on + strandat 69.255 kb on - strandat 69.255 kb on - strandat 69.255 kb on - strandat 69.255 kb on - strandat 69.277 kb on + strandat 69.277 kb on + strandat 69.278 kb on - strandat 69.278 kb on - strandat 69.278 kb on - strandat 69.333 kb on - strandat 69.874 kb on - strand, within Ac3H11_181at 69.874 kb on - strand, within Ac3H11_181at 69.874 kb on - strand, within Ac3H11_181at 69.874 kb on - strand, within Ac3H11_181at 70.073 kb on + strand, within Ac3H11_181at 70.083 kb on + strandat 70.083 kb on + strandat 70.157 kb on - strandat 70.170 kb on + strandat 70.170 kb on + strandat 70.170 kb on + strandat 70.506 kb on + strand, within Ac3H11_182at 70.506 kb on + strand, within Ac3H11_182at 70.506 kb on + strand, within Ac3H11_182at 70.506 kb on + strand, within Ac3H11_182at 70.507 kb on - strand, within Ac3H11_182at 70.507 kb on - strand, within Ac3H11_182at 70.507 kb on - strand, within Ac3H11_182at 70.531 kb on - strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.578 kb on + strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.579 kb on - strand, within Ac3H11_182at 70.584 kb on + strand, within Ac3H11_182at 70.584 kb on + strand, within Ac3H11_182at 70.584 kb on + strand, within Ac3H11_182at 70.584 kb on + strand, within Ac3H11_182at 70.584 kb on + strand, within Ac3H11_182at 70.584 kb on + strand, within Ac3H11_182at 70.585 kb on - strand, within Ac3H11_182at 70.585 kb on - strand, within Ac3H11_182at 70.585 kb on - strand, within Ac3H11_182at 70.686 kb on + strand, within Ac3H11_182at 70.686 kb on + strand, within Ac3H11_182at 70.687 kb on - strand, within Ac3H11_182at 70.728 kb on + strand, within Ac3H11_182at 70.728 kb on + strand, within Ac3H11_182at 70.728 kb on + strandat 70.728 kb on + strand, within Ac3H11_182at 70.728 kb on + strand, within Ac3H11_182at 70.728 kb on + strand, within Ac3H11_182at 70.728 kb on + strand, within Ac3H11_182at 70.728 kb on + strand, within Ac3H11_182at 70.728 kb on + strand, within Ac3H11_182at 70.729 kb on - strand, within Ac3H11_182at 70.729 kb on - strand, within Ac3H11_182at 70.729 kb on - strand, within Ac3H11_182at 70.729 kb on - strand, within Ac3H11_182at 70.729 kb on - strand, within Ac3H11_182at 70.729 kb on - strand, within Ac3H11_182at 70.729 kb on - strand, within Ac3H11_182at 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.828 kb on + strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.829 kb on - strandat 70.942 kb on + strandat 71.004 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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68,390 - Ac3H11_179 0.78 -0.3
68,557 + -0.0
68,557 + +1.1
68,557 + +0.5
68,558 - +1.2
68,558 - -0.1
68,558 - -0.6
68,558 - -0.3
68,733 - +0.7
68,733 - -0.8
69,069 + Ac3H11_180 0.61 +0.5
69,069 + Ac3H11_180 0.61 -0.0
69,069 + Ac3H11_180 0.61 +0.4
69,070 - Ac3H11_180 0.61 +0.1
69,070 - Ac3H11_180 0.61 -1.6
69,070 - Ac3H11_180 0.61 +1.2
69,070 - Ac3H11_180 0.61 -0.7
69,070 - Ac3H11_180 0.61 +0.3
69,070 - Ac3H11_180 0.61 -0.9
69,084 + Ac3H11_180 0.64 -0.3
69,132 + Ac3H11_180 0.75 +1.1
