Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1222 and Ac3H11_1223 are separated by 6 nucleotides Ac3H11_1223 and Ac3H11_1224 are separated by 18 nucleotides Ac3H11_1224 and Ac3H11_1225 are separated by 15 nucleotides Ac3H11_1225 and Ac3H11_1226 are separated by 36 nucleotides
Ac3H11_1222 - Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-), at 40,823 to 41,587
_1222
Ac3H11_1223 - FIG002994: Putative transcriptional regulator, at 41,594 to 42,049
_1223
Ac3H11_1224 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 42,068 to 42,973
_1224
Ac3H11_1225 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 42,989 to 43,888
_1225
Ac3H11_1226 - Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25), at 43,925 to 44,836
_1226
Position (kb) 42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 41.072 kb on + strand, within Ac3H11_1222 at 41.072 kb on + strand, within Ac3H11_1222 at 41.072 kb on + strand, within Ac3H11_1222 at 41.072 kb on + strand, within Ac3H11_1222 at 41.085 kb on - strand, within Ac3H11_1222 at 41.087 kb on + strand, within Ac3H11_1222 at 41.088 kb on - strand, within Ac3H11_1222 at 41.150 kb on + strand, within Ac3H11_1222 at 41.150 kb on + strand, within Ac3H11_1222 at 41.290 kb on + strand, within Ac3H11_1222 at 41.328 kb on - strand, within Ac3H11_1222 at 41.510 kb on - strand, within Ac3H11_1222 at 41.510 kb on - strand, within Ac3H11_1222 at 41.510 kb on - strand, within Ac3H11_1222 at 41.552 kb on + strand at 41.552 kb on + strand at 41.606 kb on + strand at 41.606 kb on + strand at 41.679 kb on + strand, within Ac3H11_1223 at 41.679 kb on + strand, within Ac3H11_1223 at 41.680 kb on - strand, within Ac3H11_1223 at 41.680 kb on - strand, within Ac3H11_1223 at 41.699 kb on + strand, within Ac3H11_1223 at 41.700 kb on - strand, within Ac3H11_1223 at 41.748 kb on + strand, within Ac3H11_1223 at 41.748 kb on + strand, within Ac3H11_1223 at 41.748 kb on + strand, within Ac3H11_1223 at 41.963 kb on + strand, within Ac3H11_1223 at 41.964 kb on - strand, within Ac3H11_1223 at 41.987 kb on + strand, within Ac3H11_1223 at 42.122 kb on + strand at 42.123 kb on - strand at 42.171 kb on + strand, within Ac3H11_1224 at 42.172 kb on - strand, within Ac3H11_1224 at 42.192 kb on + strand, within Ac3H11_1224 at 42.232 kb on - strand, within Ac3H11_1224 at 42.254 kb on + strand, within Ac3H11_1224 at 42.381 kb on + strand, within Ac3H11_1224 at 42.381 kb on + strand, within Ac3H11_1224 at 42.382 kb on - strand, within Ac3H11_1224 at 42.382 kb on - strand, within Ac3H11_1224 at 42.444 kb on + strand, within Ac3H11_1224 at 42.444 kb on + strand, within Ac3H11_1224 at 42.445 kb on - strand, within