69,132 + Ac3H11_180 0.75 -0.3
69,132 + Ac3H11_180 0.75 -0.2
69,132 + Ac3H11_180 0.75 +0.4
69,132 + Ac3H11_180 0.75 -1.7
69,132 + Ac3H11_180 0.75 -1.0
69,133 - Ac3H11_180 0.75 +0.2
69,133 - Ac3H11_180 0.75 -1.1
69,133 - Ac3H11_180 0.75 -0.2
69,133 - Ac3H11_180 0.75 -1.2
69,133 - Ac3H11_180 0.75 +0.4
69,247 + +0.5
69,247 + +0.2
69,247 + -0.1
69,247 + -0.5
69,247 + +1.3
69,248 - +0.7
69,248 - +0.6
69,248 - -2.2
69,248 - -0.7
69,248 - +0.4
69,254 + +0.0
69,255 - +1.3
69,255 - -0.8
69,255 - -1.5
69,255 - +0.8
69,277 + +0.2
69,277 + +0.4
69,278 - +0.1
69,278 - +1.2
69,278 - +0.0
69,333 - -1.0
69,874 - Ac3H11_181 0.64 +0.7
69,874 - Ac3H11_181 0.64 -0.6
69,874 - Ac3H11_181 0.64 +0.4
69,874 - Ac3H11_181 0.64 +0.9
70,073 + Ac3H11_181 0.89 +0.8
70,083 + +0.2
70,083 + -0.9
70,157 - -0.5
70,170 + +0.1
70,170 + -1.1
70,170 + +0.3
70,506 + Ac3H11_182 0.42 +0.2
70,506 + Ac3H11_182 0.42 -0.5
70,506 + Ac3H11_182 0.42 -0.1
70,506 + Ac3H11_182 0.42 +0.9
70,507 - Ac3H11_182 0.42 +0.7
70,507 - Ac3H11_182 0.42 +0.5
70,507 - Ac3H11_182 0.42 -2.0
70,531 - Ac3H11_182 0.47 -0.3
70,578 + Ac3H11_182 0.57 +0.4
70,578 + Ac3H11_182 0.57 +0.1
70,578 + Ac3H11_182 0.57 -1.4
70,578 + Ac3H11_182 0.57 +0.4
70,578 + Ac3H11_182 0.57 -0.6
70,578 + Ac3H11_182 0.57 -0.0
70,578 + Ac3H11_182 0.57 +1.0
70,578 + Ac3H11_182 0.57 -0.3
70,578 + Ac3H11_182 0.57 -0.3
70,578 + Ac3H11_182 0.57 +0.5
70,578 + Ac3H11_182 0.57 +0.8
70,578 + Ac3H11_182 0.57 -1.1
70,579 - Ac3H11_182 0.57 -0.3
70,579 - Ac3H11_182 0.57 -0.1
70,579 - Ac3H11_182 0.57 -0.0
70,579 - Ac3H11_182 0.57 +0.9
70,579 - Ac3H11_182 0.57 -0.2
70,579 - Ac3H11_182 0.57 -0.7
70,579 - Ac3H11_182 0.57 -0.9
70,579 - Ac3H11_182 0.57 +0.0
70,579 - Ac3H11_182 0.57 +0.4
70,579 - Ac3H11_182 0.57 -1.8
70,579 - Ac3H11_182 0.57 -1.0
70,579 - Ac3H11_182 0.57 +0.6
70,579 - Ac3H11_182 0.57 +0.0
70,584 + Ac3H11_182 0.58 -0.6
70,584 + Ac3H11_182 0.58 +0.8
70,584 + Ac3H11_182 0.58 +0.3
70,584 + Ac3H11_182 0.58 -0.2
70,584 + Ac3H11_182 0.58 +0.8
70,584 + Ac3H11_182 0.58 +1.6
70,585 - Ac3H11_182 0.58 -0.5
70,585 - Ac3H11_182 0.58 +0.8
70,585 - Ac3H11_182 0.58 -0.9
70,686 + Ac3H11_182 0.79 +1.4
70,686 + Ac3H11_182 0.79 +0.8
70,687 - Ac3H11_182 0.79 +1.3
70,728 + Ac3H11_182 0.87 +0.3
70,728 + Ac3H11_182 0.87 +0.2
70,728 + +2.0
70,728 + Ac3H11_182 0.87 +1.2
70,728 + Ac3H11_182 0.87 +0.6
70,728 + Ac3H11_182 0.87 +1.5
70,728 + Ac3H11_182 0.87 +0.1
70,728 + Ac3H11_182 0.87 +1.2
70,728 + Ac3H11_182 0.87 -1.2
70,729 - Ac3H11_182 0.88 +0.2
70,729 - Ac3H11_182 0.88 -0.5
70,729 - Ac3H11_182 0.88 -0.4
70,729 - Ac3H11_182 0.88 +1.0
70,729 - Ac3H11_182 0.88 -0.3
70,729 - Ac3H11_182 0.88 -1.1
70,729 - Ac3H11_182 0.88 -1.5
70,828 + +0.5
70,828 + -1.1
70,828 + +1.8
70,828 + +0.9
70,828 + +1.0
70,828 + +1.7
70,828 + +0.7
70,828 + +0.0
70,828 + -1.7
70,828 + -0.3
70,828 + -0.7
70,829 - +1.5
70,829 - -0.4
70,829 - -0.1
70,829 - -1.8
70,829 - -0.6
70,829 - -0.4
70,829 - +0.5
70,829 - -0.4
70,829 - -0.8
70,829 - +0.1
70,829 - -0.7
70,942 + -1.0
71,004 - +0.8

Or see this region's nucleotide sequence