Ac3H11_1224 at 42.445 kb on - strand, within Ac3H11_1224 at 42.578 kb on + strand, within Ac3H11_1224 at 42.578 kb on + strand, within Ac3H11_1224 at 42.579 kb on - strand, within Ac3H11_1224 at 42.717 kb on + strand, within Ac3H11_1224 at 42.717 kb on + strand, within Ac3H11_1224 at 43.315 kb on - strand, within Ac3H11_1225 at 43.315 kb on - strand, within Ac3H11_1225 at 43.353 kb on + strand, within Ac3H11_1225 at 43.353 kb on + strand, within Ac3H11_1225 at 43.353 kb on + strand, within Ac3H11_1225 at 43.354 kb on - strand, within Ac3H11_1225 at 43.354 kb on - strand, within Ac3H11_1225 at 43.354 kb on - strand, within Ac3H11_1225 at 43.438 kb on - strand, within Ac3H11_1225 at 43.471 kb on - strand, within Ac3H11_1225 at 43.710 kb on + strand, within Ac3H11_1225
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 41,072 + Ac3H11_1222 0.33 -0.6 41,072 + Ac3H11_1222 0.33 -1.4 41,072 + Ac3H11_1222 0.33 +0.3 41,072 + Ac3H11_1222 0.33 -1.2 41,085 - Ac3H11_1222 0.34 -0.1 41,087 + Ac3H11_1222 0.35 -1.0 41,088 - Ac3H11_1222 0.35 -2.0 41,150 + Ac3H11_1222 0.43 +0.2 41,150 + Ac3H11_1222 0.43 -0.1 41,290 + Ac3H11_1222 0.61 -0.7 41,328 - Ac3H11_1222 0.66 -0.7 41,510 - Ac3H11_1222 0.90 -1.8 41,510 - Ac3H11_1222 0.90 -0.0 41,510 - Ac3H11_1222 0.90 -0.2 41,552 + +1.2 41,552 + -0.1 41,606 + -0.1 41,606 + -2.1 41,679 + Ac3H11_1223 0.19 -0.3 41,679 + Ac3H11_1223 0.19 -0.9 41,680 - Ac3H11_1223 0.19 -0.2 41,680 - Ac3H11_1223 0.19 +1.9 41,699 + Ac3H11_1223 0.23 +0.0 41,700 - Ac3H11_1223 0.23 -0.4 41,748 + Ac3H11_1223 0.34 +2.2 41,748 + Ac3H11_1223 0.34 +1.0 41,748 + Ac3H11_1223 0.34 -0.3 41,963 + Ac3H11_1223 0.81 +1.3 41,964 - Ac3H11_1223 0.81 +0.9 41,987 + Ac3H11_1223 0.86 +0.2 42,122 + +0.4 42,123 - -0.2 42,171 + Ac3H11_1224 0.11 +1.2 42,172 - Ac3H11_1224 0.11 -0.1 42,192 + Ac3H11_1224 0.14 -2.9 42,232 - Ac3H11_1224 0.18 +0.6 42,254 + Ac3H11_1224 0.21 -0.1 42,381 + Ac3H11_1224 0.35 +2.0 42,381 + Ac3H11_1224 0.35 +1.0 42,382 - Ac3H11_1224 0.35 -0.3 42,382 - Ac3H11_1224 0.35 +0.1 42,444 + Ac3H11_1224 0.42 +0.3 42,444 + Ac3H11_1224 0.42 +1.3 42,445 - Ac3H11_1224 0.42 -0.8 42,445 - Ac3H11_1224 0.42 -0.4 42,578 + Ac3H11_1224 0.56 +0.1 42,578 + Ac3H11_1224 0.56 +0.1 42,579 - Ac3H11_1224 0.56 +1.2 42,717 + Ac3H11_1224 0.72 +1.5 42,717 + Ac3H11_1224 0.72 -0.7 43,315 - Ac3H11_1225 0.36 +0.3 43,315 - Ac3H11_1225 0.36 -3.0 43,353 + Ac3H11_1225 0.40 +0.9 43,353 + Ac3H11_1225 0.40 -0.5 43,353 + Ac3H11_1225 0.40 +0.1 43,354 - Ac3H11_1225 0.41 -1.3 43,354 - Ac3H11_1225 0.41 +0.3 43,354 - Ac3H11_1225 0.41 -1.6 43,438 - Ac3H11_1225 0.50 -1.7 43,471 - Ac3H11_1225 0.54 -0.9 43,710 + Ac3H11_1225 0.80 -0.0
Or see this region's nucleotide sequence